Agilent Technologies SD-1 User manual

Agilent Technologies
Agilent SD-1 Isocratic
Solvent Delivery Module
User Manual
SD-1 Solvent Delivery - User Manual

SD-1 Solvent Delivery - User Manual
Notices
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Manual Part Number
G9302-90001
Edition
01/2013
Printed in Germany
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SD-1 Solvent Delivery - User Manual 3
In This Book
In This Book
This manual contains information on:
•Agilent SD-1 Isocratic Solvent Delivery Module (G9302A)
•Agilent SD-1 Add-on Solvent Delivery Module (G9303A)
1Introduction
This chapter gives an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3Installation
This chapter gives information about the installation of your instrument.
4 Using the SD-1 Solvent Delivery Module
This chapter explains the operational parameters of the instrument.
5 Optimizing Performance
This chapter gives hints on how to optimize the performance or use additional
devices.
6 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features.
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 SD-1 Solvent Delivery - User Manual
In This Book
8 Maintenance and Repair
This chapter describes the maintenance of the instrument.
9Parts
This chapter provides information on parts for the instrument.
10 Cables
This chapter provides information on cables used with the instrument.
11 Appendix
This chapter provides addition information on safety, legal and web.

SD-1 Solvent Delivery - User Manual 5
Contents
Contents
1Introduction 7
Introduction to the System 8
Physical Layout 9
Check Valves 12
Control 14
2 Site Requirements and Specifications 23
Site Requirements 24
Physical Specifications 27
Performance Specifications 28
3Installation 31
Installation 32
4 Using the SD-1 Solvent Delivery Module 33
Hints for Successful Use of the Pump 34
Introduction 36
Power On 37
Initial Operation 38
Operation 42
5 Optimizing Performance 53
Maximum Pressure versus Flow 54
Scale-Up 55
System Requirements for High Performance Prep Chromatography 56
6 Troubleshooting and Diagnostics 61
Introduction to Troubleshooting and Diagnostics 62
Using the Pressure Display as Diagnostic Tool 63
Troubleshooting Guide 64

6 SD-1 Solvent Delivery - User Manual
Contents
7 Error Information 71
What are Error Messages 72
Pump Error Messages 73
8 Maintenance and Repair 77
Introduction to Maintenance 78
Warnings and Cautions 79
Maintenance Schedule 81
Service Logs 83
Clearing Air Bubbles from the Liquid Head 84
Replacing Seals 86
Replacing Piston 92
Cleaning Check Valves 95
Replacing Check Valves 97
Checking and/or Changing Power Fuses (F1) 99
9Parts 101
Modules 102
Pump Heads 103
Accessories 104
Pump Head Replacement Parts 105
10 Cables 109
Cable Overview 110
Cable Connections 111
11 Appendix 113
General Safety Information 114
Solvent Miscibility 120
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002-96-EC) 121
Batteries Information 122
Radio Interference 123
Electromagnetic Compatibility 124
Agilent Technologies on Internet 126

7
SD-1 Solvent Delivery - User Manual
Agilent Technologies
1
Introduction
Introduction to the System 8
Physical Layout 9
Check Valves 12
Control 14
This chapter gives an instrument overview.

8 SD-1 Solvent Delivery - User Manual
1Introduction
Introduction to the System
Introduction to the System
The Agilent SD-1 is an innovative HPLC solvent delivery system engineered
with preparative chromatographers in mind. Corrosion-resistant titanium
pump heads address the needs of biochemists for compatibility with
salt-containing buffers and freedom from unwanted metal ions. Each pump
head has a piston washing chamber to prevent deposition of abrasive salt
residues behind the high-pressure seal, thereby greatly extending seal life.
Major new technology includes dual independent linear piston drives. Rather
than operating both heads from cams attached to a single motor, as in
conventional dual piston pumps, the Agilent SD-1 uses two independent
stepper motors which connect to pistons via linear screw drives. The motors
reciprocate, rather than running only in one direction. Independent drive
frees the Agilent SD-1 from the operating constraints of mechanical cam
profiles and makes it the first dual piston pump to produce entirely pulse-free
flow under all operating conditions, without auxiliary hydromechanical pulse
dampers. Totally pulse-free flow favors improved column performance and
extended column life by saving expensive preparative columns from the
constant pressure-pulse pounding typical of other large piston and diaphragm
pumps.
In addition, the Agilent SD-1 meters flow at a constant rate independent of
solvent compressibility and without the slight refill-associated flow deficits
seen with conventional single-motor dual-piston models. In gradient
operation, this produces more precise composition profiles without the time
and volume delays introduced by large mixing chambers. Plus, flow rates
extend well into the analytical HPLC range, permitting method development
on smaller columns without interchanging heads.
Each Agilent SD-1 pump includes a standard serial interface for flow control.
A built-in two-channel analog-to-digital converter allows the computer to
collect data from HPLC detectors. Contact closure inputs and outputs are
provided for system automation. Firmware includes Good Laboratory
Practices (GLP) logging features.

