Agilent Technologies 1200 Infinity Series User manual

Agilent Technologies
Agilent 1200 Infinity Series
High Dynamic Range Diode
Array Detector Solution
User Guide

HDR-DAD Solution
Notices
© Agilent Technologies, Inc. 2014-2016,
2017
No part of this manual may be reproduced
in any form or by any means (including
electronic storage and retrieval or transla-
tion into a foreign language) without prior
agreement and written consent from Agi-
lent Technologies, Inc. as governed by
United States and international copyright
laws.
Manual Part Number
G2199-90001 Rev. B
Edition
03/2017
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Warranty
The material contained in this docu-
ment is provided “as is,” and is sub-
ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a par-
ticular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or perfor-
mance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the sep-
arate agreement shall control.
Technology Licenses
The hardware and/or software described in
this document are furnished under a
license and may be used or copied only in
accordance with the terms of such license.
Restricted Rights Legend
If software is for use in the performance of
a U.S. Government prime contract or sub-
contract, Software is delivered and licensed
as “Commercial computer software” as
defined in DFAR 252.227-7014 (June 1995),
or as a “commercial item” as defined in
FAR 2.101(a) or as “Restricted computer
software” as defined in FAR 52.227-19
(June 1987) or any equivalent agency regu-
lation or contract clause. Use, duplication
or disclosure of Software is subject to Agi-
lent Technologies’ standard commercial
license terms, and non-DOD Departments
and Agencies of the U.S. Government will
receive no greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will receive
no greater than Limited Rights as defined in
FAR 52.227-14 (June 1987) or DFAR
252.227-7015 (b)(2) (November 1995), as
applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully
understood and met.

HDR-DAD Solution 3
In This Book
In This Book
This guide covers the Agilent 1200 Infinity Series High Dynamic Range Diode
Array Detector Solution (HDR-DAD)
Find detailed information on stack configurations and leak and waste
handling in the manuals of the modules, that are necessary to run this
solution.
1 What is the HDR-DAD Solution?
This chapter provides an overview of HDR-DAD and its intended use.
2 Specifications
This chapter provides information on performance specifications.
3 Installation of the HDR-DAD Solution
This chapter provides information on installation of the HDR-DAD Solution.
4 Using the HDR-DAD Solution
This chapter provides information on the main function of the user interface
for the HDR-DAD solution. For more details refer to the software manual or
the online help.
5 Frequently Asked Questions (FAQs)
This chapter answers frequently asked questions.
6 Application and Technical Notes for the HDR-DAD Solution
This chapter gives an overview on additional literature.

4HDR-DAD Solution
Contents
Contents
1 What is the HDR-DAD Solution? 5
30x wider linear UV-range – Quantification of widely different concentrations in a
single run 6
HDR-DAD Solution Data-Representation 8
2 Specifications 11
Specifications of the 1200 Infinity Series High Dynamic Range Diode Array
Detector Solution 12
3 Installation of the HDR-DAD Solution 15
Unpacking the Solution Kit 16
Flow connections and network setup 17
Enabling the HDR-DAD solution 21
Configuring the HDR-DAD Solution 23
Calculation of the Delay Volume 30
4 Using the HDR-DAD Solution 33
Status Information and Module Information Dialog of the HDR-DAD Cluster 34
Method settings in the HDR-DAD Solution 37
Status Display During a run with the HDR-DAD 40
Control User Interface of the HDR-DAD Solution 42
Method and Run Control User Interface in ChemStation 43
HDR-DAD Solution - Data Analysis 45
5 Frequently Asked Questions (FAQs) 47
Frequently Asked Questions 48
6 Application and Technical Notes for the HDR-DAD Solution 51
Application and Technical Notes 52

5
HDR-DAD Solution
Agilent Technologies
1
What is the HDR-DAD Solution?
30x wider linear UV-range – Quantification of widely different
concentrations in a single run 6
HDR-DAD Solution Data-Representation 8
This chapter provides an overview of HDR-DAD and its intended use.

6HDR-DAD Solution
1 What is the HDR-DAD Solution?
30x wider linear UV-range – Quantification of widely different concentrations in a single run
30x wider linear UV-range – Quantification of widely different
concentrations in a single run
The typical linear range of around 1·10
5
for UV-detection is not sufficient for
the analysis of samples or compound mixtures with widely different
concentration levels. Usually, these samples require additional sample
preparation steps (dilution/concentration), in combination with a second
injection and sometimes recalibration.
The HDR-DAD solution eliminates these sample preparation steps. It increases
the linear range by a factor of 30 and allows to acquire all sample information
in one single assay. It significantly reduces the turnaround time and increases
the productivity.
Figure 1 The HDR-DAD solution enables quantification of widely different
concentrations in one run

