Woodley Skyla VB1 User manual

1VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
Skyla VB1
Quick Reference
Guide

2VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
Quick Reference Guide Contents
External features of the analyser 3
VB1 Function Buttons 4
Testing a Sample 5
How to reconstitute a biochemistry control 8
Running a Quality Control 9
Quality Control 9
Interpreting the results 10
Accessing previous results 11
Load printer paper 12
Date and Time setting 13
Troubleshooting 14
Rotor configuration and order information 17

3VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
External features of the analyser

4VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
VB1 Function Buttons
Warning –To avoid scratching the screen do not use sharp or hard objects to
touch the screen

5VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
Testing a Sample
Sample Preparation
Acceptable sample types:
Serum,
Lithium-Heparinised Whole blood or Plasma
Volume Required:
The system uses 200ul per test panel
Testing must be conducted within 60 minutes (at room temperature)
To prevent haemolysis of the sample, do not refrigerate, freeze, or shake
whole-blood sample.
Plasma or serum can be stored at Room Temp for 5 hours prior to testing or
up to 48 hours if stored at 2-8C
Performing the Test
Turn the analyser on (and off) by pressing the power on button on the front panel.
The system automatically launches the warming procedure and completes the
system self test. This may take up to 15 minutes for the system to become ready
depending on the current room temperature.
Preparing the Disc
Discs should be stored at 2-8C and can be used straight from the refrigerator without
the need for warming - Avoid placing unopened reagent discs in temperatures
greater than 25C (77F) for more than 48 hours.
1. Before using a reagent disc, carefully check the foil pouch for any damage.
Tear open the package from the upper right side of the pouch.
2. Remove the reagent disc gently with finger and thumb along with the tissue
paper from the foil pouch.
3. After removal use your other hand to hold the disc by the edge. Avoid
contact with disc surface where optical measurement takes place.
4. Unwrap and discard the tissue paper.
5. Check the reagent disc for any damage –do not use reagent discs that have
been dropped or damaged.
6. After opening the pouch the disc must be used within 20 minutes. Do not
place the disc back in the refrigerator for later use.
7. Holding the disc by the edge, gently pull the end of the aluminium strip in an
outward direction along the surface of the reagent disc; this will reveal the sample
port. Discard the aluminium strip.

6VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
Dispensing the sample
1. Attach a new pipette tip to the end of the 200ul pipette provided. Do not touch the
end of the tip.
2. Using your thump push down the plunger on the top of the pipette to the stop
position.
3. Immerse the pipette tip below the surface of the sample.
4. Slowly release the plunger to draw up the sample, avoid drawing in any air, as this
will cause the disc to fail.
5. Remove the pipette from the sample tube.
6. Insert the pipette tip into the sample port. The pipette tip should only make slight
contact with the bottom of the sample chamber, do not push down hard.
7. Keep the reagent disc level and the pipette tip perpendicular to the surface of the
disc.
8. Gently push down the plunger at the top of the pipette to the stop position. All of the
sample should have been expelled into the sample chamber. Keeping the plunger
held down gently remove the pipette tip from the sample port. The plunger may now
be released, remove the disposable tip from the pipette and discard the tip.
9. Press the START icon on the touch screen to open the reagent disc
drawer at the front of the analyzer.
10. Hold a reagent disc that contains a sample by its edge, with the barcode side
facing up and the disc kept level. Avoid touching the surface of the reagent
disc. Gently place the disc into the drawer and press OK to close the disc
drawer.
11. Use the touch screen to enter the patient ID (up to 12 numbers) or scan the patient
ID using the optional external barcode scanner and press OK
12. If you press OK without entering a patient ID, the system will use the
current date and time as the patient ID in the format dd/mm/yyyy hh:mm.

7VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
13. Select the species (feline, canine, and others).
14. The fully automated analysis will now commence once the disc drawer closes
15. When the analysis is completed, the system will store the analysis results and display
them on the screen.
To cancel a test in progress, press the CANCEL icon on the screen, the system will prompt
for confirmation. A reagent disc cannot be re used if a test has been cancelled.
Test Results
When the test is completed the system will display the results produced.
Press the Printer icon to output the results via the built in thermal printer.
Press the Home icon to return to the main menu and press OK to open the draw and
remove the used reagent disc.

8VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
How to Reconstitute a Biochemistry Control Vial
Why Run a QC?
-To assess the quality and performance of the practice laboratory.
-To ensure that results generated by the Skyla VB1 are correct.
-QC monitors staff, equipment, reagents and result reporting.
Preparing the QC reagent
1. Remove vial from the refrigerator and allow to warm at room temperature for 5
minutes
2. Carefully open and remove the bung from a vial of serum and a vial of serum diluent.
3. While holding the serum vial steady on the bench, carefully pour all the diluent into
the serum vial. Do not shake excess diluent out of the diluent vial, as this has been
accounted for in the dispense.
4. Close the serum vial, swirly gently -DO NOT shake. Let the vial stand on bench for
20 minutes then swirl again and gently invert 10 times.
5. The control is now reconstituted and ready to be analysed or frozen for prolonged
storage.
QC Storage:
Store at 2-8oC before and after reconstitution. Reconstituted control may be stored at -20oC.
Thaw frozen controls quickly using warm water (37oC), invert gently after thawing.
QC Stability:
Open vial reconstituted stability is up to 10 days if stored at 2-8oC, or 2-3 weeks if stored at -
20oC once reconstituted. Frozen controls must be used within 8 hours of thawing.

9VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
Running a Quality Control
1). Using the prepared QC sample follow the procedure as indicated in Testing a Sample for
dispensing the sample.
2). Press SETTINGS on the main menu screen.
3). Press the CONTROL icon. The test process is the same as the one for sample analysis;
however there is no need to input any patient related information.
4). The QC control test results are stored separately from sample results. The results can be
printed out after the test is completed.
5). Past QC results can be accessed using the RECALL function (See the Skyla VB1
Operator’s Manual for more details.
If you require assistance please contact your Woodley Equipment Sales
Representative or Woodley Equipment Company Technical Support on 01204
669033 and press option 1.

10 VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
Interpreting the results
The printed report consists of two sections. The heading at the top of the report includes
information about the analyser model, test date and time, sample type, patient ID, reagent
disc serial number, species, gender and weight. The results section consists of four
columns: Test marker, analyte concentration, ref range and unit. The test report also shows
the degree of Lipaemia (LIP), Haemolysis (HEM) and icterus (ICT) of the sample analysed.
Symbols Used in the test Reports:
(#) Calculated value
(*) Test result outside the reference range
(%%) The test results are significantly impacted by the interference of LIP, HEM or ICT of
the sample that is outside the permissible range of 20%
>or< Indicates that the result is outside the measurable range of the analyser. The value
displayed is either the lowest or highest value that the analyser is capable of measuring. For
Example, the measurable range of glucose is 70 –600mg/dL.. If the result is less than this
range, <70 mg/dL will be shown or higher that this range, >600 mg/dL will be shown.
(+) indicates the degree of Lipaemia (LIP), Haemolysis (HEM) and Icterus (ICT of the
sample. The more + symbols then the more severe the interference.
The classification is as follows:
0 = Clear or without interference
+ = Mild
++ = Moderate
+++ = Severe
NA indicates that the result cannot be calculated

11 VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
Accessing Previous Results
The Skyla VB1 automatically stores tests results for display, printing or Export to
USB
To access previous results: From the Home Screen > press <Recall> then <Search>
> >
Select the required search method and follow the instructions on the screen.
When the required result is located press the print icon to obtain a printout.

12 VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
Loading the Printer Paper
Open the top cover of the built-in thermal printer and place the printer paper into the
paper slot. Confirm that the heat-sensitive side is facing the correct direction (the
arrow sticker on the roll should point downwards).

13 VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
Date and Time setting
The system date and time will usually be set before installation and the system
remembers the date and time between uses.
To Change the date and time:
Select settings from the Home screen
Select administrator settings
Select Date & Time
Select the Date/Month/Year Hour/ Minute and
Use the Up and Down arrows to adjust the value.
Select OK to save the new settings

14 VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
Troubleshooting
Common Problems
Analyzer Power and Function
C = Common problem S = Solution
C: Touchscreen is black or blue; power light is OFF.
S: Turn the analyzer power off. Check the power cable connections. Disconnect and
reconnect on both ends, then turn the power on.
C: Touchscreen is blue or blank; power light is ON.
S: Remove the reagent disc from the drawer. Check
that the drawer closes properly, then re-boot the analyzer.
C: Damaged touchscreen
S: Please contact the authorized distributor or technical support department.
Settings and Reports
C: There are no values for some of the results within a printed test report, or the
system automatically cancels a reagent disc test without generating a report
S: The skyla VB1Veterinary Clinical Chemistry Analyzer performs automatic system
calibrations to maintain high system precision. Thus, when the Analyzer detects a
faulty reagent disc, it automatically cancels the test and displays an operational error
message. Please insert a new reagent disc to resume operations.
C: The test report exported by the built-in thermal printer is blank.
S: The thermal paper roll may have been installed in the wrong direction within the
built-in thermal printer. Please remove the roll of thermal paper and confirm that the
heat-sensitive side is facing the correct direction (the arrow sticker on the roll should
point downwards) before replacing the roll in the built-in thermal printer.
C: After choosing to initiate the “System Default” function, previously customized
reference range settings have disappeared.
S: Once the “System Default” function is initiated, the system will delete all custom
settings and reference ranges. Once the command is executed, all settings will be
reset to the default system settings. The settings that have been deleted cannot be
recovered. However, none of the test results and calibration records will be deleted.
Exporting data from the system to other devices
C: Peripheral and media of the exported data.
S: “Dump to USB” function: will export selected historical data to an external USB
drive. “RS232 Export” function: will export selected historical data to an RS232
station.

