Star Lab ErgoOne E Series User manual

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USER MANUAL · BEDIENUNGSANLEITUNG
MODE D‘EMPLOI · ISTRUZIONI PER L‘USO · INSTRUCCIONES DE MANEJO
This is your ErgoOne® E
Multi-Channel Pipettes
user manual.

2 · ErgoOne®E Multi-Channel Pipettes · User Manual

ErgoOne® E Multi-Channel Pipettes · User Manual · 3
English
Table of contents
User Manual ____________________________________________________________________ 3 – 40
Bedienungsanleitung__________________________________________________________ 41 – 78
Mode d’emploi ________________________________________________________________ 79 – 116
Istruzioni d’usoA ______________________________________________________________117 – 154
Instrucciones de manejo Istruzioni d’uso ____________________________________ 155 – 192
1. Introduction _________________________________________________________________ 5
1.1 Scope of delivery _______________________________________________________ 5
1.2 Terms of use ___________________________________________________________ 5
1.2.1 Hazard levels __________________________________________________________ 5
1.2.2 Format _______________________________________________________________ 5
1.2.3 Operating manual symbols________________________________________________ 5
2. Safetyregulations ___________________________________________________________ 6
2.1 General safety regulations ________________________________________________ 6
2.2 Intended use___________________________________________________________ 7
2.3 Limitations of use _______________________________________________________ 7
2.4 Application restrictions __________________________________________________ 7
2.5 Operating exclusions ____________________________________________________ 7
2.6 Rechargeablebatteryandpoweradapterspecications_________________________ 7
3. Functionalandoperationalcomponents_____________________________________8
4. Operation ____________________________________________________________________ 9
4.1
4.2
4.3
4.4
4.5
First Steps_____________________________________________________________ 9
Set volume ___________________________________________________________ 10
Set the aspiration and dispensing speed ____________________________________ 11
Pipetting _____________________________________________________________ 12
X Direct trigger blow-out___________________________________________________ 13
5. Pipettingprograms_________________________________________________________ 14
5.1 PIP mode ____________________________________________________________ 14
5.2 PIPmix mode _________________________________________________________ 15
5.3 revPIP mode __________________________________________________________ 16
5.4 Electrophoresis (GEL) mode______________________________________________ 18
5.5 DISP mode ___________________________________________________________ 19
6. Checkingthevolume_______________________________________________________ 21
7. Accuracytable______________________________________________________________ 22
8. Adjustment–EasyCalibration _____________________________________________ 23
8.1 Adjustment ___________________________________________________________ 23
8.2 Restore factory setting __________________________________________________ 24

4·ErgoOne®E Multi-Channel Pipettes · User Manual
9. Disinfection/autoclaving ___________________________________________________24
9.1 UV sterilization ________________________________________________________ 24
9.2 Autoclaving___________________________________________________________ 25
9.3 Reference run (rEF)_____________________________________________________ 25
10. Maintenance________________________________________________________________ 25
10.1 Separate the pipetting unit from the handle__________________________________ 26
10.2 Disassembly of ErgoOne®E Multi-Channel pipettes up to 300 µl _________________ 26
10.3 Disassembly of ErgoOne®E Multi-Channel pipettes 1250 µl_____________________ 29
10.4 Charge and replace rechargeable battery ___________________________________ 33
10.5 Rechargeable battery regeneration function _________________________________ 34
11. Troubleshooting _____________________________________________________ 35
12. Productmarkings ___________________________________________________ 36
13. Order Information ___________________________________________________ 37
13.1 Ordering Information ___________________________________________________ 37
13.2 Spares_______________________________________________________________ 37
13.2.1 ErgoOne® E Multi-channel pipettes to 300 µl_________________________________ 37
13.2.2 ErgoOne® E Multi-Channel pipette 1250 µl __________________________________ 38
13.3 Additional accessories __________________________________________________ 38
14. Repairs _____________________________________________________________ 39
14.1 Sending for repair ______________________________________________________ 39
15. Calibrationservice___________________________________________________ 39
16. Warrantyandguarantee _____________________________________________ 39
17. Disposal ____________________________________________________________ 40

