Waters 2410 User manual

Waters
Refractometer
Service Manual
Waters Corporation
Service Communications
34 Maple Street
Milford, MA 01757
Manual Number 071604
Rev. 4, May 1998

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REFRACTOMETER SERVICE MANUAL
TABLE OF CONTENTS
CHAPTER 1 - INTRODUCTION
Refractive Index Detectors ......................................................................................................................1-1
Performance Specifications of LC Detectors............................................................................................1-2
Comparative Refractometer Specifications ..............................................................................................1-4
Solvent Degassing for Liquid Chromatography........................................................................................l-6
CHAPTER 2 - M410 GENERAL INFORMATION
M410 Features........................................................................................................................................2-1
CHAPTER 3 - M410 START UP
Pre Start Up Requirements......................................................................................................................3-1
Start Up Kit ............................................................................................................................................3-1
Fluid Connections ..................................................................................................................................3-2
Interfacing Connections ..........................................................................................................................3-4
Start Up Check Sheet .............................................................................................................................3-6
Choosing Sensitivity and Scale Factor Values .......................................................................................3-11
CHAPTER 4 - M410 FRONT AND REAR PANEL
Front Panel General Information..............................................................................................................4-l
Front Panel Control ................................................................................................................................4-4
Front Panel Assembly.............................................................................................................................4-8
Front Panel Schematics...........................................................................................................................4-9
Rear Panel Connections ........................................................................................................................4-10
Rear Panel Assembly.............................................................................................................................4-13
Rear Panel.............................................................................................................................................4-14
Rear Panel - Oven Compartment...........................................................................................................4-15
Refractometer Power Wiring.................................................................................................................4-16
CHAPTER 5 - M410 OPTICS ASSEMBLY
Optics Assembly Location.......................................................................................................................5-1
Optics Assembly General Description .....................................................................................................5-2
LED Light Source...................................................................................................................................5-3
Photodetector Assembly..........................................................................................................................5-4
Flow Cell End Cap Assembly..................................................................................................................5-5
Counter Current Heat Exchanger ............................................................................................................5-6
CHAPTER 6 - M410 CONTROL BOARD
Control Board Functional Description.....................................................................................................6-1
Control Board Assembly Drawing...........................................................................................................6-3
Control Board Potentiometer Adjustments...............................................................................................6-4
Control Board Test Points.......................................................................................................................6-5
Control Board Assembly Drawing...........................................................................................................6-6
Control Board Schematics.....................................................................................................................6-13

TABLE OF CONTENTS cont.
CHAPTER 7 - M410 ANALOG BOARD
Analog Board Functional Description .....................................................................................................7-1
Amplifier Circuit Block Diagram............................................................................................................7-2
Analog Board Block Diagram.................................................................................................................7-3
Analog Board Potentiometer Adjustments...............................................................................................7-4
Analog Board Assembly Drawing...........................................................................................................7-6
Analog Board Schematics.......................................................................................................................7-8
CHAPTER 8 - M410 SERVICE PROCEDURES
M410 General Troub1eshooting..............................................................................................................8-1
Troubleshooting Guide - Electronic Problems.........................................................................................8-4
Diagnostics............................................................................................................................................. 8-9
Optics Bench Assembly Replacement Procedure ................................................................................... 8-11
Flow Cell End Cap Assembly Replacement........................................................................................... 8-14
LED Assembly Replacement................................................................................................................. 8-18
Photodiode Assembly Replacement....................................................................................................... 8-20
Optical Alignment Procedure................................................................................................................ 8-22
Counter Current Heat Exchanger Replacement ..................................................................................... 8-24
RTD Sensor Replacement ..................................................................................................................... 8-26
Heater Replacement.............................................................................................................................. 8-27
Solenoid Valve Replacement................................................................................................................. 8-30
Oven Fan Replacement .........................................................................................................................8-31
Control Board Replacement .................................................................................................................. 8-33
Analog Board Replacement................................................................................................................... 8-35
M410 Test Specifications...................................................................................................................... 8-37
M410 Bandspread................................................................................................................................. 8-38
Lift Test (Sensitivity Test)..................................................................................................................... 8-39
CHAPTER 9 - M410 SPARE PARTS WARRANTY
M410 Common Replacement Parts.........................................................................................................9-1
Optics Assembly Exploded Diagram .......................................................................................................9-2
Optics Bench Assembly Drawing............................................................................................................ 9-4
Complete Parts List................................................................................................................................. 9-5
Warranty Statement.............................................................................................................................. 9-30
CHAPTER 10 - R401 GENERAL INFORMATION
R401 General Description..................................................................................................................... 10-1
R401 Functional Description................................................................................................................. 10-3
Refractometer Modes............................................................................................................................ 10-7
Refractometer Temperature Control...................................................................................................... 10-8
R401 Troubleshooting........................................................................................................................... 10-9
CHAPTER 11 - R401 START UP
Check Out Procedure ............................................................................................................................ 11-1
Fluid Connections................................................................................................................................. 11-2
Connecting the R401 to a Data Module................................................................................................. 11-3

