Zeiss Lightsheet Z.1 User manual

Lightsheet Z.1
Operating Manual
February 2013
ZEN 2012 (black edition)

Carl Zeiss Copyright Lightsheet Z.1
II 000000-1790-528 02/2013
Knowledge of this manual is required for the operation of the instrument. Would you therefore please
make yourself familiar with the contents of this manual and pay special attention to hints concerning the
safe operation of the instrument.
The specifications are subject to change; the manual is not covered by an update service.
© Unless expressly authorized, forwarding and duplication of this document, and the utilization and
communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
Issued by Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
[e-mail]
www.zeiss.com/microscopy

INTRODUCTION
Lightsheet Z.1 Carl Zeiss
02/2013 000000-1790-528 III
Contents of this Manual
Chapter 1 Hardware
Chapter 2 Sample Preparation
Chapter 3 Quick Guide
Chapter 4 Software Operation
Annex Lightsheet Z.1 - Overview

CHAPTER 1 - HARDWARE
Lightsheet Z.1 Content Carl Zeiss
02/2013 000000-1790-528 1
CHAPTER 1 HARDWARE
CONTENT
Page
1MAINTENANCE AND CLEANING .........................................................................3
1.1 Maintenance of the Liquid Cooling System.................................................................5
2ERGODRIVE OPERATING PANEL .........................................................................6
3USER INTERFACES ...............................................................................................9
3.1 Installation and Deinstallation of the Detection Modules ........................................10
3.2 Adjustment – Detector Recognition ...........................................................................15
3.2.1 Adjustment – Automatic Detector Alignment..................................................................15
3.2.2 Adjustment – Manual Detector Alignment ......................................................................17
3.2.3 Adjust the Grating Focus for the Automatic or Manual Detector Alignment Tool..............19
3.2.4 Cable Connections for the Detection Module "PCO.Edge"..............................................22
3.2.5 Cable Connections for the Detection Module "Standard" ...............................................23
3.3 Assembly of the Sample Chamber .............................................................................24
3.3.1 Assembly of the Sample Chamber Windows ...................................................................24
3.3.2 Assembly of the Sample Chamber Body and the Sample Chamber Dove Tail Slide............27
3.3.3 Insertion of the Drain Connector, Luer-Lock Connectors and Blind Plugs ..........................29
3.3.4 Insertion of Accessories for Incubation ............................................................................30
3.4 Removing and Inserting the Sample Chamber ..........................................................31
3.5 Assembly of the Sample Holder .................................................................................32
3.5.1 Assembly of the Sample Holder for Capillary ...................................................................32
3.5.2 Assembly of Sample Holder for Syringes..........................................................................34
3.6 Inserting and Removing the Sample Holder..............................................................35
3.7 Installation of the Incubation Modules......................................................................37
3.7.1 Heating Components .....................................................................................................37
3.7.2 CO2-Module Lightsheet Z.1.............................................................................................39
3.7.3 Heating Device Humidity S1............................................................................................41
3.7.4 Registration of the incubation modules...........................................................................41
3.8 Switch Incubation ON and OFF...................................................................................43

CHAPTER 1 - HARDWARE
Carl Zeiss Content Lightsheet Z.1
2 000000-1790-528 02/2013
3.9 Illumination and Detection Optics ............................................................................. 43
3.9.1 Removing and Inserting the Illumination Optics Unit ....................................................... 45
3.9.2 Removing and Inserting the Detection Optics Unit........................................................... 46
3.10 Removing and Inserting the Reflector Turret for Emission Selection ...................... 47
3.11 Removing and Inserting the Reflector Turret for Laser Blocking Filter .................... 49
4INDEX................................................................................................................. 52