ErgoOne® EMulti-ChannelPipettes·UserManual·5
1. Introduction
1.1 Scopeofdelivery
The ErgoOne® E Multi-Channel pipette, one battery pack, one reagent reservoir, grease, operating
manual.
Nominal volume Scope of delivery
Up to 300 µl Plug-in power supply unit with battery charging cable, 1 instrument stand,
1 set of O-rings made of FKM/silicone, 1 TipOne® pipette tip rack
1250 µl Universal power adapter with battery charging cable, 1 shelf mount,
1 set of O-rings made of silicone, 2 TipOne® pipette tip racks
1.2 Termsofuse
› Carefullyreadtheoperatingmanualbeforeusingthedeviceforthersttime.
› The operating manual is part of the device and must be kept in an easily accessible
place.
› Be sure to include the operating manual if you transfer possession of this device to
a third party.
1.2.1 Hazardlevels
The following signal words identify possible hazards:
Signal word Meaning
DANGER Will lead to serious injury or death.
WARNING May lead to serious injury or death.
CAUTION May lead to minor or moderate injuries.
NOTICE May lead to property damage.
1.2.2 Format
Format Meaning Format Meaning
1. Task Indicates a task. > Indicates a
condition.
a., b., c. Indicates the individual steps of a task. Indicates a result.
1.2.3 Operatingmanualsymbols
Icon Meaning Icon Meaning
Hazardous, may cause
moderate injuries.
Explosion hazard
Introduction English

6·ErgoOne®E Multi-Channel Pipettes · User Manual
2. Safetyregulations
2.1 Generalsafetyregulations
Please read carefully!
The instrument ErgoOne® E Multi-Channel pipette can be used in combination with hazardous
materials, work processes and equipment. However, the operating manual cannot cover all of the
safety issues that may occur in doing so. It is the user’s responsibility to ensure compliance with
the safety and health regulations and to specify the corresponding restrictions before use.
1. Every user must read and understand this operating manual before operation.
2. Follow the general hazard instructions and safety regulations, e.g. wear protective
clothing, eye protection and protective gloves.
When working with infectious or hazardous samples, standard laboratory rules
and precautions must be adhered to.
3. Observeallspecificationsprovidedbythereagentmanufacturer.
4. Do not operate the instrument in potentially explosive atmospheres and do not
pipette highly flammable media.
5. Usetheinstrumentonlyforpipettingliquidswithinthedefinedlimitationsand
restrictions of use. Comply with the operating exclusions; see Operating exclusions. If
in doubt, contact the manufacturer or supplier.
6. Always perform work in a way that does not endanger the user or any other person.
Avoid splashes. Use only suitable vessels.
7. Avoid touching the tip opening when working with aggressive media.
8. Never use force.
9. Useonlyoriginalspareparts.Donotattempttomakeanytechnicalmodifications. Do
not disassemble the instrument any further than is described in the operating manual.
10. Always check that the instrument is in proper working condition before use. If there
are any signs of the instrument malfunctioning (e.g., sluggish piston, leakage), stop
pipetting immediately, and follow the instructions in the chapter Troubleshooting.
Contact the manufacturer, if necessary.
11. The original rechargeable battery may not be exchanged for batteries or rechargeable
batteries from other manufacturers.
12. Use only the original power adapter to charge the nickel-metal hydride battery.
13. Protect the power adapter from moisture and use it only with this instrument.
14. Dispose of batteries according to the applicable regulations.
Potential risk of explosion due to damaged battery
In extreme cases, improper handling of the instrument or the battery (short circuit,
mechanical damage, overheating, etc.) may cause the battery to explode.
WARNING!

