Waters ACQUITY Isocratic Solvent Manager Operating manual

ACQUITY Isocratic
Solvent Manager
Overview and Maintenance Guide
715004208 / Revision A
Copyright © Waters Corporation 2013
All rights reserved

ii September 26, 2013, 715004208 Rev. A

September 26, 2013, 715004208 Rev. A iii
General Information
Copyright notice
© 2013 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF
AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS
DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY
FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.
The information in this document is subject to change without notice and
should not be construed as a commitment by Waters Corporation. Waters
Corporation assumes no responsibility for any errors that may appear in this
document. This document is believed to be complete and accurate at the time
of publication. In no event shall Waters Corporation be liable for incidental or
consequential damages in connection with, or arising from, its use. For the
most recent revision of this document, consult the Waters Web site
(waters.com).
Trademarks
ACQUITY, ACQUITY UPLC, Alliance, “THE SCIENCE OF WHAT’S
POSSIBLE.”, UPC2, UPLC, and Waters are registered trademarks of Waters
Corporation.
Nitronic is a registered trademark of AK Steel.
Pharmed is a registered trademark of Saint-Gobain Performance Plastics
Corporation.
PEEK is a trademark of Victrix PLC.
TORX is a registered trademark of Textron, Inc.
Other registered trademarks or trademarks are the sole property of their
owners.
Customer comments
Waters’ Technical Communications organization invites you to report any
errors that you encounter in this document or to suggest ideas for otherwise
improving it. Help us better understand what you expect from our

iv September 26, 2013, 715004208 Rev. A
documentation so that we can continuously improve its accuracy and
usability.
We seriously consider every customer comment we receive. You can reach us
at [email protected].
Contacting Waters
Contact Waters®with enhancement requests or technical questions regarding
the use, transportation, removal, or disposal of any Waters product. You can
reach us via the Internet, telephone, or conventional mail.
Safety considerations
Some reagents and samples used with Waters instruments and devices can
pose chemical, biological, or radiological hazards (or any combination thereof).
You must know the potentially hazardous effects of all substances you work
with. Always follow Good Laboratory Practice, and consult your organization’s
safety representative for guidance.
Waters contact information:
Contacting medium Information
Internet The Waters Web site includes contact
information for Waters locations worldwide.
Visit www.waters.com.
Telephone and fax From the USA or Canada, phone 800
252-4752, or fax [phone].
For other locations worldwide, phone and fax
numbers appear in the Waters Web site.
Conventional mail Waters Corporation
34 Maple Street
Milford, MA 01757
USA

September 26, 2013, 715004208 Rev. A v
Considerations specific to the ISM
High voltage hazard
FCC radiation emissions notice
Changes or modifications not expressly approved by the party responsible for
compliance, could void the users authority to operate the equipment. This
device complies with Part 15 of the FCC Rules. Operation is subject to the
following two conditions: (1) this device may not cause harmful interference,
and (2) this device must accept any interference received, including
interference that may cause undesired operation.
Canada spectrum management emissions notice
This class A digital product apparatus complies with Canadian ICES-003.
Cet appareil numérique de la classe A est conforme à la norme NMB-003.
Electrical power safety notice
Do not position the instrument so that it is difficult to operate the
disconnecting device.
Safety hazard symbol notice
Documentation needs to be consulted in all cases where the symbol is
used to find out the nature of the potential hazard and any actions which have
to be taken.
Equipment misuse notice
If the equipment is used in a manner not specified by the manufacturer, the
protection provided by the equipment may be impaired.
Warning: To avoid electric shock, do not remove the device’s protective
panels. The components within are not user-serviceable.

vi September 26, 2013, 715004208 Rev. A
Safety advisories
Consult Appendix A for a comprehensive list of warning and caution
advisories.
Operating this device
When operating this device, follow standard quality-control (QC) procedures
and the guidelines presented in this section.
Applicable symbols
Symbol Definition
Manufacturer
Authorized representative of the European
Community
Confirms that a manufactured product complies
with all applicable European Community
directives
Australia C-Tick EMC compliant
Confirms that a manufactured product complies
with all applicable United States and Canadian
safety requirements
Consult instructions for use
Electrical and electronic equipment with this
symbol may contain hazardous substances and
should not be disposed of as general waste.
For compliance with the Waste Electrical and
Electronic Equipment Directive (WEEE)
2012/19/EU, contact Waters Corporation for the
correct disposal and recycling instructions.

