Tuttnauer LABSCI 15LV User manual

1.1.1
Operation and Maintenance
Manual
Electronic Laboratory Autoclaves
Models LABSCI 15LV
ELV-D: standard autoclave
C: optional system for fast cooling
PV: optional vacuum pump
BH: optional bio hazard filtration
WR: water recycling system
F: optional fan for super-fast cooling
Cat. No. MAN205-0448007EN Rev. L
Heidolph North America,
1235 N Mittel Blvd. Suite B Wood Dale IL 60191
Tel: [phone] Fax: [phone] [email protected]

Page 1
Table of Contents
Paragraph Page no.
1. Generals................................................................................................................4
1.1 Introduction.................................................................................................4
1.2 Available optional configurations ...............................................................5
1.3 Incoming Inspection....................................................................................6
1.4 Warranty......................................................................................................6
1.5 Warranty Statement.....................................................................................7
1.6 Accessories...................................................................................................7
2. Safety Instructions................................................................................................8
3. Technical Data....................................................................................................10
3.1 Storage Conditions ....................................................................................10
3.2 Operating Conditions.................................................................................10
3.3 Directives and Standards...........................................................................10
3.4 Environment Emission Information .........................................................11
3.5 Electrical Data...........................................................................................11
3.6 Water Quality.............................................................................................12
3.7 Specifications.............................................................................................14
3.8 Construction ..............................................................................................14
3.9 Utilities.......................................................................................................15
3.10 Stickers Description...................................................................................16
3.11 Front View: Standard Configuration........................................................17
3.12 Front View: What is special for WR Configuration..................................18
3.13 Front View: What is Special for Fan Configuration ................................18
3.14 Rear View: Standard Configuration..........................................................19
3.15 Rear View: What is special for WR Configuration...................................20
3.16 Overall Dimensions: 3870ELVC-F-D, mm...............................................21
3.17 Overall Dimensions: 3870ELV-PRC-D, mm.............................................22
3.18 Overall Dimensions: 3870ELVC- WR-D Configuration, mm...................23
3.19 Safety Features..........................................................................................24
4. Control Panel......................................................................................................25
4.1 Control Panel Drawing..............................................................................25
4.2 Description and Functions of the Front Panel Keyboard.........................26
4.3 Displayed Error Messages / Symbols.........................................................28
4.4 Displayed operational messages / Symbols................................................28
5. Sterilization Programs........................................................................................30
5.1 Program 1: Glass.......................................................................................32
5.2 Program 2: Plastic.....................................................................................33

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5.3 Program 3: Liquid A..................................................................................34
5.4 Program 4: Liquid B –Waste....................................................................35
5.5 Program 5: Liquid A –Cooling (C –cooling models only).......................36
5.6 Program 6: Liquid B –Waste Cooling (C –cooling models only)............37
5.7 Program 7: Bio Hazard 1 (BH, bio-hazard models only) .........................39
5.8 Program 8: Bio Hazard 2 (BH, bio-hazard models only) .........................40
5.9 Program 9: Bio Hazard Liquids (BH, bio-hazard models only) ...............41
5.10 Program 10: Vacuum Test (PV, pre-vacuum models only) ......................42
5.11 Program 11: Warm-Up..............................................................................43
5.12 Program 12: Isothermal ............................................................................44
5.13 Program 13: Air Mixture...........................................................................46
5.14 Program 14: Glass Test .............................................................................47
5.15 Program 15: Durham ................................................................................48
6. Checking and Changing Parameters and Other Data .......................................50
6.1 Browsing through the menus ....................................................................50
6.2 Changing a Parameter...............................................................................51
6.3 Quick options screen..................................................................................53
6.4 Logging in and entering the Main menu ..................................................57
6.5 Changing parameters ................................................................................59
6.6 System Parameters.....................................................................................59
6.7 Maintenance ..............................................................................................60
6.8 Cycle parameters........................................................................................62
7. Screens................................................................................................................64
7.1 Screens following a complete successfully cycle –"Cycle Ended" ..........64
7.2 Screens following aborted cycles after complete sterilization stage..........64
7.3 Screens following a fail cycle:...................................................................65
8. Printer.................................................................................................................67
8.1 Printer Output............................................................................................67
8.2 Printer Handling........................................................................................70
9. Preparation before Sterilization .........................................................................73
9.1 Instruments................................................................................................73
9.2 Liquids .......................................................................................................74
9.3 Loading......................................................................................................74
10. Operating Instructions........................................................................................76
10.1 Draining and filling the water reservoir (WR only)..................................76
10.2 Turning on the autoclave ..........................................................................77
10.3 Opening the door .......................................................................................78
10.4 Loading......................................................................................................79
10.5 Closing and locking the door.....................................................................79
10.6 Starting the cycle .......................................................................................81
10.7 Unloading ..................................................................................................82

