wtw photolab S12 User manual

OPERATING MANUAL
ba75433e0906/2014
photoLab®S12
ANALYSIS SPECIFICATIONS FOR THE AVAILABLE TEST KITS

Contents
Table – Available photometric test kits
Analytical Procedures
Appendix 1 – Suitability of Test Kits for Testing Seawater
and Tolerance Limits of Neutral Salts
Appendix 2 – Spectroquant®CombiCheck and Standard
Solutions
Appendix 3 – Instructions for the Preparation of Standard
Solutions
photoLab®Series

The following methods are programmed into the photometer and measurements can be made without any
further adjustments. Method selection is achieved through a barcode on the cell (for cell tests) or through a
barcode on the AutoSelector (for reagent tests). The method number listed in column 1 is for manual selection.
The total range relates to the cited test in column 2 and, in the reagent tests, covers all possible path length
(cells from 10 to 50 mm).
Release 06/2014
photoLab®S12
Available photometric test kits
Method Determination Total Range Method
No.
003 Ammonium Cell Test A6/25 0.20 – 8.00 mg/l NH4-N Indophenol blue
104 Ammonium Cell Test 114739 0.010 – 2.000 mg/l NH4-N Indophenol blue
052 Ammonium Cell Test 114544 0.5 – 16.0 mg/l NH4-N Indophenol blue
053 Ammonium Cell Test 114559 4.0 – 80.0 mg/l NH4-N Indophenol blue
095 Chloride Cell Test* 114730 5 – 125 mg/l Cl Iron(III)-thiocyanat
039 Chromate Cell Test* 114552 0.05 – 2.00 mg/l Cr Diphenylcarbazide
039 Chromate Cell Test* 114552 0.05 – 2.00 mg/l Cr Peroxodisulfate oxidation,
(total chromium) diphenylcarbazide
001 COD Cell Test* C3/25 10 – 150 mg/l COD Chromosulfuric acid oxidation,
chromate determination
031 COD Cell Test* 114560 4.0 – 40.0 mg/l COD Chromosulfuric acid oxidation,
chromate determination
105 COD Cell Test* 114895 15 – 300 mg/l COD Chromosulfuric acid oxidation,
chromate determination
093 COD Cell Test* 114690 50 – 500 mg/l COD Chromosulfuric acid oxidation,
chromate determination
002 COD Cell Test* C4/25 25 – 1500 mg/l COD Chromosulfuric acid oxidation,
chromium(III) determination
094 COD Cell Test* 114691 300 – 3500 mg/l COD Chromosulfuric acid oxidation,
chromium(III) determination
024 COD Cell Test* 114555 500 – 10000 mg/l COD Chromosulfuric acid oxidation,
chromium(III) determination
026 Copper Cell Test* 114553 0.05 – 8.00 mg/l Cu Cuprizone
037 Iron Cell Test 114549 0.05 – 4.00 mg/l Fe Triazine
017 Nickel Cell Test* 114554 0.10 – 6.00 mg/l Ni Dimethylglyoxime
004 Nitrate Cell Test* N2/25 0.5 – 25.0 mg/l NO3-N 2,6-Dimethylphenol
059 Nitrate Cell Test* 114542 0.5 – 18.0 mg/l NO3-N Nitrospectral
107 Nitrate Cell Test* 114764 1.0 – 50.0 mg/l NO3-N 2,6-Dimethylphenol
072 Nitrate Cell Test in seawater* 114556 0.10 – 3.00 mg/l NO3-N Resorcine
005 Nitrite Cell Test* N5/25 0.010 – 0.700 mg/l NO2-N Griess reaction
068 Nitrogen (total) Cell Test 114537 0.5 – 15.0 mg/l N Peroxodisulfate oxidation, nitrospectral
153 Nitrogen (total) Cell Test* 100613 0.5 – 15.0 mg/l N Peroxodisulfate oxidation,
2,6-dimethylphenol
108 Nitrogen (total) Cell Test 114763 10 – 150 mg/l N Peroxodisulfate oxidation,
2,6-dimethylphenol
006 Phosphate Cell Test P6/25 0.05 – 5.00 mg/l PO4-P Phosphomolybdenum blue
006 Phosphate Cell Test P6/25 0.05 – 5.00 mg/l P Peroxodisulfate oxidation,
(total phosphorus) Phosphomolybdenum blue
007 Phosphate Cell Test P7/25 0.5 – 25.0 mg/l PO4-P Phosphomolybdenum blue
007 Phosphate Cell Test P7/25 0.5 – 25.0 mg/l P Peroxodisulfate oxidation,
(total phosphorus) Phosphomolybdenum blue
103 Potassium Cell Test 114562 5.0 – 50.0 mg/l K Kalignost, turbidimetric
064 Sulfate Cell Test 114548 5 – 250 mg/l SO4Bariumsulfate, turbidimetric
082 Sulfate Cell Test 114564 100 – 1000 mg/l SO4Bariumsulfate, turbidimetric
074 Zinc Cell Test 114566 0.20 – 5.00 mg/l Zn PAR
208 Acid Capacity Cell Test to pH 4.3 101758 0.40 – 8.00 mmol/l Indicator reaction
(total alkalinity)
196 Aluminium Cell Test* 100594 0.02 – 0.50 mg/l Al Chromazurole S
043 Aluminium Test* 114825 0.020 – 1.20 mg/l Al Chromazurole S
104 Ammonium Cell Test 114739 0.010 – 2.000 mg/l NH4-N Indophenol blue
051 Ammonium Cell Test 114558 0.20 – 8.00 mg/l NH4-N Indophenol blue
052 Ammonium Cell Test 114544 0.5 – 16.0 mg/l NH4-N Indophenol blue
053 Ammonium Cell Test 114559 4.0 – 80.0 mg/l NH4-N Indophenol blue
054 Ammonium Test 114752 0.010 – 3.00 mg/l NH4-N Indophenol blue
155 Ammonium Test 100683 2.0 – 75.0 mg/l NH4-N Indophenol blue
163 Ammonium Test 100683 5 – 150 mg/l NH4-N Indophenol blue
130 Antimony in water and wastewater Application 0.10 – 8.00 mg/l Sb Brilliant green
156 AOX Cell Test* 100675 0.05 – 2.50 mg/l AOX Oxidation to chloride
132 Arsenic Test* 101747 0.001 – 0.100 mg/l As Ag-DDTC
* turbidity correction possible
** individual calibration necessary

