Waters Prep 150 LC System User manual

Prep 150 LC System
Quick Start
715003940 / Revision B
Copyright © Waters Corporation 2014
All rights reserved


ii August 8, 2014, 715003940 Rev. B

General Information
Copyright notice
© 2014 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF
AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT
OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT
THE WRITTEN PERMISSION OF THE PUBLISHER.
The information in this document is subject to change without notice and should
not be construed as a commitment by Waters Corporation. Waters Corporation
assumes no responsibility for any errors that may appear in this document. This
document is believed to be complete and accurate at the time of publication. In no
event shall Waters Corporation be liable for incidental or consequential damages
in connection with, or arising from, its use. For the most recent revision of this
document, consult the Waters Web site (waters.com).
Trademarks
Waters and “THE SCIENCE OF WHAT’S POSSIBLE.” are registered trademarks
of Waters Corporation, and ChromScope is a trademark of Waters Corporation.
Other registered trademarks or trademarks are the sole property of their owners.
Customer comments
Waters’ Technical Communications organization invites you to report any errors
that you encounter in this document or to suggest ideas for otherwise improving
it. Help us better understand what you expect from our documentation so that we
can continuously improve its accuracy and usability.
We seriously consider every customer comment we receive. You can reach us at
[e-mail].
Contacting Waters
Contact Waters® with enhancement requests or technical questions regarding the
use, transportation, removal, or disposal of any Waters product. You can reach us
via the Internet, telephone, or conventional mail.
August 8, 2014, 715003940 Rev. B iii

Waters contact information:
Internet The Waters Web site includes contact information for Waters loca-
tions worldwide.
Visit www.waters.com
Telephone
and fax
From the USA or Canada, phone 800 252-HPLC, or fax 508 872
1990.
For other locations worldwide, phone and fax numbers appear in
the Waters Web site.
Conventional
mail
Waters Corporation
34 Maple Street
Milford, MA 01757
USA
Safety considerations
Some reagents and samples used with Waters instruments and devices can pose
chemical, biological, or radiological hazards (or any combination thereof). You
must know the potentially hazardous effects of all substances you work with.
Always follow Good Laboratory Practice, and consult your organization’s safety
representative for guidance.
FCC radiation emissions notice
Changes or modifications not expressly approved by the party responsible for
compliance, could void the users authority to operate the equipment. This device
complies with Part 15 of the FCC Rules. Operation is subject to the following two
conditions: (1) this device may not cause harmful interference, and (2) this device
must accept any interference received, including interference that may cause
undesired operation.
Canada spectrum management emissions notice
This class A digital product apparatus complies with Canadian ICES-003.
Cet appareil numérique de la classe A est conforme à la norme NMB-003.
Electrical power safety notice
Do not position the instrument so that it is difficult to operate the disconnecting
device.
iv August 8, 2014, 715003940 Rev. B

Safety hazard symbol notice
Documentation needs to be consulted in all cases where the symbol is used to
find out the nature of the potential hazard and any actions which have to be
taken.
Equipment misuse notice
If the equipment is used in a manner not specified by the manufacturer, the protec-
tion provided by the equipment may be impaired.
Safety advisories
Consult Appendix A for a comprehensive list of warning and caution advisories.
Operating the Prep 150 LC system
When operating the Prep 150 LC system, follow standard quality-control (QC)
procedures and the guidelines presented in this section.
Applicable symbols
Symbol Definition
Manufacturer
Date of manufacture
Authorized representative of the European Community
Confirms that a manufactured product complies with all applic-
able European Community directives
or
Australia EMC compliant
Confirms that a manufactured product complies with all applic-
able United States and Canadian safety requirements
Consult instructions for use
August 8, 2014, 715003940 Rev. B v

Symbol Definition
Supply ratings
Electrical and electronic equipment with this symbol may
contain hazardous substances and should not be disposed of as
general waste.
For compliance with the Waste Electrical and Electronic Equip-
ment Directive (WEEE) 2012/19/EU, contact Waters Corpor-
ation for the correct disposal and recycling instructions.
Safety information
Consult the operator's guides of the instruments or devices associated with this
system for information about how to safely operate and maintain them.
ISM classification
ISM Classification: ISM Group 1 Class B
This classification has been assigned in accordance with IEC CISPR 11 Industrial
Scientific and Medical (ISM) instruments requirements. Group 1 products apply to
intentionally generated and/or used conductively coupled radio-frequency energy
that is necessary for the internal functioning of the equipment. Class B products
are suitable for use in both commercial and residential locations and can be
directly connected to a low voltage, power-supply network.
EC authorized representative


