Zeiss Stemi DV4 User manual

Stereomicroscopes
Stemi DV4
Stemi DV4 SPOT
Stemi DR 1040
Stemi DR 1663
Operating Instructions

COPYRIGHT
Carl Zeiss Stemi DV4 / DR
0-2 B 40-003 e 08/00
Knowledge of this manual is required for the operation of the instrument. Would you please therefore
make yourself familiar with the contents of this manual and pay special attention to hints concerning the
safe operation of the instrument.
The specifications are subject to change; the operating manual is not covered by an update service.
© Unless expressly authorized, forwarding and duplication of this document, and the utilization and
communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
Issued by: Carl Zeiss
Light Microscopy
P. O. B. 4041
37030 Göttingen
GERMANY
Telephone: ++49 551 5060 660
Telefax: ++49 551 5060 464
Internet: www.zeiss.de/micro
E-Mail: [e-mail]
Number of this manual: B 40-003 e
Date of issue: 31.08.2000

CONTENTS
Stemi DV4 / DR Carl Zeiss
B 40-003 e 08/00 0-3
CONTENTS
Page
Copyright ..................................................................................................................................0-2
Contents ..................................................................................................................................0-3
Illustrations ..................................................................................................................................0-5
1 INTRODUCTION ........................................................................................................1-1
1.1 General notes .............................................................................................................1-1
1.2 Notes on instrument safety..........................................................................................1-2
1.3 Notes on warranty ......................................................................................................1-2
1.4 General use of stereomicroscopes................................................................................1-3
2 DESCRIPTION: ...........................................................................................................2-1
2.1 Stemi DV4 / DR product family....................................................................................2-1
2.2 Compact version with stand C.....................................................................................2-2
2.2.1 System diagram ..........................................................................................................2-2
2.2.2 Interfaces of the compact version................................................................................2-3
2.2.3 Mobile equipment C for Stemi DV4, Stemi DR.............................................................2-3
2.2.4 Technical Data ............................................................................................................2-4
2.3 Modular Zeiss Stemi system.........................................................................................2-6
2.3.1 System diagram (extract).............................................................................................2-6
2.3.2 Interfaces in the modular system.................................................................................2-7
3 OPERATION...............................................................................................................3-1
3.1 Basic instrument as compact version with stand C .......................................................3-1
3.1.1 Start-up......................................................................................................................3-1
3.1.2 Operation and function controls..................................................................................3-2
3.1.3 Illumination.................................................................................................................3-3
3.1.3.1 Selection of the illumination mode ..............................................................................3-3
3.1.3.2 Varying the illumination intensity.................................................................................3-4
3.1.3.3 Specimen protection in transmitted-light applications...................................................3-4
3.1.4 Basic setting of the stereomicroscope..........................................................................3-5
3.1.5 Lamp change..............................................................................................................3-7
3.1.5.1 Change of reflected-light lamp....................................................................................3-7
3.1.5.2 Change of transmitted-light lamp................................................................................3-8
3.1.6 Setting the smoothness of the focusing drive...............................................................3-9

CONTENTS
Carl Zeiss Stemi DV4 / DR
0-4 B 40-003 e 08/00
3.2 Core accessories.......................................................................................................3-10
3.2.1 Attachment for transmitted-light darkfield ................................................................3-10
3.2.2 Direct measurement using the eyepiece measuring device 8x/32x/18
on the Stemi DV4.....................................................................................................3-11
3.2.3 Documentation of microscopic images...................................................................... 3-12
3.2.3.1 SLR camera...............................................................................................................3-12
3.2.3.2 SONY camera "Digital Handycam DCR-PC100".........................................................3-13
3.2.3.3 SONY camera "Digital Still Camera Cybershot DSC-F505"......................................... 3-14
3.2.3.4 SONY camera "Digital Still Camera Cybershot DSC-S70"........................................... 3-15
3.2.3.5 Video camera with C-Mount connector.....................................................................3-17
3.2.3.6 Microhead camera.................................................................................................... 3-17
3.2.3.7 Eyepiece insertion camera......................................................................................... 3-18
4 MAINTENANCE AND SERVICE.................................................................................. 4-1
4.1 Maintenance ..............................................................................................................4-1
4.2 Consumables..............................................................................................................4-1
4.3 Service........................................................................................................................4-2
ANNEX
Certification according to DIN ISO 9001 / EN 46001
EC Declaration of Conformity

