Zeiss Fluar Illuminator Z mot. User manual

Operating Manual Fluar Illuminator Z mot.
for Axio Zoom.V16

You have purchased a high quality product from Zeiss. Before using
the instrument the first time, please read this operating manual in
order to maintain the high quality of the instrument and ensure
reliable work with it for a long time.

COPYRIGHT
Carl Zeiss Fluar Illuminator Z mot.
4 435180-9060-701 05/2012
Knowledge of this manual is required for the operation of the device. You should therefore familiarize
yourself with the contents of this manual and pay special attention to instructions concerning the safe
operation of the instrument.
We reserve the right to make changes in the interest of technological advancement; the operating
manual is not subject to updating or revision.
© Unless explicitly authorized, dissemination or duplication of this document and commercial
exploitation or communication of its contents are not permitted. Persons in contravention of this
copyright shall be liable to pay compensation for damages.
All rights reserved in the event of a patent grant or registration of a utility patent.
Published by: Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
microscop[email protected]m
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen, Germany
SAP number: 435180-9060-701
Date of issue (Version 5): 01 May 2012

CONTENTS
Fluar Illuminator Z mot. Carl Zeiss
05/2012 435180-9060-701 5
CONTENTS
Page
1INTRODUCTION ........................................................................................................... 6
1.1 General information....................................................................................................... 6
1.2 Notes on instrument safety ............................................................................................ 7
1.3 Notes on warranty ......................................................................................................... 9
2DESCRIPTION ............................................................................................................. 10
2.1 Indication for use ......................................................................................................... 10
2.2 Package check list with accessories............................................................................... 11
2.3 Microscope systems ..................................................................................................... 12
2.4 Technical data.............................................................................................................. 12
3SET-UP ....................................................................................................................... 13
3.1 General information..................................................................................................... 13
3.2 Assemble the Fluar Illuminator Z mot. and connect it up ............................................... 13
3.3 Connecting the HXP 200 C illuminator ......................................................................... 14
3.4 Loading the reflector turret .......................................................................................... 15
3.5 Inserting or removing the stop slider............................................................................. 16
3.6 Connecting the Fluar Illuminator Z mot......................................................................... 17
4OPERATION ............................................................................................................... 18
4.1 Control elements on the Fluar Illuminator Z mot. .......................................................... 18
4.2 Control elements on the HXP 200 C illuminator............................................................ 18
4.3 Switching on and adjusting the Fluar Illuminator Z mot. ................................................ 19
4.4 Switching off the Fluar Illuminator Z mot. ..................................................................... 20
4.5 Wearing parts .............................................................................................................. 21
4.6 Troubleshooting........................................................................................................... 21

INTRODUCTION
Carl Zeiss General information Fluar Illuminator Z mot.
6 435180-9060-701 05/2012
1INTRODUCTION
1.1 General information
Fluar Illuminator Z mot. and the Axio Zoom.V16 microscope have been designed, manufactured and
tested in compliance with DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101 Safety requirements for
electrical equipment for measurement, control and laboratory use.
The Fluar Illuminator Z mot. and Axio Zoom.V16 zoom microscope meet the requirements of EC Directive
IvDD 98/79/EC (In vitro diagnostic medical devices); they are marked with the mark.
The operating manual contains all information and warnings the operator must comply with.
The following warning and information symbols are used in this operating manual:
CAUTION
This symbol indicates a potential hazard to the user.
CAUTION
Optical radiation is emitted. Do not look into the laser beam!
This may be harmful to the eyes (see Section 2.3).
CAUTION
Risk of pinching!
CAUTION
Hot surface!
CAUTION: High-energy UV radiation!
Risk of injury to eyes and skin!
CAUTION
Disconnect the instrument from the power supply before opening!
ATTENTION
This symbol indicates a potential hazard to the instrument or system.
NOTE
This symbol indicates an instruction which requires particular attention.

