Zeiss Axiovert 40 User manual

Operating Manual
Axiovert 40
Inverted Microscope

Unless expressly authorized, forwarding and duplication of this document and the softĆ
ware package, as well as utilization and communication of its contents are not permitted.
Violations will entail an obligation to pay compensation.
The manual is not covered by an update service.
umber of
this manual: B 46-0031 e
Date of issue: 11/25/2003

Issued by: Carl Zeiss
Light Microscopy
P.O.B. 4041
D-37030 Göttingen
Phone: [phone]
Telefax: [phone]
Internet: www.zeiss.de/micro
EĆmail: [email protected]

Axiovert 40
B 46-0031 e 11/03 I
CONTENTS
Page
Title page
Copyright
Contents I. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
List o illustrations IV. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Note VI. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
General view VII. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Notes on instrument sa ety IX. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1 DESCRIPTION
1.1 Name and intended use 1Ć1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.2 Instrument description 1Ć1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.2.1 Mechanical design 1Ć3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.2.2 Optical design 1Ć4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.3 Technical data 1Ć5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.4 System overview 1Ć8. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Axiovert 40
11/03 B 46-0031 e
II
Page
2 OPERATION
2.1 Installation o the microscope 2Ć1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.1.1 Unpacking 2Ć1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.1.2 Installation 2Ć2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.2 Start-up 2Ć8. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.3 Use o LD objectives 2Ć9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.4 Illumination and contrast techniques 2Ć11. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.4.1 Use o condenser 2Ć11. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.4.2 Bright ield illumination 2Ć12. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.4.3 Phase contrast 2Ć13. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.4.4 VAREL 2Ć15. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.4.5 PlasDIC 2Ć17. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.4.6 Incident-light luorescence 2Ć19. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.5 Working with attachable object traverser M and mounting rames 2Ć20. . . . . . . . . . . .
2.6 Counting, measuring and raming reticles 2Ć21. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.7 Working with micromanipulators 2Ć22. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.8 Replacing the specimen stage 2Ć23. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.8.1 Specimen stage glass 2Ć24. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.8.2 Heatable stage and temperable microscope stage 2Ć24. . . . . . . . . . . . . . . . . . . . . . . . . .
2.9 Documentation 2Ć25. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Axiovert 40
B 46-0031 e 11/03 III
Page
3 MAINTENANCE AND TROUBLESHOOTING
3.1 Maintenance 3Ć1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.2 Troubleshooting and service 3Ć2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
ANNEX
- Index AĆ3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
- List o abbreviations AĆ7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Axiovert 40
11/03 B 46-0031 e
IV
LIST OF ILLUSTRATIONS
Page
Fig. 1Ć1 Instrument models 1Ć3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 1Ć2 Optical diagram o Axiovert 40 1Ć4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 1Ć3ā/ā1 Axiovert 40 system overview 1Ć8. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 1Ć3ā/ā2 Axiovert 40 system overview 1Ć9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 1Ć3ā/ā3 Axiovert 40 system overview 1Ć10. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć1 Axiovert 40 packing units 2Ć1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć2 Unpacking and installation 2Ć2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć3 Screwing in objectives 2Ć2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć4 Inserting diaphragm sliders 2Ć3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć5 Mounting the HBO 50 illuminator 2Ć3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć6 Attaching the attachable object traverser M 2Ć4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć7 Connection to the power outlet 2Ć5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć8 Inserting the eyepieces 2Ć6. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć9 Positions o the binocular tube 2Ć7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć10 Inserting an eyepiece reticle 2Ć7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć11 Switching on/setting the instrument 2Ć8. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć12 Working with LD objectives 2Ć9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć13 Condenser 0.55 2Ć12. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć14 Various modes o bright ield illumination 2Ć12. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć15 Observation in phase contrast 2Ć13. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć16 Illustration o phase-contrast adjustment 2Ć13. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć17 Inserting the annular diaphragms 2Ć14. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć18 Observation with VAREL 2Ć15. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć19 VAREL with microtiter plates 2Ć15. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć20 Pupil images with VAREL 2Ć16. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć21 Slider Var, H, Var 2Ć16. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć22 Observation with PlasDIC 2Ć17. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć23 Slider Ph, H, PlasDIC 2Ć18. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć24 Observation in incident-light luorescence 2Ć19. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Axiovert 40
B 46-0031 e 11/03 V
Page
Fig. 2Ć25 Working with attachable object traverser M 2Ć20. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć26 Selected eyepiece reticles and stage micrometers 2Ć21. . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć27 Mounting acilities or micromanipulators 2Ć22. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć28 Replacing the specimen stage 2Ć23. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć29 Mounting the specimen stage glass 2Ć24. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć30 Camera system 2Ć25. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 2Ć31 Adjusting the digital camera adapter 2Ć26. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 3Ć1 Checking the line voltage 3Ć2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 3Ć2 Replacing the halogen bulb 3Ć3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 3Ć3 HBO 50 illuminator with line spectrum 3Ć4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 3Ć4 Replacing the bulb o the HBO 50 3Ć5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 3Ć5 Adjustment o HBO 50 3Ć7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 3Ć6 Lamp image 3Ć7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 3Ć7 Replacing the re lector modules FL P&C 3Ć8. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 3Ć8 Changing the luorescence ilter set 3Ć9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 3Ć9 Inserting ilters and dichroic beam splitter 3Ć9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 3Ć10 Changing the dichroic beam splitter 3Ć10. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 3Ć11 Mounting the dichroic beam splitter 3Ć10. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fig. 3Ć12 Marking o dichroic beam splitter 3Ć11. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Axiovert 40
11/03 B 46-0031 e
VI
NOTE
•The igures integrated in the text are numbered and captioned.
"Figure 2-8", or example, re ers to igure No. 8. Items re erred to in the text are marked by a re erence
line and an item number. For example, eyepiece tube (2-8/4)" means that the eyepiece tube in igure
8 in section 2 is marked with the item number 4.
•Abbreviations are explained in the Annex.
•This operating manual re ers to the Axiovert 40 con igurations (see section 1.4) including accessories.
When working with other instrument models, the instructions apply analogously.