TABLE OF CONTENTS cont.
CHAPTER 12 - R401 SERVICE PROCEDURES
Cleaning the Flow Cell and Inlet Lines .................................................................................................12-1
Flow Cell Replacement .........................................................................................................................12-2
Source Light and Photocell Replacement...............................................................................................12-2
Aligning the R401 Optics......................................................................................................................12-4
R401 Mask Adjustment.........................................................................................................................12-7
Linearity Test........................................................................................................................................12-9
Amplifier Input and Output Checks..................................................................................................... 12-10
Printed Circuit Board Assembly..........................................................................................................12-12
Wiring Schematic ...............................................................................................................................12-13
CHAPTER 13 - R401 SPARE PARTS/WARRANTY
R401 Recommended Replacement Parts................................................................................................13-1
Complete Parts List...............................................................................................................................13-3
Warranty Statement ............................................................................................................................ 13-11
CHAPTER 14 - 2410 OVERVIEW
Introduction ..........................................................................................................................................14-1
Design Features.....................................................................................................................................14-1
Serviceability Improvements .................................................................................................................14-2
CHAPTER 15 - 2410 SPECIFICATIONS
Operational Specifications.....................................................................................................................15-1
Integrator Output ..................................................................................................................................15-1
Optical Components..............................................................................................................................15-2
Environmental Specifications................................................................................................................15-2
Dimensions...........................................................................................................................................15-2
Electrical Specifications........................................................................................................................15-3
CHAPTER 16 - 2410 INSTALLATION
Installation Requirements......................................................................................................................16-1
Startup Kit Contents..............................................................................................................................16-1
Startup Procedure..................................................................................................................................16-1
CHAPTER 17 - 2410 SERVICE INFORMATION
LC Interface..........................................................................................................................................17-1
Optics ...................................................................................................................................................17-2
Electronics............................................................................................................................................17-3
Power Distribution Board......................................................................................................................17-4
15 Volt Output.................................................................................................................................17-4
100 Volt Output...............................................................................................................................17-4
Heater Control.................................................................................................................................17-4
Static and EMI Precautions..............................................................................................................17-4
Test Points and LEDs ......................................................................................................................17-4
Low Voltage Power Supply ...................................................................................................................17-5
CPU Board............................................................................................................................................17-7
Temperature Channel ......................................................................................................................17-8

TABLE OF CONTENTS cont.
Fan Fail Circuit ............................................................................................................................... 17-8
Event Inputs .................................................................................................................................... 17-8
Purge and Heater Controls............................................................................................................... 17-8
Power Distribution and Circuitry..................................................................................................... 17-9
DAC Output Circuitry ..................................................................................................................... 17-9
Diagnostics......................................................................................................................................... 17-10
CHAPTER 18 - 2410 SPARE PARTS
Electrical Spares................................................................................................................................... 18-1
Cable Spares......................................................................................................................................... 18-1
Mechanical Spares................................................................................................................................ 18-1
Optical Spares....................................................................................................................................... 18-1
Miscellaneous Spares............................................................................................................................ 18-2
CHAPTER 19 - 2410 WARRANTY
Express Warranty................................................................................................................................. 19-1
Limited Warranty............................................................................................................................ 19-1
Exclusions....................................................................................................................................... 19-1
Exclusive Remedy............................................................................................................................ 19-1
Disclaimers...................................................................................................................................... 19-1
Warranty Service............................................................................................................................. 19-2
Warranty Service Exceptions........................................................................................................... 19-2
Warranty Period .............................................................................................................................. 19-3
Shipments, Damages, Claims and Returns............................................................................................ 19-4