Release 06/2014
photoLab®S12
Available photometric test kits
Method Determination Total Range Method
No.
157 BOD Cell Test* 100687 0.5 – 3000 mg/l O2Modification of Winkler method
164 Boron Cell Test* 100826 0.05 – 2.00 mg/l B Azomethine H
046 Boron Test* 114839 0.050 – 0.800 mg/l B Rosocyanine
195 Bromate in water and drinking Application 0.003 – 0.120 mg/l BrO3 3,3’-Dimethylnaphtidine
water
146 Bromine Test* 100605 0.020 – 10.00 mg/l Br2S-DPD
067 Cadmium Cell Test 114834 0.025 – 1.000 mg/l Cd Cadion derivative
183 Cadmium Test 101745 0.0020 – 0.500 mg/l Cd Cadion derivative
165 Calcium Cell Test* 100858 10 – 250 mg/l Ca Phthalein purple
042 Calcium Test* 114815 5 – 160 mg/l Ca Glyoxal-bis-hydroxyanil
125 Calcium Test sensitive* 114815 1.0 – 15.0 mg/l Ca Glyoxal-bis-hydroxyanil
095 Chloride Cell Test* 114730 5 – 125 mg/l Cl Iron(III)-thiocyanat
110 Chloride Test* 114897 2.5 – 25.0 mg/l Cl Iron(III)-thiocyanat
063 Chloride Test* 114897 10 – 250 mg/l Cl Iron(III)-thiocyanat
218 Chloride Cell Test* 101804 0.5 – 15.0 mg/l Cl Iron(III)-thiocyanat
219 Chloride Test* 101807 0.10 – 5.00 mg/l Cl Iron(III)-thiocyanat
141 Chlorine Cell Test* (free chlorine) 100595 0.03 – 6.00 mg/l Cl2S-DPD
142 Chlorine Cell Test* 100597 0.03 – 6.00 mg/l Cl2S-DPD
(free and total chlorine)
143 Chlorine Test* (free chlorine) 100598 0.010 – 6.00 mg/l Cl2S-DPD
145 Chlorine Test* (total chlorine) 100602 0.010 – 6.00 mg/l Cl2S-DPD
144 Chlorine Test* 100599 0.010 – 6.00 mg/l Cl2S-DPD
(free and total chlorine)
194 Chlorine Cell Test*, Test* 100086/100087/ 0.010 – 6.00 mg/l Cl2DPD
(free and total chlorine) 100088
149 Chlorine dioxide Test* 100608 0.020 – 10.00 mg/l ClO2S-DPD
039 Chromate Cell Test* 114552 0.05 – 2.00 mg/l Cr Diphenylcarbazide
039 Chromate Cell Test* 114552 0.05 – 2.00 mg/l Cr Peroxodisulfate oxidation,
(total chromium) diphenylcarbazide
040 Chromate Test* 114758 0.010 – 3.00 mg/l Cr Diphenylcarbazide
020 Chromium Baths 4.0 – 400 g/l CrO3Inherent color
031 COD Cell Test* 114560 4.0 – 40.0 mg/l COD Chromosulfuric acid oxidation,
chromate determination
211 COD Cell Test* 101796 5.0 – 80.0 mg/l COD Chromosulfuric acid oxidation,
chromate determination
014 COD Cell Test* 114540 10 – 150 mg/l COD Chromosulfuric acid oxidation,
chromate determination
105 COD Cell Test* 114895 15 – 300 mg/l COD Chromosulfuric acid oxidation,
chromate determination
093 COD Cell Test* 114690 50 – 500 mg/l COD Chromosulfuric acid oxidation,
chromate determination
023 COD Cell Test* 114541 25 – 1500 mg/l COD Chromosulfuric acid oxidation,
chromium(III) determination
094 COD Cell Test* 114691 300 – 3500 mg/l COD Chromosulfuric acid oxidation,
chromium(III) determination
024 COD Cell Test* 114555 500 – 10000 mg/l COD Chromosulfuric acid oxidation,
chromium(III) determination
209 COD Cell Test* 101797 5000 – 90000 mg/l COD Chromosulfuric acid oxidation,
chromium(III) determination
137 COD Cell Test (Hg free)* 109772 10 – 150 mg/l COD Chromosulfuric acid oxidation,
chromate determination
138 COD Cell Test (Hg free)* 109773 100 – 1500 mg/l COD Chromosulfuric acid oxidation,
chromium(III) determination
220 COD Cell Test for seawater* 117058 5.0 – 60.0 mg/l COD Chloride depletion,
chromosulfuric acid oxidation,
chromate determination
221 COD Cell Test for seawater* 117059 50 – 3000 mg/l COD Chloride depletion,
chromosulfuric acid oxidation,
chromium(III) chromate determination
015 Color (445) CO445 0.1 – 50.0 m-1 Measurement at 445 nm
(spectral absorption coefficient)
061 Color (525) CO525 0.1 – 50.0 m-1 Measurement at 525 nm
(spectral absorption coefficient)
078 Color (620) CO620 0.1 – 250 m-1 Measurement at 620 nm
(spectral absorption coefficient)
032 Color Hazen* CU340 0.2 – 500 mg/l Pt/Co (Hazen) Platinum-cobalt-Standard Method,
measurement at 340 nm
179 Color Hazen* CU445 1 – 1000 mg/l Pt/Co (Hazen) Platinum-cobalt-Standard Method,
measurement at 445 nm
026 Copper Cell Test* 114553 0.05 – 8.00 mg/l Cu Cuprizone
027 Copper Test* 114767 0.02 – 6.00 mg/l Cu Cuprizone
* turbidity correction possible
** individual calibration necessary

