Agilent Technologies LC Series User manual

1290 Infinity II Valve Drive and Valve Heads
Agilent InfinityLab LC Series
User Manual
1290 Infinity II Valve Drive and Valve Heads User Manual

1290 Infinity II Valve Drive and Valve Heads User Manual
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Document No: SD-29000412 Rev. C.00
Edition: 06/2021
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1290 Infinity II Valve Drive and Valve Heads User Manual 3
In This Guide…
This manual covers the following:
•Agilent InfinityLab LC Series 1290 Infinity II Valve Drive (G1170A)
•Agilent InfinityLab Quick Change Valves
1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC
Series Valves
This chapter gives an introduction to the module, instrument overview and internal
connectors
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical and
performance specifications.
3 Installing the Module
This chapter gives information about the installation of the valve drive and the
valve heads.
4UsingtheModule
This chapter provides information on how to use the module.
5Troubleshootingand Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic features
and the different user interfaces.
6 Error Information
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.

1290 Infinity II Valve Drive and Valve Heads User Manual 4
7 Maintenance
This chapter gives you an overview and instructions about the possible
maintenance and repair procedures that can be performed by the user.
8 Parts and Materials
This chapter provides information on parts and materials.
9 Identifying Cables
This chapter provides information on cables used with the Agilent Infinity and
Infinity II Series modules.
10 Hardware Information
This chapter describes the module in more detail on hardware and electronics.
11 Appendix
This chapter provides additional information on safety, legal and web.

1290 Infinity II Valve Drive and Valve Heads User Manual 5
Contents
1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC
Series Valves 8
Overview of the Valve Drive 9
Overview of the Agilent InfinityLab Quick Change Valves 13
Typical Applications 15
Leak and Waste Handling 21
2 Site Requirements and Specifications 26
Site Requirements 27
Physical Specifications 30
Performance Specifications 31
3 Installing the Module 32
Unpacking the Module 33
Installing the Valve Drive 35
Installing the Valve Heads 47
4UsingtheModule54
Turn on/off 55
Status Indicators 57
Hints for Successful Use 58
Solvent Information 59
Software Configuration 66
Agilent Local Control Modules 70
5Troubleshootingand Diagnostics 71
Overview of the Module’s Indicators and Test Functions 72

1290 Infinity II Valve Drive and Valve Heads User Manual 6
6 Error Information 73
What Are Error Messages 74
General Error Messages 75
Module Specific Error Messages 81
7 Maintenance 84
Introduction to Maintenance 85
Cautions and Warnings 86
Overview of Maintenance 88
Cleaning the Module 89
Replacing Parts of the Valve Head 90
Replacing the Fuses of the Infinity II Valve Drive 92
Replacing Valve Heads 94
Replacing the Module Firmware 99
8 Parts and Materials 100
Valve Drive Parts 101
Valve Options Overview 103
Accessory Kit 105
9 Identifying Cables 106
Cable Overview 107
Analog Cables 109
Remote Cables 111
CAN/LAN Cables 114
RS-232 Cables 115
USB 116

1290 Infinity II Valve Drive and Valve Heads User Manual 7
10 Hardware Information 117
Firmware Description 118
Electrical Connections 121
Interfaces 124
Config Switch Settings of the Infinity II Valve Drive 132
11 Appendix 134
General Safety Information 135
Waste Electrical and Electronic Equipment (WEEE) Directive 141
Radio Interference 142
Sound Emission 143
Solvent Information 144
Agilent Technologies on Internet 145

1290 Infinity II Valve Drive and Valve Heads User Manual 8
1 Introduction to the Agilent 1290 Infinity II
Valve Drive and InfinityLab LC Series Valves
Overview of the Valve Drive 9
Overview of the Agilent InfinityLab Quick Change Valves 13
Typical Applications 15
Dual and Multiple Column Selection 15
Sample Enrichment and Sample Cleanup 17
Alternating Column Regeneration 19
Solvent Selection 20
Leak and Waste Handling 21
Leak Sensor 25
Waste Concept 25
This chapter gives an introduction to the module, instrument overview and
internal connectors