Axiovert 40
B 46-0031 e 11/03 VII
GENERAL VIEW
Axiove t 40 CFL

Axiovert 40
11/03 B 46-0031 e
VIII

Axiovert 40
B 46-0031 e 11/03 IX
NOTES ON INSTRUMENT SAFETY
The Axiovert 40 microscopes have been designed, produced and tested in compliance with DIN 61010-1
(IEC 1010-1) and DIN EN 61010-2-101 sa ety requirements or electrical measuring, control and laboratory
instruments, and meet the requirements o Appendix I o directive 98/79/EC.
Con ormity to the given standards is documented by the marking.
This operating manual includes in ormation and warnings that must be observed by the user.
NOTE
This symbol is a warning, which you must observe under all circumstances.
CAUTION
This symbol is a warning, which indicates a hazard to the instrument or instrument system.
CAUTION
This symbol is a warning, which indicates a hazard to the user of the instrument.
CAUTION
Hot surface!
CAUTION
Emission of UV radiation!
CAUTION
Disconnect the instrument from the line before o ening it!

Axiovert 40
11/03 B 46-0031 e
X
The Axiovert 40 microscopes, including original accessories, may only be used or the microscope techniques
and applications described in this manual.
Particular attention must be paid to the ollowing warnings:
The manu acturer cannot assume any liability or any other applications, including that o individual
modules or single parts. This also applies to all service or repair work that is not carried out by authoĆ
rized service personnel. All warranty claims shall be or eited.
Axiovert 40 Inverted Microscopes are classi ied as Protection Class I instruments.
The power plug must be inserted in an outlet eaturing a grounding (earth) contact. The grounding
e ect must not be made ine ective by an extension cable that does not have a protective ground
wire.
I it is determined that protection measures are no longer e ective, the instrument must be
switched o and sa eguarded against inadvertent operation. Please contact a Zeiss service agency
or the Carl Zeiss Microscopy Service to repair the instrument.
The Axiovert 40 Inverted Microscopes are equipped with a new, wide input range power supply
allowing line voltages to be used in the range between 100 and 240 V AC ±10 %, 50 / 60 Hz,
without the voltage setting on the instrument having to be changed.
Be ore switching on the HBO 50 power supply, check whether it is suitable or the available line
voltage. Always disconnect the instrument rom the power outlet be ore opening the instrument
and be ore changing the uses.
Take care to ensure you only use uses o the rated power required. Use o makeshi t uses and
short-circuiting o the use holders are not permitted.
The Axiovert 40 Inverted Microscopes are not equipped with any special devices or protection rom
substances that are corrosive, potentially in ectious, toxic, radioactive, or other substances that
could be hazardous to health. Make sure to observe all legal regulations, particularly the relevant
national accident prevention regulations when handling such substances.
Be sure to read the sa ety notes provided with Immersol 518 N immersion oil.
Immersol 518 N immersion oil irritates the skin. Avoid any contact with skin, eyes and clothing.
A ter skin contact, wash the oil o with plenty o water and soap.
A ter eye contact, immediately rinse the eye with plenty o water or at least ive minutes.
I the irritation persists, consult a specialist.
Proper disposal.