September 26, 2013, 715004208 Rev. A vii
Audience and purpose
This guide is intended for personnel who install, operate and maintain the
ACQUITY isocratic solvent manager. It gives an overview of the technology
and operation of the isocratic solvent manager.
Intended use of the isocratic solvent manager
Waters designed the isocratic solvent manager for use in liquid
chromatography applications. The isocratic solvent manager is not intended
for use in diagnostic applications.
Industrial scientific and medical classification
Industrial scientific and medical classification: Industrial
Scientific and Medical Group 1 Class B
This classification has been assigned in accordance with IEC CISPR 11
Industrial scientific and medical instruments requirements. Group 1 products
apply to intentionally generated and/or used conductively coupled
radio-frequency energy that is necessary for the internal functioning of the
equipment. Class B products are suitable for use in both commercial and
residential locations and can be directly connected to a low voltage,
power-supply network.

viii September 26, 2013, 715004208 Rev. A
EC authorized representative
Waters Corporation (Micromass UK Ltd.)
Floats Road
Wythenshawe
Manchester M23 9LZ
United Kingdom
Telephone: [phone]
Fax: [phone]
Contact: Quality manager

September 26, 2013, 715004208 Rev. A ix
General Information .................................................................................... iii
Copyright notice .................................................................................................. iii
Trademarks ........................................................................................................... iii
Customer comments ............................................................................................ iii
Contacting Waters ............................................................................................... iv
Safety considerations .......................................................................................... iv
Considerations specific to the ISM ..................................................................... v
FCC radiation emissions notice .......................................................................... v
Canada spectrum management emissions notice .............................................. v
Electrical power safety notice ............................................................................. v
Safety hazard symbol notice................................................................................ v
Equipment misuse notice .................................................................................... v
Safety advisories................................................................................................. vi
Operating this device .......................................................................................... vi
Applicable symbols ............................................................................................. vi
Audience and purpose....................................................................................... vii
Intended use of the isocratic solvent manager................................................ vii
Industrial scientific and medical classification .......................................... vii
Industrial scientific and medical classification: Industrial Scientific
and Medical Group 1 Class B.................................................................... vii
EC authorized representative ........................................................................ viii
1 ACQUITY Isocratic Solvent Manager ................................................. 13
Location of the ISM in a system ..................................................................... 14
Major components ............................................................................................. 15
Optional ISM kits .............................................................................................. 17
Flow path through the ISM with an installed Flow-splitter or
Post-column Addition kit ......................................................................... 22
Table of Contents

x September 26, 2013, 715004208 Rev. A
2 Preparing for Operation ....................................................................... 27
Stacking system modules ................................................................................. 28
Installation recommendations for fittings ................................................... 29
Installing the optional leak sensor ................................................................ 33
Resolving leak sensor errors ........................................................................... 35
Installing the waste tubing and vent tubing for the degasser ................ 40
Installing the optional ACQUITY UPLC or Alliance
Flow-splitter kit ......................................................................................... 42
Changing the split ratio .................................................................................... 48
Installing the optional ACQUITY UPC2Flow-splitter kits ...................... 49
Installing the optional ACQUITY UPC2Dual-Detection
Flow-splitter kit .......................................................................................... 49
Installing the optional ACQUITY UPC2Triple-Detection
Flow-splitter kit .......................................................................................... 56
Installing the optional Post-column Addition kit ...................................... 61
Connecting to the electricity source ............................................................. 64
Priming the seal-wash system ........................................................................ 64
Priming the ISM ................................................................................................. 67
Priming a dry ISM via the console.................................................................... 67
Priming a dry ISM using a syringe................................................................... 68
Monitoring LEDs ................................................................................................ 70
Power LED ......................................................................................................... 70
Status LED......................................................................................................... 70
ISM control panel ............................................................................................... 71
3 Maintenance Procedures ...................................................................... 75
Maintaining the ISM ......................................................................................... 76
Contacting Waters technical service................................................................. 76
Maintenance schedule ...................................................................................... 77

