Zeiss Primovert User manual

Primovert
Operating Manual

ZEISS Copyright Primovert
2 415510-7244-001 12/2014
Knowledge of this manual is required for the operation of the instrument. Would you therefore please
make yourself familiar with the contents of this manual and pay special attention to instructions
concerning the safe handling of the instrument.
Subject to alterations in the interest of technical progress. The manual is not covered by an update
service.
© Unless expressly authorized, forwarding and duplication of this document, as well as utilization and
communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
Issued by: Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
microscop[email protected]m
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen, Germany
Manual number: 415510-7244-001
Date of issue: Version 12, 12/10/2014

Primovert Contents ZEISS
12/2014 415510-7244-001 3
CONTENTS
Page
1Notes on Instrument Safety ............................................................................................5
1.1 General Safety Notes ..........................................................................................................5
1.2 Instrument Safety and EMC................................................................................................. 5
1.3 Unpacking, Setup, Transportation, Storage.......................................................................... 6
1.4 Disposal.............................................................................................................................. 6
1.5 Operation...........................................................................................................................6
1.6 Warranty Notes ..................................................................................................................8
1.7 Warning and Information Labels..........................................................................................9
2Description ..................................................................................................................... 10
2.1 System Overview ..............................................................................................................10
2.2 Intended Use .................................................................................................................... 12
2.3 Instrument Description and Main Features ......................................................................... 12
2.4 Objectives......................................................................................................................... 14
3Start-Up and Operation................................................................................................. 15
3.1 Starting up the Microscope ............................................................................................... 15
3.1.1 Setting up the Microscope ................................................................................................ 15
3.1.2 Screwing in the Objectives ................................................................................................ 15
3.1.3 Inserting the Condenser.................................................................................................... 16
3.1.4 Inserting the Filter Slider.................................................................................................... 16
3.1.5 Inserting the Phase Contrast Slider .................................................................................... 17
3.1.6 Inserting the Stage Inserts and Attaching the Stage Enlargement Plates .............................. 17
3.1.7 Mounting the Specimen Guide and Inserting the Mounting Frame ..................................... 18
3.1.8 Mounting UV Protection Plate onto Primovert iLED ............................................................ 19
3.1.9 Transmitted-Light Illuminator: Inserting the LED ................................................................. 20
3.1.10 Connecting the Stand with Binocular Tube or Phototube to the Power Supply System ........ 20
3.2 Operating the Microscope................................................................................................. 21
3.2.1 Operational and Functional Elements of the Primovert Microscope ..................................... 21
3.3 Primovert HDcam.............................................................................................................. 27
3.3.1 Control and Functional Elements of the Integrated Camera................................................ 27
3.3.2 Connect iPad to USB charge port ...................................................................................... 29
3.3.3 Ports and their Possible Uses ............................................................................................. 30
3.3.4 Remote Control of Integrated Camera.............................................................................. 34
3.3.5 Configuring the Integrated HD IP Camera.......................................................................... 35
3.4 Switching the Microscope On / Off.................................................................................... 39
3.5 Adjusting Interpupillary Distance and Viewing Height......................................................... 40
3.6 Compensating Defective Vision at the Eyepieces and Inserting the Eyepiece Pointer or
Eyepiece Micrometer......................................................................................................... 41
3.7 Adjusting Transmitted-Light Phase Contrast....................................................................... 42
3.8 Reflected-Light Fluorescence ............................................................................................. 43
3.9 Retrofitting the Microscope............................................................................................... 43
3.9.1 Replacing the Halogen Bulb 6 V / 30 W of the Transmitted-Light Illuminator or the
Transmitted-Light Illuminator with LED .............................................................................. 43

ZEISS Copyright Primovert
4 415510-7244-001 12/2014
3.9.2 Attaching a Camera to the Stand with Binocular Phototube ...............................................44
3.10 Care, Maintenance and Troubleshooting ...........................................................................46
3.11 Instrument Care................................................................................................................46
3.12 Troubleshooting on the Microcope....................................................................................47
3.13 Troubleshooting on the Integrated HD IP Camera ..............................................................49
4Technical Data................................................................................................................51

