YOKOGAWA CQ1 User manual

User’s
Manual
Confocal Quantitative Image Cytometer
IM 80J01A01-01E
The translation of the original instructions
~However warning labels are not the same.~
IM 80J01A01-01E
Yokogawa Electric Corporation 10th Edition

This document corresponds to the following versions of
software
Measurement software R1.04

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Introduction
Thank you for purchasing the Confocal Quantitative Image Cytometer
CQ1.
This manual describes the functions, installation and wiring procedures,
operating procedures and handling precautions of the CQ1. Before you
start to use the CQ1, please read this manual carefully to enable
correct use of the system. After reading this manual, keep it in a handy
place so that you can refer to it whenever necessary.
Notes
The information contained in this manual is subject to change
without prior notice due to improvements in performance and
functionality or for other reasons.
This manual has been prepared with the utmost care; however, if
you have any questions, or note any errors, please contact our
branch or sales office of the neighborhood.
Unauthorized reproduction or reprinting of this manual in whole or
in part is prohibited.
Follow the operating instructions to keep stable system
operation. .
Trademarks
Microsoft and Windows are registered trademarks or trademarks
of Microsoft Corporation in the United States and other countries.
In this document, registered trademarks or trademarks are not
indicated by ™ and ®.
History June 2014: 1st edition issued
October 2014: 2nd edition issued
March 2015: 3rd edition issued
March 2015: 4th edition issued
April 2015: 5th edition issued
October 2015: 6th edition issued
June 2016: 7th edition issued
June 2016: 8th edition issued
July 2016: 9th edition issued
April 2017:10th edition issued

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For Safe Use of This Equipment
To ensure safe and correct use of this equipment, be sure to follow the
precautions below when using this equipment. If this equipment is used in
a manner not specified in this manual, the protection provided by this
equipment may be impaired. If you do not follow the precautions when
using the equipment, we assume no responsibility nor provide any
guarantee regarding its safety and function.
Following symbols are used in the manual in the following cases.
WARNING
This symbol describes precautions to avoid hazardous situations that
could result in a bodily injury or death of the user upon mishandling
and/or incorrect use of the equipment.
CAUTION
This symbol describes precautions to avoid hazardous situations that
could result in a minor bodily injury of the user, or that could result in
damage to the equipment upon mishandling and/or incorrect use of the
equipment.
POWER ON
This symbol indicates POWER ON.
POWER OFF
This symbol indicates POWER OFF.

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Following warning labels are attached on this equipment.
Warning label (1)
This warning label indicates that the operator
must refer to the instruction manual to prevent
injury and/or damages on the instrument.
Warning label (2)
This warning label indicates that this is laser
product and operator must prevent injury and/or
damages of skin or eyes from laser exposure.
Warning label (3)
This label warns the users on dangers that
require safety control measures when using the
laser. To be specific, the users are cautioned
that opening this cover results in emission of
visible and invisible Class 3B laser beam and
that entry of this beam into the eyes or its
contact with the skin is a potential health
hazard.
Warning label (4)
This label warns the users on dangers that
require safety control measures when using the
laser. To be specific, the users are cautioned
that opening this cover results in emission of
visible and invisible Class 3B laser beam and
that entry of this beam into the eyes or its
contact with the skin is a potential health
hazard.
Aperture label
Warning sign to indicate the laser aperture.
Explanation label (1)
This is a Class 1 laser product.
Explanation label (2)
Warning sign to indicate the wavelength,
maximum laser output power and laser class.
The Utility Box belongs to the Class 3B laser
product.

