YSI 2730 User manual

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Find the YSI 2700D at our website: Click HERE

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Monitor and Control
Accessory
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Table of Contents
1. Introduction ................................................................................................................................................ 1
1.1 Product Description ............................................................................................................................. 1
1.2 Features................................................................................................................................................. 1
1.3 Product Specifications .......................................................................................................................... 2
1.4 Digital Signals....................................................................................................................................... 3
2. Installation .................................................................................................................................................. 4
2.1 Unpacking............................................................................................................................................. 4
2.2 Installation ............................................................................................................................................ 4
3. Configuration.............................................................................................................................................. 9
3.1 Monitor ................................................................................................................................................. 9
3.2 Analog/Control ..................................................................................................................................... 9
3.2.1 Full Scale Voltage........................................................................................................................9
3.2.2 Full Scale Concentration............................................................................................................ 10
3.2.3 Discrete Output Polarity ............................................................................................................ 10
4. Interfacing................................................................................................................................................. 10
4.1 Sample Interface................................................................................................................................. 10
4.1.1 Filtrate Pump ............................................................................................................................. 10
4.1.2 Examples.................................................................................................................................... 11
4.2 Electrical Interface.............................................................................................................................. 12
4.2.1 Analog Outputs.......................................................................................................................... 12
4.2.2 Pump Control Outputs............................................................................................................... 12
4.2.3 Auxiliary Connector / Signal List.............................................................................................. 13
5. Basic Operation.........................................................................................................................................13
5.1 Software.............................................................................................................................................. 14
5.2 Setup ................................................................................................................................................... 15
5.2.1 Monitor...................................................................................................................................... 15
5.2.2 Antiseptic................................................................................................................................... 15
5.2.3 Sterilization................................................................................................................................ 16
5.2.4 Make-Up Cycle.......................................................................................................................... 16
5.2.5 Autocalibration .......................................................................................................................... 16
5.2.6 Analog /Control......................................................................................................................... 17
5.3 Service ................................................................................................................................................ 20
5.3.1 Monitor...................................................................................................................................... 20
5.3.2 Analog /Control......................................................................................................................... 20
6. PID Control...............................................................................................................................................20
6.1.1 Assumptions .............................................................................................................................. 20
7. Printed Setup Information.........................................................................................................................21
8. Service ...................................................................................................................................................... 22
8.1 Tubing Replacement........................................................................................................................... 22
8.1.1 Pump Tubing Replacement........................................................................................................ 22
8.1.2 Solenoid Valve Tubing Replacement ........................................................................................23
8.2 External Chamber............................................................................................................................... 23
8.3 Troubleshooting.................................................................................................................................. 24
8.4 Schematic Diagram............................................................................................................................. 27
8.5 Parts List............................................................................................................................................. 29
8.5.1 Monitor Pump/Solenoid Assembly............................................................................................29
8.5.2 External Chamber Assembly..................................................................................................... 31
9. Appendix A - Required Notice................................................................................................................. 32
10. Appendix B - Warranty and Shipping Information ................................................................................ 33
10.1 Cleaning Instructions........................................................................................................................ 34
10.2 Packing Instructions.......................................................................................................................... 35
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List of Figures
Figure 1 PC Board Installation ...................................................................................................................... 4
Figure 2 Eprom Installation...........................................................................................................................5
Figure 3 Fuse Installation .............................................................................................................................. 6
Figure 4 Pump Installation............................................................................................................................. 7
Figure 5 Tube Routing Label......................................................................................................................... 8
Figure 6 JP1 Settings .....................................................................................................................................9
Figure 7 Output Polarity .............................................................................................................................. 10
Figure 8 Handshake Signals ........................................................................................................................12
Figure 9 Software Flow Chart...................................................................................................................... 14
Figure 10 Minimum Purge Time .................................................................................................................15
Figure 11 Installing Pump Tubing............................................................................................................... 22
Figure 12 Schematic 1 .................................................................................................................................27
Figure 13 Schematic 2 .................................................................................................................................28
Figure 14 Monitor Pump/Solenoid Assembly ............................................................................................. 30
Figure 15 External Chamber Assembly.......................................................................................................31
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The YSI 2730 Monitor and Control Accessory allows “on-line” monitoring and control of sterile systems
over long periods of time without contamination using the 2700 SELECT. It also provides an alternative
means of interfacing the YSI 2700 SELECT biochemistry analyzer with external measurement/control
systems. The product consists of the monitor (purge) pump, solenoid valve assembly, external sample
chamber, circuit board, new instrument EPROM firmware, interface cable and a users manual.