Primovert Notes on Instrument Safety ZEISS
12/2014 415510-7244-001 5
1NOTES ON INSTRUMENT SAFETY
1.1 General Safety Notes
Please read this operating manual carefully before starting up the microscope.
If you need supplementary information, contact the Carl Zeiss Service or an authorized agency.
To ensure safe operation and troublefree function of the microscope, strictly observe the precautions and
warnings given in this manual.
They are set off herein as follows:
CAUTION
Hazard to the user of the instrument if safety notes are not observed.
ATTENTION
Hot surface!
ATTENTION
LED risk group 2 according to IEC 62471, LED radiation is emitted.
Do not look into the LED beam. It can be dangerous to the eyes.
ATTENTION
Disconnect the plug-in power unit from line power before opening the microscope!
ATTENTION
This symbol indicates a possible hazard to the instrument or system.
NOTE
Working instructions to be observed when using the microscope.
1.2 Instrument Safety and EMC
The Primovert microscopes have been designed, produced and tested in compliance with the standards
DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101 "Safety requirements for electrical equipment for
measurement, control and laboratory use".
The Primovert microscopes meet the requirements of the EC Directive 98/79/EC Annex 1, RoHS Directive
2011/65/EC and carry the mark.
The instruments have to be disposed of in compliance with the WEEE Directive 2011/65/EU and
RoHS Directive 2012/19/EU.

ZEISS Notes on Instrument Safety Primovert
6 415510-7244-001 12/2014
1.3 Unpacking, Setup, Transportation, Storage
Please observe the following safety notes for unpacking, transportation and storage of the microscope:
−The microscope is supplied packed to commercial standards in a plastic case with outer cardboard
package; use the original packaging for transportation.
−Retain the original packaging for a longer storage of the microscope or its return to the manufacturer.
−When unpacking the equipment, check all components for completeness according to the delivery
note.
−
Keep transport and storage temperatures as specified in the Technical Data.
−Set up the microscope on a stable worktable with solid and smooth tabletop.
While using the microscope and up to about 10 minutes after the use there is risk of burns due
to the hot lamp housing.
1.4 Disposal
Please observe the following safety notes for disposing of the microscope:
Defective microscopes should not be disposed of with household waste; dispose of them in
compliance with the provisions of the law.
The manufacturer of the device is under the legal obligation to take back defective devices.
1.5 Operation
The microscope including its original accessories must not be used for microscopic techniques other than
those described in this operating manual.
Please observe the following safety notes when using the microscope:
The manufacturer cannot assume any liability for other applications, including those of
individual modules or single components. This also applies to any service or repair work that is
not carried out by authorized service personnel. In case of non-compliance, all warranty claims
shall be forfeited.
Do not operate the devices and their accessories included in the delivery in potentially explosive
areas nor in the presence of volatile anesthetics or c
ombustible solvents, such as alcohol,
benzine or similar chemicals.
Dirt and dust may impair the performance of the devices. The devices must therefore be
protected from such influences to the greatest possible extent and covered with the dust cover
wh
en not in use. Before covering the devices always check whether they have been switched
off or have switched off themselves automatically in AUTO-OFF mode (then, the blue power-
on LEDs on the right and left side of the stand are off).