INTRODUCTION
Fluar Illuminator Z mot. Notes on instrument safety Carl Zeiss
05/2012 435180-9060-701 7
1.2 Notes on instrument safety
Fluar Illuminator Z mot. may only be used for the applications described in this operating
manual. The manufacturer cannot assume any liability for other applications of the instrument,
including those of individual modules or single parts.
Modifications and repairs to this instrument and any devices operated in combination with the
microscop
e must be carried out by our service department or by authorized personnel only. The
manufacturer accepts no liability for damage
caused by unauthorized access to the interior of
the instrument. Failure to comply with this shall also render any warranty claims invalid.
The device may only be operated by instructed personnel. The instruments may only be
operated by trained personnel who are aware of the possible dangers involved with microscopy
and the application concerned. The microscope is a high-pre
cision instrument whose
performance may be impaired if handled improperly.
The separate manufacturer's instructions must be observed and general safety requirements
complied with when connecting and operating the HXP 200 C illuminator.
The HXP 200 C generates high-
energy light with an invisible portion. Risk of dazzling or
blinding! Never look directly into the light beam or light guide exit.
The lamp used in the HXP 200 C becomes very hot during operation and has a high internal
pressure in a heated state. Risk of fire or explosion!
The lamp must be changed in accordance with the manufacturer's instructions.
The illuminator may only be connected to a properly installed earthed power outlet.
The mains plug of instruments with a metal housing (e.g. HXP 200 C) may only be connected
to a socket with earth contact. The protective capacity must not be rendered ineffective by
using an extension cable without a ground wire.
If it is determined that protective measures are no longer effective, the instrument must be
switched off and secured against inadvertent operation. Please contact a Zeiss service agency or
the Carl Zeiss Microscopy Service to repair the instrument.
Dust and dirt may impair the instrument’s performance. The instrument must be effectively
protected from such influences and covered with the dust cover when not in use. Always check
whether the instrument is switched off before you cover it. Broad temperature fluctuations,
direct sunlight and vibrations should be avoided.
The instruments are not equipped with special devices to protect against corrosive, potentially
infectious, toxic, radioactive or other substances that may be hazardous to health. All legal
regulations must be observed when handling such substances, particular
ly the relevant national
accident prevention regulations.

INTRODUCTION
Carl Zeiss Notes on instrument safety Fluar Illuminator Z mot.
8 435180-9060-701 05/2012
On microscopes with motorized focusing drive there is a risk of pinching fingers in the work
area when lowering the body of the microscope.
−Before switching on check that the joystick on the SYCOP 3 is at the zero position and not
swiveled out, and that the foot pedal is clear.
−
Automatic travel can be interrupted by pressing the STOP button on the focusing drive or
the STOP button on the SYCOP 3, moving the joystick on the SYCOP 3 up and down,
turning the knurled wheel on the HIP or pressing the Memory 1 or 2 keys on the HIP.
−Do not reach into the possible travel path of the motorized table. There is a risk of pinching
or injury to the fingers when the table is positioned by the control unit.
Defective instruments are not to be disposed of as ordinary domestic waste. They should be
disposed of in accordance with the relevant regulations.
Samples should also be disposed of in compliance with the relevant legal requirements and
internal operating procedures.
The separate operating manuals for the Axio Zoom.V16, HXP 200 C and SYCOP 3 (if used)
must be observed and the general safety requirements complied with.
−Operating Manual Axio Zoom.V16: Order No. 435080-9030-701, English edition
−Operating Manual SYCOP 3: Order No. 435611-9010-701, English edition
−Operating Manual HXP 200 C
Fig. 1 Warning signs on the Axio Zoom.V16 microscope system with Fluar Illuminator Z mot. and
HXP 200 C illuminator