When installed on the YSI 2700 SELECT, the monitor pump may draw sample from a process stream,
bioreactor or other suitable source and deliver sample to the external chamber of the instrument. Once
delivered to the external chamber, the 2700 SELECT Sipper Tube rotates out to the chamber to aspirate a
preprogrammed volume of sample. The 2700/2730 system can operate unattended for days or weeks,
provided sufficient reagent supply is considered.
The Monitor Pump is a dual channel style pump, thus excess sample (waste) is actively pumped from the
external sample chamber while fresh sample is being delivered. This strategy helps to minimize the dead
volume in the chamber while controlling excess sample removal. The sample volume required for each
analysis varies somewhat depending on the distance, flow rate and fluid interface used, however, typically
1.5 milliliters is sufficient to purge the external sample chamber and deliver fresh sample. The flow rate of
the pump and the purge time can be varied and is programmed via the 2700 software.
The Solenoid Valve assembly allows multiplexing between the sample stream and an antiseptic stream.
This preserves sterility by filling the end of the sampling line with an antiseptic after every sample.
The analog output section of the product creates a voltage signal which is proportional to the concentration
of the analyte. The YSI 2730 provides this voltage output for up to two chemistries, two "handshake"
signals and a system status signal. In addition, the user has the ability to adjust full scale for each
chemistry, in discrete steps, to accommodate 1, 2, 3, or 4 times the calibrator concentration. The YSI 2730
also provides three discrete signal outputs (TTL logic level) to control external pumps which can be used
to replenish nutrients or optimize byproduct concentrations.
The installation of the YSI 2730 accessory precludes turntable (YSI 2710) operation. The YSI 2730 uses
the Auxiliary output connector on the back of the YSI 2700 instrument.
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Monitor
•Prevents host contamination for at least six months when antiseptic system is properly used
•Retrofittable to all 2700’s with software version 2.41 or higher
•User programmable sample flow rate
•User programmable purge time
•User-selected anti-septic cycle
•Autoclavable pump and tubing
•Allows analysis of discrete samples without affecting monitoring
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Analog/Control
•Dual selectable analog outputs
•PID control
•Normal or inverted TTL outputs
•Allows analysis of discrete samples without initiating regulation
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Monitor
Size:
External Chamber............................................. 0.75 x 0.75 x 0.88 inches
Pump Head....................................................... 2.0 x 2.2 x 0.85 inches
Pump Motor......................................................... 13.6 VDC, 2-40 rpm, 22 inch-ounce torque
(power source is 2730 Board)
Sample Inlet Tubing ............................................ Silicone, 0.08 OD x 0.02 ID (inches)
Volume............................................................. 5.1 microliters/inch
Inlet Channel Pump Tubing................................. PharMed, 0.13 OD x 0.035 ID (inches)
Valve Tubing....................................................... 0.03 ID (inches)
Wasteline Tubing................................................. Silicone, 0.16 OD x 0.10 ID (inches)
Nominal Flow Rate (inlet line)............................ 100 - 2500 microliters/minute (±8% @ ±6 PSI)
Analog/Control
Full Scale Voltage................................................ Selectable: +10.00 VDC or +5.00 VDC
Full Scale Concentration...................................... User selectable via software as 1, 2, 3 or 4 x Calibrant
Concentration.