September 26, 2013, 715004208 Rev. A xi
Spare parts .......................................................................................................... 79
Safety and handling .......................................................................................... 79
Replacing the seal wash solvent filter .......................................................... 80
Replacing the ISM solvent filter ..................................................................... 81
Cleaning the air filter in the door .................................................................. 83
Replacing the air filter in the door ................................................................ 83
Replacing the optional leak sensor ............................................................... 84
Replacing a restrictor module in the optional ACQUITY UPLC
or Alliance Flow-splitter kit .................................................................... 87
Replacing the restrictor module in the optional ACQUITY UPC2
Flow-splitter kit .......................................................................................... 88
Replacing the inline 22-µL filter ..................................................................... 90
Replacing the low-pressure filter cartridge ................................................ 92
Replacing the vent-valve cartridge ............................................................... 96
Replacing the cartridge in the optional solvent-selection valve ............ 99
Replacing the primary check valve ............................................................. 101
Replacing the accumulator check valve ..................................................... 104
Removing the primary head and replacing its seals ............................... 107
Removing the accumulator head and replacing its seals ....................... 123
Cleaning the instrument’s exterior ............................................................. 140
A Safety Advisories .................................................................................. 141
Warning symbols .............................................................................................. 142
Specific warnings ............................................................................................. 143
Caution advisory .............................................................................................. 144
Warnings that apply to all Waters instruments and devices ................. 145
Electrical and handling symbols .................................................................. 150

xii September 26, 2013, 715004208 Rev. A
Electrical symbols ............................................................................................ 150
Handling symbols ............................................................................................ 151
B Specifications ........................................................................................ 153
Physical specifications ................................................................................... 153
Environmental specifications ....................................................................... 154
Electrical specifications ................................................................................. 154
Input/output specifications ........................................................................... 155
Performance specifications ........................................................................... 155
ISM ................................................................................................................... 155
ISM flow-splitter kits....................................................................................... 157
Wetted materials of construction ................................................................. 158
ISM ................................................................................................................... 158
ACQUITY UPLC Flow-splitter kit.................................................................. 159
Alliance Flow-splitter kit................................................................................. 159
UPC2Flow-splitter kit..................................................................................... 159
Post-column Addition kit................................................................................. 159

September 26, 2013, 715004208 Rev. A 13
1ACQUITY Isocratic Solvent
Manager
Waters designed the ACQUITY Isocratic Solvent Manager (ISM) to
control the delivery of isocratic solvent as a make-up pump in a UPC2
system. The ISM, a single pump, operates at a maximum pressure of
103,421 kPa (1034 bar, 15,000 psi), and flow rates as high as 1 mL/min.
The pressure decreases linearly, to 62,053 kPa (621 bar, 9000 psi), at
flow rates up to 2 mL/min. The ISM can also function as the primary
pump in a UPLC system.
Contents:
Topic Page
Location of the ISM in a system...................................................... 14
Major components ............................................................................ 15
Flow path through the ISM with an installed Flow-splitter or
Post-column Addition kit ........................................................... 22

1 ACQUITY Isocratic Solvent Manager
14 September 26, 2013, 715004208 Rev. A
Location of the ISM in a system
The following diagram shows the location of the ISM as a make-up pump in a
typical system that includes a QDa (Quadrupole Dalton) mass detector:
Note: The ISM is typically located in the rightmost stack in a system.
Bottle tray
PDA detector
Isocratic solvent manager
with splitter kit
QDa detector