ErgoOne® EMulti-ChannelPipettes·UserManual·7
SafetyInstructions English
2.2 Intended use
ErgoOne® E Multi-Channel is a microprocessor-controlled, battery-operated piston-stroke
pipette based on the air cushion principle for pipetting aqueous solutions of medium density and
viscosity. If the instrument is handled correctly, the sample to be dispensed comes into contact
only with the tip and not with the ErgoOne® E Multi-Channel.
2.3 Limitations of use
This instrument is intended for pipetting samples, within the following limitations:
› Operating temperature of instrument and reagent should be between +15 °C and
+40 °C (59 °F to 104 °F) (other temperatures upon request)
› Vapor pressure up to 500 mbar
› Viscosity: 260 mPas
For viscous media, the speed must be adjusted if necessary.
2.4 Application restrictions
Viscous and wetting liquids may compromise volumetric accuracy. Volumetric accuracy may also
be affected when pipetting liquids whose temperature deviates from the ambient temperature by
more than ± 1 °C/± 1.8 °F.
2.5 Operating exclusions
The user is responsible for checking the compatibility of the device with the intended application.
The device cannot be used:
› for liquids that attack PP, FKM, PVDF,SI, PEI or PC/PBT. Avoid aggressive vapors
(risk of corrosion).
The handle cannot be autoclaved.
2.6 Rechargeablebatteryandpoweradapterspecications
Rechargeable battery
Nickel metal hydride rechargeable battery with 3 cylindrical single cells size AAA, 3.6 V, 700 mAh
Power adapter
Output voltage 6.5 V DC, 200 mA

8·ErgoOne®E Multi-Channel Pipettes · User Manual
3. Functionalandoperationalcomponents
1Charging socket 2Battery compartment
3Menu selection key 4Arrow key (+)
5‘Enter’ key
Inputconrmation/Power‘On’*)
6Arrowkey(−)
7Finger rest 8Tip cone
9Pipetting unit 10 Handle
11 Tip ejection key 12 Pipetting key
13 Display
*)Theinstrumentisswitchedonbypressingthe‘Enter’key.Bysubsequentlypressingthe
pipetting key, the instrument is ready for pipetting.
The ErgoOne® E Multi-Channel pipette switches itself off automatically 10 minutes after the
lastoperation(autopoweroff).TheErgoOne®EMulti-Channelpipettetsergonomicallyinyour
hand. For absolutely effortless operation of the function keys, the hand position can be further
optimizedbymeansofthengergrip,whichisheight-adjustableviaascrew.
1
13
12
11
10
9
8
2
7
345
6

ErgoOne® EMulti-ChannelPipettes·UserManual·9
1Charging socket 2Battery compartment
3Menu selection key 4Arrow key (+)
5‘Enter’ key
Input conrmation/Power ‘On’*)
6Arrow key (−)
7Finger rest 8Tip cone
9Pipetting unit 10 Handle
11 Tip ejection key 12 Pipetting key
13 Display
English
Functionalandoperationalcomponents/Operation
4. Operation
4.1 FirstSteps
1. Insert rechargeable battery
a. Open the cover of the battery compartment.
b. Insert the rechargeable battery into the
compartment. Make sure that the plug of the
rechargeablebatteryisrmlyinsertedintothe
socket in the instrument.
c. Replace the cover of the battery compartment and
close it.
2. Activate instrument
The ErgoOne® E Multi-Channel pipette automatically
requests a reference run immediately after the battery
is inserted. After pressing the pipetting key, the
reference run is carried out, and the instrument is ready
for pipetting.
The display shows the factory-set default pipetting
mode (PIP) and the respective nominal volume. The
aspiration and dispensing speed are set to maximum
at the factory.
The simple volume and speed setting is described on
the following pages.
Rechargetable
battery capacity
indicator
Arrow icon
‘Aspirate’
Pipetting
Volume
display
c.
b.
a.
1x

10·ErgoOne®E Multi-Channel Pipettes · User Manual
4.2 Setvolume
The volume is factory-set to the respective nominal volume of the ErgoOne® E Multi-Channel
pipette and can be easily and quickly changed individually.
a. Pressing one of the arrow keys directly selects
a volume.
‘VOL’ flashes.
b. Pressingthearrowkey(−)decreasesthe
volume. Pressing and holding the arrow key
causes the volume to change quickly.
‘VOL’ continues to flash.
c. Pressing the arrow key (+) increases the volume.
Pressing and holding the arrow key causes the
volume to change quickly.
VOL’ continues to flash.
d. Toconrmthevolumeselection,pressthe
‘Enter’ key.
The display now shows the newly set volume.
Here, for example, the display of the PIP mode
set by default.
NOTICE!
By pressing the menu key, any setting procedure can be canceled. The display then jumps to the
next setting option or back to the initial display.
–
+
1x

