Woodley InSight V3 PLUS User manual

Veterinary Haematology Analyser
Operator’s Manual

NOTE
1) Please read this manual carefully before operating the analyser.
2) Inspect the electrical requirements of the analyser before powering on
and properly connect the grounding wire.
3) Turn off the power to the analyser and disconnect the power cord if the
analyser is idle for a long time.
4) Do not run the analyser if it’s in an abnormal or damaged condition.
5) There is potential biohazard of the reagents and samples; the operator
should follow the correct biosafety practices. Dispose of waste reagent
and sample in accordance with local, national regulations.

Contents
Copyright and Declaration...................................................................................... 1
Guidance................................................................................................................... 3
Chapter 1 System Description................................................................................ 4
1.1 Overview ................................................................................................. 4
1.1.1 Function....................................................................................... 4
1.1.2 Intended Use............................................................................... 4
1.1.3 Front Panel.................................................................................. 5
1.1.4 Side & Rear Panels..................................................................... 8
1.2 Parameters.............................................................................................. 9
1.3 Structure ............................................................................................... 10
1.3.1 Flow System.............................................................................. 10
1.3.2 Electrical System....................................................................... 10
1.3.3 Display...................................................................................... 11
1.4 Accessories........................................................................................... 11
1.5 Sample Volume..................................................................................... 11
1.6 Reagent Volume for Single Sample....................................................... 12
1.7 Test Speed............................................................................................ 12
1.8 Storage……………………………………………………………….. .......... 12
1.9 Background ........................................................................................... 12
1.10 Carryover............................................................................................... 12
1.11 Repeatability.......................................................................................... 12
1.12 Linearity ................................................................................................ 13
1.13 Transport and Storage Specifications.................................................... 13
1.14 Environment Requirement..................................................................... 13
1.15 Electrical Requirement .......................................................................... 13
1.16 Reagent................................................................................................. 14
1.16.1 Diluent..................................................................................... 14
1.16.2 Lyse ........................................................................................ 14
1.16.3 Probe Detergent...................................................................... 15
1.16.4 Note of Reagent Use............................................................... 15
1.16.5 Reagent Storage..................................................................... 15
Chapter 2 Principles of Operation........................................................................ 16
2.1 Test Principles....................................................................................... 16
2.1.1 Electrical Impedance Principle for Blood Cells.......................... 16
2.1.2 Colourimetry Principle for HGB Metering.................................. 17
2.2 Calculation of Parameters ..................................................................... 17
Chapter 3 Installation and Specimen Analysis................................................... 20

3.1 Unpacking and Inspection ..................................................................... 20
3.2 Installation Requirements...................................................................... 20
3.3 Power Supply Inspection....................................................................... 21
3.4 Tubing Installation ................................................................................. 21
3.4.1 LYSE Tubing Installation........................................................... 21
3.4.2 DILUENT Tubing Installation..................................................... 21
3.4.3 WASTE Tubing Installation........................................................ 22
3.5 Printer Installation (Optional)................................................................. 22
3.6 Keyboard and Mouse Installation (Optional).......................................... 22
3.7 Power Connection................................................................................. 23
3.8 Startup………………………………………………………………….......... 23
3.9 Background Test ................................................................................... 24
3.10 Quality Control....................................................................................... 25
3.11 Calibration ............................................................................................. 25
3.12 Collection of Blood Sample ................................................................... 25
3.12.1 Whole Blood Collection ........................................................... 26
3.12.2 Peripheral Blood Collection (Pre-diluent)................................. 26
3.13 Mode Switch.......................................................................................... 26
3.14 Sample Counting and Analysis.............................................................. 26
3.14.1 Information Input...................................................................... 26
3.14.2 Counting and Analysis............................................................. 28
3.14.3 Special Function...................................................................... 29
3.15 Result Analysis...................................................................................... 30
3.16 Report Output........................................................................................ 30
3.17 Result Modification................................................................................ 31
3.18 Shutdown .............................................................................................. 32
3.19 Data Query............................................................................................ 33
3.19.1 Data Query.............................................................................. 33
3.19.2 Data Selection......................................................................... 34
3.19.3 Data Deletion........................................................................... 35
3.19.4 Edit Information ....................................................................... 35
3.20 Special Function.................................................................................... 36
3.20.1 Export...................................................................................... 36
3.20.2 CV Value and Trend graph...................................................... 37
Chapter 4 System Setting..................................................................................... 39
4.1 Settings………………………………………………………………............ 39
4.2 System Maintenance............................................................................. 39
4.3 Limit……………………………………………………………………. ......... 40
4.4 Time…………………………………………………………………….......... 41
4.5 Parameter.............................................................................................. 41
4.6 Print…………………………………………………………………… .......... 42