ILLUSTRATIONS
Stemi DV4 / DR Carl Zeiss
B 40-003 e 08/00 0-5
ILLUSTRATIONS
Page
Fig. 2-1 Stemi DV4 / DR microscope body.................................................................................2-1
Fig. 2-2 System diagram of compact version with stand C........................................................2-2
Fig. 2-3 Interfaces of Stemi DV4 / DR with stand C (schematic).................................................2-3
Fig. 2-4 Stemi DV4 / DR system diagram within the Zeiss Stemi modular system........................2-6
Fig. 2-5 Interfaces of the Stemi DV4 / DR with column-type stand (schematic)..........................2-7
Fig. 2-6 Front lenses of Stemi DV4 / DR....................................................................................2-7
Fig. 3-1 Installation and mains connection of the Stemi DV4 / DR..............................................3-1
Fig. 3-2 Operation and function controls on the Stemi DV4 / DR...............................................3-2
Fig. 3-3 Illumination control.....................................................................................................3-3
Fig. 3-4 Basic settings (Stemi DV4) ...........................................................................................3-5
Fig. 3-5 Changing the lamp (reflected light) .............................................................................3-7
Fig. 3-6 Changing the lamp (reflected light) .............................................................................3-7
Fig. 3-7 Removing the glass plate.............................................................................................3-8
Fig. 3-8 Changing the lamp (transmitted light).........................................................................3-8
Fig. 3-9 Inserting the glass plate...............................................................................................3-9
Fig. 3-10 Setting the smoothness of the focusing drive...............................................................3-9
Fig. 3-11 Removing the glass plate...........................................................................................3-10
Fig. 3-12 Insertion of transmitted-light darkfield attachment.....................................................3-10
Fig. 3-13 Insertion of eyepiece measurement device.................................................................3-11
Fig. 3-14 Eyepiece micrometer 8x/32x/18.................................................................................3-11
Fig. 3-15 Attachment of SLR camera........................................................................................3-12
Fig. 3-16 Attachment of SONY camera "Digital Handycam DCR-PC100"..................................3-13
Fig. 3-17 Attachment of SONY camera "Digital Still Camera Cybershot DSC-F505" ..................3-14
Fig. 3-18 Attachment of SONY camera "Digital Still Camera Cybershot DSC-S70"....................3-15
Fig. 3-19 Attachment of video camera with C-Mount connector..............................................3-17
Fig. 3-20 Attachment of microhead camera .............................................................................3-17
Fig. 3-21 Attachment of insertion camera................................................................................3-18

ILLUSTRATIONS
Carl Zeiss Stemi DV4 / DR
0-6 B 40-003 e 08/00

INTRODUCTION
Stemi DV4 / DR General notes Carl Zeiss
B 40-003 e 08/00 1-1
1INTRODUCTION
1.1 General notes
NOTE: In this manual, Stemi DV4 / DR always stands for Stemi DV4, Stemi DV4 SPOT,
Stemi DR 1040 and Stemi DR 1663.
The stereomicroscopes Stemi DV4 / DR were designed, produced and tested in compliance with DIN
61010-1 (IEC 1010-1), Safety requirements for electrical measuring, control and laboratory instruments,
and meet the requirements of appendix I of directive 73/23/EC.
The instruments meet the requirements of the EC directive 89/336/EC and the EMC legislation of
November 9th 1992. This operation manual includes information and warnings which must be observed
by the user.
The following warning and information symbols are used in this manual:
☞NOTE
This symbol is a warning which you must observe under all circumstances.
CAUTION
This symbol is a warning which indicates a hazard to the instrument or instrument system.
CAUTION
This symbol is a warning which indicates a hazard to the user of the instrument.
CAUTION
Disconnect the instrument from the line before opening it!

INTRODUCTION
Carl Zeiss Notes on instrument safety and warranty Stemi DV4 / DR
1-2 B 40-003 e 08/00
1.2 Notes on instrument safety
The Stereomicroscopes Stemi DV4 / DR, including original accessories, may only be used for the
applications described in this manual. The manufacturer cannot assume any liability for any
other applications, possibly also involving individual modules or single parts. This also applies to
all service or repair work which is not carried out by authorized service personnel. Furthermore,
this forfeits all the claims against warranty
Always disconnect the power unit from the line before exchanging lamps!
Turning the two zoom/focusing knobs in opposite directions by applying force will result in the
stereomicroscope being damaged.
Dust and dirt can impair the performance of the instrument. Therefore, the instrument must be
protected against these influences as far as possible, and covered with the dust cover if it is not
used for longer periods of time.
1.3 Notes on warranty
The manufacturer guarantees that the instrument has no material and production defects when
delivered. You must inform us of any defects immediately and we must do anything to minimize the
damage. If the manufacturer is informed of such a defect, he is obliged to remove it; it is his decision
whether he does this by repairing the instrument or by delivering an instrument free of any defect. No
guarantee is provided for defects caused by natural wear (this concerns wearing parts in particular) and
improper use.
The instrument manufacturer is not liable for damage caused by faulty operation, negligence or any
other meddling with the instrument, particularly the removal or replacement of instrument components,
or the use of accessories from other manufacturers. This forfeits all the claims against warranty.
With the exception of the work specified in this manual, no maintenance or repair of the microscopes
may be performed. Repairs may only be performed by Carl Zeiss service staff or specially authorized
personnel. Should any defect occur with the instrument, please get in touch with the Carl Zeiss
microscopy service in Germany or your local Zeiss agency.