Resolution............................................................ 1:4096 or 0.02% FS,
2.44 mv on +10.00 VFS,
1.22 mv on +5.00 VFS
Maximum Offset.................................................. ±4 LSB
Linearity............................................................... ±1 LSB
Minimum analog output Load Impedance........... 2K Ohms
Logic output drive................................................ 0 and 5 VDC nominal at 4 milliamps
Logic Input levels................................................ < 0.8 VDC = logic 0,
> 3.5 VDC = logic 1
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Signal Direction Description
Ready output Active High, set immediately after analog outputs have
been updated. This signal will reset itself immediately
prior to updating the analog outputs if not reset externally
via the Ack\ signal input.
Ack\ input Active low, resets Ready output, minimum width >10ms.
SysErr output Active High, indicates 2700 not ready to sample.
Filtrate output external sample pump control.
BLK. Pump output feed/diluent pump control for Black Probe Chemistry.
WHT. Pump output feed/diluent pump control for White Probe Chemistry.
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Remove the parts from the internal shipping container. Be careful not to discard any parts or supplies.
Check off all items on the Packing List and inspect all assemblies and components for damage. In the
event of damaged or missing parts, contact YSI Technical Support or your Dealer Representative
immediately. Refer to APPENDIX B for phone, fax and address information.
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The YSI 2730 Monitor and Control accessory requires a YSI 2700 SELECT biochemistry analyzer
installed with software version 2.41 or higher for purposes of compatibility. To install the accessory a
small flat-head screwdriver, a #2 Phillips screwdriver, needle-nose pliers and a 5/16″or small adjustable
wrench are needed. The steps are as follows:
1. Turn off instrument power using the switch on the rear of the 2700 case. Do not unplug the power
cord.
2. Remove the two screws on the upper rear of the instrument to allow the hinged back panel to open.
Open the rear panel to expose the main printed circuit board and the smaller "piggyback" YSI 2710
turntable controller board as shown in Figure 1.
3. Discharge any static electricity from your body by touching the YSI 2700 chassis momentarily.
Figure 1 PC Board Installation
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4. Remove the turntable controller board by disconnecting the two cables which plug into it. Disconnect
the cables at the controller board itself, then remove the two mounting screws that hold the board to
the standoffs (hold the standoffs with a wrench or pliers if necessary). Remove the board and place it
aside for a moment.
5. Refer to Figure 2 to help you find the location of the 28 pin EPROM IC U11. Note the orientation of
the IC, specifically the position of the "notch" at the bottom of the part near the battery holder.
6. Discharge any static electricity again by touching the YSI 2700 chassis momentarily.
7. Remove the IC U11 from its socket using a small flat-head screw-driver or pocket knife. Make sure to
place the screwdriver under the IC and not the socket before you start to pry it up. Avoid bending the
pins. Best results are obtained by alternately prying one end of the IC and then the other.
8. Once the old EPROM has been removed, make sure that all the pins are straight on the new 2730
EPROM and install it in the U11 socket. Make sure that the orientation of the IC (the position of the
notch) agrees with that of the part that was just removed. Once all the pins are aligned, gently press it
into its socket.
1
J13
R76
X2
R77
C16
C2
R78
C40
C19
C32
SW1
R55
R52
D24
R49
D27
D29 D28 D26
C81
U31
C80
U2
U3
U35
U33 U34
C64
C65
J15
LED
D14
D13
1
J9
C29
U16
U19
R27
R32 R33
R26
Q1
Q6
Q5
Q4
U25
D15
D19
D20
D17
D18
D16
D9
D12 D11
D10
1
J8
1
J12
1
J11
U24
U23
U21
U22
U4 U15 U20 U26
U32
C77C78C79
U17
U28
C88
C38
C76
C83
C85C84
C82
C75
C37
C31 C43
C49
C62
C56
C63
C59
C60
C67 C66
C89
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C27
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C6
C5
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C50
C44
C36
Q3
C28
C20
R15
C24C23
C57
C55
R75
C30
C35
D21
C39
C74
D31
D32
D30
U18
U27
U29
TC3
TC2
J4
D33
U10
U9
C70
R61
JB2 JB1 JA1
R17
U14
RN1
RN3 RN4
RN5
RN7
RN6
RN2
D5 D4
D3
D2 D1
D8
D6
D7
R71
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F7
1
J7
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1
J1
1
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R57
R1
R36
R43
U1
R8
R64
R2
C33
F2
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R20
R4
R14
R16
U6
R54
R7
R6
R23
R29
R35
R25
R28
R34
R24
R39
R38 R37
F3
F4
F6
R30
R31
U5
R69
R59
R58
R68
R66 R56
R21
R19
R72
R74
R67
R63
R73
U7
R62
R65
R45
R44
R50
R3
R47
R53
R48
U12
C3
C4
C42
C48
C47
C46
C45
C51
C52 C54
C53
C25
C26
C71
C8
C10 C9
C7
C11
C13
C14
C12
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C72
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F1
C69
F5
U11
U13
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+
A1
U36
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R81
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Q7 Q8
C1
U30
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1
J14
R60
R70
C87
C86
POLB
AVCC
+12
VCC2
VEE2
+5
-7.5
GND
GND
GND
GND
VIN-
AVEE
S1B
S1W
VIN+
POLW
EPROM
P/N
D/N assy.