4.7 Transmit ................................................................................................ 43
4.8 Maintenance.......................................................................................... 44
4.9 Version………………………………………………………………… ......... 45
4.10 User…………………………………………………………………….. ........ 45
4.11 Service .................................................................................................. 47
4.12 Reagents............................................................................................... 47
Chapter 5 Quality Control ..................................................................................... 49
5.1 Quality Control Options ......................................................................... 49
5.2 QC Mode Select.................................................................................... 50
5.3 L-J QC………………………………………………………………….......... 51
5.3.1 L-J QC Setting........................................................................... 51
5.3.2 L-J QC List ................................................................................ 55
Chapter 6 Calibration ............................................................................................ 56
6.1 Calibration Frequency ........................................................................... 56
6.2 Preparation............................................................................................ 57
6.3 Calibration Modes.................................................................................. 58
6.3.1 Manual Calibration.................................................................... 58
6.3.2 Standard Calibration ................................................................. 59
6.3.3 Blood Calibration....................................................................... 61
Chapter 7 Reference Ranges................................................................................ 63
7.1 Limit Review.......................................................................................... 63
7.2 Range Modification................................................................................ 64
7.3 Print…………………………………………………………………… .......... 65
Chapter 8 Maintenance ......................................................................................... 66
8.1 Daily Maintenance................................................................................. 66
8.2 Weekly Maintenance............................................................................. 67
8.2.1 Surface Maintenance................................................................ 67
8.3 System Maintenance............................................................................. 67
8.3.1 Zap Aperture............................................................................. 68
8.3.2 Flush Aperture........................................................................... 68
8.3.3 Draining Cups ........................................................................... 69
8.3.4 Clean Cups............................................................................... 69
8.3.5 Rinsing Cups............................................................................. 69
8.3.6 Change Lyse............................................................................. 70
8.3.7 Change Diluent ......................................................................... 70
8.3.8 Clean Rear Chamber................................................................ 70
8.3.9 Prime......................................................................................... 71
8.3.10 Prepare Shipping .................................................................... 71
8.4 Maintenance before Shipping................................................................ 72
Chapter 9 Service .................................................................................................. 74
9.1 System Check ....................................................................................... 74
9.1.1 System Status Check................................................................ 74

9.1.2 Valve Check.............................................................................. 75
9.1.3 Motor Check.............................................................................. 75
9.2 System Log............................................................................................ 76
9.2.1 Date Query................................................................................ 77
Chapter 10 Troubleshooting................................................................................. 78
10.1 Troubleshooting Guidance .................................................................... 78
10.2 Obtaining Technical Assistance............................................................. 78
10.3 Troubleshooting..................................................................................... 78
10.3.1 Faults Related to Reagents..................................................... 79
10.3.2 Faults Related to Vacuum....................................................... 79
10.3.3 Faults Related to 5V Voltage................................................... 80
10.3.4 Faults Related to Test Value................................................... 80
10.3.5 Faults Related to Hardware..................................................... 81
10.3.6 Faults Related to Temperature................................................ 82
Chapter 11 Precautions, Limitations and Hazards............................................. 83
11.1 Limitations ............................................................................................. 83
11.2 Location Limitations............................................................................... 83
11.3 Safety and Infection Control.................................................................. 84
Appendix A: Analyser Specifications .................................................................. 85
Appendix B: Analyser Icons and Symbols......................................................... 88
Appendix C: Communication................................................................................ 89
1 Hexadecimal Format Communication.................................................... 89
1.1 Data Link MAC Sublayer Parameters Convention........................ 89
1.2 Data Link Layer Frame Format..................................................... 89
1.2.1 Frame Format.............................................................................. 89
1.2.2 Meaning of Each Field or Control Field ....................................... 89
1.2.3 Convention.................................................................................. 90
1.3 Message Field Structure .............................................................. 90
1.3.1 Message Structure ...................................................................... 90
1.3.2 DATA Field Definition................................................................ 90
2ASCII Format Communication............................................................... 90
2.1 Message Transfer Format.................................................................................. 90
2.2 Message Grammar ............................................................................................ 91
2.3 Data Type .......................................................................................................... 91
2.4 Message Type ................................................................................................... 92
3Communication Operations................................................................... 97