ErgoOne® EMulti-ChannelPipettes·UserManual·11
English
Operation
4.3 Settheaspirationanddispensingspeed
Aspiration and dispensing speed are separately adjustable. When the menu is called up, the last
speed set in each case is displayed. Five speed levels are available in each case.
Set aspiration speed
a. Pressing the menu key once briefly takes you to
the aspiration speed menu.
‘Speed’ flashes.
b. Pressthearrowkeys(+\−)toselectthespeed
level (e.g. level 5).
‘Speed’ continues to flash.
c. Press ‘Enter’ key.
The display returns to the basic state of the
respective set mode. Here, for example the
display of the standard PIP mode.
Set dispensing speed
a. Pressing the menu key twice briefly takes you to
the dispensing speed menu.
‘Speed’ flashes.
b. Pressthearrowkeys(+\−)toselectthespeedlevel
(e.g. level 2).
‘Speed’ continues to flash.
c. Press ‘Enter’ key.
The display returns to the basic state of the
respective set mode. Here, for example the
display of the standard PIP mode.
1x
+
–
1x
1x
2x
+
–

12·ErgoOne®E Multi-Channel Pipettes · User Manual
4.4 Pipetting
The volume is factory-set to the respective nominal volume of the ErgoOne® E Multi-Channel
pipette and can be easily and quickly changed individually, see Set volume.
NOTICE!
› Perfect analysis results can only be achieved by using quality tips. We recommend the TipOne®
tips which are delivered with the pipette or other compatible pipette tips, see TipFinder on
Starlab’s website www.starlabgroup.com.
›Ifotherpipettetipsareused,checkwhetherthesetipsttheErgoOne®EMulti-Channel
pipette and have the required quality for the desired application.
a. Insert tip vertically:
Use the correct tips, in accordance with the volume
range or color code!
Makesurethatthetipsarermlyinplaceandleak
tight. Pipette tips are disposable products!
b. Align pipetting unit: The pipetting unit can turn
freely in both directions.
c. Aspirate liquid: Hold the instrument vertically and
immerse the tip in the liquid. The liquid is aspirated
by pressing the pipetting key. The arrow in the
display points upwards (aspiration).
Leave the tip immersed in the liquid for a few
seconds, so that the set volume is aspirated
completely. This is especially important when
pipetting viscous media and when using pipettes
with large volumes.
Volume range Immersion depth Waittime
0.5 – 100 µl 2 – 3 mm 1 s
100 – 300 µl 2 – 4 mm 1 s
> 1000 µl 3 – 6 mm 3 s
1x

ErgoOne® EMulti-ChannelPipettes·UserManual·13
English
Operation
d. Dispense liquid: When the liquid aspiration is
nished,thearrowinthedisplaypointsdownwards
(dispensing). Place the pipette tip against the
vessel wall. Hold the pipette at an angle of 30–45°.
By pressing the pipetting key again, the liquid is
dispensed completely with automatic blow-out
While doing this, wipe the pipette tip against the
vessel wall.
e. Ejecting a tip: Hold the pipette Pipetting unit over
a suitable disposal container and the press the tip
ejection key.
NOTICE!
ISO 8655 requires that pipette tips are pre-wetted once before the actual pipetting procedure.
4.5 Directtriggerblow-out
The blow-out can also be triggered directly at any time if necessary.
a. Call up the blow-out function: Press ‘Enter’ key.
The display shows ‘blo’ for blow-out.
b. Trigger blow-out: Press the pipetting key once
to trigger the blow-out. The display will then
jump back to the set pipetting mode (start
position).
NOTICE!
During blow-out, the piston moves completely downwards. Ensure that any residual liquid is
discharged safely. Keeping the pipetting key pressed keeps the piston down and thus prevents
accidental aspiration of liquid. Releasing causes the piston to return to the start position.
1x
1x
1x