Release 06/2014
photoLab®S12
Available photometric test kits
Method Determination Total Range Method
No.
083 Copper Baths 2.0 – 80.0 g/l Cu Inherent color
228 Cyanide Cell Test* 102531 0.010 – 0.500 mg/l CN Barbituric acid and
(free cyanide) pyridinecarboxylic acid
075 Cyanide Cell Test* 114561 0.010 – 0.500 mg/l CN Barbituric acid and
(free cyanide) pyridinecarboxylic acid
075 Cyanide Cell Test* 114561 0.010 – 0.500 mg/l CN Citronic acid, barbituric acid,
(readily liberated cyanide) and pyridinecarboxylic acid
109 Cyanide Test* 109701 0.0020 – 0.500 mg/l CN Barbituric acid and
(free cyanide) pyridinecarboxylic acid
109 Cyanide Test* 109701 0.0020 – 0.500 mg/l CN Citronic acid, barbituric acid,
(readily liberated cyanide) and pyridinecarboxylic acid
210 Cyanuric Acid Test 119253 2 – 160 mg/l CYA Triazine derivative
076 Fluoride Cell Test* 114557 0.10 – 1.50 mg/l F Alizarin complexone
124 Fluorid Cell Test sensitive 114557 0.025 – 0.500 mg/l F Alizarin complexone
215 Fluoride Cell Test* 100809 0.10 – 1.80 mg/l F Alizarin complexone
216 Fluorid Cell Test sensitive 100809 0.025 – 0.500 mg/l F Alizarin complexone
166 Fluorid Test* 114598 0.10 – 2.00 mg/l F Alizarin complexone
167 Fluorid Test* 114598 1.0 – 20.0 mg/l F Alizarin complexone
217 Fluorid Test 100822 0.02 – 2.00 mg/l F SPADNS
028 Formaldehyde Cell Test* 114500 0.10 – 8.00 mg/l HCHO Chromotropic acid
091 Formaldehyde Test* 114678 0.02 – 8.00 mg/l HCHO Chromotropic acid
045 Gold Test 114821 0.5 – 12.0 mg/l Au Rhodamine B
Hardness
see Total Hardness or Residual Hardness
Hazen
see Color Hazen
044 Hydrazine Test* 109711 0.005 – 2.00 mg/l N2H44-Dimethylaminobenzaldehyde
099 Hydrogenperoxide Cell Test* 114731 2.0 – 20.0 mg/l H2O2Titanyl sulfate
128 Hydrogenperoxide Cell Test sens.* 114731 0.25 – 5.00 mg/l H2O2 Titanyl sulfate
198 Hydrogenperoxide Test 118789 0.015 – 6.00 mg/l H2O2Phenanthroline derivative
147 Iodine Test* 100606 0.050 – 10.00 mg/l I2S-DPD
033 Iodine color number 0.010 – 3.00 Measurement at 340 nm
021 Iodine color number 0.2 – 50.0 Measurement at 445 nm
037 Iron Cell Test 114549 0.05 – 4.00 mg/l Fe Triazine
106 Iron Cell Test* 114896 1.0 – 50.0 mg/l Fe 2,2’-Dipyridyl
(Fe(II) and Fe(III))
038 Iron Test 114761 0.005 – 5.00 mg/l Fe Triazine
161 Iron Test* 100796 0.010 – 5.00 mg/l Fe 1,10-Phenanthroline
(Fe(II) and Fe(III))
066 Lead Cell Test* 114833 0.10 – 5.00 mg/l Pb PAR
160 Lead Test* 109717 0.010 – 5.00 mg/l Pb PAR
158 Magnesium Cell Test* 100815 5.0 – 75.0 mg/l Mg Phthalein purple
159 Manganese Cell Test* 100816 0.10 – 5.00 mg/l Mn Formaldoxime
184 Manganese Test* 101739 0.005 – 2.00 mg/l Mn PAN
019 Manganese Test* 114770 0.010 – 10.00 mg/l Mn Formaldoxime
226 Manganese Test* 101846 0.005 – 2.00 mg/l Mn PAN
135 Mercury in water and wastewater Application 0.025 – 1.000 mg/l Hg Michler’s ketone
175 Molybdenum Cell Test 100860 0.02 – 1.00 mg/l Mo Bromopyrogallol red
206 Molybdenum Test 119252 0.5 – 45.0 mg/l Mo Mercaptoacetic acid
185 Monochloramine Test 101632 0.050 – 10.00 mg/l Cl2Indophenol blue
017 Nickel Cell Test* 114554 0.10 – 6.00 mg/l Ni Dimethylglyoxime
018 Nickel Test* 114785 0.02 – 5.00 mg/l Ni Dimethylglyoxime
057 Nickel Baths 2.0 – 120 g/l Ni Inherent color
059 Nitrate Cell Test* 114542 0.5 – 18.0 mg/l NO3-N Nitrospectral
030 Nitrate Cell Test* 114563 0.5 – 25.0 mg/l NO3-N 2,6-Dimethylphenol
107 Nitrate Cell Test* 114764 1.0 – 50.0 mg/l NO3-N 2,6-Dimethylphenol
151 Nitrate Cell Test* 100614 23 – 225 mg/l NO3-N 2,6-Dimethylphenol
060 Nitrate Test* 114773 0.2 – 20.0 mg/l NO3-N Nitrospectral
139 Nitrate Test* 109713 0.10 – 25.0 mg/l NO3-N 2,6-Dimethylphenol
072 Nitrate Cell Test in seawater* 114556 0.10 – 3.00 mg/l NO3-N Resorcine
140 Nitrate Test in seawater* 114942 0.2 – 17.0 mg/l NO3-N Resorcine
227 Nitrate Test 101842 0.3 – 30.0 mg/l NO3-N Benzoic acid derivative
035 Nitrite Cell Test* 114547 0.010 – 0.700 mg/l NO2-N Griess reaction
197 Nitrite Cell Test* 100609 1.0 – 90.0 mg/l NO2-N Iron(II) ethylenediammonium sulfate
036 Nitrite Test* 114776 0.002 – 1.00 mg/l NO2-N Griess reaction
068 Nitrogen (total) Cell Test 114537 0.5 – 15.0 mg/l N Peroxodisulfate oxidation, nitrospectral
153 Nitrogen (total) Cell Test* 100613 0.5 – 15.0 mg/l N Peroxodisulfate oxidation,
2,6-dimethylphenol
108 Nitrogen (total) Cell Test 114763 10 – 150 mg/l N Peroxodisulfate oxidation,
2,6-dimethylphenol
092 Oxygen Cell Test* 114694 0.5 – 12.0 mg/l O2Modification of Winkler method
* turbidity correction possible
** individual calibration necessary