1
Copyright and Declaration
Copyright © Woodley Equipment Company Ltd.
Declaration:
All contents in this manual were strictly compiled according to related lawsand
regulations as well as the specific condition of the InSight V3®Plus Veterinary
Haematology Analyser, covering all the updated information before printing.
Woodley Equipment Company Ltd is fully responsible for the revision and
explanation of the manual and reserves the right to update the relevant contents
without separate notification. All images are for illustrative purposes only.
All the information included is protected by copyright. No part of this document
may be reproduced, stored or transmitted in any form or by any means unless
with written authorisation by Woodley Equipment Company Ltd.
All instructions must be followed strictly during operation. In no event is
Woodley Equipment Company Ltd responsible for failures, errors and other
liabilities resulting from user's non-compliance with the procedures and
precautions outlined herein.
Limited Responsibility for Quality Warranty:
The manual for the InSight V3 Plus Veterinary Haematology Analyser defines
the rights and obligations between the manufacturer and the customer about
the responsibility for quality warranty and after-sales service, also the related
agreements on commencement and termination.
Woodley Equipment Company warrants the InSight V3 Plus supplied by
Woodley Equipment Company and its authorised agents to be free from defects
in workmanship and materials during normal use by the original purchaser.
Woodley Equipment Company assumes no liability in the following situations:

2
1) Failure due to abuse of the instrument or neglecting the maintenance.
2) Use of reagents and accessories other than those manufactured or
recommended by Woodley Equipment Company.
3) Failure due to operation which is not under the instructions described in the
manual.
4) Replacement of accessories which are not specified by Woodley
Equipment Company. Maintenance or repair of the analyser by a service
agent who is not approved or authorised byWoodley Equipment Company.
5) Components have been dismounted, stretched or readjusted.
CAUTION:
THE ANALYSER IS FOR VETERINARY USE ONLY.
Technical service and troubleshooting are provided by the Service Department
and Technical Support Department of Woodley Equipment Company.
Woodley Equipment Company Ltd.
Old Station Park Buildings, St. John Street, Horwich, Bolton,
Lancashire, BL6 7NY, United Kingdom
Tel: [phone]
Fax: [phone]
Web: www.woodleyequipment.com
Email: [email protected]
Version: 03/2021

3
Guidance
General information for the operation of the analyser is contained in this manual
which covers the best guidance for a new operator to understand the
characteristics of the analyser and its operation methods, as well as for daily
enquiry. Please read the InSight V3 Plus Operator’s Manual thoroughly before
using the analyser.
This manual uses the following warning conventions:
WARNING: Denotes a hazard which, if not avoided, could result in moderate
to serious injury.
CAUTION: Denotes potential hazards that could result in a minor injury, also
used for conditions or activities which could interfere with proper
function of the analyser.
NOTE: Denotes special operator/service information or standard practices.
Please read this manual before operating the analyser.

4
Chapter 1 System Description
1.1 Overview
The InSight V3 Plus is a multi-parameter automated Veterinary Haematology
analyser, designed for in vitro diagnostic use in clinical laboratories to analyse
blood cells.
1.1.1 Function
The InSight V3 Plus uses Coulter electrical impedance and colourimetry
methods to test the WBC, RBC, PLT, MCV and HGB parameters, three WBC
differentials with eosinophil estimation and provides histogram information.
1.1.2 Intended Use
The InSight V3 Plus is a quantitative, automated haematology analyser and 3-
part differential counter for animal blood samples.

5
1.1.3 Front Panel
Figure 1-1
1. Status Indicators
Standby Indicator: Indicator light turns orange; the analyser is in sleep mode.
Ready to Test Indicator: Indicator light turns green; the analyser isready to
run a sample.
Alarm Indicator: Indicator light turns red; the analyser has an error.
2. Aspiration Probe
Aspirate samples.
3. RUN Key
Press the RUN key to start up the aspiration probe and then analyse the
specimen when the Main Menu and Quality Control screens are displayed. On
other screens, the RUN key is invalid.
4. Display
10.4-inch colour LCD. The screen is divided into 7 areas as shown in Figure 1-
2.

