5VB1 DM/JAC 2014 v1.0
This document is a quick reference guide. For more comprehensive
information please refer to your Skyla VB1 Operator’s Manual
Testing a Sample
Sample Preparation
Acceptable sample types:
Serum,
Lithium-Heparinised Whole blood or Plasma
Volume Required:
The system uses 200ul per test panel
Testing must be conducted within 60 minutes (at room temperature)
To prevent haemolysis of the sample, do not refrigerate, freeze, or shake
whole-blood sample.
Plasma or serum can be stored at Room Temp for 5 hours prior to testing or
up to 48 hours if stored at 2-8C
Performing the Test
Turn the analyser on (and off) by pressing the power on button on the front panel.
The system automatically launches the warming procedure and completes the
system self test. This may take up to 15 minutes for the system to become ready
depending on the current room temperature.
Preparing the Disc
Discs should be stored at 2-8C and can be used straight from the refrigerator without
the need for warming - Avoid placing unopened reagent discs in temperatures
greater than 25C (77F) for more than 48 hours.
1. Before using a reagent disc, carefully check the foil pouch for any damage.
Tear open the package from the upper right side of the pouch.
2. Remove the reagent disc gently with finger and thumb along with the tissue
paper from the foil pouch.
3. After removal use your other hand to hold the disc by the edge. Avoid
contact with disc surface where optical measurement takes place.
4. Unwrap and discard the tissue paper.
5. Check the reagent disc for any damage –do not use reagent discs that have
been dropped or damaged.
6. After opening the pouch the disc must be used within 20 minutes. Do not
place the disc back in the refrigerator for later use.
7. Holding the disc by the edge, gently pull the end of the aluminium strip in an
outward direction along the surface of the reagent disc; this will reveal the sample
port. Discard the aluminium strip.