Release 06/2014
photoLab®S12
Available photometric test kits
Method Determination Total Range Method
No.
207 Oxygen Scavengers Test 119251 0.020 – 0.500 mg/l DEHA FerroZine®
148 Ozone Test* 100607 0.010 – 4.00 mg/l O3S-DPD
133 Palladium in water and wastewater Application 0.05 – 1.25 mg/l Pd Thio-Michler’s ketone
186 pH Cell Test 101744 6.4 – 8.8 Phenol red
073 Phenol Cell Test* 114551 0.10 – 2.50 mg/l Phenole MBTH
176 Phenol Test* 100856 0.025 – 5.00 mg/l C6H5OH Aminoantipyrine
177 Phenol Test* 100856 0.002 – 0.200 mg/l C6H5OH Aminoantipyrine, by extraction
212 Phosphate Cell Test 100474 0.05 – 5.00 mg/l PO4-P Phosphomolybdenum blue
055 Phosphate Cell Test 114543 0.05 – 5.00 mg/l PO4-P Phosphomolybdenum blue
055 Phosphate Cell Test 114543 0.05 – 5.00 mg/l P Peroxodisulfate oxidation,
(total phosphorus) phosphomolybdenum blue
213 Phosphate Cell Test 100475 0.5 – 25.0 mg/l PO4-P Phosphomolybdenum blue
086 Phosphate Cell Test 114729 0.5 – 25.0 mg/l PO4-P Phosphomolybdenum blue
086 Phosphate Cell Test 114729 0.5 – 25.0 mg/l P Peroxodisulfate oxidation,
(total phosphorus) phosphomolybdenum blue
152 Phosphate Cell Test 100616 3.0 – 100.0 mg/l PO4-P Phosphomolybdenum blue
214 Phosphate Cell Test 100673 3.0 – 100.0 mg/l PO4-P Phosphomolybdenum blue
214 Phosphate Cell Test 100673 3.0 – 100.0 mg/l P Peroxodisulfate oxidation,
(total phosphorus) phosphomolybdenum blue
056 Phosphate Test 114848 0.010 – 5.00 mg/l PO4-P Phosphomolybdenum blue
162 Phosphate Test 100798 1.0 – 100.0 mg/l PO4-P Phosphomolybdenum blue
069 Phosphate Cell Test* 114546 0.5 – 25.0 mg/l PO4-P Vanadatomolybdate
070 Phosphate Test* 114842 0.5 – 30.0 mg/l PO4-P Vanadatomolybdate
134 Platinium in water and wastewater Application 0.10 – 1.25 mg/l Pt o-Phenylendiamine
103 Potassium Cell Test 114562 5.0 – 50.0 mg/l K Kalignost, turbidimetric
150 Potassium Cell Test 100615 30 – 300 mg/l K Kalignost, turbidimetric
098 Residual Hardness Cell Test* 114683 0.50 – 5.00 mg/l Ca Phthalein purple
079 Silicate (Silicic acid) Test 114794 0.11 – 10.70 mg/l SiO2Silicomolybdenum blue
081 Silicate (Silicic acid) Test 114794 0.011 – 1.600 mg/l SiO2Silicomolybdenum blue
169 Silicate (Silicic acid) Test* 100857 1.1 – 107.0 mg/l SiO2Molybdatosilicate
171 Silicate (Silicic acid) Test* 100857 11 – 1070 mg/l SiO2Molybdatosilicate
