Agilent Technologies 1100 Series User manual

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Agilent 1100 Series
Handheld Control Module
User’s Guide

Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Germany
Copyright Agilent
Technologies 2000
All rights reserved.
Reproduction, adaption,
or translation without
prior written permission
is prohibited, except as
allowed under the
copyright laws.
Part No. G1323-90006
Edition 01/00,
Printed in Germany
Warranty
The information
contained in this
document is subject to
change without notice.
Agilent Technologies
makes no warranty of
any kind with regard to
this material,
including, but not
limited to, the implied
warranties or
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fitness for a particular
purpose.
Agilent Technologies
shall not be liable for
errors contained herein
or for incidental or
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WARNING
For details of safety,
see Safety Information
on page 142
Warning Symbols Used
In This Book
The apparatus is marked
with this symbol when
the user should refer to
the instruction manual
in order to protect the
apparatus against
damage.
!

User’s Guide
Agilent 1100 Series Handheld Control Module

4
This book describes how to operate the Agilent 1100 Series modules and
systems for HPLC using the control module, shown opposite. The control
module provides complete local control and monitoring of a single module or
an entire Agilent 1100 Series system. There is no data evaluation in the
control module. The control module allows you to do a variety of HPLC tasks
including automated sample preparation and injection, isocratic, gradient
and multiple method analyses.
Other Books Available:
Each HPLC module is supplied with a Reference Manual which describes
installation, troubleshooting, repair, parts and theory of operation.
The control module is supplied with the following:
• User’s Guide
• Software Overview Guide
• Performance Verification Manual
• Online Information System
In This Book

5
In This Book
Solvent cabinet
Vacuum degasser
Pump
Column compartment
Autosampler
Detector
Control module

6
In This Book

7
In This Book
Part 1 “Using the Agilent 1100 Series Control Module”describes the control
module, its features and its functionality.
Part 2 “Using the Agilent 1100 Series Modules”describes how to use the
individual HPLC modules to run isocratic, gradient and multiple-vial analyses
using a single method or more than one method.
Part 3 “Using the Agilent 1100 Series LC System With Control Module”
describes how to run isocratic, gradient and multiple-vial analyses using a
single method or more than one method.

8
In This Book

Contents
9
Part 1 Using the Agilent 1100 Series Control Module
1 The Agilent 1100 Series Control Module
Overview over the Agilent 1100 Series control module 18
Control Module Features 19
Control Module Keys 20
Control Module Software 24
Control Module Versions 31
2 Working with the Control Module
How to use the Agilent 1100 Series control module 33
Installing the Control Module 34
General Functions 35
Troubleshooting 36
Inserting and Removing PC cards 37
Working with Methods 38
Time Programming 43
Automating Analyses 44
Displaying Data Graphically 46
Logbooks 48
Printing Screens 49
Connecting External Devices 51
Firmware 53
Updating Firmware in the Control Module 53
Updating Firmware in the LC Modules 54
Coexecution with Agilent ChemStation 58

10
Contents
Part 2 Using the Agilent 1100 Series Modules
3 Using the Pump
Operational details for the Agilent 1100 Series pumping systems 63
Turning the Pump On and Off 64
Entering Settings 64
Purging the Pump 64
Purging Procedure 65
Adjusting Compressibility 66
Adjusting Stroke Volume 67
Automatic Shut-down 67
Troubleshooting the Pump 68
Tracking Pump History / Pump EMF Limits 68
Resetting the Pump 69
Resetting the Pump Settings 69
4 Using the Degasser
Operational details for the Agilent 1100 Series vacuum degasser 71
Starting the Degasser 72
Removing Gas Bubbles 72
Changing Solvents 73
5 Using the Autosampler
Operational details for the Agilent 1100 Series autosampler 75
Configuring the Autosampler 76

Contents
11
Entering Settings 76
Aligning Transport Arm/Gripper 79
Tracking Autosampler History / EMF limits 79
Resetting the Autosampler 80
Troubleshooting the Autosampler 80
6 Using the Manual Injection Valve
Operational details for the Agilent 1100 Series manual injection
valve 81
About the Injection Valve 82
Making An Injection 82
Completely Filling the Sample Loop 83
Partially Filling the Sample Loop 83
How Much Sample Is Actually Injected? 84
How Much Sample Do I Need? 84
An Alternative Way to Fill The Loop 85
7 Using the Detectors
Operational details for the Agilent 1100 Series variable wavelength,
multiple wavelength, refractive index, fluorescence light, and diode ar-
ray detectors 87
Turning the Lamp On and Off 88
Entering Settings 88
Resetting the Baseline 90
Configuring the Detector 90
Troubleshooting the Detector 91
Tracking Detector History 91
Resetting the Detector 92

