Woodley Insight User manual

Insight Electrolyte Analyser
Quick Reference Guide

2
This document is a Quick Reference Guide. For more comprehensive information please refer to the Insight
Electrolytes Operators Manual
Quick Reference Guide Contents
3
Setup and Installing the Electrodes
5
Electrode Configurations
6
Start Up
7
Running a Sample
8
How to Run the Quality Control
9
Result Review
10-14
Maintenance
15
Shutdown
17
Reference Ranges

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This document is a Quick Reference Guide. For more comprehensive information please refer to the Insight
Electrolytes Operators Manual
Setup and Installing the Electrodes
1. Remove the red transport clips and loop the tubing
around the white pump roller
2. Remove the reference housing and the electrodes from
their protective boxes and place on a clean surface. Make
sure each electrode has a black o-ring on the left side.
3. Unscrew the transport housing from the reference
electrode and check that the o-ring on the electrode is
properly seated. Rinse, dry and save the transport
housing.
4. Carefully screw the reference electrode into the
reference electrode housing and place it with the other
electrodes. Check for fill solution in the electrodes and top
up if required with the enclosed fill solution (see
unscheduled maintenance section, page 14).

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This document is a Quick Reference Guide. For more comprehensive information please refer to the Insight
Electrolytes Operators Manual
Setup and Installing the Electrodes Continued...
5. Slide the measurement chamber forward until it locks in the front position.
6. Unclamp the electrode holder on the left by moving the clamp forwards.
7. Check that an o-ring is present in the electrode holder on the right.
8. Install the electrodes into their corresponding positions
starting from the right hand side and working to the left (the
reference electrode will be installed last). The electrodes have
a lip on the bottom that rests on the flat edge of the
measurement chamber.
8. When all electrodes have been installed, close the clamp on the electrode holder on the left side
by lifting it upwards until it locks in the back position.
9. Slide the measurement chamber back until it snaps into position.
10. Plug the tubing connector of the reference housing assembly in the receptacle below the left
side of the measurement chamber.

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This document is a Quick Reference Guide. For more comprehensive information please refer to the Insight
Electrolytes Operators Manual
Electrode Configurations
Possible parameter configurations of the electrodes are:
Electrode Position
Na
K
Ca/Cl/Li
1
Na
K
Dummy
2
Na
K
Cl
3
Na
Dummy
Cl
4
Na
K
Ca
5
Na
Dummy
Ca
6
Na
K
Li
7
Na
Dummy
Li
8
[Na]
Dummy
Li
9
Na
Ca
Li

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This document is a Quick Reference Guide. For more comprehensive information please refer to the Insight
Electrolytes Operators Manual
Start Up
1. Switch on the analyser. After 60 seconds the analyser will display “Standby on”. Press “Yes” and
the analyser will display “Deactivate standby?”. Press “Yes” to confirm.
2. When using the analyser for the first time every day, it is advised to perform a Daily Clean. The
analyser will display “Clean”.
Pour a small amount of the „ISE Cleaning Solution‟ into a clean tube. Press „Yes‟
“Lift door load sample” will be displayed. Open the sample door and introduce the
solution to the probe.
“Wipe probe_shut sample door” will be displayed. Wipe the probe with a lint-free tissue
and close the door.
3. The analyser will display “Condition”.
Pour a small amount of the „Electrode Conditioning Solution‟ into a clean tube. Press
„Yes‟
“Lift door load sample” will be displayed. Open the sample door and introduce the
solution to the probe.
“Wipe probe_shut sample door” will be displayed. Wipe the probe with a lint-free tissue
and close the door.
4. The analyser will display “Continue maintenance”. Press „No‟ and the analyser will begin an
automatic calibration cycle.
5. After finishing the calibration cycle, the analyser displays “H-Ready Na-K”. Other parameters will
be displayed if they are set up on the analyser. The analyser is now ready for sample analysis.

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This document is a Quick Reference Guide. For more comprehensive information please refer to the Insight
Electrolytes Operators Manual
Running a Sample
1. Sample types:
Whole blood - from a heparinised syringe or capillary tube.
Serum - by centrifuging either a plain tube or a gel tube.
Plasma - by centrifuging heparinised whole blood.
Urine - A dilution with “Urine diluent” must be performed before running urine samples.
2. To enter patient details press „No‟ until “QC/STD/Urine Sample?” is displayed.
3. Press „Yes‟ to confirm and then press „No‟ until “Standard sample” is displayed.
4. Press „Yes‟ and enter the patient details using the keyboard and press Enter. Press ‟Yes‟ to
confirm.
5. Open the sample door and “Load sample” will be displayed. Introduce the sample to the probe
until “Wipe probe_shut sample door” is displayed.
6. Wipe the probe with a lint-free tissue and close the door.
7. When analysis is complete, the results will be printed by the integrated printer.