PROBE
060002
C60002
FIT RECHARGEABLE BATTERIES ONLY
BLACKWHITE
POL.
NORM
BATT
Figure 2 Eprom Installation
FUSE F2
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9. Refer to Figure 2 to help you find the location of the fuse F2. This 1 amp fuse must be removed and
replaced with the 2 amp fuse provided. Carefully remove the old 1 amp fuse by grasping the green
body with your hand and pulling on each end until it comes out of the socket. Install the new 2 amp
fuse by carefully pushing each end into the socket (a small amount at a time) with needle-nose pliers.
See Figure 3.
10. Next, discharge any static electricity again, then remove the new YSI 2730 printed circuit board from
its anti-static bag. Install the circuit board where the 2710 turntable board was, as shown in Figure 1,
using the two screws and washers removed earlier. Attach the two ribbon cables in their previous
positions.
11. Using a small adjustable wrench (5/16”, if available) loosen and remove the two locknuts securing the
pump mount side plate from the inside of the instrument as shown in figure 1. Remove the side plate.
Save the locknuts.
12. Install the Monitor Pump/Solenoid Valve Assembly from the inside by positioning the two studs
through the Pump/Valve Mounting Plate. Be certain that the solenoid valve is above the pump. Refer
to Figure 4. Reuse the two 5/16" locknuts that were removed above and secure the Pump/Valve
Assembly.
13. Plug the Pump Motor lead (six conductor) into the socket marked J4 on the 2730 PC board. (The
connector is keyed for proper installation.) Plug the Solenoid Valve lead (two conductor) into the
socket marked J3. Route the leads in such a way as to avoid any interference when the back plate is
rotated back into place.
14. Next swing open the front panel of the 2700 and remove the side access plate on the left side of the
2700 near the front of the instrument. This is the area through which the 2700 Sipper Tube will move
to reach the External Sample Chamber. Again use a small adjustable wrench (5/16”, if available) to
loosen and remove the two locknuts securing the side access plate.
Fi
g
ure 3 Fuse Installation
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Figure 4 Pump Installation
15. Slip the External Sample Chamber assembly over the sheet metal edge of the 2700. Use one of the
5/16" locknuts with the flat washer provided to loosely hold the bracket/chamber assembly in position
for now. See Figure 4 for orientation.
16. Connect the tubing as shown in Figure 4. The 2 inches of small tubing coming from the outside
position of the solenoid valve goes to the sample source. The 2 inches of small tubing from the inside
position of the solenoid valve goes to the antiseptic. Extend these with the silicone tubing provided to
a length that suits your setup. The 6 inches of small tubing coming out of the pump head slips over the
stainless steel chamber inlet. The 6 inches of large tubing coming from the pump slips over the barbed
chamber outlet. The remaining 3 foot length of large tubing goes to a suitable waste container (not
included).
17. Close the rear panel door and replace the two screws which hold it. Be careful not to snag any cables
when closing the door.
18. Install the supplied label on the back of the instrument to indicate that it is equipped with the 2730
hardware. This is intended to prevent others from using the instrument with the turntable (doing so
would damage the 2730 circuits).