HDR-DAD Solution 7
What is the HDR-DAD Solution?
1
30x wider linear UV-range – Quantification of widely different concentrations in a single run
This is achieved by Agilent's Max-Light flow cells. Optofluidic waveguides in
these flow cells facilitate total internal reflection for superior light
transmission. This significantly reduces the detector noise regardless of the
length of the flow path.
The HDR-DAD solution combines two Agilent Diode Array Detectors
(possible combinations: G4212A/B x G4212A/B or G7117A/B x G7117A/B);
one is fitted with a 3.7 mm path length flow cell, while the other has a longer
60 mm path length flow cell. The signal from the longer path length cell
quantifies the low concentrations and the signal from the shorter path length
cell is used for the high concentrations. Special algorithms correct the
different retention times and compute these signals to one HDR signal.
Compared with an earlier generation Agilent diode array detector, the HDR
signal covers a 30 times higher linear dynamic range.
Achieve up to 30-fold greater UV-sensitivity
The Agilent HDR-DAD solution extends the upper linearity level nearly
three-fold – from 2 to typically 6 absorbance units – which allows you to inject
three-fold more sample and simultaneously benefit from 10-fold lower
detector noise. This solution gives you up to 30-fold greater sensitivity for
simultaneous analysis and quantification of main compounds and impurities.
You gain more confidence in automated peak integration and achieve greater
area precision for trace-level components.

8HDR-DAD Solution
1 What is the HDR-DAD Solution?
HDR-DAD Solution Data-Representation
HDR-DAD Solution Data-Representation
The HDR-DAD solution combines two Agilent Diode Array Detectors
(possible combinations: G4212A/B x G4212A/B or G7117A/B x G7117A/B).
The two detectors transfer their signals to the software. The algorithm of the
HDR-DAD solution combines and presents all signals to the user.
Figure 2 Signal representation in the HDR-DAD solution
It is important to know, which name corresponds to which signal, see
Table 1 on page 8 .
Signals
Table 1 Data representation in the HDR-DAD solution
Signal name Signal
DAD1 A HDR (computed by the weighting function algorithm of the HDR-DAD solution)
LNG1 A Long flow cell of DAD
1
SHT1 A Short flow cell of DAD
2

HDR-DAD Solution 9
What is the HDR-DAD Solution?
1
HDR-DAD Solution Data-Representation
NOTE
The HDR-DAD solution is available in ChemStation and EZChrom. Both software solutions
provide the same results.
Nevertheless, the two software solutions show different starting points of the HDR-DAD
signal:
•ChemStation:
The HDR signal is not starting from zero. The starting time of the HDR signal is
calculated with the configuration settings of the delay volume between detectors and
the linked pump.
•EZChrom:
The signal is displayed starting from zero and the delay is present at the end of the
chromatogram.
For details, see Ta b le 3 on page 46

10 HDR-DAD Solution
1 What is the HDR-DAD Solution?
HDR-DAD Solution Data-Representation

11
HDR-DAD Solution
Agilent Technologies
2
Specifications
Specifications of the 1200 Infinity Series High Dynamic Range Diode Array
Detector Solution 12
This chapter provides information on performance specifications.

12 HDR-DAD Solution
2 Specifications
Specifications of the 1200 Infinity Series High Dynamic Range Diode Array Detector Solution
Specifications of the 1200 Infinity Series High Dynamic Range
Diode Array Detector Solution
Table 2 Performance Specifications of the HDR-DAD solution
Type Specification Comments
Detection type 1024-element photodiode array
Light source Deuterium lamp equipped with RFID tag that holds
lamp typical information.
Number of signals 8
HDR-DAD Maximum
sampling rate
80 Hz
Short term noise < ± 0.85·10
-6
AU/cm
Typically < ± 0.60·10
-6
AU/cm
at 230/4 nm, slit width 4 nm,
TC 2 s, ASTM
Drift < 0.5·10
-3
AU/h at 230 nm
Linearity > 5.4 AU (5 %)
Typically 6.7 AU (5 %)
at 265 nm
Wavelength range 190 – 640 nm
Wavelength accuracy ± 1 nm self-calibration with deuterium
lines
Slit width Programmable: 1, 2, 4, 8 nm
Fixed slit: 4 nm
(with G7117B, G4212A)
(with G7117A, G4212B)
Diode width ~ 0.5 nm
Wavelength bunching 2 – 400 nm programmable in steps of 1 nm
Flow cells Max-Light Cartridge Cell
( 60 mm, V(s) 4.0 µL) (G4212-60007)
HDR Max-Light Cartridge Cell
( 3.7 mm, V(s) 0.4 µL) (G4212-60032)
70 bar (1015 psi) Maximum
Operating Pressure (MOP)
1
150 bar (2175 psi) Maximum
Incidential Pressure (MIP)
2
cartridge type, equipped with RFID
tags that holds cell typical
information.
Spectral tools Data analysis software for spectra
evaluation, including spectral libraries
and peak purity functions