INTRODUCTION
Fluar Illuminator Z mot. Notes on warranty Carl Zeiss
05/2012 435180-9060-701 9
1.3 Notes on warranty
The manufacturer guarantees that the instrument is free of material or manufacturing defects when
delivered. Possible defects must be notified to us immediately and steps taken to minimize damage. If
notified of such a defect, the manufacturer is obligated to rectify it at his discretion, either by repairing
the instrument or delivering an intact replacement. No guarantee is provided for defects caused by
natural wear (wearing parts in particular) and improper use.
The instrument manufacturer shall not be liable for damage caused by faulty operation, negligence or
any other tampering with the instrument, particularly the removal or replacement of instrument
components, or the use of accessories from other manufacturers. Such actions will render any warranty
claims invalid.
With the exception of the work described in this operating manual, no maintenance or repair work is to
be carried out on these microscopes. Repairs may only be performed by Carl Zeiss service staff or
personnel specifically authorized by Carl Zeiss. In the event of a problem with the instrument, please
contact the Carl Zeiss microscopy service team or your local Carl Zeiss overseas representative.

DESCRIPTION
Carl Zeiss Indication for use Fluar Illuminator Z mot.
10 435180-9060-701 05/2012
2DESCRIPTION
2.1 Indication for use
In conjunction with the HXP 200 C illuminator and filter modules the Fluar Illuminator Z mot. is used to
excite fluorescence in specimens.
The Fluar Illuminator Z mot. is designed exclusively for operation with the Axio Zoom.V16 microscope.
The HXP 200 C illuminator generates extremely high-energy light in the visible and invisible range. The
related safety precautions and instructions for use must be strictly observed (see separate operating
manual).
When operating the microscope always use the anti-glare shield to avoid eye damage due to scattered
light.
The cover on the reflector turret of the Fluar Illuminator Z mot. is provided with safety contacts which
interrupt the beam path to the HXP 200 C when the cover is removed, in order to prevent glare and eye
damage.
The Fluar Illuminator Z mot. cannot be used on the coarse/fine drive with T-column 350 mm.
When operating the Fluar Illuminator Z mot. the separate operating manuals for the Axio Zoom.V16
microscope, HXP 200 C illuminator and SYCOP 3 system control panel (if used) must always be observed.

DESCRIPTION
Fluar Illuminator Z mot. Package check list with accessories Carl Zeiss
05/2012 435180-9060-701 11
2.2 Package check list with accessories
Name/Order No. Figure Remarks
Fluar Illuminator Z mot.
435180-9060-000
with 4x reflector turret and
blind cover of the diaphragm
shaft
Covers with slit for holding
diaphragm slider or
ApoTome.2 slider system
Anti-glare shield
CAN cable RJ 45
1x L = 500 mm
1x L = 1000 mm
Plug-in power unit
Receptacle for reflector
modules
Manual diaphragm slider
423611-0000-000
Motorized diaphragm
slider
423612-9901-000
Choice of reflector
modules:
Z FL P&C ACR
435180-9160-000
Z FL P&C
435180-9260-000
Z FL P&C ACR RW
435180-9260-000
equipped with a choice of
fluorescence filter sets
ACR: automatic component
recognition, readable
ACR RW: automatic
component recognition,
readable and writeable
Name/Order No. Figure Remarks
HXP 200 C illuminator
435716-0000-000
Light generator
CAN cable HD15
457411-9011-000
Country-specific power cable
HXP 200 lamp
000000-0525-316
HXP light guide with
liquid filling, 2 m
000000-0482-760
Warning: Do not bend.
HXP 120 manual/foot
switch
000000-0496-214