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Location of Warning Labels
󳔤Right Side of Main Unit
󳔤Rear Panel of Main Unit
󳔤Top Panel of Main Unit
Warning label (3)
Explanation label (1)
Warning label (4)
Warning label (3)
Warning label (3)

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󳔤Rear Panel of Main Unit (Transmission Illumination Option)
󳔤Front Panel of Utility Box
󳔤Rear Panel of Utility Box
Warning label (3)
Warning label (3)
Warning label (1)
Warning label (1)
Warning label (2)
Explanation label (2)

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󳔤Right Side of Utility Box
Aperture label

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Observe the following precautions to prevent hazardous situations that
could result in a bodily injury or death of the user, such as electric
shock, or that could result in damage to the equipment.
WARNING
Be sure to turn on the power to the equipment after confirming that
the power-supply voltage of the equipment matches the voltage of
the supplied power. Always use the dedicated power cable
supplied with your equipment.
This is a Class 1 laser product. However, the equipment houses a
Class 3B laser, which is protected by the enclosure and the
interlocks provided at openings. When using this product, heed the
precautions explained in 󳆟Laser Products Handling Precautions.󳆠
Controls and adjustments other than according to the procedures
specified herein will result in dangerous exposure to laser.
If you use Gas Mixer, CQ1 emits max. 20 % CO2mixture gas at a
rate of 200ml/min. If CO2concentration in the air exceeds 3%, it
may be harmful to the human body. Be sure to provide ventilation,
lead exhaust gas from CQ1 to outside of the room, or take other
appropriate measures. It is also recommended that an alarm
system be installed in case of emergency.
If you use Gas Mixer, CQ1 emits min. 0.1% low O2gas at a rate of
200ml/min. If O2concentration in the air drops below 18%, it may be
harmful to the human body. Be sure to provide ventilation, lead
exhaust gas from CQ1 to outside of the room, or take other
appropriate measures. It is also recommended that an alarm system
be installed in case of emergency.
Do not put a container with liquid or powder on the Main Unit,
Utility Boxes or cables of this instrument. Also, do not spill liquid or
powder on these materials. These materials may cause electric
shock and suffer electrical or mechanical damages.
Do not use the equipment by wet hand. It may cause electric
shock and suffer electrical or mechanical damages.

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CAUTION
Use the I/O terminals of the equipment within the ranges of the
specifications to prevent damage to the equipment.
This product uses precision optics. Therefore, do not use the
equipment in locations where there are large vibrations, a lot of
dust, high humidity and high temperature (in places near heating
equipment or exposed to direct sunlight), rapid changes in the
temperature (in places subject to dew condensation), or corrosive
or combustible gases.
Never touch any of the internal parts of this product. And never
take apart or modify this product. The optical system housed in the
product may become dirty, damaged or out of calibration and so
on, leading to equipment failure.
If the equipment has malfunctions or damages, please
immediately cease use and contact us.
This product is designed with the intention to use in industrial
environment. If this product is used in residential environment, it
may have influences to other equipments..

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Laser Products Handling Precautions
Laser products are classified according to their exposure emission
limit determined by the wavelength and power characteristics of
laser beam. A different set of common safety standards applies to
each class of products. The product explained herein belongs to
Class 1. However, the equipment houses a Class 3B laser, which
is protected by the enclosure and the interlocks provided at
openings.
Only service personnel can remove the covers on which a warning
label is attached. If these covers are removed, Class 3B laser
beam will be emitted. Directly looking into Class 3B laser beam or
beam reflected on a mirror may cause eye damage and is
extremely dangerous. Exercise due caution when handling this
product.
(Reference)
In the IEC standard, the laser classes are briefly explained as follows:
Class 1󳞷Essentially safe. Continue to look into the Class 1 laser will not cause any
danger.
Class 2󳞷If the Class 2 laser is looked into for a period not more than 0.25 second, there is
no risk of danger.
Class 3B󳞷Looking into the laser beam from this class of equipment is dangerous. Even if
the specification of the laser equipment used does not exceed this class, there
is still a risk of eye injury.
Safety Standards
A class 1 laser is safe under all conditions of normal use. This
means the maximum permissible exposure can not be exceeded.
This equipment is designed in accordance with the IEC60825-1
Radiation safety standards for laser products and these lasers
must be labeled with the following label, but are exempt from the
requirements of the Laser Safety Program.
Preventive Measures on Safety
Mirror reflection
Metals and other glossy reflective surfaces reflect the laser beam.
Exercise caution to prevent unexpected mirror reflection.
Beam path
Never look directly into the laser beam or touch the laser beam.
CLASS 1 LASER PRODUCT