CHAPTER 1 - HARDWARE
Lightsheet Z.1 Maintenance and Cleaning Carl Zeiss
02/2013 000000-1790-528 3
1Maintenance and Cleaning
The maintenance to be carried out by the customer is limited to cleaning the painted surfaces, replacing
glass windows and rubber seals on the sample chamber, cleaning and disinfecting the system cavity and
sample chamber, temperature sensors and detection optics unit, replenishment of coolants for the
incubation device and liquid cooling of the detection module as necessary, as well as the replacement of
CO2sterile filters for the incubation device.
a) To clean painted surfaces proceed as follows:
•Switch the device off completely and pull the mains plug.
•Make sure that no cleaning fluid is allowed to enter the system.
•Wipe the painted surfaces with a clean cloth moistened with water to which a small amount of
cleaning agent has been added. Do not use a solvent. Dry off with a lint-free cloth.
b) To clean and disinfect the system cavity proceed as follows:
•Switch the device off completely and pull the mains plug.
•Make sure that no cleaning fluid is allowed to enter the system.
•Take care not to touch the front lens of the illumination and detection optics units. Remove these
optics as necessary.
•Remove the sample chamber for cleaning.
•Cleaning the system cavity:
Wipe the inner surfaces of the system cavity with a clean cloth moistened with water to which a
small amount of cleaning agent has been added. Do not use a solvent. Dry off with a lint-free
cloth.
•Disinfecting the system cavity:
Carefully wipe the inner surfaces of the sample chamber with a lint-free cloth soaked in 70%
ethanol or 70% isopropyl. Allow the solution to react for a few seconds. Repeat the procedure
once or twice as necessary. Carefully wipe off the inner surfaces with a lint-free cloth soaked in
distilled water.

CHAPTER 1 - HARDWARE
Carl Zeiss Maintenance and Cleaning Lightsheet Z.1
4 000000-1790-528 02/2013
c) To clean and disinfect the sample chamber proceed as follows:
•Remove the sample chamber and dismantle it into individual parts. Different procedures are required
for cleaning and disinfecting the various parts of the sample chamber.
−The cover glasses must be properly disposed of in the glass waste.
−The steel parts of the chamber must be cleaned and disinfected as follows:
Rinse the parts with distilled water.
Lay the parts in an ultrasonic bath containing distilled water to which a few drops of detergent
have been added.
Then rinse the steel parts thoroughly with distilled water until all detergent residue has been
removed.
The parts may be laid in a disinfecting bath of 70% ethanol (>1 hour) and then thoroughly
rinsed with distilled water.
The steel parts of the sample chamber may also be autoclaved as necessary.
−Sealing rings, hose connectors and blank plugs must be cleaned and disinfected as follows:
These parts may not be autoclaved.
First of all lay these parts in distilled water and then in a 70% ethanol solution for max. one
hour. Rinse them thoroughly with distilled water.
d) To clean and disinfect the temperature sensor proceed as follows:
•Remove the temperature sensor from the sample chamber and loosen the connections to the main
system module Lightsheet Z.1.
•Once removed, thoroughly rinse the temperature sensor with distilled water and then suspend it for
one hour in a 70% ethanol solution. Make sure that the contact plug remains dry. Complete the
cleaning process by rinsing thoroughly with distilled water.

CHAPTER 1 - HARDWARE
Lightsheet Z.1 Maintenance and Cleaning Carl Zeiss
02/2013 000000-1790-528 5
e) To clean and disinfect the detection optics unit proceed as follows:
•Make sure that no cleaning fluid is allowed to enter the system.
•To avoid scratches, do not wipe the front lens of the optics with dry lens paper and lens cloths.
•Remove these optics from the system cavity as necessary.
•Clean the front lens of optical elements according to general recommendations (see "The clean
microscope" www.zeiss.com/microscopy) using an optical cleaning solution (Carl Zeiss optical cleaning
solution: 85% n - hexan, 15% isopropanol. This solution is not sold by Carl Zeiss Microscopy GmbH)
and a suitable lens cleaning paper or high purity cotton wool.
•In addition to the cleaning procedure described above, for special sterility requirements the front lens
area of the detection modules can be cleaned several times with a lint-free lens cloth or lens paper
soaked in 100% ethanol. Here again, observe the general notes on cleaning the optics
("The clean microscope").
1.1 Maintenance of the Liquid Cooling System
If detection modules in your Lightsheet Z.1 are connected to a liquid cooling system, consider that the pH
value of the cooling liquid changes over time.
To prevent corrosion in the cooler of the detection module, the pH value of the cooling liquid must be
checked at least once a year and the liquid replaced as necessary. Observe the directions of the cooling
system manufacturer in the supplied operating manual.
If you have signed a service agreement with Carl Zeiss, our service staff will perform the check as part of
the maintenance procedure.
The safety data sheet for the cooling liquid should also be observed.

CHAPTER 1 - HARDWARE
Carl Zeiss ErgoDrive Operating Panel Lightsheet Z.1
6 000000-1790-528 02/2013
2ErgoDrive Operating Panel
1Z-axis control
2Rotation button (switches 6 to rotation drive)
3Mode button (fine / coarse)
4Axis button (switches 6 to y-axis drive)
5X-axis control
6Y-axis / Rotation control
Fig. 1 ErgoDrive operating panel