14·ErgoOne®E Multi-Channel Pipettes · User Manual
5. Pipetting programs
Activity Info
Normal
pipetting
Standard program.
A previously entered volume is aspirated and
dispensed again
Pipetting for
electro-
phoresis
Program for loading electrophoresis gels. A previously
denedsamplevolumeisaspiratedatahigh,variable
speed and slowly released again.
Mixing
samples
Program for mixing liquids.
Sample is aspirated and dispensed repeatedly.
Reverse
pipetting
Program especially for pipetting liquids with high
viscosity, high vapor pressure, or foaming media.
Dispensing
Designation
PIP mode, see PIP
mode
GEL mode, see
Electrophoresis (GEL)
mode
PIPmix mode, see
PIPmix mode
revPIP mode, see
revPIP mode
DISP mode, see
DISP mode
Program for dispensing liquids.
A volume taken up is dispensed again in partial steps.
NOTICE!
GEL mode
TheGELmodeisnotavailableforErgoOne®E1000μland5000μlMulti-Channelpipettes.
5.1 PIP mode
The default program – a previously entered volume is aspirated and dispensed again.
Volume and speed setting, see Set volume and Set the aspiration and dispensing speed.
a. Call up menu selection: Pressing the menu key
three times takes you to the program selection.
‘Mode’ flashes.
b. Set PIP mode: Use one of the arrow keys to scroll
through the modes until ‘PIP’ appears.
‘Mode’ continues to flash.
3x

ErgoOne® EMulti-ChannelPipettes·UserManual·15
English
Pipetting programs
c. ConrmPIPmode:Press‘Enter’key.
The display now shows ‘blo’ for blow-out.
d. Preparing for pipetting: Press the pipetting key
once to move the piston to its start position.
The arrow in the display points upwards
(aspiration).
e. Aspirate liquid: Press the pipetting key once
to aspirate the liquid.
f. Dispense liquid: The liquid is dispensed by
pressing the pipetting key once.
The arrow in the display points downwards
(dispensing).
g. Trigger blow-out? You do not have to do anything.
When pipetting in PIP mode, the blow-out is
performed automatically.
5.2 PIPmix mode
Program for mixing liquids. Sample is aspirated and dispensed repeatedly. Volume and speed
setting, see Set volume and Set the aspiration and dispensing speed.
a. Call up menu selection: Pressing the menu key
three times takes you to the program selection.
‘Mode’ flashes.
b. Set PIPmix mode: Use one of the arrow keys to
scroll through the modes until ‘PIPmix’ appears.
‘Mode’ continues to flash.
1x
1x
1x
1x
3x

16·ErgoOne®E Multi-Channel Pipettes · User Manual
c. ConrmPIPmixmode:Press‘Enter’key.
The display now shows ‘blo’ for blow-out.
d. Preparing for pipetting: Press the pipetting key
once to move the piston to its start position.
The arrow in the display points upwards
(aspiration).
e. Aspirate liquid: Press the pipetting key once
to aspirate the liquid.
f. Dispense liquid in PIPmix mode: Pressing and
holding the pipetting key causes the liquid to
be alternately dispensed and aspirated.
The display alternately shows the arrow
symbol for aspiration or dispensing as well as the
number of cycles.
g. End pipetting: Pressing the pipetting key once
dispenses the liquid and triggers the blow-out.
After dispensing the residual liquid (blow-out), the
display jumps back to the set mode (start position).
NOTICE!
The display shows a maximum of 19 cycles.
5.3 revPIPmode
Program especially for pipetting liquids with high viscosity, high vapor pressure, or foaming media.
Volume and speed setting, see Set volume and Set the aspiration and dispensing speed.
a. Call up menu selection: Pressing the menu key
three times takes you to the program selection.
‘Mode’ flashes.
1x
1x
1x
1x
Hold pressed
3x

ErgoOne® EMulti-ChannelPipettes·UserManual·17
English
Pipetting programs
b. set revPIP mode: Use one of the arrow keys to
scroll through the modes until ‘revPIP’ appears.
‘Mode’ continues to flash.
c. ConrmrevPIPmode:Press‘Enter’key.
The display now shows ‘blo’ for blow-out.
d. Preparing for pipetting: Press the pipetting key
once to move the piston to its start position.
The arrow in the display points upwards
(aspiration).
e. Aspirate liquid: Press the pipetting key once
to aspirate the liquid.
NOTICE!
When aspirating the liquid, slightly more volume is
aspirated than set.
f. Dispense liquid in revPIP mode: Press the pipetting
key once to dispense. On the display, the
arrow points down (dispensing). The set
volume is now dispensed and some liquid
remains in the tip.
g. Re-aspirate liquid in revPIP mode: Pressing
the pipetting key again now resumes the set
volume. (Pressing the pipetting key again
releases the volume)
h. Trigger blow-out: After the last pipetting, press
‘Enter’ key.
The display now once again shows ‘blo’ for blow-
out.
1x
1x
1x
1x
1x
1x