225 Silicate (Silicic acid) Test 101813 0.0005 – 0.5000 mg/l SiO2Silicomolybdenum blue
047 Silver Test* 114831 0.25 – 3.00 mg/l Ag Eosine / 1,10-phenanthroline
168 Sodium Cell Test in nutrient 100885 10 – 300 mg/l Na indirectly as chloride
solutions*
229 Sulfate Cell Test 102532 1.0 – 50.0 mg/l SO4Bariumsulfate, turbidimetric
064 Sulfate Cell Test 114548 5 – 250 mg/l SO4Bariumsulfate, turbidimetric
154 Sulfate Cell Test 100617 50 – 500 mg/l SO4Bariumsulfate, turbidimetric
082 Sulfate Cell Test 114564 100 – 1000 mg/l SO4Bariumsulfate, turbidimetric
065 Sulfate Test* 114791 25 – 300 mg/l SO4Tannin
224 Sulfate Test 101812 0.50 – 50.0 mg/l SO4Bariumsulfate, turbidimetric
230 Sulfate Test 102537 5 – 300 mg/l SO4Bariumsulfate, turbidimetric
080 Sulfide Test* 114779 0.020 – 1.50 mg/l S Dimethyl-p-phenylendiamine
071 Sulfite Cell Test* 114394 1.0 – 20.0 mg/l SO3Ellman’s reagent
127 Sulfite Cell Test sensitive* 114394 0.05 – 3.00 mg/l SO3Ellman’s reagent
187 Sulfite Test* 101746 1.0 – 60.0 mg/l SO3Ellman’s reagent
087 Surfactants (anionic) Cell Test 114697 0.05 – 2.00 mg/l MBAS Methylene blue
(methylene blue active substances)
231 Surfactants (anionic) Cell Test 102552 0.05 – 2.00 mg/l MBAS Methylene blue
(methylene blue active substances)
192 Surfactants (cationic) Cell Test* 101764 0.05 – 1.50 mg/l k-Ten Disulfine blue
193 Surfactants (nonionic) Cell Test* 101787 0.10 – 7.50 mg/l n-Ten TBPE
182 Suspended Solids 25 – 750 mg/l SusS
100 Tin Cell Test* 114622 0.10 – 2.50 mg/l Sn Pyrocatechol violet
172 TOC Cell Test 114878 5.0 – 80.0 mg/l TOC Peroxodisulfate oxidation, indicator
173 TOC Cell Test 114879 50 – 800 mg/l TOC Peroxodisulfate oxidation, indicator
178 Total Hardness Cell Test* 100961 5 – 215 mg/l Ca Phthalein purple
Water hardness
see Total Hardness or Residual Hardness
077 Turbidity 1 – 100 FAU Measurement at 550 nm
191 Volatile Organic Acids Cell Test* 101763 50 – 3000 mg/l HOAc Esterification
222 Volatile Organic Acids Cell Test* 101749 50 – 3000 mg/l CH3COOH Esterification
223 Volatile Organic Acids Test* 101809 50 – 3000 mg/l CH3COOH Esterification
174 Zinc Cell Test 100861 0.025 – 1.000 mg/l Zn PAR
074 Zinc Cell Test 114566 0.20 – 5.00 mg/l Zn PAR
041 Zinc Test* 114832 0.05 – 2.50 mg/l Zn Cl-PAN
* turbidity correction possible
** individual calibration necessary