6
(1) Function Buttons Area
Display function buttons. There are three sets of function
buttons. The first set. See Figure 1-3.
Test: Display the main interface and run a test
Data: Enter data storage interface and query sample results
QC: Enter the QC interface to run quality control
Cal: Enter the calibration interface to run calibration
Setup: Enter the setup interface to set system parameters
The second set. See Figure 1-4:
Figure 1-4
Next sample: New sample details
Mode switch: Switch the test mode to whole blood mode or diluent mode
Audit: Audit the sample
Diluent: Dispense 0.8ml from sample probe into a plain tube for
Figure 1-2
Figure 1-3

7
background test or to dilute blood sample
The third set. See Figure 1-5:
Select or to enter the third set menu. See Figure 1-5:
Figure 1-5
Pre. record: To see the previous record
Next record: To see the next record. If the current record is the last one, the
button will be grey
Audit: Audit the sample
Edit Result: Modify sample results
Print: Print the sample results
Transmit: Transmit sample data to LIS
(2) Fault Prompt
System fault errors and information.
(3) Analysis Mode
Select and indicate the system running state: whole blood sampling mode or
pre-dilute mode.
(4) Patient Information
Display the information of patient sample.
(5) Parameter Information Display
Display each parameter results.
(6) Graphic Display
Display the histograms of WBC, RBC and PLT.
(7) Status Area
Display the current time, date, operator, next sample number and printer status.

8
1.1.4 Side & Rear Panels
Figure 1-7
1. COM
Connect to the standard RS-232 network.
2. USB Port
Connect to USB equipment.
3. Grounding Terminal
Used to ground the analyser.
4. Power Input
Connect the main power cord to the analyser.
5. Power Switch
Turn the power supply on or off.
6. Thermal Printer
Print the test result.
7. LYSE
Lyse port connects to the lyse inlet tube.
8. SENSOR
Connect to the waste sensor.
9. WASTE
Waste port connects to the waste outlet tube.
10. DILUENT
Diluent port connects to the diluent inlet tube.

9
1.2 Parameters
The analyser automatically analyses the sample data and displays the
histogram of WBC (3 part differential count), RBC and PLT.
Table 1-1
Abbreviation
Full Name
Unit
WBC
White Blood Cell Count
109cells/L
LYM%
Lymphocyte Percent
%
MID%
Monocyte Percent
%
GRAN%
Granulocyte Percent
%
LYM#
Lymphocyte Count
109cells/L
MID#
Monocyte Count
109cells/L
GRAN#
Granulocyte Count
109cells/L
RBC
Red Blood Cell Count
1012cells/L
HGB
Haemoglobin Concentration
g/L（or g/dL）
HCT
Haematocrit (relative
volume of erythrocytes)
%
MCV
Mean Corpuscular Volume
fL
MCH
Mean Corpuscular
Haemoglobin
pg
MCHC
Mean Corpuscular
Haemoglobin Concentration
g/L（or g/dL）
RDW_CV
Red Blood Cell Distribution
Width repeat precision
%
RDW_SD
Red Blood Cell Distribution
Width STDEV
fL
PLT
Platelet Count
109cells/L
MPV
Mean Platelet Volume
fL
PDW
Platelet Distribution Width
fL
PCT
Plateletcrit
%
P_LCR
Large Platelet Ratio
%
P_LCC
Large Platelet
109cells/L

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1.3 Structure
The analyser consists of a flow system, electrical system and display.
1.3.1 Flow System
The flow system is composed of solenoid valves, a vacuum pump and a plastic
tube.
Solenoid Valve –These two-way or three-way solenoid valves control the flow
of reagent.
Vacuum Pump –Pump the waste out of the analyser and produce negative
pressure.
Plastic Tube –Reagent and waste flow in the plastic tube.
Vacuum Chamber –Generate negative pressure and play the role of
temporary waste reservoir. It can also generate positive pressure when
flushing.
1.3.2 Electrical System
1.3.2.1A/D and Central Control Board
The central control board is the control centre of the analyser. It controls the
following parts:
•The opening and closure of all valves, reagent aspiration, rinse and waste
discharge
•Run force pump and vacuum pump to mix reagent
•Eliminate clogs, aspirate and discharge reagents
•Control step motors to aspirate sample and reagent
•Control the A/D conversion of WBC, RBC/PLT and HGB
•Check all the optical and electrical switch movements
1.3.2.2 WBC/RBC/PLT Metering Assembly
WBC Metering Assembly is composed of a signal collection board, electrodes,
micro-aperture sensor and flow system.
•Signals Collection Board –Provides electrodes constant current, amplifies and
deals with the collected pulse signal for mainboard.
•Electrodes –There are two electrodes in the sample cup, one inner electrode
located in the front chamber and one outer electrode located in the back
chamber. Both electrodes are submerged in the conductive liquid, creating an
electrical pathway through the micro-aperture.