 
Waters Corporation
Stamford Avenue
Altrincham Road
Wilmslow SK9 4AX UK

 
Telephone:
Fax:
Contact
[phone]
[phone]
Quality manager
vi August 8, 2014, 715003940 Rev. B

Preface
This guide is part of the documentation set for the Waters Prep 150 LC system. It
provides you with basic information about the major tasks you can perform with
the Prep 150 LC system. For detailed information, refer to the Prep 150 LC
system's Online Information System that you can access by clicking Help in the
ChromScope software.
Audience and purpose
Waters designed the Prep 150 LC system for "walk-up" use by chemists who must
meet purification requirements. The system is particularly effective where rapid
preparative separations of complex mixtures are required to establish initial work-
flow criteria. It is not intended for use in diagnostic or biologically hazardous
applications.
Use the system to perform various sampling tasks in these environments:
lPharmaceutical development
lQuality assurance and quality control
lChemical materials
lEnvironmental sciences
lAcademic laboratories
Documentation conventions
Instructions in this documentation adhere to the following conventions.
>Denotes the path for selecting menu commands, for moving from one
menu to another, or for performing a task.
Example: Click File > Open
< > Indicates that you must enter variable information, such as a path or
filename.
Example: Type open <filename>
August 8, 2014, 715003940 Rev. B vii

viii August 8, 2014, 715003940 Rev. B
Preface

Prep 150 LC System
This quick-start guide presents nine elementary procedures that provide a basic
knowledge of the Prep 150 LC system's operation and capabilities. Though relat-
ively broad in scope, the guide offers enough instruction to allow purification of
product compounds and collection of fractions. With the information in this guide,
you can experiment with the system, learning for yourself minor details and vari-
ations on the prescribed practices. For more detailed information about each of the
procedures, open the online Help window in the ChromScope software, and browse
the list of topics there.
The nine procedures appear as individual chapters:
1Start the system
2 Set up your work environment
3 Verify the system and module settings
4 Select and run a method-screening sample list
5 Create and load the run's methods and sample list
6 Start and monitor the run
7 Analyze the run's data
8 Report the results
9 Prepare the system for the next run or for shutting down
August 8, 2014, 715003940 Rev. B 9

Start the system
To start the Prep 150 LC system and prepare it for operation, follow this proced-
ure in conjunction with Good Laboratory Practice (GLP) guidelines and your labor-
atory's standard operating procedures.
Starting sequence:
To start the system and prepare it for operation:
1. Power-on the individual system modules by actuating the power switch on
each system module.
Important: If you are using the optional Prep Degasser with the 2545 QGM,
ensure the degasser is operating.
2. Power-on the ChromScope PC workstation.
Requirement: You must wait at least 30 seconds after powering-on the last
module before you start the ChromScope software and Waters Instrument
Console. Doing so is necessary for the components to communicate properly
with the software during startup.
3. From the Windows desktop, double-click the ChromScope shortcut .
Alternative: From the Windows Start menu, click All Programs, and click
ChromScope.
10 August 8, 2014, 715003940 Rev. B
Prep 150 LC System

Requirement: Unless the system administrator has disabled the System
Secure function, you must be a registered user and provide your user name
and password, to start and log on to the ChromScope software.
4. Enter your user name and password, and then click Login, to start the
ChromScope software.
5. On the System Configuration Detector tab, click Lamp On.
Requirement: Allow the lamp to warm for a minimum of 30 minutes before
performing an injection, to stabilize its optics.
6. Inspect the column's tubing connections to ensure that the column or unions
are connected, leaving no open flows.
7. Inspect the gradient pump's solvent bottles for mobile phase and wash flows,
to ensure the volumes are adequate for your methods.
Preparing the solvent pump
When the solvent lines are dry, including for initial startup, or when changing
solvents, prime the pump's solvent and seal-wash flow paths, to remove any air
bubbles in the flow paths between each solvent bottle and pump. To prime the 2545
QGMor 2545 BGM, double-click the solvent pump in the ChromScope system
panel, and then refer to priming instructions in the 2535/2545/2555 Quaternary
Gradient Module Operator's Guide.
Preparing the sample injector
You must prepare the sample injector according to its type, manual or 2707 Auto-
sampler.
To prepare the manual injector:
1. With the column offline, flush the injector and sample loop with a suitable
solvent, to remove any trapped air or contaminants from the injection flow
path.
2. Prepare your sample for loading by inserting a syringe into the injector port.
To prepare the 2707 Autosampler:
1. Load samples, and ensure the wash-solvent bottle is full.
2. When the solvent line is dry, including for initial startup, or after changing
solvents or samples, on the System Configuration Autosampler tab, click
Needle Wash, to clean and purge any air bubbles from the syringe and buffer
tubing.
Tip: For best results, repeat the needle wash routine 2 or 3 times.
Preparing the fraction bed and waste container
You must prepare the WFC III module to collect fractions and waste during a puri-
fication run.
August 8, 2014, 715003940 Rev. B 11
Start the system