Measuring 0.20 – 8.00 mg/l NH4-N
range: 0.26 – 10.30 mg/l NH4
0.20 – 8.00 mg/l NH3-N
0.24 – 9.73 mg/l NH3
Expression of results also possible in mmol/l.
Pipette 1.0 ml of the
sample into a reaction
cell
, close with the screw
cap,
and mix.
Add 1 dose of NH4-1K
using the blue dose-
metering cap, close the
cell with the screw cap.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Reaction time:
15 minutes
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) we recommended
to use CombiCheck 10, Cat.No. 250482.
Ready-for-use ammonium standard solution, Cat.No.
250461,
concentration 1000 mg/l NH4
+, can also be used
after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 10) is highly recommended.
Important:
Very high ammonium concentrations in the sample
produce turquoise-colored solutions (measurement solu-
tion should be yellow-green to green) and false-low read-
ings are yielded. In such cases the sample must be di-
luted (plausibility check).
Check the pH of the
sample, specified range
:
pH 4 – 13.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
A6/25 · Ammonium
Release 06/2014 - photoLab®S12

14739 · Ammonium
Measuring 0.010 – 2.000 mg/l NH4-N
range: 0.01 – 2.58 mg/l NH4
0.010 – 2.000 mg/l NH3-N
0.01 – 2.43 mg/l NH3
Expression of results also possible in mmol/l.
Pipette 5.0 ml of the
sample into a reaction
cell
close with the screw
cap,
and mix.
Add 1 dose of NH4-1K
using the blue dose-
metering cap, close the
cell with the screw cap.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Reaction time:
15 minutes
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Check the pH of the
sample, specified range
:
pH 4 – 13.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) we recommended
to use CombiCheck 50, Cat.No. 250486.
Ready-for-use ammonium standard solution, Cat.No.
250461,
concentration 1000 mg/l NH4
+, can also be used
after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 50) is highly recommended.
Important:
Very high ammonium concentrations in the sample
produce turquoise-colored solutions (measurement solu-
tion should be yellow-green to green) and false-low read-
ings are yielded. In such cases the sample must be di-
luted (plausibility check).
Release 06/2014 - photoLab®S12

14544 · Ammonium
Measuring 0.5 – 16.0 mg/l NH4-N
range: 0.6 – 20.6 mg/l NH4
0.5 – 16.0 mg/l NH3-N
0.6 – 19.5 mg/l NH3
Expression of results also possible in mmol/l.
Pipette 0.50 ml of the
sample into a reaction
cell
close with the screw
cap,
and mix.
Add 1 dose of NH4-1K
using the blue dose-
metering cap, close the
cell with the screw cap.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Reaction time:
15 minutes
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Check the pH of the
sample, specified range
:
pH 4 – 13.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) we recommended
to use CombiCheck 20, Cat.No. 250483.
Ready-for-use ammonium standard solution, Cat.No.
250461,
concentration 1000 mg/l NH4
+, can also be used
after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 20) is highly recommended.
Important:
Very high ammonium concentrations in the sample
produce turquoise-colored solutions (measurement solu-
tion should be yellow-green to green) and false-low read-
ings are yielded. In such cases the sample must be di-
luted (plausibility check).
Release 06/2014 - photoLab®S12