SD-1 Solvent Delivery - User Manual 9
Introduction 1
Physical Layout
Physical Layout
Figure 1 Agilent SD-1 Isocratic Solvent Delivery Module – front view
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10 SD-1 Solvent Delivery - User Manual
1Introduction
Physical Layout
Figure 2 Agilent SD-1 Isocratic Solvent Delivery Module – liquid end detail
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SD-1 Solvent Delivery - User Manual 11
Introduction 1
Physical Layout
Figure 3 Agilent SD-1 Isocratic Solvent Delivery Module – rear panel
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Tabl e 1 Rear panel
Component Function
Fan Cooling fan draws cooling air into back of pump, blows warm air out of bottom of pump.
External contacts
panel
Contains three terminal blocks (TB):
•TB-1 Analog
•TB-2 Digital input
•TB-3 Relay output
Overtemperature
reset switch
Safety feature cuts power to the SD-1 in case of overheating. The reset switch pops out. Restore
power to the pump by pressing the reset switch back in place.
Fuse holder Holds two fuses.
Serial connector Miniature D-connector used only for remote pump control by compatible PC software.
Mini-DIN connector 8-pin miniature connector.
Power receptacle Accepts standard grounded AC power cord.

12 SD-1 Solvent Delivery - User Manual
1Introduction
Check Valves
Check Valves
The pump head has one inlet check valve and one outlet check valve.
Figure 4 Cutaway view pump head
Figure 5 Outlet check valve – sectional view
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SD-1 Solvent Delivery - User Manual 13
Introduction 1
Check Valves
Principle of Operation
The retracting piston creates a negative pressure in the piston chamber above
the inlet check valve. Mobile phase flows upward past the check ball into the
inlet check valve, then into the piston chamber.
As soon as the piston starts to move forward, gravity causes the ball in the
inlet check valve to seat, preventing mobile phase from flowing back out the
inlet check valve. At the same time, a positive pressure is created in the piston
chamber which dislodges the outlet check valve check ball. Mobile phase flows
upward through the outlet check valve while the piston is moving forward.
When the piston retracts again, gravity causes the ball in the outlet check
valve to seat, preventing mobile phase flowing back out the outlet check valve,
and the cycle is repeated.
NOTE
During manufacture, each check valve is closely inspected and then individually assembled
in a clean-room. Check valves should be kept clean and in good condition for reliable,
reproducible flow.

14 SD-1 Solvent Delivery - User Manual
1Introduction
Control
Control
Keypad
Figure 6 Keypad

SD-1 Solvent Delivery - User Manual 15
Introduction 1
Control
Cursors
In the Agilent SD-1 displays, parameters which can be edited are indicated by
one of four types of cursor. All types of cursors flash both sides of the
parameter to be edited. Each type of cursor has a specific function.
Tabl e 2 Control keys
Key Function
FLOW FLOW opens the Flow window where the flow rate and ramp time are set. Maximum flow rate
depends on the head size in use; maximum ramp time is 9999.99 min.
PRESS PRESS opens the Pressure window where several pressure conditions can be set: Maximum
pressure, pump action when maximum pressure is reached, minimum pressure, pressure units and
analog pressure signal on/off.
~~opens the Setup and Service Log menus. The Setup menu is used to start the head change
sequence, set the Pump ID, the CIM ID, and to set the internal clock. The Service log is used to log
piston seal changes, check valve changes/service intervals, and show the pump drive status.
UP/DOWN
ARROWS
UP/DOWN ARROWS are used to scroll up or down through preset values or toggle between
choices. The DOWN ARROW key is also used to open menus.
LEFT/RIGHT
ARROWS
LEFT/RIGHT ARROWS are used to move right and left in the display to access adjacent menus or
values.
RUN RUN starts the ramp to the flow rate set in the FLOW window. The ramp time is set in the FLOW
window.
HOLD HOLD keep the ramp at its current position. The time into ramp is displayed, with the flow rate and
current pressure. This key is canceled by pressing RUN.
STOP STOP cuts off the flow immediately.
Numeric keys Used to set numeric values: flow rate, minimum and maximum pressure, ramp time, pump ID, CIM
ID, date and time.
CLEAR CLEAR cancels user-entered value or choice, leaving the previously entered setting intact. Also
used when changing heads.
ENTER ENTER accepts a new value or choice. Also used when changing heads.
Power switch Power switch turns power to the pump on or off. Connected directly to the power module on the
rear panel.