To prepare for fraction and waste collection:
1. Inspect the fraction collection tubes and collection bed layout, to verify the
module is ready to receive the run's fractions and waste.
2. Inspect the waste container, to ensure it can collect waste.
See also: Waters Fraction Collector III Operator's Guide.
Verifying operational readiness
Optionally, run an instrument method using the Monitor control, to set initial
conditions and verify the system's operational readiness.
Tip: You can view real-time status and plots in the ChromScope System Panel and
Current Run tabs, to monitor flow and detector baselines.
For more information, see these topics in the ChromScope online Help:
lConfigure system and module settings
lDetermine system status
lMaintain the 2545 Binary Gradient Module
lCreate methods for controlling system functions
12 August 8, 2014, 715003940 Rev. B
Prep 150 LC System

Set up your work environment
Before you begin using ChromScope software for the Prep 150 LC system to purify
product and collect fractions, you can customize the system according to your pref-
erences. These customizations become part of your user profile. As such, they
appear whenever you log on to the system.
August 8, 2014, 715003940 Rev. B 13
Set up your work environment

Task Description
Set up your project
folder.
You can create a new project or work with existing projects.
When you create a new project in the system, a project folder
bearing the project name appears under the project's root
node. Inside the project folder, the ChromScope software
creates a subfolder structure to organize each file type that
exists in the project.
Alternative: Use the default project to get started, and then
add new projects later to organize your files.
To set up a new project:
1. Right-click the Project Folders node, and select Add New
Project.
Alternative: In the toolbar, click Add Project .
2. In the Configure Project dialog box, enter the project's
name and file location.
3. Select a subfolder naming convention from the list for
organizing the project's data-log files.
Restriction: After you create and save your new project,
you cannot change its subfolder naming convention.
4. To import the files from another project into your new
project, select the project from the list of available
projects, and then select the check boxes for the types of
files that you want to import.
Tip: Select "Select All" to import all of the project's files.
5. Click OK, to save your settings and create the new
project.
Result: The new project appears in the navigation tree,
under the Projects node.
14 August 8, 2014, 715003940 Rev. B
Prep 150 LC System

Task Description
Decide how you
want to open and
work with the
acquisition data-log
files.
You can select from among three file-open modes (New Tab,
Current Tab, or Current Tab Overlay) according to how you
want to open and view data-log files. When you select a
mode, all data-log files you open remain open in that mode
until you select another mode.
Alternative: Accept the default file-open mode, New Tab, to
open each data-log file in a new tab in the ChromScope main
window.
The table below describes each of the three modes and its
effect on currently opened data-log files when you use it to
open a new data file.
File-
open
mode
File tab caption
upon opening a
new data-log file
Effect on currently
opened data-log files
New
Tab
A new file (outer)
tab, which
displays the file's
name, is created
for each data-log
file that you open.
All opened data-log
files remain open.
Current
Tab
The data-log file is
opened in the
currently selected
tab, which
displays the file's
name.
The data-log file that
was previously opened
in the current tab is
closed. Other data-log
files opened in other
tabs remain open.
Current
Tab
Overlay
The data-log file is
opened in the
currently selected
tab, which
displays the file's
name.
All opened data-log
files in the current tab
remain open. The tab
displays the name of
the last opened file.
To set the file-open mode:
From the File menu, click File Open Mode > New Tab,
Current Tab, or Current Tab Overlay.
Alternative: From the toolbar, click New Tab , Current
Tab , or Current Tab Overlay .
Rules:
lFor New Tab and Current Tab, the information in the
data grid pertains only to the single, opened data-log file.
August 8, 2014, 715003940 Rev. B 15
Set up your work environment