1290 Infinity II Valve Drive and Valve Heads User Manual 9
1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC Series
Valves
Overview of the Valve Drive
Overview of the Valve Drive
The Agilent 1290 Infinity II Valve Drive (G1170A) is a part of the InfinityLab LC
Series. This is a valve drive delivered without a specific valve head. To be used in
a HPLC system it is required to add a valve head to this device. It is compatible
with all currently available Agilent InfinityLab Quick Change Valves (see Table 1
on page 13)
It includes the following features:
•Built-in power supply
•Leak handling with a leak pane and a leak sensor underneath the valve head
•User exchangeable valve heads (Agilent InfinityLab Quick Change Valves)
•Valve type and pressure detected by RFID tag
•Flexible mounting bracket, for left- or right-side mounting on LC stacks or at
the Agilent Column Organizer (G1383A).

1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC Series
Valves
Overview of the Valve Drive
1290 Infinity II Valve Drive and Valve Heads User Manual 10
Figure 1 Mounting Examples for 1290 Infinity II Valve Drive

1290 Infinity II Valve Drive and Valve Heads User Manual 11
1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC Series
Valves
Overview of the Valve Drive
Figure 2 Overview of 1290 Infinity II Valve Drive
Clamps (attachable to both sides)
Valve Drive
Status indicator
Changeable valve head
Leak sensor
Attachable leak plane

1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC Series
Valves
Overview of the Valve Drive
1290 Infinity II Valve Drive and Valve Heads User Manual 12
Figure 3 Rear View of 1290 Infinity II Valve Drive
Fuses
External leak sensor
connector
Config switch
CAN ports
Power switch
Power connector

1290 Infinity II Valve Drive and Valve Heads User Manual 13
1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC Series
Valves
Overview of the Agilent InfinityLab Quick Change Valves
Overview of the Agilent InfinityLab Quick Change
Valves
The Agilent InfinityLab Quick Change Valves can be installed in the Multicolumn
Thermostat (G7116A/B), the 1290 Infinity Flexible Cube (G4227A) and in the
1290 Infinity II Valve Drive (G1170A).
For bio-inert modules use bio-inert parts only!
For 1290 Infinity II Bio LC modules, use bio / bio-compatible parts only.
Do not mix parts between 1260 Infinity II Bio-Inert LC modules and 1290 Infinity II Bio LC modules.
Table 1 Overview of the available Agilent Quick Change Valve Kits
Valve Kit PN Pressure
[bar]
Positions Ports Typical Application
G4231A 800 2 6 Any two-way switching, e.g.
between two detectors, between
waste and detector, between two
columns
G4231C 1300 2 6
G4232C 800 2 10 Anything a 2-position/6-port valve
can do plus alternating column
regeneration.
G4232D 1300 2 10
G4234A 800 6 14 6 column selection
G4234C 1300 6 14

1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC Series
Valves
Overview of the Agilent InfinityLab Quick Change Valves
1290 Infinity II Valve Drive and Valve Heads User Manual 14
G4235A 210 12 13 Solvent selection or fractionation
G4237A 800 4 10 4 column selection
G4239C 1300 8 18 8 column selection
G5631A 600 2 6 Bio-inert, same as G4231A
G5632A 600 2 10 Bio-inert, anything a
2-position/6-port valve can do plus
alternation column regeneration
G5639A 600 4 10 Bio-inert 4 column selection
G5641A 1300 2 10 Bio, anything a 2-position/6-port
valve can do plus alternation
column regeneration
Table 1 Overview of the available Agilent Quick Change Valve Kits
Valve Kit PN Pressure
[bar]
Positions Ports Typical Application

1290 Infinity II Valve Drive and Valve Heads User Manual 15
1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC Series
Valves
Typical Applications
Typical Applications
Agilent InfinityLab Quick Change Valves support a variety of valve applications.
They can be mounted to an external 1290 Infinity II Valve Drive (G1170A) or a
Multicolumn Thermostat (G7116A/B).
Examples of typical applications are:
•Dual and multiple column selection
•Sample enrichment and sample cleanup
•Alternating column regeneration
•Solvent selection
Dual and Multiple Column Selection
Dual Column Selection
Advantages:
•Increase productivity
•Higher instrument up-time
•Faster method scouting
Quickly change between two different stationary phases to check your
separation selectivity, or use two identical stationary phases to have the second
column immediately available after the first one loses efficiency, for example
with complex matrices.