INTRODUCTION
Stemi DV4 / DR General use of stereomicroscopes Carl Zeiss
B 40-003 e 08/00 1-3
1.4 General use of stereomicroscopes
The stereomicroscopes Stemi DV4 / DR are Greenough-type microscopes mainly designed for versatile
hobby and training applications and for routine work in biology, medicine and industry.
The name DV4 stands for Double Lens Vario with the zoom factor 4. The zoom range of 4:1 corresponds
to the overall magnification range of 8x to 32x (basic version).
The zoom magnification changer guarantees high-contrast, sharp images over the entire zoom range.
After the basic setting of the stereomicroscope, the image remains exactly in focus.
The name DR stands for Double Lens Revolver and means that these stereomicroscopes use two fixed
magnifications which can be switched discretely. Stemi DR 1040 with overall magnifications 10x and
40x, Stemi DR 1663 with 16x and 63x (basic configuration).
Their compact design makes the stereomicroscopes Stemi DV4 / DR ready for immediate use and very
easy to operate. Depending on requirements, these microscopes can be converted and upgraded in
accordance with the modular system of Zeiss stereomicroscopes.
Interchangeable optics components permit the magnification range to be varied as required.
Modern illumination equipment for reflected and transmitted light is integrated in stand C. As a standard
feature, the press of a key is sufficient for the selection of:
−brightfield illumination in reflected light
−brightfield illumination in transmitted light
−brightfield illumination in mixed light
And optional:
−darkfield illumination in transmitted light
The illumination intensity can be set continuously and electronically at the press of a button for each
illumination type and can therefore be matched to most different objects.
Suitable eyepiece adapters are available for image documentation using:
−the SONY cameras "Digital Handycam DCR-PC100", "Digital Still Camera Cybershot DSC-F505" and
"Digital Still Camera Cybershot DSC- S70"
−35 mm SLR cameras
−still/video and video cameras with C-mount connector
−video cameras for insertion in the eyepiece, e.g. from L.O.T.-Oriel GmbH; SCI-EYE, Inc.
−microhead video cameras (head diameter: 17 mm) in the JAI-M2200 series
☞The adaptation of other digital cameras with M37 or M52 objective / filter thread must be
tested, as described in section 3.2.3.2, page 3-12!

INTRODUCTION
Carl Zeiss Stemi DV4 / DR
1-4 B 40-003 e 08/00

DESCRIPTION
Stemi DV4 / DR Stemi DV4 / DR product family Carl Zeiss
B 40-003 e 08/00 2-1
2DESCRIPTION:
2.1 Stemi DV4 / DR product family
The product family of Stemi DV4 / DR stereomicroscopes includes the following four microscope bodies:
−Stemi DV4 −Stemi DR1040
−Stemi DV4 SPOT −Stemi DR1663
The Stemis DV4, DR 1040 and DR 1063 are mainly
intended for use with stand C and its accessories
(compact version). Of course, use within the Zeiss
Stemi modular system is also possible.
The Stemi DV4 SPOT has mainly been designed for
use in the Zeiss Stemi modular system. It has
therefore been equipped with a direct connection
facility for the Schott KL 200 cold-light source via
light guides. Of course, the Stemi DV4 SPOT can
also be used with stand C. However, this is barely
useful, since stand C is already equipped with
reflected-light illumination.
The four microscope bodies differ in the following
features:
Stemi DV4
−Continuous magnification settings from 8x for
an overall view to 32x for object details via
change knob (without interchangeable optical
components)
Stemi DV4 SPOT
−Continuous magnification settings from 8x for
an overall view to 32x for object details via
change knob (without interchangeable optical
components)
−Integrated SPOT illuminator for coaxial reflected
light with connector for Schott KL 200 cold-
light source.
Stemi DR 1040
−Magnification setting to 10x for an overall view
or 40x for details via change knob (without
interchangeable optical components).
Stemi DR 1663
−Magnification setting to 16x for an overall view
or 63x for details via change knob (without
interchangeable optical components).
Fig. 2-1 Stemi DV4 / DR microscope body