Chapter 14 - 2410 Overview
Introduction..............................................................................................................14-1
Design Features.......................................................................................................14-1
Serviceability Improvements...................................................................................14-2

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14-1
Introduction
Figure 1 - 2410 Detector
The 2410 detector is functionally equivalent to its predecessor, the 410 detector. Significant changes have
been made to update the 410 to the new 2410 detector design platform, including improvements for
serviceability.
Design Features
The form factor of the 2410 is the same as the 2487.
A single CPU board now performs the functions previously performed by the 410 analog and control boards.
The transformer has been eliminated and replaced with the same switching power supply used in the 2487.
Waters part number WAS081226 can be used to order power supplies for use in either the 2487 or 2410
detectors.
Maximum output from the analog outputs is –2.0 to +2.0 volts. Both Recorder Out and Integrate Out are
attenuated outputs. In the 410 detector, a sensitivity setting of 2 or greater could generate as much as 16v of
output signal.
The optics bench is the same bench used in the 410 detector.

14-2
Serviceability Improvements
An optics access door has been incorporated to facilitate the replacement of the heater fan, heater, RTD,
photodiode, LED and APB signal connectors.
New diagnostics are easier to use and can diagnose problems more accurately.
•The IEEE address is set in diagnostics.
•Integrator offsets from 0 to 50 mV.
•A 2487 style analog noise and drift test is built into diagnostics.
Each valve is a self-contained unit. Valves snap in and out for replacement without the use of tools.
The 2410 design incorporates quick disconnects instead of screws. This change makes service of the detector
simpler and faster.
•The optics bench cover, optics bench, oven fan, RTD, and heater assembly are mounted with quick
disconnect snap connectors.
•The pressure relief and purge valves are mounted in slip clamps.
•The two bulk head fittings have been replaced by a single tee.

Chapter 15 - 2410 Specifications
Operational...............................................................................................................15-1
Integrator Output......................................................................................................15-1
Optical Components ...............................................................................................15-2
Environmental ..........................................................................................................15-2
Dimensions...............................................................................................................15-2
Electrical...................................................................................................................15-3

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15-1
Operational Specifications
Conditions Specifications
RI Range 1.00 to 1.75 RIU
Measurement Range 5 x 10-3 RIUmaximumFS(SENS=1,SF=1)
5x10
-3 minimum FS (SENS = 1024, SF = 100)
Flow Rate 0.03 to 20 mL/min
Noise* 2.0x10
-8 RIU, (TC = 1, SF = 80, SENS = 64, THF at
24 °C with restrictor coil)
Drift * 2.5 × 10–7 RIU/hr (static with THF) TC = 1, SF = 20,
SENS = 32
Sensitivity Settings 1, 2, 4, 8, 16, 32, 64, 128, 256, 512, 1024
Time Constant Filter
Settings 0.2, 1, 3, 10 seconds
Analog Outputs –2.0 V to +2.0 V
Temperature Control Internal oven: 30 to 50 °C (86 to 122 °F)
External column heaters:
•Ambient to 150 °C (302 °F), steel
•Ambient to 80 °C (176 °F), plastic
* with one hour warm-up time
Integrator Output
Sensitivity Setting Output (V)
11
22
42
82
16 to 1024 2

15-2
Optical Components
Conditions Specifications
Light Source Pulsed light emitting diode (LED)
Flow Cell Fused quartz
Cell Volume 10 µL
Pressure Limit 100 psi
Fluidic Path Materials 316 stainless steel, Dynasil®, Suprasil II®, PTFE,
Kalrez®, quartz
Environmental Specifications
Conditions Specifications
Operating Temperature 15 °C to 32.2 °C (59 °F to 90 °F)
Operating Humidity 20 to 80%, noncondensing
Shipping and Storage Temperature –40 °C to 70 °C (–104 °F to 158 °F)
Shipping and Storage Humidity 0% to 90%, noncondensing
Dimensions
Conditions Specifications
Height 8.2 in. (20.8 cm)
Length 19.8 in. (50.3 cm)
Width 11.2 in. (28.4 cm)
Weight 21.7 lbs. (9.7 kg)