19. Install the supplied tubing installation diagram on the side of the 2700 case as shown in Figure 5.
20. Repower the instrument. The printer should print a heading showing 2700 configuration (single or
dual channel) and the new software version (2.81).
21. Next align the Sipper Tube with respect to the 2700 Sample Chamber as you would do in 2700 Setup.
Very likely this has already been done on your unit.
External Sample
Chamber
From Antiseptic
(inner)
From Sample
Source
(outer)
To Waste
Monitor Pump
Solenoid Valve
Chamber Outlet
Chamber Inlet
Pump/Valve
Mounting Plate
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Figure 5 Tube Routing Label
22. Activating monitoring software. Refer to Software Flow Chart (Figure 9). Press the MENU key, then
choose 2-Setup, then 3-RunMode, then 4-Monitor. Six selections are shown. Set the Monitor default
parameters to those shown below, confirming each by pressing the ENTER key. You may change
parameters later to fit your application.
•Purge time change 0 to 40 seconds.
•Station # change 1 to 5 (note #4 is for turntable position)
•Interval change 0 to 30 minutes. This is time between samples.
•Precal 0
•Flow 2000
•Antiseptic YES
23. Set up a waste container, a test sample solution (e.g., glucose standard), and a test antiseptic solution
(e.g., reagent water). You may want to add some food dye to the test sample solution to better
visualize what is happening.
24. Press the MENU key to return to the Main Menu. Again press the MENU key, then 1-Service, then
5-Monitor. This menu level allows you to align the Sipper Tube with the External Sampling Chamber
and to prime the External Pump (Purge Pump).
25. Select 3-External Chamber to move the Sipper Tube to Station #5 (external chamber). Now adjust the
Chamber Bracket forward or backward to align the Sipper, and the Thumb Screw to adjust the
chamber side to side. Visually align the Sipper over the conical opening to the External Chamber.
26. Now test the alignment using the "Sipper down" command. Tighten the locknut on the inside of the
2700 and the thumb screw under the Chamber Bracket while the Sipper Tube is in the External
Chamber. Use the up and down commands to retest. Press 0 to exit.
27. Select 1-Pump Sample to prime the inlet line. The test solution should move through the solenoid
valve to the peristaltic pump, then on to the chamber inlet. Eventually segmented flow (air and fluid)
will be observed in the waste line exiting the chamber toward the pump. The pump shuts off after 40
seconds, as programmed. You may shut off the pump at any time by pressing the [1] key.
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28. Select 2-Pump Antiseptic to prime the antiseptic line. The test antiseptic solution should move
through the solenoid valve to the peristaltic pump, then on to the chamber inlet. Segmented flow (air
and fluid) will be observed in the waste line exiting the chamber toward the pump. The pump shuts
off after 35 seconds. You may shut off the pump at any time by exiting the Service menu.
29. The Monitor and Control Accessory is now installed. Now configure the 2700 SELECT measurement
and run mode parameters as required for your application. Refer to Section 2 Setup in your 2700
SELECT USER’S MANUAL.
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All monitor parameters are configured through the 2700 software. See Section 5.2 SETUP to configure the
monitor parameters.
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The factory default settings for the Analog Outputs are +5 VDC Full scale for both channels. The default
configuration for the polarity of the Black, White and Filtrate Pump Control Outputs is normal (not
inverted). See the following sections if you have special Analog/Control Output requirements.
3.2.1 Full Scale Voltage
The Digital-to-Analog Converters on the YSI 2730 circuit board may be individually configured to provide
either +5 volts or +10 volts full scale. The JP1 jumper positions 3 through 6 control this parameter.
Positions 3 and 4 affect the white channel output and positions 5 and 6 affect the black channel output.
Figure 6 identifies the parameter controlled at each jumper position.
Be sure to re-program all appropriate parameters before using
the 2700.
WARNING: Only one jumper per output should be installed!
Installing two jumpers per channel could damage the circuit
components.