Major components
September 26, 2013, 715004208 Rev. A 15
Major components
The following diagram shows the major components of the ISM:
ISM components:
Component Description
Accumulator pump Receives solvent from the primary pump and delivers
it to the system.
Primary
pump
Vent valve
Accumulator
pump
Drain fitting
(to waste)
Leak sensor
reservoir
Location of pressure
transducer cable
connector (2)
Seal wash
pump
Drip tray
Low-
pressure
inlet filter
Inline
22-μL filter
Accumulator
check valve
Seal-wash
waste tube
Location of leak
sensor cable
connector
On/off
switch
Power LED Run LED
Location of
solvent
selection
valve
(optional)
Location of Flow-splitter or
Post-column Addition kit
(optional)
Location of
degasser
vent tubing
Location of sample
manager purge
solvent degasser
chamber (optional)
ISM mobile phase
degasser chamber
Vent valve
waste tube
Primary
check
valve

1 ACQUITY Isocratic Solvent Manager
16 September 26, 2013, 715004208 Rev. A
Accumulator check
valve
A ball check valve that allows flow in only one
direction.
Degasser vent
tubing
Vents exhaust from the degasser pump.
Drip tray Collects and routes fluid leaks.
Drip tray drain
fitting
Conducts leakage from the drip tray and routes it to a
waste vessel.
Flow-splitter and
Post-column
Addition kits
(optional)
Several ISM kits are available for flow splitting and
post-column addition.
Inline 22-μL filter Filters the solvent before it reaches the pump outlet.
Leak sensor Continuously monitors the ISM for leaks and stops
the system flow when its optical sensor detects
approximately 1.5 mL of accumulated, leaked liquid
in its surrounding reservoir.
Note: The leak sensor is part of the system kit and
should be installed and configured in the software.
Low-pressure inlet
filter
A filter that removes particulates from the solvent.
ISM mobile phase
degasser chamber
Removes dissolved gasses from mobile phase solvent
and exhausts them, and any condensates, through the
vent valve waste tube.
Note: Vacuum degassing can change the composition
of mixed solvents.
On/off switch Powers the ISM on or off.
Power LED Indicates when the ISM is powered on or off.
Pressure
transducer cable
connectors
The electrical connections for the pressure
transducers located on the front of the actuators.
Primary check
valve
A ball check valve that allows flow in only one
direction.
ISM components: (Continued)
Component Description

Major components
September 26, 2013, 715004208 Rev. A 17
Optional ISM kits
Several optional ISM kits provide for flow splitting and post-column addition.
The flow-splitter kits allow you to adjust the amount of analytical flow diluted
and diverted to the QDa detector. Four splitter kits are available, each specific
to a Waters instrument class:
• ACQUITY®LC (UPLC®, H-Class, and I-Class) Flow-splitter kit
• Alliance®Flow-splitter kit
• ACQUITY UPC2® Dual-Detection Flow-splitter kit
• ACQUITY UPC2® Triple-Detection Flow-splitter kit
The Post-column Addition kit is used to add additional liquid to the analytical
flow stream before it enters the QDa detector. This kit is compatible with
ACQUITY UPLC and Alliance systems.
Primary pump Draws solvent, transferring it to the accumulator
pump and system as part of the serial flow design.
Run LED Indicates the ISM status.
Sample manager
purge solvent
degasser chamber
(optional)
Degasses the purge solvent which then flows through
the sample manager.
Note: Vacuum degassing can change the composition
of mixed solvents.
Seal-wash pump The pump that circulates solvent, to keep the
actuators’ high-pressure seals free of contaminants.
Seal-wash waste
tube
Conducts seal-wash waste to a waste vessel.
Solvent selection
valve (optional)
A valve that allows selection of up to six different
solvents used isocratically.
Vent valve waste
tube
Tubing that vents solvent to waste during priming.
Vent valve A valve that automatically switches to waste during
priming and a block position during the leak test.
ISM components: (Continued)
Component Description