16 SD-1 Solvent Delivery - User Manual
1Introduction
Control
Home Display
The Flow display is also the home display. This display shows when the SD-1
starts up and can be accessed at any time by pressing the FLOW button. The
top line of the display always shows current flow rate and pressures.
Tabl e 3 Specific cursor functions
Cursor Name Function
- - NUMERIC
ENTRY
CURSOR
Used for numeric entry only. Values entered or edited while the cursor is flashing are
temporary until accepted by pressing the ENTER key, the RIGHT ARROW key, the LEFT
ARROW key, or the RUN key. If the edited value is not accepted by pressing one of the
above keys, or cancelled by pressing the CLEAR key, the parameter reverts to its previous
value after 60 seconds.
↓↑ SCROLL
CURSOR
Used when there is a preset list of choices. Pressing the UP ARROW or the DOWN
ARROW with this type of cursor is displayed scrolls up or down through the preset choices.
?? DUAL-MODE
CURSOR
Used when there is a preset list and numeric entry. Used when the value can be set either
by numeric entry or by scrolling through a preset list of choices, as described above.
| | MENU
CURSOR
Used when the selection is a menu. Pressing the DOWN ARROW or the ENTER key with
this type of cursor displays the next level of the menu.
NOTE
The following are not cursors, but are described here for clarity.
* ASTERISK Used to show cursor position (for accessing HELP) when the parameter in question is a
status indicator and cannot be edited.
←→ LEFT-RIGHT
ARROWS
These indicators are used at the right or left edge of the display to indicate that more
information can be seen by pressing either the RIGHT or LEFT ARROW key.

SD-1 Solvent Delivery - User Manual 17
Introduction 1
Control
Figure 7 Home/flow display
&
'
(
)
*
Tabl e 4 Structure of the home display
Area Display
1 This area of the display shows the current state of operation (if any). Time is shown when ramping.
Stop
The pump is stopped because:
•the STOP button has been pressed, or
•a stop signal has been sent from the software, or
•a pressure limit — stop action has been received, or
•zero flow rate has been programmed.
Ramp
The RUN button has been pressed and the pump is ramping to a new value. The elapsed ramp time is also shown in
this area.
Hold
The HOLD button has been pressed and the ramp has been interrupted. The pump continues at the flow rate
reached when the HOLD button was pressed. The HOLD state can be canceled by pressing RUN or STOP.
2 Current flow rate is shown here.
3 Current system pressure is shown here.
4 Ramp time is set here, in minutes decimal format, (for example, 2 min15 s is set as 2.25 ). Ramp time is the time for
a linear change from current to new flow rate.
5Theflowrateissethere.(–xxx.xx—indicates active cursor). Legal flow rates are from zero to the maximum for the
pump head installed (200 , 500 , 800 or 3200 mL/min).

18 SD-1 Solvent Delivery - User Manual
1Introduction
Control
Main Displays
The main displays are accessed by pressing the function buttons on the front
panel.
Use this display to set the flow rate and ramp time. Legal flow rates are from
zero to the maximum for the pump head installed (200 , 500 , 800 or
3200 mL/min). Ramp time (in minutes; decimal format) is the time for a linear
change from the current to the new flow rate. Legal ramp times are from 0.00
to 9999.99 min.
Pressing the PRESS key opens the Pressure display.
Figure 8 Pressure display 1
To reach the rest of this display, press the RIGHT ARROW key (indicated by the
small arrow in the lower right corner). To reach the first part of the display
again, press the LEFT ARROW key.
Figure 9 Pressure display 2
The main displays sets:
•MAXP: Maximum pressure
•ACTION: When maximum pressure is reached limit flow to maintain the
maximum pressure, or press STOP to stop the pump
•MINP: Minimum pressure;

SD-1 Solvent Delivery - User Manual 19
Introduction 1
Control
Pressure below which the pump will stop — must go for about 10 s then stay
below for 0.5 s to stop
•UNITS: Pressure units psi, bar or MPa
•ANALOGPRESSUREMONITOR: ON or OFF;
Sends an analog signal (1000 psi/V) to an external pressure monitor, if the
instrument control software is not controlling the detector wavelength
through the ANALOG OUT contacts on the SD-1.
When controlling detector wavelength, the instrument control software may
override this control. The SD-1 may need to be switched off and on to release
the override.
Special displays
Pressing the ~ key opens the Special display, to access the SETUP and SERVICE
LOG menus.
SETUP Display
Figure 10 Special display
Figure 11 SETUP display

20 SD-1 Solvent Delivery - User Manual
1Introduction
Control
The SETUP menu sets:
•HEADS
•HEADS CHANGE: Start the software-driven procedure to change pump
heads.
•HEADS ID: Display the size of pump head installed and pressure
maximum.
•DEVICE#
•DEVICE# PUMP: Set the ID number for the pump. (When under the control
of instrument control software, each device in the HPLC system must
have a unique ID number.)
•DEVICE# CIM: Set the ID number for the built-in control interface.
•SETCLOCK
•SET CLOCK MM/DD/YY: Set month/day/year with numeric keys.
•SET CLOCK HH:MM:SS: Set hour/minute/second with numeric keys.
SERVICE LOG display
Figure 12 Special display
Figure 13 SERVICE LOG display
The SERVICE LOG menu opens three log displays:
•Piston seal log
•Check valves log
•Pump drive log