JP1 SETTINGS
JUMPER POSITION EFFECT
1 AUX 10 = RAW 17 VOLTS
2 AUX 10 = REG 13.0 VOLTS
3 WHT DAC FULL SCALE = +5V
4 WHT DAC FULL SCALE = +10V
5 BLK DAC FULL SCALE = +5V
6 BLK DAC FULL SCALE = +10V
Figure 6 JP1 Settings
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3.2.2 Full Scale Concentration
The voltage output of the analog interface is scaled relative to the calibration concentration. The user can
configure the analog output for each channel to represent 1, 2, 3, or 4 times the calibration concentration.
These outputs are not configured on the PCB. This configuration option is provided via software in the
setup menu. See Section 5.2.6 ANALOG/CONTROL to set this parameter.
3.2.3 Discrete Output Polarity
Three of the discrete control outputs can be configured to obtain either normal or inverted output. These
three outputs are the Black channel pump control, the White channel pump control and the filtrate pump
control output. Switch SW1 on the 2730 PCB contains four dip switches. Switches 1 through 3 control the
outputs. Switch 4 is not used. Figure 7 depicts the relationship between the switch placement and the
polarity of each output.
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Several methods exist for obtaining the sample for analysis on the YSI 2700. For discrete sampling the
fixed stations (#2 and #3) may be adequate. In many process monitoring and/or control applications the
sample must be transported (pumped) to the analyzer. When configured appropriately the 2700 SELECT
automatically samples a bioreactor, process stream, or other suitable sample source. Since color, turbidity,
optical density and many other physical factors do not affect the YSI enzyme biosensor, filtration and/or
dilution may not be necessary and the 2700/2730 may draw the sample directly. If cell loss is a concern, or
if high cell density is expected, a filtration device (e.g., tangential flow filter) which separates broth and
cells may be installed between the sample source and the 2730 Monitor and Control accessory.
4.1.1 Filtrate Pump
The filtrate pump control output from the YSI 2730 is provided to control a user-supplied external pump
for pumping the sample from its point of origin to the 2700. The timing of this signal is the same as the
purge pump of the YSI 2730 accessory and is setup inside the "Monitor" part of the RunMode menu. This
option is normally not used since the 2730 monitor pump can transport most samples to the external
chamber. If an external filtrate pump is used, the flow rate must be maintained at or above 570µL/minute
in order to guarantee that the sipper does not aspirate air during the sampling process.
SW1 Settings for Output Polarity
Output Switch Closed Open
Black 1 Inverted Normal
Filtrate 2 Inverted Normal
White 3 Inverted Normal
Not Used 4 N/A N/A
Figure 7 Output Polarity
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4.1.2 Examples
Monitor Only Monitor with 2730 Control
Monitor with Computer Control
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4.2.1 Analog Outputs
The YSI 2700 analyzer is capable of performing an analysis approximately every two minutes; it is
therefore incapable of producing a truly continuous signal which represents the instantaneous analyte
concentration. It is because of this "semi-continuous" nature of the instrument that the YSI 2730 interface
provides additional signals to aid in synchronizing the reading of the analog outputs. In addition to the two
analog outputs (one output per chemistry, black and white channels), two logical signals are provided.
These "handshake signals" are nominally +5 volts for a logic 1 and ground (0 volts) for a logic 0. The
"READY" signal is output from the YSI 2730 and is set to a logic 1 when the analog output signals have
been updated. This signal indicates to the host system that the analog voltages are "new" and that they
represent the most recent reading of the analyte concentrations. The host system (the external system to
which the YSI 2730 is connected) then can send a logic 0 to the "ACK" input of the YSI 2730. This
"ACK" (acknowledge) signal response from the host resets the READY line of the YSI 2730 to its low
state before the next sample is ready. Figure 8 shows the typical signal pattern that would occur during
two sample update cycles. The READY signal will reset itself immediately prior to updating the analog
outputs if not reset externally via the ACK\ signal input.
4.2.2 Pump Control Outputs
Three discrete outputs are provided on the YSI 2730 which are intended to control external pumps. The
means of control is on or off only, no intermediate states are supported. The three outputs are labeled
Black Channel Pump, White Channel Pump and Filtrate Pump. All of these outputs are electrically
identical and are designed as control signals only, i.e. they are incapable of driving any pumps directly.