DESCRIPTION
Carl Zeiss Microscope systems Fluar Illuminator Z mot.
12 435180-9060-701 05/2012
2.3 Microscope systems
Fig. 2 Axio Zoom.V16 with coarse/fine drive
Axio Zoom.V16 with manual coarse/fine drive
AMicroscope body (Axio Zoom.V16) with
control unit (HIP)
BBinocular photo tube
CEyepiece
DObjective
FIntermediate tube (Fluar Illuminator Z mot.)
GCamera adapter with microscope camera
(AxioCam)
HReflected light illumination (slit-ring light with
cold light source CL 6000 LED)
PStand base with coarse/fine drive
Fig. 3 Axio Zoom.V16 with focusing motor
Axio Zoom.V16 with focusing motor
AMicroscope body (Axio Zoom.V16) with
control unit (HIP)
BBinocular photo tube
CEyepiece
DObjective
ENosepiece
FIntermediate tube (Fluar Illuminator Z mot.)
GCamera adapter with microscope camera
(AxioCam)
HReflected light illumination (slit-ring illuminator
with cold light source CL 6000 LED)
IUV/visual glare shield
KStand base with focusing motor with control unit
(MaRC)
LTransmitted light illumination (transmitted light
illuminator S)
MSpecimen stage (gliding stage)
NSYCOP 3 system control panel
OEMS 3 controller with optional foot pedal S
The items of microscope equipment shown here
are examples and may differ from those
actually provided!
2.4 Technical data
Dimensions
Fluar Illuminator Z mot. (length x width x height) ................................................31 mm x 277 mm x 92 mm
HXP 200 C illuminator (width x height x depth) ............................................... 130 mm x 210 mm x 290 mm
Weight
Fluar Illuminator Z mot. ......................................................................................................................3 kg
HXP 200 C illuminator........................................................................................................................5 kg
Power supply
Fluar Illuminator Z mot. ..............................................................over CAN bus connection from microscope
HXP 200 C illuminator ............................................................................. 100 V AC to 240 V AC, 50/60 Hz
Power consumption
HXP 200 C illuminator ...........................................................................................................max. 320 VA
Mains fuse
HXP 200 C illuminator ....................................................................................................... 2x T4A/H/250V
Ambient conditions
Storage (in packaging)
Permissible ambient temperature.......................................................................................... +10 to +40 °C
Permissible relative humidity ............................................................ max. 75 % at +35 °C (non-condensing)
Transport (in packaging):
Permissible ambient temperature........................................................................................... -40 to +70 °C
Operation
Permissible ambient temperature.......................................................................................... +10 to +40 °C
Permissible relative humidity ..................................................................................................... max. 75 %
Air pressure...............................................................................................................800 hPa to 1060 hPa
Degree of pollution................................................................................................................................ 2
Optical risk group classification acc. to DIN EN 62471:2009
Axio Zoom.V16 microscope system with:
HXP 200 C ............................................................................................... Risk group 1 acc. to DIN EN 62471:2009
CL 9000 LED....................................................................................... LED risk group 1 acc. to DIN EN 62471:2009
Transillumination top 450 mot............................................................ LED risk group 1 acc. to DIN EN 62471:2009
Transillumination base 300 ................................................................. LED risk group 1 acc. to DIN EN 62471:2009
Radiation apertures
Axio Zoom.V16 microscope system with:
HXP 200 C, CL 9000 LED........................................................................... vertically downwards from the objective
Transillumination top 450 mot. ..............................................vertically upwards from the transmitted light adapter
Transillumination base 300........................................................ vertically upwards from the transmitted light base

Set-up
Fluar Illuminator Z mot. General information Carl Zeiss
05/2012 435180-9060-701 13
3SET-UP
3.1 General information
Read the Notes on instrument safety carefully prior to set-up and operation (see Section 1.2,
page 7).
The Fluar Illuminator Z mot. is normally delivered together with the required tools and optional
accessories in several packages.
•Remove all components from the packaging and check that all components described on the delivery
note are present.
•Remove all shipping braces (adhesive tape or similar).
•Keep the original packaging for storage or for returning the instrument to the manufacturer, or
dispose of it properly.
3.2 Assemble the Fluar Illuminator Z mot. and connect it up
In the event that an Axio Zoom.V16 microscope system is not yet available or assembled, the
microscope must initially be installed and
put into operation according to the separate user
manual.
Fig. 4 Fluar Illuminator Z mot. assembly

