YSI 1500 Sport User manual

www.YSI.com
User's Manual
YSI 1500 Sport
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-Lactate Analyzer
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Lactate Analyzer

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Table of Contents
1. Safety.................................................................................................... 1-1
1.1 Explanation of Symbols............................................................................................... 1-1
2. Introduction .......................................................................................... 2-1
2.1 Features........................................................................................................................2-1
2.2 Specifications............................................................................................................... 2-2
3. Instrument Setup .................................................................................. 3-1
3.1 Unpacking.................................................................................................................... 3-1
3.2 Component Identification ............................................................................................ 3-2
3.3 Reagent Preparation..................................................................................................... 3-4
Automatic Mode .............................................................................................................. 3-4
Manual Mode.................................................................................................................. 3-5
3.4 Membrane Installation ................................................................................................. 3-6
3.5 Power ...........................................................................................................................3-6
3.6 System Setup ............................................................................................................... 3-7
Date.................................................................................................................................. 3-9
Time............................................................................................................................... 3-10
RS232 Communication.................................................................................................. 3-11
Setting the LCD Contrast............................................................................................... 3-12
Displaying Numerical Values in RADIX ...................................................................... 3-13
Setting the Stirring Speed .............................................................................................. 3-13
Selecting the Displayed Units........................................................................................ 3-14
Selecting the Operational Mode .................................................................................... 3-15
Resetting System Default Parameters............................................................................ 3-16
3.7 Preparing the Fluid System........................................................................................ 3-18
3.8 Printer Option ............................................................................................................ 3-19
4. Sample Collection, Preparation and Measurement............................ 4-1
4.1 Sample Preparation...................................................................................................... 4-1
YSI Model 1501, 25 microliter Syringepet ..................................................................... 4-2
YSI Model 1502 Capillary Tube Injector........................................................................ 4-3
5. Principles of Operation ........................................................................ 5-1
5.1 Sensor Technology ...................................................................................................... 5-1
5.2 Fluid System ................................................................................................................ 5-3
5.3 Measurement Methodology ......................................................................................... 5-4
5.4 Baseline Stability......................................................................................................... 5-6

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5.5 Calibration.................................................................................................................... 5-7
5.6 Linearity.......................................................................................................................5-8
5.7 Temperature Compensation ......................................................................................... 5-9
5.8 Software Structure ....................................................................................................... 5-9
Microprocessor Self Test ................................................................................................. 5-9
Electrode Checks ............................................................................................................. 5-9
Temperature Error Checks ............................................................................................. 5-10
Battery Level Checks..................................................................................................... 5-10
1500 SPORT Menu Flow Chart..................................................................................... 5-11
6. Instrument Operation .......................................................................... 6-1
6.1 Calibration Cycle ......................................................................................................... 6-1
Calibration-Automatic Mode ........................................................................................... 6-1
Calibration-Manual Mode................................................................................................ 6-5
6.2 Sample Cycle ............................................................................................................... 6-8
6.3 Recalling Stored Results............................................................................................ 6-10
Recalling All Sample Results ........................................................................................ 6-10
Recalling Today's Sample Results................................................................................. 6-11
Recalling a Result From Any Previous Date ................................................................. 6-12
Recalling a Result By ID Number ................................................................................. 6-14
6.4 Printing Results.......................................................................................................... 6-15
Printing All Sample Results........................................................................................... 6-15
Printing Today's Results ................................................................................................ 6-16
Printing Previous Days' Results..................................................................................... 6-17
Printing Results By ID Number..................................................................................... 6-17
6.5 Transmitting Results Over the RS232 Communication Link.................................... 6-18
6.6 Deleting Stored Results.............................................................................................. 6-19
Editing Results............................................................................................................... 6-19
Deleting All Results....................................................................................................... 6-20
6.7 Daily Operational Checks .......................................................................................... 6-21
7. Instrument Diagnostics ........................................................................ 7-1
7.1 Checking Probe Current............................................................................................... 7-1
7.2 Checking Sample Chamber Temperature .................................................................... 7-2
7.3 Checking the Sample Chamber Injection Switch Status.............................................. 7-3
7.4 Turning the Stir Motor On and Off.............................................................................. 7-3
7.5 Turning Pump Motors On and Off............................................................................... 7-4
7.6 Sending a Test Print String Over the Infrared Data Link ............................................ 7-5
7.7 Sending a Test Data String Over the RS232 Data Link............................................... 7-5
7.8 Testing the Keypad ...................................................................................................... 7-6
7.9 Testing the Display ...................................................................................................... 7-7

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7.10 Checking the Software Version Number ................................................................. 7-8
7.11 Checking the Battery Voltage.................................................................................. 7-8
8. Maintenance......................................................................................... 8-1
8.1 Daily Maintenance....................................................................................................... 8-1
Empty the Waste Bottles ................................................................................................. 8-1
Check Buffer and Reference Bottle Levels ..................................................................... 8-1
Check for Air Bubbles in Chamber ................................................................................. 8-1
Check for Leaks............................................................................................................... 8-2
Clean up spills.................................................................................................................. 8-2
8.2 Preventative Maintenance............................................................................................ 8-2
8.3 Cleaning the Sample Chamber .................................................................................... 8-2
8.4 Membrane Replacement .............................................................................................. 8-3
8.5 Probe Cleaning............................................................................................................. 8-3
8.6 Tubing Replacement.................................................................................................... 8-4
8.7 Injection Port Seal Replacement.................................................................................. 8-6
8.8 Recharging the Battery ................................................................................................ 8-6
8.9 Storing the SPORT ...................................................................................................... 8-6
9. Troubleshooting ................................................................................... 9-1
9.1 Fault Messages............................................................................................................. 9-2
Data System Faults .......................................................................................................... 9-2
Battery Faults................................................................................................................... 9-4
Enzyme Sensor Faults...................................................................................................... 9-4
Temperature Faults .......................................................................................................... 9-8
Fluid System Faults ......................................................................................................... 9-9
9.2 Possible Failure Conditions ....................................................................................... 9-10
10. Appendix A–Typical Performance Data .............................................10-1
Capillary Tube Injector, YSI Model 1502..................................................................... 10-1
Blunt Needle Syringepet, YSI Model 1501................................................................... 10-3
11. Appendix B–Sample Collection and Handling ................................... 11-1
Blood Plasma................................................................................................................. 11-1
Blood Serum .................................................................................................................. 11-2
Whole Blood.................................................................................................................. 11-3
12. Appendix C–Cell Lysing ...................................................................... 12-1
Using the YSI 1515 Cell Lysing Agent......................................................................... 12-2
Buffer Solution .............................................................................................................. 12-2
Reference Solution......................................................................................................... 12-2
13. Appendix D Sample Preservation ...................................................... 13-1
14. Appendix E–Effects Of Selected Substances ....................................... 14-1