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WEEE󳞥Waste Electrical and Electronic Equipment󳞦,
Directive
󳞥This directive is only valid in the EU.󳞦
This instrument complies with the WEEE Directive marking
requirement. The marking above indicates that you must not
discard this instrument in domestic household waste.
Product Category
With reference to the instrument type in the WEEE directive Annex
1, this product is classified as a “Monitoring and Control instrument”
product.
When disposing this instrument in the EU, contact the distributer
whom you bought it from. Do not dispose in domestic household
waste.
Indemnity
Yokogawa shall provide no warranty regarding this product, unless
otherwise specified separately in the “Warranty Rule.”
Yokogawa shall assume no responsibility for any loss suffered by
a customer or third party as a result of use of this product, or any
loss or indirect loss suffered by a customer or third party due to a
defect in this product or any other problem not predictable by
Yokogawa.

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Checking the Content of the Package
After opening the package box, check if the following items are
included before beginning to use of this product. Should you find that a
wrong product has been delivered or any of the items is missing or if
you notice any abnormality on the exterior, please contact the dealer
from which you purchased your product.
CQ1
Part
Quantity
Description
CQ1 Main Unit
1
Confocal fluorescent microscope
system to observe cells cultured in
vessels such as Microplate or slide
glass
USB cable
1
Communication cable to connect
CQ1 main unit and workstation
Main Unit󳊞UTB cable
1
Power supply and communication
cable to connect CQ1 main unit
and utility box
Utility Box
1
Equipment for supply power and
excitation laser light
Optical Fiber
1
Optical fiber to guide laser from
utility box into main unit
AC Cord (CQ1)
1
AC cord for utility box
(Supplied by dealer)

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CQ1 (Continue from previous page)
Part
Quantity
Description
Key
1
Key switch on utility box
Tie-wrap
1
Tie-wrap for bundle USB cable and
Main Unit󳊞UTB cable
Remote Interlock
1
Remote interlock connector on
Utility Box
Objective Lens
Part
Quantity
Description
Objective lens
1󳟻6
To be installed on CQ1 main unit
One to six lenses are installable
depending on the specification.
Please refer to Parts List:
CMPL 80J01A01-01 for details.
Workstation
Part
Quantity
Description
Workstation
1
Attached with mouse, key board,
back-up CD and instruction
manual.
AC Cord (WS)
1
AC cord for workstation.
(Supplied by dealer)

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Option (Sold separately)
Part
Quantity
Description
Monitor
1
(Supplied by dealer)
AC Cord (Monitor)
1
(Supplied by dealer)
Monitor󳊞WS cable
1
(Supplied by dealer)
Transmission Illumination
1
Transmitted light for phase
contrast/ bright field observation
There are following 2 types
- Bright field/ Phase contrast
switchable type (manual)
- Phase contrast type
Gas mixer
(TOKAI HIT, GM-8000)
1
Supply mixture gas that O2and
CO2concentration is contolled to
stage attachment
Gas mixer
connecting tube
1
Tube to connect CQ1 main unit and
Gas mixer
Attached if stage heater option is
selected
Stage attachment
(All wells imaging type)
1
Stage attachment that all wells of
microplate can be imaged

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Option (Sold separately, continue from
previous page)
Part
Quantity
Description
Stage attachment
(Chambered type)
1
Stage attachment which can keep
the temperature, O2CO2
concentration and humidity
constant
Sample holder for
triple 35 mm dishes
1
Sample holder for set three 35 mm
dishes
Sample holder for
single 60 mm dish
1
Sample holder for set one 60 mm
dish
Sample holder for
slide glass (ISO 8037/1)
1
Sample holder for set four slide
glasses (ISO 8037/1)
Sample holder for
cover glass chamber
1
Sample holder for set one cover
glass chamber
60 mm dish ring
1
Hold silicone rubber when using
chambered type stage attachment
and 60 mm dish
Rubber for 60 mm dish
1
Set on 60 mm dish when using
chambered type stage attachment
Holding plate for CGC
1
Hold silicone rubber when using
chambered type stage attachment
and cover glass chamber
Rubber for CGC
1 - 4
Set on cover glass chamber when
using chambered type stage
attachment
Shape is different by type of cover
glass chamber