14559 · Ammonium
Measuring 4.0 – 80.0 mg/l NH4-N
range: 5.2 – 103.0 mg/l NH4
4.0 – 80.0 mg/l NH3-N
4.9 – 97.3 mg/l NH3
Expression of results also possible in mmol/l.
Pipette 0.10 ml of the
sample into a reaction
cell
close with the screw
cap,
and mix.
Add 1 dose of NH4-1K
using the blue dose-
metering cap, close the
cell with the screw cap.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Reaction time:
15 minutes
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Check the pH of the
sample, specified range
:
pH 4 – 13.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) we recommended
to use CombiCheck 70, Cat.No. 250488.
Ready-for-use ammonium standard solution, Cat.No.
250461,
concentration 1000 mg/l NH4
+, can also be used
after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 70) is highly recommended.
Important:
Very high ammonium concentrations in the sample
produce turquoise-colored solutions (measurement solu-
tion should be yellow-green to green) and false-low read-
ings are yielded. In such cases the sample must be di-
luted (plausibility check).
Release 06/2014 - photoLab®S12

Measuring 5 –125 mg/l Cl
range: Expression of results also possible in mmol/l.
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) we recommended
to use CombiCheck 10 and 20, Cat.Nos. 250482 and
250483.
Ready-for-use chloride standard solution, Cat.No. 250466,
concentration 1000 mg/l Cl–, can also be used after dilut-
ing accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck) is highly recommended.
Check the pH of the
sample, specified range:
pH 1 – 12.
If required, add dilute
ammonia solution or
nitric acid drop by drop
to adjust the pH.
Pipette 0.50 ml of
Cl-1K into a reaction
cell, close with the screw
cap, and mix.
Add 1.0 ml of the sample
with pipette , close with
the screw cap, and mix.
Release 06/2014 - photoLab®S12
14730 · Chloride

14552 · Chromate
Determination of chromium(VI)
Measuring 0.05 – 2.00 mg/l Cr
range: 0.11 – 4.46 mg/l CrO4
Expression of results also possible in mmol/l.
Add 6 drops of Cr-3K
into a reaction cell, close
with the screw cap.
Shake the cell vigorous-
ly to dissolve the solid
substance and leave to
stand for 1 minute.
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) ready-for-use
chromate standard solution, Cat.No. 250468, concen-
tration 1000 mg/l CrO4
2–, can be used after diluting
accordingly.
Add 5.0 ml of the sample
with pipette , close the
cell with the screw cap,
and mix.
Reaction time:
1 minute
Check the pH of the
sample, specified range
:
pH 1 – 9.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Release 06/2014 - photoLab®S12

14552 · Chromate
Determination of total chromium (sum of chromium(VI) and chromium(III))
Measuring 0.05 – 2.00 mg/l Cr
range: 0.11 – 4.46 mg/l CrO4
Expression of results also possible in mmol/l and also in Cr total (Cr), Cr(III), and Cr(VI).
Add 6 drops of Cr-3K
into a reaction cell,
close the cell with the
screw cap.
Shake the cell vigorous-
ly to dissolve the solid
substance and leave to
stand for 1 minute.
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) ready-for-use
chromate standard solution, Cat.No. 250468, concen-
tration 1000 mg/l CrO4
2–, can be used after diluting
accordingly.
Add 5.0 ml of the pre-
treated sample
with
pipette
, close with the
screw cap, and mix.
Pipette 10 ml of the
sample into an empty
round cell (Empty cells,
Cat.No. 250621).
Add 1 drop of Cr-1K,
close with the screw cap,
and mix.
Add 1 dose of Cr-2K
using the blue dose-
metering cap, close the
reaction cell with the
screw cap.
Heat the cell in the
thermoreactor at 120 °C
(100 °C) for 1 hour.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool to room tempera-
ture:
pretreated sample .
A differentiation between chromium(VI) and
chromium(III) can be performed on the pho-
tometer. Prior to measuring, select the differ-
entiation measurement and choose the corre-
sponding citation form.Then measure the
total chromium, press enter and measure the
chromium(VI) (see analytical procedure for
chromium(VI)). After pressing enter, the
indi-
vidual measuring values for Cr VI and Cr III
are
shown on the display.
Reaction time:
1 minute
Check the pH of the
sample, specified range
:
pH 1 – 9.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Release 06/2014 - photoLab®S12

Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Carefully pipette 3.0 ml
of the sample into a
reaction cell, close tight-
ly with the screw cap,
and mix vigorously.
Caution, the cell
becomes hot!
Suspend the bottom
sediment in the cell by
swirling.
Heat the reaction cell in
the thermoreactor at
148 °C for 2 hours.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool.
Swirl the cell after
10 minutes.
Replace the cell in the
rack for complete cooling
to room temperature.
Very important !
Measuring 10 –150 mg/l COD or O2
range: Expression of results also possible in mmol/l.
C3/25 · Chemical Oxygen Demand
Release 06/2014 - photoLab®S12
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) we recommended
to use CombiCheck 10, Cat.No. 250482.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 10) is highly recommended.

Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Carefully pipette 3.0 ml
of the sample into a
reaction cell, close tight-
ly with the screw cap,
and mix vigorously.
Caution, the cell
becomes hot!
Suspend the bottom
sediment in the cell by
swirling.
Heat the reaction cell in
the thermoreactor at
148 °C for 2 hours.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool.
Swirl the cell after
10 minutes.
Replace the cell in the
rack for complete cooling
to room temperature.
Very important !
Measuring 4.0 –40.0 mg/l COD or O2
range: Expression of results also possible in mmol/l.
14560 · Chemical Oxygen Demand
Release 06/2014 - photoLab®S12
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) we recommended
to use CombiCheck 50, Cat.No. 250486.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 50) is highly recommended.

Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Carefully pipette 2.0 ml
of the sample into a
reaction cell, close tight-
ly with the screw cap,
and mix vigorously.
Caution, the cell
becomes hot!
Suspend the bottom
sediment in the cell by
swirling.
Heat the reaction cell in
the thermoreactor at
148 °C for 2 hours.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool.
Swirl the cell after
10 minutes.
Replace the cell in the
rack for complete cooling
to room temperature.
Very important !
Measuring 15–300 mg/l COD or O2
range: Expression of results also possible in mmol/l.
14895 · Chemical Oxygen Demand
Release 06/2014 - photoLab®S12
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) we recommended
to use CombiCheck 60, Cat.No. 250487.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 60) is highly recommended.

Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Carefully pipette 2.0 ml
of the sample into a
reaction cell, close tight-
ly with the screw cap,
and mix vigorously.
Caution, the cell
becomes hot!
Suspend the bottom
sediment in the cell by
swirling.
Heat the reaction cell in
the thermoreactor at
148 °C for 2 hours.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool.
Swirl the cell after
10 minutes.
Replace the cell in the
rack for complete cooling
to room temperature.
Very important !
Measuring 50 –500 mg/l COD or O2
range: Expression of results also possible in mmol/l.
14690 · Chemical Oxygen Demand
Release 06/2014 - photoLab®S12
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) we recommended
to use CombiCheck 60, Cat.No. 250487.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 60) is highly recommended.

Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Carefully pipette 3.0 ml
of the sample into a
reaction cell, close tight-
ly with the screw cap,
and mix vigorously.
Caution, the cell
becomes hot!
Suspend the bottom
sediment in the cell by
swirling.
Heat the reaction cell in
the thermoreactor at
148 °C for 2 hours.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool.
Swirl the cell after
10 minutes.
Replace the cell in the
rack for complete cooling
to room temperature.
Very important !
Measuring 25–1500 mg/l COD or O2
range: Expression of results also possible in mmol/l.
C4/25 · Chemical Oxygen Demand
Release 06/2014 - photoLab®S12
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) we recommended
to use CombiCheck 20, Cat.No. 250483.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 20) is highly recommended.

Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Carefully pipette 2.0 ml
of the sample into a
reaction cell, close tight-
ly with the screw cap,
and mix vigorously.
Caution, the cell
becomes hot!
Suspend the bottom
sediment in the cell by
swirling.
Heat the reaction cell in
the thermoreactor at
148 °C for 2 hours.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool.
Swirl the cell after
10 minutes.
Replace the cell in the
rack for complete cooling
to room temperature.
Very important !
Measuring 300–3500 mg/l COD or O2
range: Expression of results also possible in mmol/l.
14691 · Chemical Oxygen Demand
Release 06/2014 - photoLab®S12
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) we recommended
to use CombiCheck 80, Cat.No. 250489.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 80) is highly recommended.

Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Carefully pipette 1.0 ml
of the sample into a
reaction cell, close tight-
ly with the screw cap,
and mix vigorously.
Caution, the cell
becomes hot!
Suspend the bottom
sediment in the cell by
swirling.
Heat the reaction cell in
the thermoreactor at
148 °C for 2 hours.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool.
Swirl the cell after
10 minutes.
Replace the cell in the
rack for complete cooling
to room temperature.
Very important !
Measuring 500 –10000 mg/l COD or O2
range: Expression of results also possible in mmol/l.
14555 · Chemical Oxygen Demand
Release 06/2014 - photoLab®S12
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) we recommended
to use CombiCheck 70, Cat.No. 250488.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 70) is highly recommended.