Zeiss LSM 880 User manual

880 Table of Contents
880 User guide overview………………………………………………...2-9
Setting up configurations on your own…………………………...10-11
Time Series………………………………………………………………………15
Z-Stacks……………………………………………………………………………16-17
Calibration for Airyscan…………………………………………………….22-25
Acquisition mode-Recommended settings………………........12
Tile Scans and Stitching…………………………………………………...13-14
Adding a DIC Transmitted Light Image……………………………..18
Kohler Illumination…………………………………………………………..19
Incubation………………………………………………………………………..20-21
1

880 User Guide Overview
• Starting up the system
• Turning on the lasers
• Viewing your sample
• Setting up a configuration
• Taking an image
• Saving your file
2

Starting up the system
1. Follow the directions for Powering up the 880, this is located on the wall behind the
880 monitor
2. Double click on the Zen icon
3. Click on the Start System tab
3

Turning on the lasers
1. The system starts with the locate tab open
2. To turn on the lasers click the acquisition tab
3. Use the pulldown tab to turn on the lasers
Note: the 488 laser takes about 5
minutes to warm up.
4

Viewing your sample
1. To view your sample through the eyepiece
click the locate tab
2. Choose your objective by clicking on the objective
button, this will bring up a list of objectives
currently on the microscope.
You may also choose your objective by using the
touch screen next to the monitor – Touch “Home”
then “Microscope” and click on the “Objectives”
Tab.
3. After you choose your objective make sure to
clean it with lens cleaner
4. Set your sample on the stage, please remember
to add oil or water as appropriate
5

Viewing your sample
1. To view your sample through the eyepiece
select what color you would like to use.
If you would like to see white light select DIC
Blue Green Red
2. Use the focus wheels on the side of the
Microscope, or on the side of the touch
screen to bring your sample in focus.
Towards yourself moves the objective down
Away from yourself moves the objective up
3. Use the joystick next to the microscope to move your sample.
6

Setting up a configuration
1. To load a configuration from a previous experiment, go to
file, open, then choose the experiment you would like to
reuse
2. Click the reuse button either on the top left of the screen
or at the bottom of your image
3. Please contact Jeff, Agnes or Erica if you need to set up a new configuration.
7

Taking an image
1. To view your sample click on the Live button.
2. You may need to adjust to focus by using
the focus wheels on the microscope or on the
side of the touch screen.
3. To change the intensity of your sample go to
the channels tab and click on the track you
want to adjust. You can increase or decrease
your intensity by adjusting the Gain(Master)
It is recommended you stay between 600-800
4. You may also need to adjust the laser power.
Please talk to Jeff, Agnes or Erica if you have
questions about selecting the appropriate level
for the laser intensity.
4. Once you have your image in focus and at the intensity you like, click the
Snap button to acquire your data quality scan. 8

Saving your file
1. After you have taken your image you may save your file by using one of the following
options:
-clicking on File, then Save
-clicking on the disk icon on the top left
-clicking on the disk icon on the right, under images and documents
9

Setting up configurations on your own
1. Click on button that looks like an open file folder
it will bring up a menu called
Load Acquisition Parameters
2. Choose what laser lines you would like to use
to excite your sample. Then click on the setup
that contains those lasers.
Note: Examples of commonly used dyes and the
laser lines used to excite them are listed on the
next page.
Note: The files that start 880 are for the regular
confocal and the ones that start AS are for the
Airyscan
10