Task Description
lFor Current Tab Overlay, the information in the data
grid pertains to all of the data-log files opened in the
current tab.
Customize the
display of plots and
run data.
In the Display Options window, you can specify and save
your preferences for viewing chromatograms and their asso-
ciated data in the data-log file's data grid. Use these pref-
erences to show or hide various elements of the data-log file's
raw data, and to set preferences for how you want to display
pressure units.
Alternative: You can accept the default settings. Later, as
you gain familiarity with the system, you can change any of
the settings in the Display Options panel, to accommodate
your preferences.
To set display options:
1. From the Edit menu, click Display Options.
Alternative: In the toolbar, click Display Options.
2. In the Display Options window, edit the settings accord-
ing to your preferences.
3. Click OK, to save your display settings.
16 August 8, 2014, 715003940 Rev. B
Prep 150 LC System

Task Description
Decide how to
configure and send
reports.
You can configure and generate individual custom reports
using a predefined format, or set up batch processing to
process a group of data-log files sequentially. For batch
processing, you can also configure an option to automatically
distribute reports to a list of email recipients as a function
of a sample list's batch "run and report" queue. The reports
are based on the integration-method and report-template
settings in the sample list. For details, see the "Managing
Data and Reporting" section in the ChromScope online Help.
Alternative: After your run, open the data-log file, and from
the File menu, click "Print Quick Report to PDF" or "Print
Quick Report to Printer", to generate a predefined report of
your data.
Import data from
other Waters
products.
You can import data-log files (*.tta) from a defined location
into a project's Data Files folder (or subfolder). This oper-
ation is useful for importing scouting-run information used
to set up the timing parameters for stacked-injection sample
lists.
Alternative: Use the system to generate your own scouting-
run data, or manually define your injection and collection
timing in the run's sample list and collection method.
To import data-log files:
1. In the navigation tree, right-click on the destination pro-
ject's Data Files node (or its subfolder), and select Import
Data Files.
2. Select the data-log files that you want to import from the
list, and click Open.
Result: The data-log files appear in the Project's Data
Files node (or its subfolder).
For more information, see these topics in the ChromScope online Help:
lWork with projects
lWork with views
lConfigure your display preferences
lGenerate and print a custom report
lImport and export data-log files
August 8, 2014, 715003940 Rev. B 17
Set up your work environment

Verify the system and module settings
nNavigation tree > System Configuration > System Settings, Pump, Solvent Selector, Autosampler, and
Detector tabs
nNavigation tree > System Status > Collection Bed Status
To manage the system's operational readiness, you must define your preferences
for shutting down the system in response to certain operating conditions, and for
configuring the individual module's alert thresholds and usage settings. You must
also view a module's real-time device status for maintenance or diagnostic
purposes. You perform these actions using the tabs under the System Config-
uration and System Status nodes in the ChromScope software.
Alternative: To begin when using a standard Prep 150 LC manual or automated
system configuration, accept the software's default system configuration settings.
Later, as you gain familiarity with the system, you can change any of the settings
in the System Configuration tabs to accommodate your system modifications or
preferences.
Requirement: For fraction collection, before starting a purification run, you must
ensure that the collection bed and tube settings in the ChromScope Collection Bed
Status tab correlate to the bed layout and tube locations in the WFC III module's
collection bed.
18 August 8, 2014, 715003940 Rev. B
Prep 150 LC System

To verify the system and module settings required for your configuration:
1. In the navigation tree, click the System Configuration node.
2. Click the tab for the system or module settings that you want to verify.
Tip: To set up your solvent levels and warning and alarm thresholds, click
the Solvent Selector tab, and configure the settings for each of your solvents.
3. Use the default values, or make any changes you require, and click Apply to
save your settings.
4. In the navigation tree, click the System Status node.
5. Click the Collection Bed Status tab, to configure the collection bed layout
and tube settings for the WFC III module's collection bed.
For more information, see these topics in the ChromScope online Help:
lConfigure system and module settings
lSet up the collection bed in ChromScope
August 8, 2014, 715003940 Rev. B 19
Verify the system and module settings

Select and run a method-screening sample list
To optimize collection of purified product, you must fine tune the purification chro-
matography on the system using a scouting run, and store the optimal settings in
a data-log file. To do so, you can select and run a method-screening sample list.
Later, you must build a purification sample list, using the integrated data from
plots in the scouting run's data-log file, to calculate and specify fraction-collection
and injection-timing settings. This technique is especially useful when you are
working with unknown samples or using the Sample List wizard to build stacked-
injection sample lists for high-throughput purification.
Note: If the scouting-run data are already on the system's ChromScope work-
station, or if you are working with known samples and do not need to fine tune the
chromatography beforehand, skip this step.
To select and run a method-screening sample list:
1. Select a method-screening sample list, or create one using the Sample List
wizard, to help you fine tune the parameter settings used to isolate your
sample compounds.
20 August 8, 2014, 715003940 Rev. B
Prep 150 LC System