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This document is a Quick Reference Guide. For more comprehensive information please refer to the Insight
Electrolytes Operators Manual
How to Run Quality Control
Reconstitution Procedure
1. Remove the control vial from the refrigerator and allow to warm at room temperature for 5
minutes.
2. Remove the stopper and add 5ml DIL SIM diluent to the control vial.
3. Replace stopper and swirl vial gently. DO NOT shake.
4. Let the vial stand on bench for 20 minutes then swirl again and gently invert 10 times.
5. The control is now reconstituted and ready to be analysed or frozen for prolonged storage.
QC Storage
Store at 2-8oC before and after reconstitution. Reconstituted control may be stored at -20oC. Thaw
frozen controls quickly using warm water (37oC), invert gently after thawing.
QC Stability
Open vial reconstituted stability is up to 10 days if stored at 2-8oC, or 2-3 weeks if stored at -20oC
once reconstituted. Frozen controls must be used within 8 hours of thawing.
Running the QC
1. Prepare QC and allow the QC to reach room temperature.
2. Gently swirl to mix the QC material.
3. Run the QC in the same way that you would a sample (refer to page 7).
4. Compare the results to reference sheet provided with the control.
5. Sign the QC log under the appropriate date.
In the event of a QC failure:
1. Reprocess the sample
2. If still out of limits, repeat with fresh QC
3. Contact your Woodley Account Manger or Technical support on 01204 669033

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This document is a Quick Reference Guide. For more comprehensive information please refer to the Insight
Electrolytes Operators Manual
Result Review
Results can be printed retrospectively if required from memory. The analyser can store up to 1000
tests. To print stored results follow these steps:
1. Press „No‟and “Print reports?” will be displayed.
2. Press „Yes‟ and “Print last sample?” will be displayed.
If you want to print the last run then press „Yes‟. The results will be displayed and the report
will print.
If you want to print a different results press „No‟.
3. If „No‟ is selected “Print by sample ID” will be displayed.
Press „Yes‟and the prompt “Enter ID” will be displayed.
Press „No‟to scroll through the numbers or press „Yes‟to enter the correct ID using the
keyboard.
Press „Yes‟to confirm and the analyser will begin printing.
“Find more” is displayed. Press „Yes‟ to find more results for the same sample ID or press
„No‟ to move to the next menu option.
4. If „No‟ is selected “Print by name” is displayed.
Press „Yes‟ and then use the „No‟ button to scroll through letters or use the keyboard.
When the name is displayed press „Yes‟ to print.
“Find more” is displayed. Press „Yes‟ to print more results from the same patient name or
„No‟ to move to the next menu option.
5. If „No‟ is selected “Print by test” is displayed.
Press „Yes‟ and “Enter test #” is displayed
Press „No‟ to scroll through the numbers
When the correct number is displayed press „Yes‟ to print.
“Find more” is displayed. Press „Yes‟ to find more results for the same test number or press
„No‟ to move to the next menu option.
10. If „No‟ is selected “Print by date?” is displayed.
This will print results for a specific day.
11. “Daily summary” will print the last day‟s results.
12. “Weekly summary” will print the results from the previous 5 days.
13. “Print all” will print all data stored. This function can be interrupted by pressing „No‟.

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This document is a Quick Reference Guide. For more comprehensive information please refer to the Insight
Electrolytes Operators Manual
Maintenance
Daily Maintenance
Please refer to „Start Up‟ on page 6 for the daily clean instructions.
Weekly Maintenance
Clean sample probe and fill port:
From the “H-READY” screen open the sample door.
Clean the fill port, probe and surrounding area with a
damp cotton swab.
If the analyser attempts to perform sample analysis
“Sample not found” will briefly display but then return to
“H-READY”.
The exterior surfaces should be wiped with a soft, damp
cloth.
Monthly Maintenance
Clean the reference electrode housing:
From the “H-READY” screen, press „No‟ to access “Maintenance?”.
Press „Yes‟ and “Clean?” appears, DO NOT PRESS ANY KEYS.
Open the front cover of the analyser.
Unplug the tubing of the reference electrode from the receptacle below the left side of the
measuring chamber.
Slide the measuring chamber forward until it locks in the front position.
Unclamp the left electrode holder by moving the clamp forward.