15 VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
Error Messages
Stage
Error Message
Error Handling
Booting up
Password Error
Please enter the correct
password
Device Abnormal (Error
Code1~20, 30, 51~56)
Please reboot the anaylzer. If
the error code cannot be
resolved, please contact the
authorized technical support
department
Date & Time Error
Please enter the correct date
and time
. Internal Memory Error
Please contact the authorized
technical support department
Printing
Internal Printer Error
Please contact the authorized
technical support department
No External Printer Found
Please check that the external
printer
is correctly connected and
whether the printer is an
analyzer compatible model
No Paper
Check if the thermal paper roll
in the built-in thermal printer is
properly installed, or add a new
roll of thermal paper.
Abnormal drawer operation
Drawer Jam
Please remove any objects that
prevent the drawer from closing
properly. Contact the authorized
technical support department if
the issue is not resolved
Testing and analysis
Internal Memory Full
Please contact the authorized
technical support department
Empty
Please insert a reagent disc
Unrecognized Disc Bar
Code(Error Code 101~104)
The reagent disc barcode
cannot be recognized. Please
insert a new reagent disc
Unauthorized Disc (Error Code
105, 106)
This is an unauthorized reagent
disc. Please contact the
authorized technical support
department
Disc Expired (Error Code 107)
The reagent disc is expired.
Please insert a new reagent
disc
Assay Parameter Version
Mismatch (Error Code 108)
Please contact the authorized
technical support department to
get the latest version of
firmware or assay parameter.
Used Disc
(Error Code 201)
Please remove the used
reagent disc
and insert a new reagent disc.
Insufficient Sample
(Error Code 204, 213)
The sample volume is
insufficient.
Please insert a new reagent
disc and make sure to inject
200 µL of sample.
Disc Error (Error Code 202,
203, 211,
212,323,355,841,842,843)
Please insert a new reagent
disc.
Device Abnormal (Error Code
3XX ,800, 801,
Please remove the reagent
disc, reboot the anaylzer, and

16 VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
802)
insert a new regent disc. If the
situation is not resolved, please
contact the authorized technical
support department.
Settings
Password Error
Please enter the correct
password
Date & Time Error
. Please enter the correct date
and time
USB Drive Error
(Error Code 411)
Please check that the USB
drive is
properly plugged into the device
Please check whether the USB
drive space is full. If the
situation is not resolved, please
contact the authorized technical
support department
No Firmware Found
There is no firmware update file
found within the USB drive.
Please contact the authorized
technical support department
for this firmware.
Upgrade Failed (Error Code
951, 953
Please contact the authorized
technical support department
Recall
No Data Matched
There is no historical data within
the system. Please conduct a
sample analysis first in order to
accumulate analysis results
within the database
RS232 communication failure
(Error Code 401, 402)
Please check that the RS232
cable is correctly connected. If
the situation is not resolved,
please contact the authorized
technical support department

17 VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
Skyla VB1 Rotors
Rotors
Parameters on Rotor
Preanaesthetic Panel (7)
ALP, ALT, TP, ALB, GLU, BUN, CREA, GLOB, A/G,
B/C
Renal Panel (7)
Na+, K+, Cl-, PHOS, ALB, BUN, CREA, Na+/K+, B/C
Liver Panel (7)
ALP, ALT, AST, TP, ALB, TBIL, AMY, GLOB, A/G, B/C
Diagnostic Panel (12)
ALP, ALT, AST, TB, GLU, BUN, CREA, TBIL, AMY,
Ca, PHOS, GLOB, A/G, B/C
Skyla VB1 Ordering Information
Product Code
Description
WD0590
Skyla VB1 Veterinary Chemistry Analyser
WD0591
Skyla VB1 Veterinary Chemistry Rotor -
Preanaesthetic Panel
WD0592
Skyla VB1 Veterinary Chemistry Rotor - Renal
Panel
WD0593
Skyla VB1 Veterinary Chemistry Rotor - Liver Panel
WD0594
Skyla VB1 Veterinary Chemistry Rotor - Diagnosis
Panel