1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC Series
Valves
Typical Applications
1290 Infinity II Valve Drive and Valve Heads User Manual 16
Multiple Column Selection
With the 6-column selection valve (G4234A/C) and the capillary kit for column
selection you can set up your system for use with up to 6 columns as displayed
in Figure 4 on page 16. Or you can use the system with 5 columns and one flow
path for flushing the system. This setup allows you to switch between columns
for faster method development. The multicolumn setup might also be used, if
several operators are sharing the system.
Figure 4 Multiple Column Selection (Example of Schematic Setup for 6-Column Selector)

1290 Infinity II Valve Drive and Valve Heads User Manual 17
1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC Series
Valves
Typical Applications
Sample Enrichment and Sample Cleanup
Sample Enrichment
Sample Cleanup
Position 1-6
Pump A / autosampler
Waste
Enrichment column
Pump B
Analytical
detector
column /
Position 1-2
Pump A / autosampler
Waste
Enrichment column
Pump B
Analytical
detector
column /
Pump A / autosampler
Detector
Analytical
column Waste
Pump B
Precolumn
Position 1-6
Position 1-2
Pump A / autosampler
Detector
Analytical
column Waste
Pump B
Precolumn

1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC Series
Valves
Typical Applications
1290 Infinity II Valve Drive and Valve Heads User Manual 18
Advantages:
•Easy automation of sample preparation
•Higher reproducibility
•Increased productivity and sensitivity
Sample cleanup is essential for samples with complex matrices, such as food
extracts and waste water. Before injection into a LC or LC/MS system, the
sample matrix must be separated from the analytes of interest. Otherwise,
contaminants can disrupt separation and detection or even damage the
analytical column.
Enrichment methods
Enrichment methods are the techniques of choice to obtain highest sensitivity
and to remove the sample matrix. The analytes are retained and concentrated
onto the precolumn, while the sample matrix is passed to waste. After the valve
switch, a second pump backflushes the analytes out of the precolumn onto the
separation column. This allows injection of large volumes onto the precolumn,
significantly expanding sensitivity in the range of ten to several thousands.
Sample cleanup
Cleanup methods handle analytes and matrices in the opposite way to
enrichment methods. Matrix components are retained on the precolumn while
the analytes pass through to the separation column. After the valve switches, an
extra pump backflushes the matrix components out of the precolumn to waste,
while the analytes are separated on the main column. Backflushing prepares the
precolumn for the next injection.

1290 Infinity II Valve Drive and Valve Heads User Manual 19
1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC Series
Valves
Typical Applications
Alternating Column Regeneration
Advantages:
•High sample throughput
•Increased productivity
•High efficiency
Gradient elution is frequently used for fast separation of complex samples in LC.
Since the gradient elution requires the column to regenerate before subsequent
runs, an automated column regeneration system saves valuable analysis time.
Agilent's InfinityLab Quick Change 2-position/10-port valve enables the
simultaneous analysis of one sample on one LC column while an extra
regeneration pump flushes and equilibrates a second, identical column. At the
end of the run, the valve switches to the second position and the next sample is
separated on the previously flushed and equilibrated column. Meanwhile, the
regeneration pump flushes and equilibrates the first column. Up to 50 % of
analysis time is often required to equilibrate columns. Using alternating column
regeneration saves time and provides higher sample throughput.
Column 2
Column 1
Eluent pump /
Autosampler
Regeneration
pump
Waste
Detector
Position 1-10
Column 2
Column 1
Eluent pump /
Autosampler
Regeneration
pump
Waste
Detector
Position 1-2

1 Introduction to the Agilent 1290 Infinity II Valve Drive and InfinityLab LC Series
Valves
Typical Applications
1290 Infinity II Valve Drive and Valve Heads User Manual 20
Solvent Selection
The 12-position/13-port solvent selection valve (G4235A) can be used for solvent
selection (flow rate < 10 mL/min) as illustrated in Figure 5 on page 20. It offers
automated access to 12 different eluents.
Figure 5 Solvent Selection (Schematic Setup)