HDR-DAD Solution 13
Specifications
2
Specifications of the 1200 Infinity Series High Dynamic Range Diode Array Detector Solution
Analog outputs Recorder/integrator: 100 mV or 1 V,
output range 0.001 – 2 AU, one output
Communications G4212A/B:
LAN, controller-area network (CAN),
RS-232C, APG Remote: ready
start, stop and shut-down signals
G7117A/B:
LAN, controller-area network (CAN),
USB, ERI/APG Remote: ready
start, stop and shut-down signals
GLP features RFID for electronics records of flow
cell and UV lamp conditions (path
length, volume, product number, serial
number, test passed, usage)
Early maintenance feedback (EMF) for
continuous tracking of instrument
usage in terms of lamp burn time with
user settable limits and feedback
messages. Electronic records of
maintenance and errors. Verification
of wavelength accuracy with
deuterium lines.
Safety and
maintenance
Extensive diagnostics, error detection
and display through Agilent Instant
Pilot and Agilent Lab Advisor
software. Leak detection, safe leak
handling, leak output signal for
shutdown of pumping system. Low
voltages in major maintenance areas.
Others Electronic temperature control (ETC)
for the complete optical unit
1
Maximum Operating Pressure (MOP): The maximum pressure at which the system can operate
continuously under normal conditions.
2
Maximum Incidential Pressure (MIP): The maximum pressure which the system can experience
during a short time.
Table 2 Performance Specifications of the HDR-DAD solution
Type Specification Comments

14 HDR-DAD Solution
2 Specifications
Specifications of the 1200 Infinity Series High Dynamic Range Diode Array Detector Solution

15
HDR-DAD Solution
Agilent Technologies
3
Installation of the HDR-DAD Solution
Unpacking the Solution Kit 16
Flow connections and network setup 17
Enabling the HDR-DAD solution 21
Configuring the HDR-DAD Solution 23
Calculation of the Delay Volume 30
This chapter provides information on installation of the HDR-DAD Solution.

16 HDR-DAD Solution
3 Installation of the HDR-DAD Solution
Unpacking the Solution Kit
Unpacking the Solution Kit
Ensure all parts and materials have been delivered. The delivery checklist for
the 1200 Infinity HDR-DAD Solution Kit (G2199AA) is shown below.
#p/n Description
1 5067-4660 Capillary, SST 0.11 mm ID 220 mm long
1 5063-6524 Caffeine in Water, 10 mg/L, 50 mL
1 5022-2159 Restriction capillary, SST 0.12 mm ID, 2 m long
1 G1680-63721 Network LAN Switch
3 8121-0008 LAN Cable, shielded
1 N/A HDR-DAD USB dongle
Contains licence to enable HDR-DAD solution
1 Power Cord Part number as ordered (country specific)
1 G2199-90000 Printed manual
1 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive)
For the installation of the HDR-DAD USB dongle into a
G7117A/B

HDR-DAD Solution 17
Installation of the HDR-DAD Solution
3
Flow connections and network setup
Flow connections and network setup
For details on the installation of the entire stack, the detector and the flow
cell, see the manual Agilent 1200 Infinity Series Diode Array Detectors or
Agilent 1290 Infinity II Diode Array Detector.
Parts required # p/n Description
2 G7117A/B_HDR Agilent 1290 Infinity II Diode Array Detector
The HDR-DAD solution supports all combinations of the two
mandatory DADs (G7117A/G7117A, G7117A/G7117B or
G7117B/G7117B).
OR 2 G4212A/B_HDR Agilent 1290 Infinity Diode Array Detector
The HDR-DAD solution supports all combinations of the two
mandatory DADs (G4212A/G4212A, G4212A/G4212B or
G4212B/G4212B).
1 G4212-60007 Max-Light Cartridge Cell (60 mm, V(
σ ) 4.0 µL)
1 G4212-60032 HDR Max-Light Cartridge Cell (3.7 mm, V(
σ ) 0.4 µL)
1 G2199AA Agilent 1200 Infinity HDR-DAD Solution Kit
NOTE
The detector with the long path cell needs to be the first one and the detector with the
short cell needs to be the second one.
•DAD
1
: 60 mm Cell - for low concentrations (high sensitivity)
•DAD
2
: 3.7 mm Cell - for high concentrations (lower sensitivity)

18 HDR-DAD Solution
3 Installation of the HDR-DAD Solution
Flow connections and network setup
1Install the DAD
2
on top of DAD
1
Figure 3 Stack and capillary connection for HDR-DAD (1290 Infinity Stack as an
example)
2Connect the capillaries from the TCC to DAD
1
and the outlet of DAD
1
to
inlet the of DAD
2
using Capillary, SST 0.11 mm ID 220 mm long
(5067-4660).
Solvent cabinet
TCC
ALS
Pump
DAD 2
Capillary
DAD 1
Inlet DAD 2
Outlet DAD 1

HDR-DAD Solution 19
Installation of the HDR-DAD Solution
3
Flow connections and network setup
3Setup the network, for an example see “Local LAN connection to a PC (not
via corporate LAN)” on page 20.
NOTE
•Connect both detectors to the LAN and in addition connect all the devices via CAN bus.
•Configure both LAN connections, otherwise HDR-DAD will remain offline.
•Use the included network switch, in case the LC instrument is directly connected to a
PC (not via corporate LAN).

20 HDR-DAD Solution
3 Installation of the HDR-DAD Solution
Local LAN connection to a PC (not via corporate LAN)
Local LAN connection to a PC (not via corporate LAN)
LAN-cable
PC
LAN-cable
DAD SHT (short)
DAD LNG (long)
Network switch