Set-up
Carl Zeiss Connecting the HXP 200 C illuminator Fluar Illuminator Z mot.
14 435180-9060-701 05/2012
•Switch off the Axio Zoom.V16 microscope and illumination.
•Remove the existing tube (Fig. 4/1) from the body of the microscope by loosening the hexagon socket
screw (Fig. 4/9) with a ball-head screwdriver (SW 3 mm).
•Dismantle the spacer tubes analogously as necessary.
The use of spacer tubes together with the Fluar Illuminator Z mot. is not recommended.
•Remove dust caps from Fluar Illuminator Z mot. (Fig. 4/5).
•Insert the Fluar Illuminator Z mot. into the dovetail mount of the microscope body (Fig. 4/10) and
hand-tighten the hexagon socket screw (Fig. 4/9) with the ball-head screwdriver.
The binocular ergo phototube Z (Fig. 4/1
) should preferably be used for fluorescence
applications with the Fluar Illuminator Z mot.
When the ergo phototube Z is assembled for the first time, the shipping screw (hexagon socket
screw SW 3) on the underside must be screwed out. Keep the shipping screw in a secure place
for use at a later date.
•Insert the tube (Fig. 4/1) into the dovetail mount of the Fluar Illuminator Z mot. (Fig. 4/5) and hand-
tighten the hexagon socket screw (Fig. 4/6) with the ball-head screwdriver.
To prevent eye injury due to scattered light, attach and swing in the anti-glare shield (Fig. 4/7)
on the right or left of the mount or revolving nosepiece (Fig. 4/8).
3.3 Connecting the HXP 200 C illuminator
Never pull the light guide out of the Fluar Illuminator when the HXP 200 C is in operation.
Mount the separately delivered HXP 200 lamp in the HXP 200 C illuminator before connecting
the latter (see manufacturer's instructions).
•Remove the protective caps from each end of the HXP light guide (Fig. 4/3).
•Insert the short end of the HXP light guide (Fig. 4/3) until it engages in the light guide socket on the
reverse side of the Fluar Illuminator Z mot. and secure with clamp screw SW 1 mm (Fig. 4/2).
•Insert the long end of the HXP light guide (Fig. 4/3) into the light guide socket on the reverse side of the
HXP 200 C illumination unit (Fig. 4/4) and push until it engages (locks in automatically).
•Use a power cable to connect the power input on the reverse side of the HXP 200 C illuminator with a
mains outlet.

Set-up
Fluar Illuminator Z mot. Loading the reflector turret Carl Zeiss
05/2012 435180-9060-701 15
3.4 Loading the reflector turret
The reflector turret has four numbered positions,
each for mounting a Z FL reflector module
equipped with the factory-installed desired filter
sets for fluorescence excitation.
For applications without fluorescence one position
should be left vacant.
Attention: Risk of blinding!
The access opening to the reflector turret is located
on the right-hand side of the Fluar Illuminator Z mot.
•Remove the cover (Fig. 5/4) of the reflector
turret from the Fluar Illuminator Z mot. towards
the right.
The reflector turret cover is fitted with safety contacts which are immediately interrupted when
the beam to the HXP 200 C is disconnected, if the latter is switched on.
•Swing the reflector turret into the access opening by turning the manually to the desired position (see
Fig. 5). The position number is marked on the right-hand side of the module mount (Fig. 5/3).
•To install or remove reflector modules (Fig. 5/5) proceed as follows.
Installing reflector modules
•Push the reflector module (slightly tilted towards turret) with holding brackets mounted right and left
(Fig. 5/7) beneath the upper retaining clamps (Fig. 5/2) of the reflector turret position.
•Push reflector module downwards until it locks securely into the lower retaining clamps (Fig. 5/1) .
Removing reflector modules
•Reach below the reflector module and push it horizontally out of the locking position of the lower
retaining clamp.
•Pull the slightly tilted reflector module downwards out of the upper retaining clamps.
Reflector modules with automatic component recognition are equipped with readable (ACR) or
readable/rewritable (ACR RW) Eproms (Fig. 5/6) on
which the installed fluorescence filter
combination is or can be stored. If a SYCOP is connected to the system, the filter combination
for the equipped reflector turret positions is automatically read and imported into the firmware
as a fluorescence configuration.
Fig. 5 Insert Z FL reflector modules