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10.8 Stopping the process and cancelling the ERROR message.......................82
10.9 Start Cycle by Clock mode.........................................................................83
10.10 Moving the Autoclave................................................................................83
10.11 Loading and Unloading the Device...........................................................83
11. Service and Maintenance ...................................................................................85
11.1 Preventive Maintenance ............................................................................85
11.2 Checking the Safety Valve.........................................................................86
12. Troubleshooting..................................................................................................89
13. Baskets and Containers ......................................................................................94
14. Spare Parts List...................................................................................................95
15. Accessories..........................................................................................................95

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1. Generals
1.1 Introduction
A computerized control unit ensuring a fully automatic sterilization cycle
controls the autoclave.
The temperature and pressure are controlled through sensors placed
inside and outside the media container or bottles.
The sterilizer is fully automatic with a choice of 10 programs,
eliminating any need for operator intervention during a cycle program 7
are for vacuum test only). A computerized control unit enables precise
control and monitoring of physical parameters and clear documentation
of the sterilization cycles.
The autoclave is equipped with a safety valve, which will discharge at
an overpressure of over 2.8 Bar (40 psi), which is located on the
chamber near the pressure switch and the steam pressure gauge. The
control system provides adequate protection, to ensure the safety of
personnel and reliable operation with a minimum of down time.
The sterilizer has multiple built - in safety devices, which provides
adequate protection to ensure the safety of operating personnel.
On all models, a printer is an optional addition to the autoclave. The
printer prints the preset and actual parameters of the cycle
(temperature, time and pressure).
The autoclave is provided with a pressure gauge that is used as guide
only. Should there be a power failure during the operation of the
autoclave, the pressure gauge indicates to the operator that there is
pressure in the chamber.
A deviation of +1.6% is accepted.
Note: After operating the sterilizer, brown stains might appear on the bottom
of the chamber. These stains are a result of the heating elements that
are located at the lower external part of the chamber. The brown color
is a common phenomenon, can easily be removed, and will not have
any effect on the sterilized goods.
This manual is intended to give the user a general understanding of
how the autoclave works and indicates the best ways to operate and

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take care of it in order to obtain optimum results and a trouble-free
operation. After reading this manual, operating the autoclave should be
straightforward. However, since the autoclave is built using high
technology sensitive components, no attempt should be made by the
user or any other unauthorized person to repair or recalibrate it.
Only technical personnel having proper qualifications, holding
technical documentation and adequate test instrumentation are
authorized to undertake repair or service.
1.2 Available optional configurations
Models of LABSCI 15LV are laboratory sterilizers designed especially
for the sterilization of instruments, liquids, and other materials in
hospital laboratories, laboratories & research institutes, food
laboratories and pharmaceutical facilities.
C
A special feature of the ELVC-D models is the fast cooling stage for
liquids. In this stage pressure in the chamber is increased by means of
compressed air to compensate the fast decreasing of pressure due to
the fast cooling. The fast cooling shortens the time required for safe
handling of bottles.
Note: there is no cooling option for WR configuration
PV
Special feature of ELVCPV-D models is the vacuum pump that enables
air removal prior to sterilization and during the drying stage.
The advantages of the pre-vacuum sterilizer in comparison to the
regular gravity displacement steam sterilizer are as follows:
—Removal of air pockets from packs and porous loads and most kinds of
tubes (rubber, plastic etc.) by vacuum at the first stage of the cycle.
—Better steam penetration into the load; resulting in effective
sterilization.
—Better temperature uniformity.
—Better drying of materials with closed doors due to the vacuum
achieved in the chamber at the end of the sterilization cycle.
A special feature of the autoclave is the fast cooling stage for liquids. In
this stage pressure in the chamber is increased by means of
compressed air to compensate the fast decreasing of pressure due to
the fast cooling. The fast cooling shortens the time required for safe
handling of bottles

