Agilent Technologies 1260 Infinity II User manual

Agilent Technologies
Agilent InfinityLab LC Series
1260 Infinity II
Fraction Collector
User Manual

1260 Infinity II Fraction Collector User Manual
Notices
© Agilent Technologies, Inc. 2017
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Manual Part Number
G1364-90030
Edition
09/2017
Printed in Germany
Agilent Technologies
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76337 Waldbronn
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understood and met.

1260 Infinity II Fraction Collector User Manual 3
In This Guide
In This Guide
This manual contains technical reference information about the Agilent
InfinityLab LC Series 1260 Infinity II Preparative Fraction Collector
(G1364E).
1Introduction
This chapter gives an introduction to the module and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Fraction Collector
This chapter provides information on unpacking, checking on completeness,
stack considerations and installation of the module.
4 Using the Module
This chapter explains the essential operational parameters of the module.
5 Preparing the Fraction Collector
This chapter explains the operational parameters of the module.
6 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 1260 Infinity II Fraction Collector User Manual
In This Guide
8 Maintenance
This chapter describes the maintenance of the module.
9 Parts for Maintenance and Repair
This chapter provides information on parts for maintenance and repair.
10 Identifying Cables
This chapter provides information on cables used with the module.
11 Hardware Information
This chapter describes the module in more detail on hardware and electronics.
12 Appendix
This chapter provides additional information on safety, legal, and web.

1260 Infinity II Fraction Collector User Manual 5
Contents
Contents
1Introduction 9
Product Description 10
Features 11
Overview of the Module 12
Fraction Collector Principle 13
Leak and Waste Handling 14
2 Site Requirements and Specifications 17
Site Requirements 18
Physical Specifications 21
Performance Specifications 22
3 Installing the Fraction Collector 25
Unpacking the module 26
Optimizing the Stack Configuration 27
Install the Module 28
Installing Capillaries 32
Flow Connections to the Fraction Collector 37
Fraction Collector Trays 39
Configure Well-plate Types 42
Transporting the Fraction Collector 46
4 Using the Module 47
Configuration and Operation of the Fraction Collector 48
Solvent Information 58
Turn on/off 60
Status Indicators 62
Agilent Local Control Modules 63

6 1260 Infinity II Fraction Collector User Manual
Contents
5 Preparing the Fraction Collector 65
Best Practices 66
Solvent Information 67
Capillary Color Coding Guide 73
Install Capillary Connections 74
Setting up the Fraction Collector with the Instrument Control Interface 77
Method Parameter Settings 82
6 Troubleshooting and Diagnostics 91
User Interfaces 92
Agilent Lab Advisor Software 93
7 Error Information 95
What Are Error Messages 97
General Error Messages 98
Module Error Messages 105
8 Maintenance 117
Introduction to Maintenance 118
Warnings and Cautions 119
Cleaning the Module 120
Overview of Maintenance 121
Replace the Inlet/Waste Tubings (Preparative) 122
Replace the Valve to Needle Tubing (Preparative) 127
Exchange the Preparative Needle Assembly 130
Exchange the Needle/Capillary Carrier Assembly (Preparative) 133
Exchange the Diverter Valve 136
Exchange the Internal Tray 139
Repair or Exchange a Funnel of the Internal Tray 141
Exchange the Leak Sensor 143
Replace the Module Firmware 146

1260 Infinity II Fraction Collector User Manual 7
Contents
9 Parts for Maintenance and Repair 147
Preparative Fraction Collector Parts 148
Diverter-Valve Assembly 152
Supported Trays for a Fraction Collector 153
List of Recommended Test Tubes 154
List of Recommended Vials and Caps 155
List of Recommended Plates and Closing Mats 160
Fraction Collector Accessory Kits 162
10 Identifying Cables 163
Cable Overview 164
Analog Cables 166
Remote Cables 168
CAN/LAN Cables 172
RS-232 Cables 173
USB 174
11 Hardware Information 175
Firmware Description 176
Electrical Connections 179
Interfaces 182
Setting the 6-bit Configuration Switch 190
Early Maintenance Feedback 194
Instrument Layout 195
12 Appendix 197
General Safety Information 198
Waste Electrical and Electronic Equipment Directive 204
Radio Interference 205
Sound Emission 206
Installation of Stainless Steel Cladded PEEK Capillaries 207
Agilent Technologies on Internet 213

8 1260 Infinity II Fraction Collector User Manual
Contents

9
1260 Infinity II Fraction Collector User Manual
Agilent Technologies
1
Introduction
Product Description 10
Product Description (G1364E) 10
Features 11
Features (G1364E) 11
Overview of the Module 12
Overview of the Preparative Fraction Collector 12
Fraction Collector Principle 13
Leak and Waste Handling 14
Leak and Waste 14
Waste Concept 15
Leak Sensor 15
This chapter gives an introduction to the module and an instrument overview.