1 ACQUITY Isocratic Solvent Manager
18 September 26, 2013, 715004208 Rev. A
Note: The Post-column Addition kit is not compatible with the UPC2
Flow-splitter kits.
Visit www.waters.com/wqp for information about optional ISM kits, including
how to order them.
Split ratio
The split ratio depends on various factors including, but not limited to, all
flow-path components downstream of the split and chromatographic
conditions (flow rate and solvent composition for both LC and make-up flows).
Restrictor modules in the ACQUITY UPLC and Alliance flow-splitter kits are
used to tune the magnitude of the split ratio. The numeric labels on the
restrictor modules correspond to the magnitude of the split ratio, as shown in
the following tables. Restrictor modules with higher numeric labels result in
higher split ratios.
Label descriptions for the ACQUITY UPLC Flow-splitter kit restrictor
module:
Restrictor module
label Description
10 Allows the largest amount of analytical flow to the
QDa detector.
100 Allows a smaller amount of analytical flow to the QDa
detector.
250 Allows the smallest amount of analytical flow to the
QDa detector.
Label descriptions for the Alliance Flow-splitter kit restrictor module:
Restrictor module
label Description
5 Allows the largest amount of analytical flow to the
QDa detector.
10 Allows a smaller amount of analytical flow to the QDa
detector.
100 Allows the smallest amount of analytical flow to the
QDa detector.

Major components
September 26, 2013, 715004208 Rev. A 19
General guidelines
When using the ACQUITY UPLC and Alliance Flow-splitter kits, observe
these guidelines:
• If the signal intensity in the QDa detector is low, switch to a restrictor
module with a lower numeric label that allows more analytical flow to
the QDa detector.
• If the signal intensity in the QDa detector is high, switch to a restrictor
module with a higher numeric label that allows less analytical flow to
the QDa detector.
The following tables provide expected ranges of mobile phase concentration at
the QDa detector (percent mobile phase in make-up flow/mobile phase
mixture entering QDa detector) over a gradient elution for a selected
restrictor module, make-up flow organic, and make-up flow composition. The
tables are valid for a make-up flow rate of 0.3 mL/min and an LC flow rate of
0.6 mL/min over a 0 to 90% organic gradient with methanol, acetonitrile, or
isopropanol.
Expected ranges of mobile phase concentration at the QDa detector
for the ACQUITY UPLC Flow-splitter kit
Restrictor module 10:
Make-up flow Mobile phase concentration at
QDa detector
Organic Composition Minimum % Maximum %
Isopropanol 10% organic 13.4 25.9
50% organic 3.8 16.0
Methanol 10% organic 14.4 27.0
50% organic 13.8 23.9
Acetonitrile 10% organic 14.6 28.5
50% organic 14.8 30.2

1 ACQUITY Isocratic Solvent Manager
20 September 26, 2013, 715004208 Rev. A
Expected ranges of mobile phase concentration at the QDa detector
for the Alliance Flow-splitter kit
Restrictor module 100:
Make-up flow Mobile phase concentration at
QDa detector
Organic Composition Minimum % Maximum %
Isopropanol 10% organic 1.5 3.9
50% organic 0.5 2.0
Methanol 10% organic 1.5 4.0
50% organic 1.4 3.6
Acetonitrile 10% organic 1.5 4.2
50% organic 1.6 4.4
Restrictor module 250:
Make-up flow Mobile phase concentration at
QDa detector
Organic Composition Minimum % Maximum %
Isopropanol 10% organic 0.6 1.6
50% organic 0.2 0.8
Methanol 10% organic 0.6 1.7
50% organic 0.6 1.5
Acetonitrile 10% organic 0.6 1.7
50% organic 0.6 1.8
Restrictor module 5:
Make-up flow Mobile phase concentration at
QDa detector
Organic Composition Minimum % Maximum %
Isopropanol 10% organic 5.2 26.5
50% organic 3.6 17.7