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There is a configuration equipped with fan that allows to shorten the
cooling operation.
BH
In ELVCPVBH-D (bio-hazard) models, a bio-hazard filter installed in
the chamber enables sterilizing bio-hazard load without contaminating
the surrounding.
WR
In ELVWR-D (Water recycling) models, water is saved by the water
saving system, because it recycles the water and re-uses it for each
cycle in the following way:
•Water inlet by the pump from the reservoir and not direct from the
plumbing.
•On slow/fast exhaust, the water gets into the reservoir.
1.3 Incoming Inspection
The autoclave should be unpacked and inspected for mechanical
damage upon receipt. Observe packing method and retain packing
materials until the unit has been inspected. Mechanical inspection
involves checking for signs of physical damage such as: scratched
panel surfaces, broken knobs, etc.
If damage is apparent, contact your dealer or point of purchase, so that
they may notify the manufacturer and file a claim with the appropriate
carrier.
All Tuttnauer products are carefully inspected prior to shipment and all
reasonable precautions are taken in preparing them for shipment to
assure safe arrival at their destination.
1.4 Warranty
We certify that this instrument is guaranteed to be free from defects in
material and workmanship for one year against faulty components and
assembly with the exception of glassware, lamps and heaters.
The warranty does not include and does not replace routine
treatment and preventive maintenance to be performed according
to instructions in sec. 12.1 (Preventive Maintenance).
Our obligation is limited to replacing the instrument or parts, after our
examination, if within one year after the date of shipment they prove to
be defective. This warranty does not apply to any instrument that has
been subjected to misuse, neglect, accident or improper installation or
application, nor shall it extend to products that have been repaired or
altered outside the factory without prior authorization from us.
The Autoclave should not be used in a manner not described in
this manual!

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1.5 Warranty Statement
The warranty registration must be completed and returned to our
service departments; within fourteen (14) days of purchase or the
warranty will be void.
Our Technical Service Depts can be reached at:
Heidolph North America, 1235 N Mittel Blvd. Suite B Wood Dale
IL 60191
Service & Repair Division
Troubleshooting, Service incidents, Warranty repair, Repair, Services
and Return authorization
224-265-9600 Fax: [phone], e-mail: [email protected]
Tuttnauer USA Co., Ltd., 25 Power Drive Hauppauge, NY 11788,
USA : [phone], [phone], Fax: [phone]
e-mail: [email protected].
Note:
If there is any difficulty with this instrument, and the solution is not
covered in this manual, contact our representative or us first. Do not
attempt to service this instrument yourself. Describe the difficulty as
clearly as possible so we may be able to diagnose the problem and
provide a prompt solution.
If the autoclave is equipped with a printer, send along a copy of the last
printout for our inspection. If replacement parts are needed, stipulate
the model and serial number of the machine.
No products will be accepted for repair without proper authorization
from us. All transportation charges must be paid both ways by the
owner. This warranty will be void if the unit is not purchased from an
authorized full service Tuttnauer dealer.
1.6 Accessories
Basket accessories: A set of two baskets is available for the unit. The
baskets are made of stainless steel wire and have a handle. The
basket allows the operator to load a large quantity of materials into the
chamber.
The pressure scale, printer option and cooling method can be set up at
any time by a technician.
Stainless steel containers: A stainless steel container, for waste
products, is available. This container has vent holes on its upper part