12
Contents
Lamp Ignition Routine (VWD only) 92
Configuring the Analog Channel Output 92
8 Using the Column Compartment
Operational details for the Agilent 1100 Series thermostatted column
compartment 95
Turning the Column Compartment On and Off 96
Entering Settings 96
Configuring the Column Compartment 97
Configuring the Column ID Module 97
Selecting Separated or Combined Mode 98
Selecting the Column Switching Valve (optional) 98
Troubleshooting the Column Compartment 99
Tracking Column Compartment History 99
Tracking Column ID History 99
Resetting the Column Compartment 100
Part 3 Using the Agilent 1100 Series LC System With Control Module
9 Running an Isocratic Analysis
How to analyze the Agilent Technologies isocratic standard sample us-
ing a single injection analysis 103
What You Will Need 104
Preparing the LC System 104
Entering Settings 105

Contents
13
Saving Settings in a Method 106
Observing the Chromatogram 107
Starting the Analysis 108
10 Running a Gradient Analysis
How to analyze the Agilent Technologies isocratic standard sample us-
ing a gradient analysis 109
What You Will Need 110
Preparing the LC System 111
Entering Settings 111
Saving Settings in a Method 114
Observing the Chromatogram 114
Starting the Analysis 116
11 Running Multiple-Vial Analyses
How to setup multiple vial analyses using the same method and differ-
ent methods 117
Analyzing Multiple Vials Using the Same Method 118
Analyzing Multiple Vials Using Different Methods 118
Single-Level Calibration Sequences 120
Multiple-Level Calibration Sequences 121
Recalibrating With the Same Group of Standards 121
Recalibrating With Multiple Groups of Standards 123
Synchronizing Analyses with External Devices 126

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Contents
12 Running an Injector Program
How to create an injector program 129
Creating an Injector Program 130
Entering Injector Program Settings 130
Saving the Method 131
Safety Information 142
Radio Interference 144
Agilent Technologies on Internet 145

Part 1
Part 1 Using the Agilent 1100
Series Control Module
1 The Agilent 1100 Series Control Module 17
2 Working with the Control Module 33

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Using the Agilent 1100 Series Control Module

1
1The Agilent 1100
Series Control
Module

18
The Agilent 1100 Series Control Module
Overview over the Agilent 1100 Series control
module

19
The Agilent 1100 Series Control Module
Control Module Features
Control Module Features
The control module provides complete local control and monitoring of a single
module or an entire Agilent 1100 Series system. You have easy access on every
supported function, you can easily control all parameters and settings and you can
configure various communication channels with other devices, in order to
comfortably analyze the generated data.
• Install any desired configuration of Agilent 1100 Series modules. The control
module software will reflect which modules are present in the LC system and
adjust the screens accordingly.
• Enter parameter settings for every module, perform reset and on/off functions as
well as calibration and configuration settings in a self-explanatory and intuitive
way.
• Define automated analyses including methods, timetables, injector programs,
method sequences and automated calibration settings using the control module.
• Protect your method from any inadvertent keyboard changes by setting method
protection.
• Use PC cards to store and transfer methods and sequences between Agilent1100
systems.
• Monitor all operations and error events using the self-updating logbooks.
• Use the context-sensitive online information system to get further information on
all topics.
• Use the context-sensitive menu function to have the quickest access on related
functions.
• To help comply with Good Laboratory Practice (GLP) regulations you can select
a variety of module tests that will check the performance of the LC system.
• The early maintenance feedback (EMF) limits can be used for scheduling
maintenance work.
• Display data graphically using the Plot screen where as many as three different
signals can be monitored at the same time.
• Print information to a PCL3 compatible printer connected to the serial RS232
port of a Agilent1100 module.

20
The Agilent 1100 Series Control Module
Control Module Keys
Control Module Keys
NOTE A short description of the main keys and the product and serial number is located on
the rear of the control module.
Figure 1 The Agilent 1100 Series Control Module
i (info) key
provides
context
specific
information
for current
item selected
Enter key
acceptscurrent
input entry or
action
Selection keys
select settings, values or
alphabetic characters
Direction keys
move the cursor in
the screen display
Navigation keys
access different
software areas
Esc key
reverses current
input entry or
action
Numeric keys
enter numbers 0 to 9
Action keys
execute
specific
operations and
access Plot
screen
m (menu) key
provides
navigational
shortcuts and
actions
Alphanumeric keys
press numbers 1 to 9 multiple times
(e.g. 1 A B C, 2 D E F, 3 G H I ,…)