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Sample holders are set into the stage attachment as shown below.
Sample
Sample holder
Stage attachment
XY stage

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How to use this manual
This user manual consists of Chapters 1 to 9, the details of which are
explained below.
Chapter
Title and Content
1
Function Overview
A functional overview of this equipment is explained.
2
Before Use
Precautions for use and installation/wiring methods are explained.
3
Name of Each Part/Starting and Shutting Down the Equipment
The name of each part and starting/shutdown of the equipment are explained.
4
Measurement procedures
Procedures for measurement are explained
5
Functions of measurement software
Screen of measurement software is explained
6
Maintenance
Maintenance parts and consumable parts are explained.
7
Troubleshooting
Error messages and troubleshooting procedures for this equipment are explained.
8
Specifications
Specifications of this equipment are described.
9
Warranty Rule
The rules of warranty applicable to this equipment are explained.

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Content
1Function Overview ..........................................1-1
1.1 About the CQ1 ........................................................................... 1-1
1.2 Principle of the Nipkow Disk System with Micro-lens............. 1-3
2Precautions for Use/Installation.................2-1
2.1 Precautions for Use ................................................................................................... 2-1
2.2 Installing the Equipment........................................................................................ 2-2
2.3 Wiring the Peripherals.............................................................................................. 2-6
2.4 Connecting the Power ............................................................................................. 2-7
3Name of Each Part/Starting and
Shutting Down the Equipment....................3-1
3.1 Name and Function of Each Part..................................................................... 3-1
3.2 Starting and Shutting Down of CQ1............................................................... 3-8
3.3 Starting and Shutting Down of Gas Mixer (Option) .........................3-13
4Measurement Procedures.............................4-1
4.1 Measurement Procedures ..................................................................................... 4-1
4.2 Basic Protocol Setting...........................................................................................4-31
4.3 Sample Searching .....................................................................................................4-33
4.4 Imaging Setting...........................................................................................................4-36
4.5 Analysis Protocol Setting....................................................................................4-39
4.6 Object Recognizing Condition Setting ......................................................4-42
4.7 Protocol Saving...........................................................................................................4-43
4.8 Sample Measurement.............................................................................................4-45

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5Functions of CQ1 Software ..........................5-1
5.1 Outline.................................................................................................................................. 5-1
5.2 Main Menu ......................................................................................................................... 5-3
5.3 Operation Mode Panel.............................................................................................. 5-8
5.4 Control Panel (CQ Mode)...................................................................................... 5-10
5.5 Control Panel (HDD Mode) ..................................................................................5-56
5.6 Viewer ................................................................................................................................5-80
5.7 Status Panel ................................................................................................................5-122
5.8 Viewer Layout ............................................................................................................5-123
5.9 Channel Setting.........................................................................................................5-124
5.10 Trimming Parameter .............................................................................................5-126
5.11 User Setting.................................................................................................................5-127
5.12 Activation Setting ...................................................................................................5-128
5.13 Advanced Editor .......................................................................................................5-129
6Maintenance......................................................6-1
6.1 Maintenance of Workstation............................................................................... 6-1
7Trouble Shooting .............................................7-1
7.1 Trouble Shooting during Measurement Preperation......................... 7-1
7.2 Trouble Shooting during Imaging .................................................................... 7-4
7.3 Trouble Shooting during Analyzing ..............................................................7-15
7.4 Other Trouble Shootings......................................................................................7-17
8Specifications...................................................8-1
8.1 Details of MS codes ................................................................................................... 8-1
8.2 General Specifications............................................................................................ 8-4
8.3 Measurement sample vessels ........................................................................... 8-5
8.4 Applicable Standards ............................................................................................... 8-6
9Warranty Rule ...................................................9-1