Primovert Notes on Instrument Safety ZEISS
12/2014 415510-7244-001 7
The microscope may only be operated by trained personnel who are aware of possible dangers
involved in microscopy and the particular application concerned. The microscope may only be
operated if set up on a stable, solid, smooth and hardly flammable surface.
The microscope is a high-precision instrument that may be impaired in its performance or even
destroyed when handled improperly.
The microscope is equipped with a plug-in power unit allowing line voltages in the range
between 100 and 240 V ±10%, 50 / 60 Hz, without the need for changing the voltage setting
on the instrument.
The plug-in power unit meets the requirements of protecti
on class II (with protective
insulation). If its casing is damaged, put the plug-
in power unit out of operation. The
microscope may be operated only with the plug-in power unit supplied.
If any protective measures are no longer effective, the device must be taken out of service and
secured against inadvertent operation. Please contact a Zeiss service agency or the
Carl Zeiss Microscopy Service to have the device repaired.
−
Always disconnect the power cable before opening the instrument and changing the bulb
or LED.
−Wait for the bulb to cool down before replacing it and do not leave fingerprints on the new
bulb.
−The instrument may only be opened by instructed specialists or service staff.
−The operation of the instrument in explosion-risk environments is not allowed.
Do not replace detachable power cables by power cables with inadequate specifications. Only
the specified power cables should be used.
The microscope can only safety disconnect from line power, when the plug-in power unit is
disconnected from line power. The rotary knob for switching the microscope off (Fig. 11/19)
switchs only to stand by.
Never look into the light beam - neither with nor without optical instruments, even not if you
simply want to observe the specimen. Your eyes may be damaged in case of non-observance!
When using immersion oil, read in any case the safety data sheet.
Immersion oil irritates the skin. Avoid any contact with skin, eyes and clothing.
After skin contact, wash the oil off with plenty of water and soap.
After eye contact, immediately rinse
the eye with plenty of water for at least five minutes. If
the irritation persists, consult a medical specialist.
Proper disposal of immersion oil: Take care to ensure that immersion oil does not enter surface
water or the sewage system.

ZEISS Notes on Instrument Safety Primovert
8 415510-7244-001 12/2014
The microscope is not equipped with special devices for the protection from corrosive,
potentially infectious, toxic, and radioactive or other substances that may be hazardous to
health. If you handle such substances, be sure to observe all legal requirements, in particular
the relevant national accident prevention regulations.
−
Before transporting the instrument, switch it off and let it cool down (hot surface on the
lamp housing).
−The plug-in power unit must not get in contact with moisture.
Obstruction or covering of ventilation slits may result in heat build-up which may damage the
instrument and in extreme cases cause fire.
Always keep the ventilation slits clear and ensure
that no objects enter the instrument through the ventilation slits.
1.6 Warranty Notes
The Primovert microscopes including their original accessories must not be used for microscopic
techniques other than those described in this operating manual. The manufacturer cannot assume any
liability for other applications.
Please consider the following warranty notes for the microscopes:
−The manufacturer guarantees that the device is free from material or manufacturing defects when
delivered.
−Any defects must be notified to us immediately and steps be taken to minimize damage.
−If notified of such a defect, the manufacturer is obligated to rectify it at its discretion, either by
repairing the instrument or by delivering an intact replacement.
−No guarantee is provided for defects caused by natural wear (wearing parts in particular) and improper
use.
−The instrument manufacturer shall not be liable for damage caused by faulty operation, negligence or
any other tampering with the microscope, particularly the removal or replacement of microscope
components, or the use of accessories from other manufacturers.
Unauthorized tampering with the instrument shall lead to a forfeit of all warranty claims.

Primovert Notes on Instrument Safety ZEISS
12/2014 415510-7244-001 9
1.7 Warning and Information Labels
Fig. 1 Warning and information labels on the Primovert

ZEISS Description Primovert
10 415510-7244-001 12/2014
2DESCRIPTION
2.1 System Overview