18·ErgoOne®E Multi-Channel Pipettes · User Manual
i. End pipetting: By pressing the pipetting key once,
the blow-out is triggered, and the residual liquid is
dispensed.
After dispensing the residual liquid (blow-out), the
display jumps back to the set mode (start position).
5.4 Electrophoresis (GEL) mode
Programforloadingelectrophoresisgels.Apreviouslydenedsamplevolumeisaspiratedata
high, variable speed and slowly released again.
Volume and speed setting, see Set volume and Set the aspiration and dispensing speed.
a. Call up menu selection: Pressing the menu key
three times takes you to the program selection.
‘Mode’ flashes.
b. Set GEL mode: Use one of the arrow keys to scroll
through the modes until ‘GEL’ appears.
‘Mode’ continues to flash.
c. ConrmGELmode:Press‘Enter’key.Thedisplay
now shows ‘blo’ for blow-out.
d. Preparing for pipetting: Press the pipetting key
once to move the piston to its start position.
The arrow in the display points upwards
(aspiration).
e. Aspirate liquid: Press the pipetting key once to
aspirate the liquid.
f. In order aspirate more liquid than set (up to max.
110% of the nominal volume), keep the pipetting
key pressed during the aspiration process until
the desired volume has been aspirated.
A rhombus appears in the display.
1x
3x
1x
1x
1x
Hold pressed

ErgoOne® EMulti-ChannelPipettes·UserManual·19
English
Pipetting programs
g. Dispense liquid in GEL mode: To dispense, briefly
press the pipetting key once. A rhombus appears
in the display. The aspirated volume is slowly
released again.
h. The dispensing of the sample can be interrupted by
pressing the pipetting key again.
The display shows the volume of the liquid
dispensed.
i. Trigger blow-out: After the last pipetting, press
‘Enter’ key.
The display now once again shows ‘blo’ for blow-
out.
j. End pipetting: By pressing the pipetting key once,
the blow-out is triggered, and the residual liquid is
dispensed.
After dispensing the residual liquid (blow-out), the
display jumps back to the set mode (start position).
NOTICE!
The GEL mode requires very slow dispensing speeds in order to prevent sample turbulence. In
order to ensure optimum dispensing, the dispensing speed is set at the factory. It is considerably
slower than the adjustable level 1 and cannot be selected individually.
5.5 DISPmode
Program for dispensing an aspirated liquid in partial steps. Slightly more fluid is aspirated than is
mathematically necessary.
Volume and speed setting, see Set volume and Set the aspiration and dispensing speed.
a. Call up menu selection: Pressing the menu key
three times takes you to the program selection.
‘Mode’ flashes.
b. Set DISP mode: Use one of the arrow keys to scroll
through the modes until ‘DISP’ appears.
‘Mode’ continues to flash.
1x
1x
1x
3x

20·ErgoOne®E Multi-Channel Pipettes · User Manual
c. ConrmDISPmode:Press‘Enter’key.
The display now shows ‘blo’ for blow-out.
d. Preparing for pipetting: Press the pipetting key
once to move the piston to its start position.
The arrow in the display points upwards
(aspiration).
e. Setpartialvolume:Pressingthearrowkey(+\−)
sets the volume. Pressing and holding the arrow
key causes the volume to change quickly.
‘VOL’ flashes.
f. Conrmpartialvolume:Press‘Enter’key.The
display shows the newly set partial volume.
‘Steps’ flashes. The maximum possible number of
steps is displayed.
g. Set the number of steps: Pressing the arrow key
(+/-) sets the number of steps.
‘Steps’ continues to flash.
h. Conrmnumberofsteps:Press‘Enter’key.
The display shows the set number of steps.
i. Aspirate liquid: Press the pipetting key once
to aspirate the liquid.
j. Dispense liquid: Each time the pipetting key is
pressed, a dispensing step is performed. The
arrow in the display points downwards
(dispensing). The ‘Step’ display shows the
number of steps remaining.
1x
1x
1x
1x
1x