10 1260 Infinity II Fraction Collector User Manual
1 Introduction
Product Description
Product Description
Product Description (G1364E)
The 1260 Infinity II Preparative Fraction Collector is optimized for
laboratories performing semipreparative-scale purification of milligram to low
gram quantities with typical flow rates from 5 to 100 mL/min. This fraction
collector fits in the modular design of the InfinityLab LC Series, occupying
minimal bench space. Lowest delay volumes minimize peak dispersion and
carryover for highest purity and recovery. A wide variety of collection tubes as
well as microtiter plates are available. For high-throughput applications, up to
four fraction collectors can be clustered in a single LC system.
Figure 1 Overview of the fraction collector
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1260 Infinity II Fraction Collector User Manual 11
Introduction
1
Features
Features
Features (G1364E)
Purification efficiency
•Lowest delay volumes for minimum peak dispersion and carryover
•Automated delay calibration facilitates highest fraction purity while
maintaining high-precision sample recovery
Instrument efficiency
•Per module collection of up to 216 fractions in glass tubes with 4 outer
diameters of tubes available, or in microtiter plates
•Multiple collection modes with fraction triggering based on time, peak, or
mass for exact collection of required fractions
Laboratory efficiency
•Expandable capacity up to 864 fractions within the same footprint
•Smooth upgrade paths allow you to increase capacity based on demand
•Forced fume extraction enables use of fraction collector outside a fume
cupboard

12 1260 Infinity II Fraction Collector User Manual
1 Introduction
Overview of the Module
Overview of the Module
Overview of the Preparative Fraction Collector
Figure 2 Overview of the Preparative Fraction Collector
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'LYHUWHUYDOYH
1HHGOHFDUULHUDVVHPEO\
3RZHUVZLWFK

1260 Infinity II Fraction Collector User Manual 13
Introduction
1
Fraction Collector Principle
Fraction Collector Principle
The movements of the Fraction Collector components during the sequence are
monitored continuously by the Fraction Collector processor. The processor
defines specific time windows and mechanical ranges for each movement. If a
specific step of the sequence is not completed successfully, an error message is
generated.
The standard fractioning sequence occurs in the following order:
1The Fraction Collector starts always from the home position.
2When the sample is injected, the fraction probe with diverter valve moves to
the required position.
3When the trigger is given by the detector, the diverter valve opens to collect
the fraction.
4When the trigger is given by the detector, the diverter valve closes and the
arm moves to the next fraction position or back to the home position if this
function is chosen in the CDS.
Fractioning Sequence
Before the start of the sequence, and during an analysis, the diverter valve is
in the fraction start position. In this position, the mobile phase flows through
the diverter valve towards waste.

14 1260 Infinity II Fraction Collector User Manual
1 Introduction
Leak and Waste Handling
Leak and Waste Handling
Leak and Waste
1

1260 Infinity II Fraction Collector User Manual 15
Introduction
1
Leak and Waste Handling
Waste Concept
1Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45
with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide
to use your own waste solution, make sure that the tubes don't immerse in
the liquid.
Leak Sensor
CAUTION
Solvent incompatibility
The solvent DMF (dimethyl formamide) leads to corrosion of the leak sensor. The
material of the leak sensor, PVDF (polyvinylidene fluoride), is incompatible with DMF.
➔Do not use DMF.

16 1260 Infinity II Fraction Collector User Manual
1 Introduction
Leak and Waste Handling

17
1260 Infinity II Fraction Collector User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 18
Physical Specifications 21
Performance Specifications 22
This chapter provides information on environmental requirements, physical
and performance specifications.

18 1260 Infinity II Fraction Collector User Manual
2 Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Considerations
The module power supply has wide ranging capability. It accepts any line
voltage in the range described in Table 1 on page 21. Consequently there is no
voltage selector in the rear of the module. There are also no externally
accessible fuses, because automatic electronic fuses are implemented in the
power supply.
WARNING
Hazard of electrical shock or damage of your instrumentation
can result, if the devices are connected to a line voltage higher than specified.
➔Connect your instrument to the specified line voltage only.
WARNING
The module is partially energized when switched off, as long as the power cord is
plugged in.
Repair work at the module can lead to personal injuries, e.g. electrical shock, when
the cover is opened and the module is connected to power.
➔Always unplug the power cable before opening the cover.
➔Do not connect the power cable to the instrument while the covers are removed.
WARNING
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument from the
power line at any time.
➔Make sure the power connector of the instrument can be easily reached and
unplugged.
➔Provide sufficient space behind the power socket of the instrument to unplug the
cable.

1260 Infinity II Fraction Collector User Manual 19
Site Requirements and Specifications
2
Site Requirements
Power Cords
Country-specific power cords are available for the module. The female end of
all power cords is identical. It plugs into the power-input socket at the rear.
The male end of each power cord is different and designed to match the wall
socket of a particular country or region.
Agilent makes sure that your instrument is shipped with the power cord that
is suitable for your particular country or region.
WARNING
Absence of ground connection
The absence of ground connection can lead to electric shock or short circuit.
➔Never operate your instrumentation from a power outlet that has no ground
connection.
WARNING
Unintended use of supplied power cords
Using power cords for unintended purposes can lead to personal injury or damage
of electronic equipment.
➔Never use a power cord other than the one that Agilent shipped with this
instrument.
➔Never use the power cords that Agilent Technologies supplies with this instrument
for any other equipment.
➔Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
WARNING
Power cords
Solvents may damage electrical cables.
➔Prevent electrical cables from getting in contact with solvents.
➔Exchange electrical cables after contact with solvents.

20 1260 Infinity II Fraction Collector User Manual
2 Site Requirements and Specifications
Site Requirements
Bench Space
The module dimensions and weight (see Table 1 on page 21) allow you to place
the module on almost any desk or laboratory bench. It needs an additional
2.5 cm (1.0 inches) of space on either side and approximately 8 cm
(3.1 inches) in the rear for air circulation and electric connections.
If the bench shall carry a complete HPLC system, make sure that the bench is
designed to bear the weight of all modules.
The module should be operated in a horizontal position.
Condensation
NOTE
Agilent recommends that you install the HPLC instrument in the InfinityLab Flex Bench
rack. This option helps to save bench space as all modules can be placed into one single
stack. It also allows to easily relocate the instrument to another Lab.
CAUTION
Condensation within the module
Condensation can damage the system electronics.
➔Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
➔If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.