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2. Safety Instructions
The autoclave has unique characteristics. Please read and understand
the operation instructions before first operation of the autoclave. The
following issues may require instructions guidance provided by the
manufacturer: how to operate the autoclave, the door safety
mechanism, the dangers involved in circumventing safety means, how
to ensure that the door is closed, and how to select a correct
sterilization program.
Make sure that you know where the main power switch is, where the
water cut-off valve is and where the compressed air disconnection
valves (if applicable) are located.
Autoclave maintenance is crucial for the correct and efficient function of
the device. We enclose a log booklet that includes maintenance
recommendations, with every device.
The weekly spore test is part of the preventive maintenance plan, along
with the annual validation of the sterilization processes that ensures
appropriate temperature dispersion within the chamber.
Never use the autoclave to sterilize corrosive products, such as: acids,
bases and phenols, volatile compounds or solutions such ethanol,
methanol or chloroform nor radioactive substances.
1. Never start using a new autoclave before the safety, licensing and
authorization department has approved it for use.
2. All autoclave users must receive training in proper usage from an
experienced employee. Every new employee must undergo a training
period under an experienced employee.
3. A written procedure must be established for autoclave operation,
including: daily safety tests, seal inspection and door hinge
inspection, smooth action of the closing mechanism, chamber
cleaning, prevention of clogging and preservation from corrosion,
what is permitted and what is prohibited for sterilization and choosing
a sterilization program.
4. Liquids may be sterilized only with the “liquids” programs. The
container must be covered but not sealed. Sealed bottles may only be
sterilized using a special program. The bottle must be either Pyrex or
a Borosilicate glass bottle. Verify that the two temperature sensors are
located inside two different bottles to assure that the liquid temperature
is 20°C below boiling temperature for sealed bottles at the end of the
cycle.
5. When sterilizing plastic materials, make sure that the item can
withstand sterilization temperature. Plastic that melts in the chamber
is liable to cause a great deal of damage.
6. Individual glass bottles may be placed within an appropriate container
that will be placed in a basket. Never place glass bottles on the floor
of the autoclave. Never fill more than 2/3 of the bottle volume.

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7. On closing the autoclave's door, make sure it is properly locked
before activating.
8. Before withdrawing baskets, wear heat resistant gloves.
9. Before opening the door, verify that there is no pressure in the
chamber (chamber pressure gauge is located on the autoclave's front
panel or door, depends on model).
10. Open the door slowly to allow steam to escape and wait 5 minutes
before you remove the load. When sterilizing liquids, wait 10 minutes.
11. Once a month, ensure that the safety valves are functioning, and
once annually a certified tester must conduct pressure chamber
safety tests.
12. Once annually, or more frequently, effective tests must be performed,
i.e., calibration and validation.
13. Examine the condition of assemblies on a regular basis. Make sure
there are no leaks, breaks, blockages, whistles or strange noises.
14. It is required to conduct maintenance operations as instructed.
15. Immediately notify the person in charge of any deviation or risk for the
proper function of the device.
16. For WR models, drain the water from the mineral free water reservoir
once a week, and refill with fresh mineral-free water or distilled water
(see 10.1).
17. This autoclave equipped with rear and front wheels, when moving the
autoclave all utilities must be disconnected, at least 2 persons shall
move the autoclave with caution. When the autoclave located in the
required place, the wheels have to be locked. For further information
see the front/rear view of the autoclave.

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3. Technical Data
3.1 Storage Conditions
The packed or unpacked autoclave shall be retained in indoor
conditions!
3.2 Operating Conditions
▪The autoclave is intended to work in ‘indoor’ conditions only.
▪Only autoclavable materials shall be used.
•The environment shall not exceed an ambient temperature range of
5ºC (41ºF)-40ºC (104ºF) and a relative humidity of 85% respectively.
•The operation altitude shall not be over 2000 meters (6561 feet)
(ambient pressure shall not be lower than 80 kPa (11.6 psi)).
•The autoclave shall not be used in a manner not specified in this
manual!
•Do not use the autoclave in the presence of dangerous gases.
•The packed or unpacked device shall be stored in ‘indoor’ conditions.
•Operate the autoclave only in the manner specified in the manual. If
the equipment is used in a manner not specified by the manufacturer,
the protection provided by the equipment may be impaired.
Caution!
Waste water should be brought into the public net in accordance
with the local rules or requirements i.e. only non-hazardous
liquids shall be disposed in public sewage!
3.3 Directives and Standards
EN ISO 13485:2012 (Quality Systems for Devices)
ISO 9001:2008 (Quality Systems)
MDD 93/42/EEC (Device Directive)
Tuttnauer. Ltd. company also works in conjunction with and refers to:
AAMI/ANSI-ST8 Hospital sterilizers