Primovert Description ZEISS
12/2014 415510-7244-001 11

ZEISS Description Primovert
12 415510-7244-001 12/2014
2.2 Intended Use
The Primovert microscopes are universally applicable light microscopes of inverted design (inverted
microscopes). They are primarily used to examine cell and tissue cultures as well as sediments in culture
flasks, Petri dishes and microtiter plates.
Typical applications (examples):
Observation of intracellular processes on living cell cultures, cell-cell interactions, motility, growth,
examination of blood and tissue samples from the human body.
When handling hazardous substances, observe the instructions on intended operation, correct use and
statutory safety precautions.
2.3 Instrument Description and Main Features
The Primovert microscopes are inverted transmitted-light microscopes of compact design with a small
footprint.
The user can choose from among five types of stands for the brightfield and phase contrast in
transmitted light microscopic techniques.
−Stand-type Primovert with binocular tube
−Stand-type Primovert Photo with binocular phototube for photo and video documentation
−Stand-type Primovert Ergo with binocular ergotube, continuous angle adjustment from 30° to 60°
−Stand-type Primovert HDcam with integrated HD IP CMOS camera and various interfaces and setting
options
−Stand-type Primovert iLED with integrated LED-fluorescence unit (one channel) and binocular
phototube
Important features of the microscope are:
−Stand equipped with a binocular tube or a binocular phototube (50 % vis, 50 % doc) with an
ergonomically favorable tube angle of 45°. The binocular part can be tilted upward and downward
and adapted to the individual eye distance. As there is an upper and lower binocular part position, the
user can choose between two viewing heights.
−Stand equipped with an ergotube with variable tube angle, continuously tiltable in the range from
30° to 60°; the binocular part can be tilted upward and downward and adapted to the individual eye
distance.
−Illumination either by a 6 V / 30 W halogen lamp insert or an LED.
−Continuously adjustable illumination intensity.
−Blue light-intensity indicators installed on both sides, which are visible from a distance.
−External plug-in power unit with cable (incl. cable with multiple plug and country-specific plug inserts).
−Plastic-coated carrying handle integrated in the stand for setting up, demounting and transporting the
device.
−Convenient coaxial coarse and fine focusing drive; adjustable torque of coarse focusing drive.
−Fixed specimen stage suitable for inserting metal or glass plates and attaching the stage enlargement
plates and the specimen guide.

Primovert Description ZEISS
12/2014 415510-7244-001 13
−Quadruple nosepiece with W 0.8" lens thread running on ball bearing.
−Infinity-corrected "Plan-ACHROMAT" objectives with magnifications of 4x and 10x for brightfield and
phase contrast, as well as LD "Plan-ACHROMAT" objectives with long working distance and
magnifications of 20x and 40x for brightfield and phase contrast.
−Preadjusted phase contrast: Use of only one phase stop for objectives with magnifications of 10x, 20x
and 40x for phase contrast.
−Adjustable eyepieces 10x for field-of-view number 20, suitable for spectacle wearers.

ZEISS Description Primovert
14 415510-7244-001 12/2014
2.4 Objectives
The objectives are the optical heart of the microscope. The objectives may be labeled as follows:
LD Plan-ACHROMAT 40x/0.5 Ph 1 ∞/1.0
Where:
LD Long working distance
40x Objective magnification,
with a defined color ring on the
objective being assigned to each
magnification step (Carl Zeiss color code)
0.5 Numerical aperture
∞Infinite mechanical tube length
1.0 Usable with cover glass thickness
D = 1.0 mm, similar data for other glass
cover thicknesses in millimeters
or
0 Usable without cover glass
−Usable with cover glass thickness D = 0 or
0.17 mm
Other labels:
Ph Phase contrast objective with green
inscription
The total visual magnification of the stand with tube / phototube is obtained by multiplying objective
magnification by eyepiece magnification, e.g., 40 x 10 = 400x.
Numerical aperture multiplied by 1000, e.g. 0.5 x 1000 = 500x, presents the maximum useful
magnification; there is no resolution for further details above that limit.
If glass cover slips are employed, objectives can also be used with other cover glass thicknesses.
Available objectives:
Objective Magnification NA Description Working
distance
(mm)
Order No. Field of
view
(mm)
Plan-Achromat 4x 0.10 HF 12 415500-1600-001 20
Plan-Achromat 4x 0.10 Ph0 12 415500-1619-000 20
Plan-Achromat 10x 0.25 Ph1 4.4 415500-1605-001 20
LD Plan-Achromat 20x 0.30 Ph1 4.6 415500-1614-000 20
LD Plan-Achromat 40x 0.50 Ph1 2.8 415500-1617-000 20
LD Plan-Achromat 20x 0.30 Ph2 4.6 415500-1618-000 20
LD Plan-Achromat 40x 0.50 Ph2 2.8 415500-1616-000 20
Fig. 2 Objective (mounting position)

