Agilent Technologies 1200 Infinity Series User manual

Agilent Technologies
Agilent 1200 Infinity Series
High Dynamic Range Diode
Array Detector Solution
User Guide

HDR-DAD Solution
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Manual Part Number
G2199-90001 Rev. B
Edition
03/2017
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HDR-DAD Solution 3
In This Book
In This Book
This guide covers the Agilent 1200 Infinity Series High Dynamic Range Diode
Array Detector Solution (HDR-DAD)
Find detailed information on stack configurations and leak and waste
handling in the manuals of the modules, that are necessary to run this
solution.
1 What is the HDR-DAD Solution?
This chapter provides an overview of HDR-DAD and its intended use.
2 Specifications
This chapter provides information on performance specifications.
3 Installation of the HDR-DAD Solution
This chapter provides information on installation of the HDR-DAD Solution.
4 Using the HDR-DAD Solution
This chapter provides information on the main function of the user interface
for the HDR-DAD solution. For more details refer to the software manual or
the online help.
5 Frequently Asked Questions (FAQs)
This chapter answers frequently asked questions.
6 Application and Technical Notes for the HDR-DAD Solution
This chapter gives an overview on additional literature.

4HDR-DAD Solution
Contents
Contents
1 What is the HDR-DAD Solution? 5
30x wider linear UV-range – Quantification of widely different concentrations in a
single run 6
HDR-DAD Solution Data-Representation 8
2 Specifications 11
Specifications of the 1200 Infinity Series High Dynamic Range Diode Array
Detector Solution 12
3 Installation of the HDR-DAD Solution 15
Unpacking the Solution Kit 16
Flow connections and network setup 17
Enabling the HDR-DAD solution 21
Configuring the HDR-DAD Solution 23
Calculation of the Delay Volume 30
4 Using the HDR-DAD Solution 33
Status Information and Module Information Dialog of the HDR-DAD Cluster 34
Method settings in the HDR-DAD Solution 37
Status Display During a run with the HDR-DAD 40
Control User Interface of the HDR-DAD Solution 42
Method and Run Control User Interface in ChemStation 43
HDR-DAD Solution - Data Analysis 45
5 Frequently Asked Questions (FAQs) 47
Frequently Asked Questions 48
6 Application and Technical Notes for the HDR-DAD Solution 51
Application and Technical Notes 52

5
HDR-DAD Solution
Agilent Technologies
1
What is the HDR-DAD Solution?
30x wider linear UV-range – Quantification of widely different
concentrations in a single run 6
HDR-DAD Solution Data-Representation 8
This chapter provides an overview of HDR-DAD and its intended use.

6HDR-DAD Solution
1 What is the HDR-DAD Solution?
30x wider linear UV-range – Quantification of widely different concentrations in a single run
30x wider linear UV-range – Quantification of widely different
concentrations in a single run
The typical linear range of around 1·10
5
for UV-detection is not sufficient for
the analysis of samples or compound mixtures with widely different
concentration levels. Usually, these samples require additional sample
preparation steps (dilution/concentration), in combination with a second
injection and sometimes recalibration.
The HDR-DAD solution eliminates these sample preparation steps. It increases
the linear range by a factor of 30 and allows to acquire all sample information
in one single assay. It significantly reduces the turnaround time and increases
the productivity.
Figure 1 The HDR-DAD solution enables quantification of widely different
concentrations in one run

HDR-DAD Solution 7
What is the HDR-DAD Solution?
1
30x wider linear UV-range – Quantification of widely different concentrations in a single run
This is achieved by Agilent's Max-Light flow cells. Optofluidic waveguides in
these flow cells facilitate total internal reflection for superior light
transmission. This significantly reduces the detector noise regardless of the
length of the flow path.
The HDR-DAD solution combines two Agilent Diode Array Detectors
(possible combinations: G4212A/B x G4212A/B or G7117A/B x G7117A/B);
one is fitted with a 3.7 mm path length flow cell, while the other has a longer
60 mm path length flow cell. The signal from the longer path length cell
quantifies the low concentrations and the signal from the shorter path length
cell is used for the high concentrations. Special algorithms correct the
different retention times and compute these signals to one HDR signal.
Compared with an earlier generation Agilent diode array detector, the HDR
signal covers a 30 times higher linear dynamic range.
Achieve up to 30-fold greater UV-sensitivity
The Agilent HDR-DAD solution extends the upper linearity level nearly
three-fold – from 2 to typically 6 absorbance units – which allows you to inject
three-fold more sample and simultaneously benefit from 10-fold lower
detector noise. This solution gives you up to 30-fold greater sensitivity for
simultaneous analysis and quantification of main compounds and impurities.
You gain more confidence in automated peak integration and achieve greater
area precision for trace-level components.

8HDR-DAD Solution
1 What is the HDR-DAD Solution?
HDR-DAD Solution Data-Representation
HDR-DAD Solution Data-Representation
The HDR-DAD solution combines two Agilent Diode Array Detectors
(possible combinations: G4212A/B x G4212A/B or G7117A/B x G7117A/B).
The two detectors transfer their signals to the software. The algorithm of the
HDR-DAD solution combines and presents all signals to the user.
Figure 2 Signal representation in the HDR-DAD solution
It is important to know, which name corresponds to which signal, see
Table 1 on page 8 .
Signals
Table 1 Data representation in the HDR-DAD solution
Signal name Signal
DAD1 A HDR (computed by the weighting function algorithm of the HDR-DAD solution)
LNG1 A Long flow cell of DAD
1
SHT1 A Short flow cell of DAD
2

HDR-DAD Solution 9
What is the HDR-DAD Solution?
1
HDR-DAD Solution Data-Representation
NOTE
The HDR-DAD solution is available in ChemStation and EZChrom. Both software solutions
provide the same results.
Nevertheless, the two software solutions show different starting points of the HDR-DAD
signal:
•ChemStation:
The HDR signal is not starting from zero. The starting time of the HDR signal is
calculated with the configuration settings of the delay volume between detectors and
the linked pump.
•EZChrom:
The signal is displayed starting from zero and the delay is present at the end of the
chromatogram.
For details, see Ta b le 3 on page 46

10 HDR-DAD Solution
1 What is the HDR-DAD Solution?
HDR-DAD Solution Data-Representation