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ASME American Society of Mechanical Engineers
Section VIII, Division 1, for unfired pressure vessels
EN 13060 Small Steam Sterilizers
UL UL 61010-1
PED 97/23EEC
IEC IEC 61010-2-040 Safety
IEC IEC 61326-1 EMC
ISO 17665-1:2006 (Validation and Routine Control)
3.3.1 Quality standards
The manufacturing plant meets the following quality standards:
1. EN ISO 9001:2008–Quality System
2. ISO 13485:2003 –Quality systems –Devices.
3.4 Environment Emission Information
A. Peak sound level generated by the sterilizer is « 70 / dBA with a back
sound level of 60 dBA.
B. Total heat transmitted by the sterilizer is < 600 W/h
3.5 Electrical Data
230V (WR only) configuration
Property
Value: LABSCI 15LV
Total Power
3*2000W
Voltage
3Ph, 230VAC
Amperage
15A
Protection against electrical
shock
Class I (IEC 60601-1)
Mains supply fluctuation
+/- 10%
Degree of protection by
enclosure
IP31

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400V configuration
Property
Value: LABSCI 15LV
Total Power
6000W (3*2000W)
Voltage
3Ph, 400VAC
Amperage
9A
Protection against electrical
shock
Class I (IEC 60601-1)
Mains supply fluctuation
+/- 10%
Degree of protection by
enclosure
IP31
3.6 Water Quality
The distilled or mineral-free water supply shall be according to the table
below:
Physical Characteristics and Maximum acceptable contaminants levels
in water or steam, for steam generator and sterilizers
Contaminants in
water supplied to
generator/chamber
Contaminants in
condensate at steam
inlet to sterilizer
Evaporate residue
≤ 10 mg/l
N/A
Silicate (SiO2)
≤ 1 mg/l
≤ 0.1 mg/l
Iron
≤ 0.2mg/l
≤ 0.1mg/l
Cadmium
≤ 0.005 mg/l
≤ 0.005 mg/l
Lead
≤ 0.05 mg/l
≤ 0.05 mg/l
Rest of heavy metals
except iron,
cadmium, lead
≤ 0.1 mg/l
≤ 0.1 mg/l
Chloride (Cl)
≤ 2 mg/l
≤ 0.1 mg/l
Phosphate (P2O5)
≤ 0.5 mg/l
≤ 0.1 mg/l
Conductivity (at
25°C)
3 to 15 µs/cm
≤ 3 µs/cm
pH value (degree of
acidity)
5 to 7.5
5 to 7
Hardness (Σ ions of
alkaline earth)
≤ 0.02 mmol/l
≤ 0.02 mmol/l
Appearance
Colorless, clean, without sediments

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Compliance with the above data should be tested in accordance with
acknowledged analytical methods, by an authorized laboratory.
Attention:
The use of water for autoclaves that do not comply with the table
above may have severe impact on the working life of the sterilizer
and can invalidate the manufacturer’s guarantee.
Use only deionized water, having a maximum conductivity of 15
µs/cm. Conductivity greater than 15 µs/cm may cause failures.
Tap water supply
The range of hardness value 0.7-2.0 mmol/l (70- 200 mg/l CaCO3)
The use of soft water is strictly forbidden!
Please consult a water specialist!
3.6.1 Reverse Osmosis
A Reverse Osmosis (RO) system may be used to improve the quality
of the water used to generate steam in the autoclave chamber.
In RO, the water is forced through a semi-penetrable membrane, which
filters out contaminants to a high degree of efficiency. In deionisation
(DI) ions and charged particles are removed either by electric fields or
by ion exchange in resin beds.
Although the RO cannot normally attain the degree of purity possible
with the DI methods, it is more than adequate for the feed water
intended for clean-steam generators.
Moreover the RO has several advantages:
1. RO is cheaper to install and to run than DI.
2. RO removes particulate matter, organic molecules and pyrogens that
DI cannot remove
3. RO water is less corrosive to steel and copper than DI water.
4. RO maintenance requirements are less demanding than those of the
DI units.
Therefore the use of mineral free water will contribute to better
performance and longer life of the autoclave.