Primovert Start-Up and Operation ZEISS
12/2014 415510-7244-001 15
3START-UP AND OPERATION
3.1 Starting up the Microscope
3.1.1 Setting up the Microscope
Before installing and starting up the microscope, be sure to carefully read and subsequently
observe the notes on instrument safety (see Section 1).
The microscope is supplied completely assembled and, inclusive of its accessories, packed to commercial
standards.
The accessories and individual microscope components (such as sliders, filters or phase stops, specimen
holders, specimen guides or the stage enlargement plates) are delivered in separate packages and must
still be mounted to the microscope.
•Take the microscope out of the transport case and put it on the worktable, using the handle on the
back of the stand. Do not hold the microscope on the front side by the eyepiece part, but by the neck
of the stand between the eyepiece part and the stand base or at the front and at the back of the
stand base.
Retain the original packaging for a longer storage of the microscope or its return to the
manufacturer.
3.1.2 Screwing in the Objectives
•Remove the dust caps from the location holes
of the nosepiece (Fig. 3/2).
•Screw the objectives (Fig. 3/1) in ascending
order into the location holes until the stop is
reached.
•Affix the supplied stickers (Fig. 3/3) indicating
the objective magnification, to the
corresponding positions of the nosepiece.
Recommendation:
Affix
the stickers so that you can read
from the left side (Fig. 3/3
) the
magnification of the objective just
positioned in the light path.
Fig. 3 Screwing in the objectives

ZEISS Start-Up and Operation Primovert
16 415510-7244-001 12/2014
3.1.3 Inserting the Condenser
•Remove the condenser cap.
•Insert the condenser (Fig. 4/4) from the front
into the guide (Fig. 4/3) and push it in to stop.
•Lock the condenser by tightening the socket
head cap screw or the knurled thumb screw
(Fig. 4/1), which is equally supplied with the
microscope.
Recommendation:
If condensers are frequently exchanged
or whenever larger glass vessels are to
be used frequently without a
condenser, the
knurled thumb screw
supplied rather than the socket head
cap screw (Fig. 4/2)
should be
employed to lock the condenser.
3.1.4 Inserting the Filter Slider
•Put the color filter (Fig. 5/1) or the green
interference filter (diameter d=45 mm) into the
opening of the filter slider.
As the filters are clamped in the filter
slider only slightly, not twist-proof, the
filter slid
er should not be turned or
tilted in order to avoid that the filters
fall out and get damaged.
•Push the 2-position filter slider (Fig. 5/2) from
the left or the right side into the slot (Fig. 5/3)
of the upper part of the stand until it engages
in the desired position.
When using the Primovert iLED in
fluorescent mode, use the yellow filter
inserted into the filter slider (included
in the delivery).
It prevents scattered
fluorescent background generated by
unwanted transmitted LED source
phosphor.
Fig. 4 Inserting the condenser
Fig. 5 Inserting the filter slider