Take care to ensure that immersion oil does not enter sur ace water or the sewage system.

Axiovert 40
B 46-0031 e 11/03 XI
Avoid touching the hot lamp housing. Always pull the power plug be ore changing the lamps and
allow the instrument to cool down or some 15 minutes.
When luorescence ilters are used, the heat protection ilter or heat emitted by the microscope
illuminator must not be removed, since luorescence ilters are sensitive to heat, and their per orĆ
mance could be impaired.
Dust and dirt may impair the instrument's per ormance. There ore, the instrument must be protecĆ
ted rom these in luences as ar as possible and covered with the dust cover when not in use. Always
check whether the instrument is switched o be ore you cover it.
Placing objects against or covering ventilation slats may lead to heat build-up that will damage the
instrument and, in extreme cases, cause a ire. Always keep the ventilation slats clear and ensure
that no objects enter the instrument through the ventilation slats.
The instruments may only be operated by trained personnel who must be aware o the possible
dangers involved with microscopy and the particular application concerned. The Axiovert 40 InĆ
verted Microscopes are high-precision instruments that can be impaired in their per ormance or
destroyed when handled improperly.
Gas-discharge lamps, e.g. XBO 75, HBO 50 or HBO 103, emit ultraviolet radiation, which can cause
burns to the eyes and skin. There ore, never look directly into the light o these lamps and avoid
direct, unprotected incidence o their light on your skin. When using the microscope, always use the
protective devices belonging to the instrument (e.g. special attenuation ilters). When they are hot,
gas-discharge lamps are under high internal pressure. There ore, change them only when they have
cooled down, and make sure to wear protective gloves and goggles ( or detailed in ormation please
see Operating Manual B 40-065 e).
De ective microscopes must not be disposed o with household waste. Dispose o the microscope in
compliance with the relevant legal requirements.

Axiovert 40
11/03 B 46-0031 e
XII

Axiovert 40
B 46-0031 e 11/03 1Ć1
1 Description
1.1 Name and intended use
Manufacturer's designation: Axiovert 40 Inverted Trans itted-Light Microscope
In the product fa ily of inverted trans itted-light icroscopes, the Axiovert 40 icroscopes rank as follows:
Laboratory microscopes Research microscopes
- Axiovert 40 C - Axiovert 200
- Axiovert 40 CFL - Axiovert 200 M
The Axiovert 40 icroscopes are universally applicable odular light icroscopes of inverted design (inverted
icroscopes). They are used in biology and edicine for the exa ination of plant and ani al cells and/or
tissue speci ens, but also of speci ens of the hu an body. Observation and cultivation vessels include, for
instance, culture bottles, Petri dishes and icrotiter plates.
The following icroscopy techniques are possible:
Trans itted light - Brightfield
- Phase contrast
- VAREL
• Oblique brightfield illu ination
• Unilateral darkfield illu ination
- PlasDIC
Incident light - Fluorescence
1.2 Instrument description
The Axiovert 40 icroscopes are available as high-perfor ance desktop units with two different icroscope
stand versions:
-Axiovert 40 C Inverted trans itted-light icroscope with
HAL 12 V 35 W illu inator and docu entation
-Axiovert 40 C L Inverted trans itted-light/incident-light fluorescence icroscope
with HAL 12 V 35 W and HBO 50 illu inators and docu entation