DESCRIPTION
Carl Zeiss Compact version with stand C Stemi DV4 / DR
2-2 B 40-003 e 08/00
2.2 Compact version with stand C
2.2.1 System diagram
Fig. 2-2 System diagram of compact version with stand C

DESCRIPTION
Stemi DV4 / DR Compact version with stand C Carl Zeiss
B 40-003 e 08/00 2-3
2.2.2 Interfaces of the compact version
Interfaces of the Stemi DV4 / DR with stand C
Mount for reticles dia. 19 mm
Mount dia. 76 mm for microscope body
Mount dia. 53 mm for accessories
Internal thread M50x0.75 for mount for
attachment systems, dust cover,
or annular light adapter
Mount dia. 84 mm for stages,
insert plate
Camera tripod connector 3/8", e.g. for
mobile tripod
2.2.3 Mobile equipment C for Stemi DV4, Stemi DR
Fig. 2-3 Interfaces of Stemi DV4 / DR with
stand C (schematic)

DESCRIPTION
Carl Zeiss Compact version with stand C Stemi DV4 / DR
2-4 B 40-003 e 08/00
2.2.4 Technical Data
Dimensions (width x depth x height)
Stemi DV4 / DR......................................................................................... 230 mm x 300 mm x 350 mm
Weight
Stemi DV4 (microscope body)........................................................................................................1.5 kg
Stemi DV4 SPOT (microscope body) ...............................................................................................1.5 kg
Stemi DR 1040 (microscope body)..................................................................................................1.5 kg
Stemi DR 1663 (microscope body)..................................................................................................1.5 kg
Stand C.........................................................................................................................................3.5 kg
Ambient conditions
Storage and transport (in packaging):
Permissible ambient temperature...................................................................................... -10 to +60 °C
Permissible relative humidity............................................................................ 20 % to 85 % at +35 °C
Atmospheric pressure............................................................................................ 800 hPa to 1060 hPa
Operation
Permissible ambient temperature..................................................................................... +10 to +40 °C
Permissible relative humidity............................................................................................ maximal 85 %
Atmospheric pressure............................................................................................ 800 hPa to 1060 hPa
Pollution degree................................................................................................................................... 2
Operating data
Protection class..................................................................................................................................... II
Protection type............................................................................................................................... IP 30
Electrical safety................................................................ in compliance with DIN EN 61010 (IEC 1010-1)
including CSA, UL, ENEC, Q and MITI regulations
Excess voltage category........................................................................................................................ II
Radio interference suppression...................................................... in accordance with EN 55011, Class B
Insensitivity to noise................................................................................ in accordance with EN 50082-2
Line voltage............................................................................................................ 100 to 240 V ±10 %
Change of line voltage is not required!
Line frequency..................................................................................................................... 50 to 60 Hz
Power consumption...................................................................................................................... 30 VA

DESCRIPTION
Stemi DV4 / DR Compact version with stand C Carl Zeiss
B 40-003 e 08/00 2-5
Optical Data
Stemi DV4 and Stemi DV 4 SPOT
Eyepiece W 10x/20 Br. foc.
Attachment systems 0.3x 0.4x O.3x...0.5x 0.63x without 1.25x 1.6x 2x
FWD in mm 285 210 245...85 130 92 60 48 29
Magnification 2.4x...9.6x 3.2x...12.8x 2.4x...16.0x 5.0x...20.2x 8.0x...32.0x 10.0x...40.0x 12.8x...51.2x 16.0x...64.0x
Object field in mm 83.3...20.8 62.5..15.6 83.3...12.5 40.0...9.9 25.0...6.3 20.0...5.0 15.6...3.9 12.5...3.1
Stemi DR 1040
Eyepiece W 10x/20 Br. foc.
Attachment systems 0.3x 0.4x O.3x...0.5x 0.63x without 1.25x 1.6x 2x
FWD in mm 285 210 245...85 130 92 60 48 29
Magnification 3.0x/12.0x 4.0x/16.0x 3.0x...5.0x /
12.0x...20.0x 6.3x/25.2x 10.0x/40.0x 12.5x/50.0x 16.0x/64.0x 20.0x/80.0x
Object field in mm 66.7/16.7 50.0/12.5 66.7...40.0 /
16.7...10.0 31.8/7.9 20.0/5.0 16.0/4.0 12.5/3.1 10.0/2.5
Stemi DR 1663
Eyepiece W 10x/20 Br. foc.
Attachment systems 0.3x 0.4x O.3x...0.5x 0.63x without 1.25x 1.6x 2x
FWD in mm 285 210 245...85 130 92 60 48 29
Magnification 4.8x/18.9x 6.4x/25.2x 4.8x...8.0x /
18.9x...31.5x 10.1x/39.7x 16.0x/63.0x 20.0x/78.8x 25.6x/100.8x 32.0x/126.0x
Object field in mm 41.7/10.6 31.3/7.9 41.7...25.0 /
10.6...6.3 19.8/5.0 12.5/3.2 10.0/2.5 7.8/2.0 6.3/1.6
Illumination
Stemi DV4 / DR
Halogen –integrated reflected-light illuminator .....................................................................12 V, 20 W
Halogen –integrated transmitted-light illuminator..................................................................12 V, 10 W
Stemi DV4 SPOT
Near-axis, inclined reflected-light illumination...............via light guide and Schott KL200 cold-light source
...................................................................................................................................(5.5°illumination)
Plug-in power unit
Protection class .................................................................................................................................... II