These signals must be buffered externally in a manner appropriate to the nature of the pumps being used.
These output signals transition between +5 volts and 0 volts nominally. The logic of each, i.e. whether 5
volts turns on or off the external device, is determined by the position of switch SW1 on the 2730 PC board
(See Section 3.2.3). Assuming no inversion enabled by the user, the output turns on the pump with a +5
voltage output, exclusive of any logic inversion imposed by the user supplied buffering circuitry.
Analog Output
Ready
Ack\
Figure 8 Handshake Signals
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4.2.3 Auxiliary Connector / Signal List
On the back of the YSI 2700 instrument is the 15 pin "D" type connector labeled "AUXILIARY" where the
2730 signals emanate. The following table relates the signals with the connector pin positions and cable
wire colors.
2730 Signal AUXILIARY pin# Wire color
Ground 1 Black/White
Ground 2 Orange/Black
Ground 3 Blue/Black
Ground 4 Red/White
Ground 5 Black
+5 Volts 6 White
White Pump Control 7 Green/White
SysErr 8 Blue/White
White Analog Output 9 Green/Black
Black Analog Output 10 Green
Black Pump Control 11 Red/Black
Filtrate Pump Control 12 Red
Power Out 13 Blue
Ack\ 14 White/Black
Ready 15 Orange
Chassis Ground None Shield
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IMPORTANT: The following instructions were written assuming a familiarity with the YSI 2700 SELECT
Biochemistry Analyzer. If you ordered the 2700 and 2730 together and are setting up for the first time,
you should refer to the 2700 SELECT USER’S MANUAL for instructions. Once you have successfully
set up and operated the 2700 in the discrete sampling mode, you may proceed with the 2730 Monitor and
Control Accessory installation and setup.
If you are familiar with YSI 2700 SELECT operation, you may proceed with the setup and operation
instructions that follow.
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14
814# 6RIWZDUH
Features specific to the YSI 2730 have been added to the Setup and Service sections of the 2700 menu
structure. Figure 9 shows the new structure with the 2730 features in bold lettering. The following section
briefly describes the 2730 menu options.
Figure 9 Software Flow Chart
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15
815# 6HWXS
5.2.1 Monitor
As described in Section 2, Installation, there are six menu selections under Monitor Setup that you must
consider. Briefly, they are:
External Pump Purge Time........Time in seconds required to deliver fresh sample from your source to the
External Sampling Chamber
Station #.....................................Station number where the Sipper Tube will go for a monitor sample,
usually station #5 the external sampling chamber
Interval.......................................Time in minutes between monitor samples
PreCal ........................................Time in minutes before the monitor sample when an autocalibration will
be initiated
Flow...........................................The flow rate of the monitor pump in uL/min
Antiseptic...................................Antiseptic cycle on or off
Flow Rate -vs- Purge Time
The flow rate and purge time settings must be such that a sufficient volume of sample is pumped during
each cycle to completely purge the external chamber and system tubing. This is especially important when
an antiseptic is used since the antiseptic may damage the enzyme membrane if aspirated by the sipper
needle. Figure 10 shows the flow rates and minimum time needed to purge the tubing lines from the
solenoid valve to the external sample chamber. These values are based upon a 6-fold volume turnover.
Additional time may be necessary to purge the line from the sample source to the solenoid valve.
Figure 10 Minimum Purge Time
5.2.2 Antiseptic
The antiseptic solution may be defined by the user. Typical solutions might include 1% sodium hydroxide
or 0.25% hypochlorite in reagent water.
Flow Rate
uL/min Purge Time Seconds Flow Rate uL/min Purge Time Seconds
100 900 1400 65
200 450 1500 60
300 300 1600 57
400 225 1700 53
500 180 1800 50
600 150 1900 48
700 129 2000 45
800 113 2100 43
900 101 2200 41
1000 90 2300 39
1100 82 2400 38
1200 75 2500 36
1300 69
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