Axiovert 40
11/03 B 46-0031 e
1Ć2
Major instru ent features include:
•Modular design for opti u adaptation to applications
•Microscope stand of co pact design; ergono ic operation provided by a stage height of only 185
on Axiovert 40 C and low-positioned controls
•Integrated 12 V 35 W power supply
•Continuously adjustable illu ination intensity
•Upright, non-reversed i ages
•Fixed Köhler illu ination, changeable condenser, nu erical apertures of 0.55, 0.4 or 0.2 and working
distances of w 31 , w 53 or w 90 ; brightfield illu ination for large object fields for vessel
heights of up to 190
•User-friendly phase contrast by eans of slider with two annular diaphrag s
•Brightness co pensation by attenuation filters providing dazzle-free switching between phase contrast
and brightfield illu ination
•VAREL (variable relief) with continuous transition fro unilateral darkfield to oblique brightfield, contrastĆ
ing of the wells of icrotiter plates up to the edge without special preparations
•PlasDIC for relief-like i aging especially of thick objects with variable contrasting, contrasting of the wells
of icrotiter plates up to the edge
•Ph and PlasDIC: Si ple changeover to phase contrast or PlasDIC
•Incident-light fluorescence contrast usable alternatively to or si ultaneously with brightfield, phase conĆ
trast and VAREL
•Possibility of adapting incident-light fluorescence to the respective application by replacing the fluoresĆ
cence filter co bination
•Variable stage use by ounting the attachable object traverser M
•Use of different ounting fra es including arker strips for various culture vessels
•Speci en stage can be replaced by speci en stage glass for opti u visibility of the nosepiece, a heatable
stage or a te perable icroscope stage for de anding experi ents
•Standardized ounts for objectives, eyepieces and la ps
•Photo/video port for single-reflex ca eras, co pact digital ca eras, digital ca eras, e.g. AxioCa , and
video ca eras

Axiovert 40
B 46-0031 e 11/03 1Ć3
1.2.1 Mechanical design
Axiovert 40 C stand
•Integrated wide input range power supply powerĆ
ing a 12 V 35 W halogen la p and accepting line
voltages of 100 to 240 V AC without the need for
changing the voltage setting
•NA 0.55, 0.4 and 0.2 condensers; replaceable and
ovable
•Coaxial coarse and fine drive with focusing acting
on the nosepiece
•Binocular tube adjustable to two heights with one
fixed and one focusing eyepiece
•Quintuple nosepiece
•Speci en stage prepared for fitting the attachĆ
able object traverser M with the possibility of usĆ
ing different ounting fra es
•Optional diaphrag sliders for VAREL and phase
contrast
•Photo/video port
Axiovert 40 C L stand
•Sa e as Axiovert 40 C
•Optional diaphrag sliders for VAREL, phase conĆ
trast and PlasDIC
•Upgradeable for fluorescence by HBO 50, FL inciĆ
dent-light fluorescence equip ent, 3-position reĆ
flector slider P&C, reflector odules FL P&C with
fluorescence filter sets
ig. 1Ć1 Instrument models

Axiovert 40
11/03 B 46-0031 e
1Ć4
1.2.2 Optical design
(shown using the exa ple of the Axiovert 40 CFL)
The tried and tested ICS optics (Infinity Color-corrected Syste ) guarantees high optical perfor ance for all
ethods (up to field-of-view nu ber 20, tube factor 1x). Different co binations of objectives and eyepieces
provide opti u custo ization to the intended application.
Depending on the configuration, the Axiovert 40 icroscopes are equipped with a 12 V 35 W halogen la p
and a HBO 50 illu inator.
An universal port is available for docu entation. Type-specific adapter odules are available for photo iĆ
crography and video icroscopy.
The correct color te perature for color photography is obtained auto atically at full la p voltage.
A light path selector directs 100 % of light either to the viewing or the photo/video port.
ig. 1Ć2 Optical diagram of Axiovert 40