Set-up
Carl Zeiss Inserting or removing the stop slider Fluar Illuminator Z mot.
16 435180-9060-701 05/2012
•Once the configuration of the reflector turret has been completed, replace the cover (Fig. 5/4). To do
this, insert it into the dovetail guides on the underside of the Fluar Illuminator Z mot. and push until it
engages. Make sure it has a precise fit, so that the safety contacts are closed.
•If reflector modules without automatic component recognition are used, apply the supplied stickers
marked with the fluorescence filter combination used to the fields provided beneath the
corresponding control key (Fig. 6/1) and configure as necessary in SYCOP 3.
3.5 Inserting or removing the stop slider
The stop slider is supplied in a manual or motorized version.
Both types are inserted in the Fluar Illuminator Z mot. in the same way. However, the procedure for
removal is different.
The mounting bay for the diaphragm slider is located on the left-hand side of the Fluar Illuminator Z mot.
Inserting the stop slider
•Remove the blind cover (Fig. 6/6) from the
diaphragm shaft of the Fluar Illuminator Z mot. To
do this, press the two latching nipples (top and
bottom) together in the direction of the arrow
and remove the blind cover.
•Insert the slotted cover (Fig. 6/3) instead of the
blind cover. To do this, first insert it into the
recess on the Fluar Illuminator Z mot. with the
latching nipple (Fig. 6/2) on the upper side and
press the underside carefully until the lower
latching nipple engages. Exert only slight
pressure to prevent the cover from being
pushed in too far and becoming jammed.
•Insert the stop slider (manual (Fig. 6/4) or
motorized (Fig. 6/5)) into the slit and push it until
it engages. The locking element of the stop slider
will point downwards.
Fig. 6 Inserting the stop slider

Set-up
Fluar Illuminator Z mot. Connecting the Fluar Illuminator Z mot. Carl Zeiss
05/2012 435180-9060-701 17
Removal of the manual stop slider
•Insert the ball-head screwdriver (SW 3 mm) into
the hole (Fig. 7/2) and push its indentation
upwards into the groove.
•Remove the stop slider with the ball-head
screwdriver.
Removal of the motorized stop slider
•Insert the ball-head screwdriver into the hole
(Fig. 7/1) press against the spring element until
the stop slider projects slightly.
•Remove the stop slider by hand.
•If the stop slider is not required, remove the
slotted cover and replace it with the blind cover.
For special fluorescence applications (producing optical cross-
sections through the specimen)
the ApoTome.2 system can be coupled with the Fluar Illuminator Z mot.
For this purpose the diaphragm shaft is provided with a special cover which permits mounting
of the ApoTome.2 slider. This cover is supplied with the ApoTome.2 system if required.
3.6 Connecting the Fluar Illuminator Z mot.
Connecting to a microscope system with coarse/fine drive
•Connect the CAN-RJ45 socket on the reverse side of the Fluar Illuminator Z mot. to the mains outlet
with a plug-in power unit.
Connecting to microscope system with focusing motor or SYCOP 3 / EMS 3
•Connect the Fluar Illuminator Z mot. to an empty CAN socket on the rear side of the focusing motor
using a 0.5 m CAN-RJ45 cable or to the EMS 3 controller using a 1.0 m CAN-RJ45 cable.
Fig. 7 Removing the stop slider