DESCRIPTION
Carl Zeiss Zeiss Stemi modular system Stemi DV4 / DR
2-6 B 40-003 e 08/00
2.3 Modular Zeiss Stemi system
2.3.1 System diagram (extract)
Fig. 2-4 Stemi DV4 / DR system diagram within the Zeiss Stemi modular system

DESCRIPTION
Stemi DV4 / DR Zeiss Stemi modular system Carl Zeiss
B 40-003 e 08/00 2-7
2.3.2 Interfaces in the modular system
Interfaces of the Stemi DV4 / DR with column-
type stand
Mount for dia. 19 mm reticles
Mount dia. 76 mm for microscope body
Mount dia. 53 mm for accessories
Internal thread M50x0.75 for mount for
attachment systems, dust cover or
annular light adapter
Mount dia. 84 mm
for stages, insert plates
Distance column –optical axis: 155 mm
Column dia. 32 mm
Mount for integrated illuminator
Fig. 2-6 Front lenses of Stemi DV4 / DR
Fig. 2-5 Interfaces of the Stemi DV4 / DR
with column-type stand (schematic)

DESCRIPTION
Carl Zeiss Stemi DV4 / DR
2-8 B 40-003 e 08/00

OPERATION
Stemi DV4 / DR Basic instrument as compact version with stand C Carl Zeiss
B 40-003 e 08/00 3-1
3OPERATION
3.1 Basic instrument as compact version with stand C
3.1.1 Start-up
•Open the packaging on the top.
•Pull the Stemi DV4 / DR and the expanded
plastic inset out of the packaging in upward
direction and place it on the table.
•Remove the semi-shell which is pointing
upwards.
•Check whether all the components listed in the
delivery note are included in the package.
•Properly dispose of the packaging or keep it for
later reuse.
•Place stand base plate of the stereomicroscope
on a flat, stable support.
•Remove small cardboard box containing the
power supply unit and unpack it.
•Insert primary adapter (corresponding to the
local line voltage) into the appropriate opening
of the power unit until stop.
☞Voltage change on the power unit is
not required. It is automatically
matched to the available line voltage.
•Insert connector of the power unit into the
12 V socket in the rear left of the stand base
plate.
•Connect power unit directly to the line.
The blue power pilot lamp (LED) of the illumination control on the right of the stand base plate lights up.
The instrument is in the standby mode.
☞The packaging cover can be used as dust cover.
Fig. 3-1 Installation and mains connection
of the Stemi DV4 / DR

OPERATION
Carl Zeiss Basic instrument as compact version with stand C Stemi DV4 / DR
3-2 B 40-003 e 08/00
3.1.2 Operation and function controls
1 Connector for changeable optical components and accessories (outer mount dia.: 53 mm, internal thread M50x0.75)
2 Eyepieces W-PL 10x/20 Br. foc., screwed-in, with folding eyecups
3 Carrier handle
4 Magnification changing knob with engraved numbers indicating the set overall magnification
(basic configuration without changeable optical components)
5 Focusing knob to enable focusing of the object
6 Illumination control (see detailed description on page 3-3)
7 Integrated reflected-light illuminator, 12 V / 20 W
8 Clamping screw (socket head SW 3 mm) to fix the microscope body in stand C
9 Integrated transmitted-light illuminator, 12 V / 10 W
10 Insert plate (dia. 84 mm) for object mounting
11 Ball-headed screwdriver (SW 3 mm) in the stand base
Fig. 3-2 Operation and function controls on the Stemi DV4 / DR