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3.7 Specifications
Model
Property
LABSCI 15LV
Overall
dimensions
(standard
configuration)
Height
1000mm
Width
730mm
Length
540mm
Overall
dimensions
(WR
configuration)
Height
1080mm
Width
730mm
Length
7300mm
Maximum
dimensions
(door open)
Width
800mm
Height
1500mm
Net weight
113kg
Weight per support area
(max. load)
According to overall weight and floor loading
requirements
Shipping volume
0.9 m3
Shipping weight
141kg
Shipping
dimensions
length
900mm
width
730mm
height
1350mm
Max. Allowable Working
pressure (MAWP)
2.8 bar
Chamber
diameter
380mm
Depth
690mm
Chamber Volume
85L
3.8 Construction
Chamber and door material
Stainless steel
Outer Cabinet
Stainless Steel
Chamber insulation
Fiberglass with reinforced material
Water reservoir
Stainless Steel

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3.9 Utilities
3.9.1 Electrical utility
Power
Switch
box
Recommended
Circuit Breaker
208-220V (3 Ph + Earthing)
No
20A
400V(3 Ph + Neutral +
Earthing)
No
16A
230V 1ph
No
16A
Frequency
50/60Hz
3.9.2 Other Utilities
Compressed Air (C,
cooling models only)
1/2", 3 Bar (44-58 psi)
Tap water
1/2", 2-6 Bar (29-44 psi)
Mineral free water
1/2", 2-3 Bar (29-44 psi)
Drain
2" Minimum Withstanding
temp. of 80°C (176°F)
Attention:
•A switch or circuit-breaker must be included in the building
installation. This switch or circuit-breaker shall be in close
proximity to the equipment, within easy reach of the operator; and
marked as the disconnecting device for the equipment.
•The electrical net must be protected with a current leakage safety
relay.
•The electrical network must comply with local rules or
regulations.
•Verify that there is an easy access to the main power switch, to
the water cut-off valve and to the current leakage safety relay.
•Make sure while placing the autoclave, to leave space around the
machine, to give the technician access to service the machine.
•All water connections to autoclave must be performed through
"BACK FLOW PREVENTION SYSTEM" only, as per IEC 61770.

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3.10 Stickers Description
Symbol
Meaning
Part Number
Location
Caution! Hot
steam.
LAB048-0058
Near the safety
valve
Protective earth
(Ground)
LAB048-0020
Near the earthing
screw
Caution! Hot
surface.
LAB048-0023
On the rear cover
above the water
strainer
Press & Hold
Until “System
Ready” is
Displayed = 10
sec.
LAB048-0461
(Automatic
door only)
On the door right
edge
Don’t Touch
Microswitches
please Contact
Tuttnauer
Service
LAB048-0462
On the door frame
behind the door
cover
Don’t Touch
Microswitches
please Contact
Tuttnauer
Service
LAB048-0463
On the door frame
behind the door
cover
ON
OFF
On-Off
LAB048-0018
Near the Power
switch

Page 17
3.11 Front View: Standard Configuration
No.
Description
1
Front leg
8
Display
2
Rear leg
9
Keyboard
3
Left service door
10
Printer cover
4
Left service door grip
11
Paper slot and paper cutter
5
Left service door lock
12
USB socket
6
Door cover
13
Main switch
7
Pressure gauge
14
Printer
9
10
5
6
7
8
11
14
4
3
2
1
12
13

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3.12 Front View: What is special for WR Configuration
No.
Description
1
Glass tube indicator of the reservoir water level
2
Inlet for manual water reservoir filling
3.13 Front View: What is Special for Fan Configuration
There is a configuration equipped with a fan that allows to shorten the
cooling operation. The fan is located on the door of the autoclave.
2
1
Fan

Page 19
3.14 Rear View: Standard Configuration
No.
Description
No.
Description
1
Drain outlet
8
Safety Valve
2
Mineral free water inlet
9
Circuit breaker
3
Compressed air inlet
10
Ground connector
4
Cavitation port
11
City (tap) water inlet for cooling coil
5
Power supply electrical
cord
12
Air pressure gauge
6
Cut-off
13
Air pressure regulator
7
Fan grill
5
6
4
3
2
1
12
13
10
11
7
8
9