Operation
Carl Zeiss Control elements on the Fluar Illuminator Z mot. Fluar Illuminator Z mot.
18 435180-9060-701 05/2012
4OPERATION
4.1 Control elements on the Fluar
Illuminator Z mot.
Shutter button (Fig. 8/1):
−Opens and closes the internal shutter
−Integral LED lights up when shutter is open
Buttons 1 to 4 (Fig. 8/2):
−Swivel the corresponding reflector turret
position into the beam path
−Integral LED lights up when this position is
swiveled into the beam path
If the cover is removed from the
reflector turret while the device is
switched on, the five buttons on the
control panel will flash. The turret can
only be rotated manually. The shutter
is closed.
4.2 Control elements on the HXP 200 C
illuminator
Intensity control dial (Fig. 9/1):
−Controls light intensity in stages 0; 12.5; 25; 50
and 100 %
Shutter button remote/open (Fig. 9/2):
−Button in pressed position (open): internal
shutter of HXP 200 C is open
−Button in un-pressed position (remote): internal shutter switched by remote control, e.g. via foot
switch connected to the HXP 200 C or a CAN-HD15 cable to the EMS 3 controller.
Hour counter display with reset button (Fig. 9/3):
−Display shows current number of operating hours of the installed HXP 200 lamp.
−The HXP 200 lamp must be replaced after 2000 operating hours and the hour counter reset to zero
(see manufacturer's instructions)
−The Reset button resets the hour counter to zero
Display shutter open (Fig. 9/4):
−Display LED lights up when shutter is open
Fig. 8 Control elements on the
Fluar Illuminator Z mot.
Fig. 9 Control elements on the HXP 200 C
illuminator

Operation
Fluar Illuminator Z mot. Switching on and adjusting the Fluar Illuminator Z mot. Carl Zeiss
05/2012 435180-9060-701 19
Power switch (Fig. 9/5):
−Switches the HXP 200 C on and off
−Integral LED lights up when switched on
For further instructions for the HXP 200 C refer to the manufacturer's instructions
4.3 Switching on and adjusting the
Fluar Illuminator Z mot.
The Fluar Illuminator Z mot. is switched on
together with the Axio Zoom.V16 microscope
when it is connected to the focusing motor or
EMS 3 controller by CAN-RJ45 cable.
If the Fluar Illuminator Z mot. is
supplied by its own separate plug-in
power unit, it is automatically switched
on as soon as the latter is connected to
the main supply.
•Switch the microscope on by pressing on the
HIP.
After switching on, the shutter of the Fluar
Illuminator Z mot. is always closed.
•Completely open the iris diaphragm of the stop
slider (man. or mot.) using the control knob
(Fig. 10/3) or setting wheel (Fig. 10/6) as
required. Check that the diaphragm is centered.
If necessary, these can be centered with the two
adjusting screws (Fig. 10/1and 4) or (Fig. 10/5
and 7).
•Adjust the microscope to the specimen initially without fluorescence excitation according to the
separate operating manual. For this purpose swivel into a position on the reflector turret without
fluorescence filter set.
•Switch the HXP 200 C illuminator on by the Power switch (Fig. 9/5) and select the desired Intensity
(Fig. 9/1) on the control dial.
•Set Shutter open/remote (Fig. 9/2) to the open position.
The Shutter open display (Fig. 9/4) on the HXP 200 C lights up.
Fig. 10 Manual (right) and motorized (left)
shutter sliders

Operation
Carl Zeiss Switching off the Fluar Illuminator Z mot. Fluar Illuminator Z mot.
20 435180-9060-701 05/2012
•If the foot switch for controlling the shutter of the HXP 200 C is connected, set to remote position.
Then open the shutter with the foot switch.
•Swivel the reflector module with desired filter combination into the beam path by pressing the
corresponding button 1to 4(Fig. 8/2) on the reflector turret.
•Press the Shutter button on the Fluar Illuminator Z mot. (Fig. 8/1) to open the internal shutter
(integral LED lights up).
•Select the desired magnification on the microscope and adjust the focus as necessary.
•To protect the specimen or reduce scattered light, the iris diaphragm of the diaphragm slider (if
existing) can be closed as required using the control knob (Fig. 10/2) or setting wheel (Fig. 10/6).
4.4 Switching off the Fluar Illuminator Z mot.
The Fluar Illuminator Z mot. is switched off together with the Axio Zoom.V16 microscope.
•Switch the microscope off by pressing the button on the HIP or pulling the mains plug of the power
unit.
•Switch the HXP 200 C illuminator off by the Power (Fig. 9/5) switch.