11
•Micro-aperture Sensor –Micro-aperture sensor is mounted on the front of the
sample cup. The diameter of WBC and RBC/PLT metering assembly is 100µm
and 68µm respectively.
•Flow System –The flow system uses negative pressure to aspirate diluent and
sample from each container into the metering tube and discharge waste at the
end of the processing. There is a lyse adding and mixing unit in front of the
sample cup. The control board controls the step motor. When testing the WBC,
the lyse will be added into the sample cup and then the vacuum pump will
generate compressed gas to mix the samples.
1.3.3 Display
InSight V3 Plus uses a 10.4-inch colour LCD which displays 21 parameters
(with 3 histograms).
1.4 Accessories
Please see InSight V3 Plus packing list.
1.5 Sample Volume
Whole Blood Mode: Whole Blood (Venous Blood) 8.5µl
Pre-Dilute Mode: Diluted Blood 20µl

12
1.6 Reagent Volume for Single Sample
Diluent: 25mL
Lyse: 0.4mL
NOTE: Reagent consumption varies according to the software version.
1.7 Test Speed
InSight V3 Plus is able to process 55 samples per hour.
1.8 Storage
InSight V3 Plus can store 100,000 results.
1.9 Background
WBC≤0.2×109/L；RBC≤0.02×1012/L；HGB≤1g/L；PLT≤10×109/L.
1.10 Carryover
WBC≤0.5%；RBC≤0.5%；HGB≤0.6%；PLT≤1.0%.
1.11 Repeatability
The precision of the analyser is shown in Table 1-3.
Table 1-3
Parameter
Acceptable Limits
（CV/%）
Precision Range
WBC
≤3.5%
4.0×109/L ~ 15.0×109/L
RBC
≤1.5%
3.00×1012/L
~6.00×1012/L
HGB
≤1.5%
100 g/L ~180g/L
HCT
≤2.0%
35%~50%
MCV
≤1.0%
76fL ~110fL
PLT
≤4.0%
100×109/L ~500×109/L

13
1.12 Linearity
The linearity of the analyser is shown in Table 1-4.
Table 1-4
Parameter
Linearity Range
Acceptable Limits
WBC
0×109/L~10.0×109/L
≤±0.3×109/L
10.1×109/L ~99.9×109/L
≤±5%
RBC
0×1012/L ~1.00×1012/L
≤±0.05×1012/ L
1.01×1012/L ~9.99×1012/L
≤±5%
HGB
0 g/L ~70 g/L
≤±2g/L
71 g/L ~300 g/L
≤±2%
PLT
0×109/L ~100×109/L
≤±10×109/L
101×109/L ~999×109/L
≤±10%
MCV
25 –120
≤±1%
1.13 Transport and Storage Specifications
a) Temperature: -10℃~55℃
b) Relative Humidity: ≤95%RH
c) Barometric: 50kPa~106kPa
1.14 Environment Requirement
a) Temperature: 10~35°C
b) Relative Humidity: ≤90%RH
c) Barometric: 60kPa~106kPa
1.15 Electrical Requirement
Power Supply: AC 100V～240V
Frequency: 50/60Hz
Power: 130VA-180VA
Fuse: 250V/3.15A

14
1.16 Reagent
The reagent is formulated specifically for the InSight V3 Plus flow systems in
order to provide optimal system performance. Use of reagents other than those
specified in this manual is not recommended as analyser performance can be
affected. Each InSight V3 Plus is checked using the specified reagents and all
performance claims were generated using these reagents.
Reagents must be stored at room temperature to ensure optimal performance.
All reagents should be protected from direct sunlight, extreme heat, and
freezing during storage. Temperatures below 0°C may cause reagent layering
and abnormal results.
The reagent inlet tubes have a cap attached that minimises evaporation and
contamination during use. However, reagent quality may deteriorate with time.
Therefore, use all reagents within the dating period.
1.16.1 Diluent
Diluent is a buffer solution. The InSight V3 Plus Diluent:
a) Dilutes WBC, RBC, PLT and HGB
b) Maintains cell volume during the test process
c) Offers an appropriate background value
d) Cleans WBC and RBC micro-aperture and tubes
1.16.2 Lyse
The InSight V3 Lyse Reagent:
a) Lyses RBC with minimum ground substance complex.
b) Lyses the membrane of the WBC to diffuse the cytoplasm, leaving the
remaining WBC nuclei.
c) Transforms the haemoglobin to the haemo-compound which is suitable for
measurement by a spectrophotometer at a wavelength of 540nm.