Primovert Start-Up and Operation ZEISS
12/2014 415510-7244-001 17
3.1.5 Inserting the Phase Contrast Slider
The 2-position Ph slider (Fig. 6/1) and the
3-position Ph slider (Fig. 6/2) are delivered
completely, with phase contrast stops mounted.
•Push the Ph slider (Fig. 6/1 or 2) from the right
or the left side, with the inscription facing
upwards, into the condenser slot (Fig. 6/3) until
it engages in the desired position.
If necessary, the neutral filter can be
inserted into the free position of the
corresponding phase contrast slider.
This prevents the user from being
dazzled when the slider position is
changed.
3.1.6 Inserting the Stage Inserts and
Attaching the Stage Enlargement
Plates
•Insert either the metal insert (Fig. 7/5) or the
glass insert (Fig. 7/6) into the specimen stage.
The metal insert with a bean-like opening is to be
used to prevent an objective with short working
distance (e.g. 40x) from colliding with the sample
container when being rotated into the light path.
Because of the longer working distance, LD
objectives (LD = long distance) can also be used.
The stage insert with bean-like opening is provided
with an orientation pin to fix its position.
•If the size of the stage surface does not suffice,
stage enlargement plates (Fig. 7/1and 3) can be
attached to both the left and the right side. The
stage enlargement plates have to be attached
to the stage (Fig. 7/4) from below and fixed
using the two knurled screws (Fig. 7/2) included
in the delivery.
If the specimen guide is used, it is not possible to use the stage enlargement plate on the
right side of the stage.
Fig. 6 Inserting the phase contrast slider
Fig. 7 Inserting the stage inserts and
attaching the stage enlargement
plates

ZEISS Start-Up and Operation Primovert
18 415510-7244-001 12/2014
3.1.7 Mounting the Specimen Guide and
Inserting the Mounting Frame
•If attached, remove the stage enlargement plate
from the right side of the stage.
•Attach the specimen guide (Fig. 8/2) from
below to the right side of the stage (Fig. 8/1)
and fasten it with the two knurled screws
(Fig. 8/3) of the specimen guide.
•Insert the universal mounting frame M or the
mounting frame for 96-position microtiter
plates (Fig. 8/5) into the specimen guide,
depending on the sample vessel used. Make
sure that the mounting frame stops properly in
the two fixing clips (Fig. 8/4).
•For Petri dishes with 65 mm or 54 mm
diameter, insert the corresponding mounting
frame into the mounting frame for microtiter
plates.
If required, the mounting frame for Petri dishes d=35
mm can be inserted additionally into
the mounting frame for Petri dishes d=65 mm.
Fig. 8 Mounting the specimen guide and
inserting the mounting frame

Primovert Start-Up and Operation ZEISS
12/2014 415510-7244-001 19
3.1.8 Mounting UV Protection Plate onto
Primovert iLED
•Put the UV protection plate (Fig. 9/1) on the
Primovert fluorescence stand.
•Fix it with the screws (Fig. 9/2) on both sides.
1UV protection plate
2Screws
Fig. 9 Mounting UV protection plate onto
Primovert iLED

ZEISS Start-Up and Operation Primovert
20 415510-7244-001 12/2014
3.1.9 Transmitted-Light Illuminator: Inserting the LED
The microscope is delivered with the transmitted-light illuminator with halogen bulb 6 V 30 W/LED 3 W
already mounted.
If the transmitted-light illuminator with LED (halogen bulb) is to be additionally employed, it must be
inserted into the stand instead of the transmitted-light illuminator with halogen bulb (LED)
(see Section 3.9.1).
3.1.10 Connecting the Stand with
Binocular Tube or Phototube to the
Power Supply System
•If necessary, replace the power supply adapter
attached to the plug-in power unit (Fig. 10/1)
by one of the country-specific adapters supplied
(Fig. 10/3), pulling off the existing adapter and
inserting the desired one.
•Insert the plug (Fig. 10/4) of the plug-in power
unit into the connection socket located on the
rear of the stand (Fig. 10/5).
•Connect the plug-in power unit (Fig. 10/1) with
a power outlet.
•If the plug-in power unit cannot be plugged
into the chosen power outlet because of limited
space, replace the power outlet adapter by the
supplied IEC adapter (Fig. 10/2). This allows the
plug-in power unit to be put flat onto the
tabletop and connected to the power outlet
through a country-specific appliance cable.
Fig. 10 Connecting the plug-in power unit