Agilent Technologies 1260 Infinity II User manual

Agilent Technologies
Agilent InfinityLab LC Series
1260 Infinity II
Fraction Collector
User Manual

1260 Infinity II Fraction Collector User Manual
Notices
© Agilent Technologies, Inc. 2017
No part of this manual may be reproduced
in any form or by any means (including
electronic storage and retrieval or transla-
tion into a foreign language) without prior
agreement and written consent from Agi-
lent Technologies, Inc. as governed by
United States and international copyright
laws.
Manual Part Number
G1364-90030
Edition
09/2017
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Warranty
The material contained in this docu-
ment is provided “as is,” and is sub-
ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a par-
ticular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or perfor-
mance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the sep-
arate agreement shall control.
Technology Licenses
The hardware and/or software described in
this document are furnished under a
license and may be used or copied only in
accordance with the terms of such license.
Restricted Rights Legend
If software is for use in the performance of
a U.S. Government prime contract or sub-
contract, Software is delivered and licensed
as “Commercial computer software” as
defined in DFAR 252.227-7014 (June 1995),
or as a “commercial item” as defined in
FAR 2.101(a) or as “Restricted computer
software” as defined in FAR 52.227-19
(June 1987) or any equivalent agency regu-
lation or contract clause. Use, duplication
or disclosure of Software is subject to Agi-
lent Technologies’ standard commercial
license terms, and non-DOD Departments
and Agencies of the U.S. Government will
receive no greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will receive
no greater than Limited Rights as defined in
FAR 52.227-14 (June 1987) or DFAR
252.227-7015 (b)(2) (November 1995), as
applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully
understood and met.

1260 Infinity II Fraction Collector User Manual 3
In This Guide
In This Guide
This manual contains technical reference information about the Agilent
InfinityLab LC Series 1260 Infinity II Preparative Fraction Collector
(G1364E).
1Introduction
This chapter gives an introduction to the module and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Fraction Collector
This chapter provides information on unpacking, checking on completeness,
stack considerations and installation of the module.
4 Using the Module
This chapter explains the essential operational parameters of the module.
5 Preparing the Fraction Collector
This chapter explains the operational parameters of the module.
6 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 1260 Infinity II Fraction Collector User Manual
In This Guide
8 Maintenance
This chapter describes the maintenance of the module.
9 Parts for Maintenance and Repair
This chapter provides information on parts for maintenance and repair.
10 Identifying Cables
This chapter provides information on cables used with the module.
11 Hardware Information
This chapter describes the module in more detail on hardware and electronics.
12 Appendix
This chapter provides additional information on safety, legal, and web.

1260 Infinity II Fraction Collector User Manual 5
Contents
Contents
1Introduction 9
Product Description 10
Features 11
Overview of the Module 12
Fraction Collector Principle 13
Leak and Waste Handling 14
2 Site Requirements and Specifications 17
Site Requirements 18
Physical Specifications 21
Performance Specifications 22
3 Installing the Fraction Collector 25
Unpacking the module 26
Optimizing the Stack Configuration 27
Install the Module 28
Installing Capillaries 32
Flow Connections to the Fraction Collector 37
Fraction Collector Trays 39
Configure Well-plate Types 42
Transporting the Fraction Collector 46
4 Using the Module 47
Configuration and Operation of the Fraction Collector 48
Solvent Information 58
Turn on/off 60
Status Indicators 62
Agilent Local Control Modules 63

6 1260 Infinity II Fraction Collector User Manual
Contents
5 Preparing the Fraction Collector 65
Best Practices 66
Solvent Information 67
Capillary Color Coding Guide 73
Install Capillary Connections 74
Setting up the Fraction Collector with the Instrument Control Interface 77
Method Parameter Settings 82
6 Troubleshooting and Diagnostics 91
User Interfaces 92
Agilent Lab Advisor Software 93
7 Error Information 95
What Are Error Messages 97
General Error Messages 98
Module Error Messages 105
8 Maintenance 117
Introduction to Maintenance 118
Warnings and Cautions 119
Cleaning the Module 120
Overview of Maintenance 121
Replace the Inlet/Waste Tubings (Preparative) 122
Replace the Valve to Needle Tubing (Preparative) 127
Exchange the Preparative Needle Assembly 130
Exchange the Needle/Capillary Carrier Assembly (Preparative) 133
Exchange the Diverter Valve 136
Exchange the Internal Tray 139
Repair or Exchange a Funnel of the Internal Tray 141
Exchange the Leak Sensor 143
Replace the Module Firmware 146

1260 Infinity II Fraction Collector User Manual 7
Contents
9 Parts for Maintenance and Repair 147
Preparative Fraction Collector Parts 148
Diverter-Valve Assembly 152
Supported Trays for a Fraction Collector 153
List of Recommended Test Tubes 154
List of Recommended Vials and Caps 155
List of Recommended Plates and Closing Mats 160
Fraction Collector Accessory Kits 162
10 Identifying Cables 163
Cable Overview 164
Analog Cables 166
Remote Cables 168
CAN/LAN Cables 172
RS-232 Cables 173
USB 174
11 Hardware Information 175
Firmware Description 176
Electrical Connections 179
Interfaces 182
Setting the 6-bit Configuration Switch 190
Early Maintenance Feedback 194
Instrument Layout 195
12 Appendix 197
General Safety Information 198
Waste Electrical and Electronic Equipment Directive 204
Radio Interference 205
Sound Emission 206
Installation of Stainless Steel Cladded PEEK Capillaries 207
Agilent Technologies on Internet 213

8 1260 Infinity II Fraction Collector User Manual
Contents

9
1260 Infinity II Fraction Collector User Manual
Agilent Technologies
1
Introduction
Product Description 10
Product Description (G1364E) 10
Features 11
Features (G1364E) 11
Overview of the Module 12
Overview of the Preparative Fraction Collector 12
Fraction Collector Principle 13
Leak and Waste Handling 14
Leak and Waste 14
Waste Concept 15
Leak Sensor 15
This chapter gives an introduction to the module and an instrument overview.

10 1260 Infinity II Fraction Collector User Manual
1 Introduction
Product Description
Product Description
Product Description (G1364E)
The 1260 Infinity II Preparative Fraction Collector is optimized for
laboratories performing semipreparative-scale purification of milligram to low
gram quantities with typical flow rates from 5 to 100 mL/min. This fraction
collector fits in the modular design of the InfinityLab LC Series, occupying
minimal bench space. Lowest delay volumes minimize peak dispersion and
carryover for highest purity and recovery. A wide variety of collection tubes as
well as microtiter plates are available. For high-throughput applications, up to
four fraction collectors can be clustered in a single LC system.
Figure 1 Overview of the fraction collector
6WDWXVLQGLFDWRU
3RZHUVZLWFK