Agilent Technologies 2200 TapeStation User manual

Agilent Technologies
Agilent 2200 TapeStation
User Manual

2200 TapeStation
Notices
© Agilent Technologies, Inc. 2011, 2012
No part of this manual may be reproduced
in any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G2966-90001
Edition
08/2012
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
This product may be used as a com-
ponent of an in vitro diagnostic sys-
tem if the system is registered with
the appropriate authorities and com-
plies with the relevant regulations.
Otherwise, it is intended only for gen-
eral laboratory use.
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully under-
stood and met.

2200 TapeStation 3
Contents
Contents
1 Introduction to the 2200 TapeStation System 5
Overview of the System 6
2 Specifications 11
Technical Specifications 12
3 Installing the System 15
Unpacking the System 16
Contents of the 2200 ScreenPlex TapeStation 18
Installing the System 20
4 Using the 2200 TapeStation System 23
Intended Use of the 2200 TapeStation System 24
Performance Limitations of Use 25
Components Required by the User 26
Operating Procedure 27
How to prepare your samples 39
DNA Sample Preparation 41
5 Maintenance 43
General Information 44
Changing the Needle 45
6 Troubleshooting 49
Agilent 2200 Controller Software 50
Agilent 2200 ScreenPlex Software Error Messages 52
ScreenTape 53
Agilent 2200 TapeStation 54
ScreenPlex Software 55
Troubleshooting 2200 TapeStation ScreenPlex Images 57
Troubleshooting the Test Kit Manufacturers TestKitSuite.xml File 60

42200 TapeStation
Contents
7 Appendix 65
Product Notices 66
Sound Emission 67
The Waste Electrical and Electronic Equipment Directive 68
Technical Service 69

5
2200 TapeStation
Agilent Technologies
1
Introduction to the 2200 TapeStation
System
Overview of the System 6
ScreenTape Architecture 7
Agilent 2200 TapeStation Components 8
This chapter gives an introduction to the system

62200 TapeStation
1Introduction to the 2200 TapeStation System
Overview of the System
Overview of the System
The Agilent 2200 TapeStation system is a revolutionary tape-based platform
for simpler, faster and more reliable electrophoresis. It is made up of three
elements: a consumable tape (ScreenTape), an instrument (the 2200
TapeStation) and an analysis software. The system is very straightforward to
use, simply place the sample tubes and ScreenTape in to the 2200 TapeStation
and let it load, separate, image, analyse and present the results.
This User Manual guides the operation of ScreenTape, the 2200 TapeStation
and software for the analysis. The contents of the ScreenTape System are
detailed below.
Place ScreenTape and some tips in the 2200 TapeStation.
Place your samples in the 2200 TapeStation and click Start on the
controller software.
View your analysed results in around 1 min per sample.

2200 TapeStation 7
Introduction to the 2200 TapeStation System 1
Overview of the System
Information pertaining to the 2200 TapeStation can be found in:
•2200 TapeStation Technical Specification
•2200 TapeStation Components
•Installing the 2200 TapeStation
Information pertaining to sample and ScreenTape requirements can be found
in:
•ScreenTape Architecture
•Operating Procedure
ScreenTape Architecture
Figure 1 ScreenTape DS12
Barcode: The unique barcode tracks lane usage within individual ScreenTapes and
allows traceability of results.
Buffer Chamber: The buffer chamber is located at the top of the channel and contains an
optimised buffer for the effective separation of amplified DNA fragments.
Electrodes: The enclosed electrodes apply a current across the ScreenTape and
eliminate the need for any additional equipment.
Gel: The acrylamide-based gel contained within ScreenTape has been
developed specifically to resolve amplified DNA.
ScreenTape Details: The information detailed is specific to that particular ScreenTape and lists
the ScreenTape type, expiry date and serial number.

82200 TapeStation
1Introduction to the 2200 TapeStation System
Overview of the System
Agilent 2200 TapeStation Components
Lid: The 2200 TapeStation lid must be closed each time the instrument
controller software is initialized, and whilst the instrument is in operation.
LED: The LED will illuminate once the instrument is on. When the LED is
flashing slowly, the instrument is in use and the lid should not be opened,
rapid flashing indicates that the TapeStation requires some attention.
Sample Block: There are 2 sample blocks provided that can either hold 0.2 mL sample
tube strips or a 96 well plate.
Tip Holder: The tip holder can accommodate up to 16 TapeStation loading tips at any
one time.
ScreenTape: The tape must be placed into the holder with the barcode towards the
front of the instrument, facing towards the right.
USB Socket: The USB connector is inserted into the USB socket to link the laptop to the
2200 TapeStation.
Power-cable socket: The power cable must be connected to the 2200 TapeStation and the
relevant mains electricity outlet.

2200 TapeStation 9
Introduction to the 2200 TapeStation System 1
Overview of the System
Figure 2 2200 TapeStation (front view)
Figure 3 2200 TapeStation (back view)
Lid
ScreenTape
Tip holder
LED
Sample block
USB socket
On/off switch
Power-cable socket

10 2200 TapeStation
1Introduction to the 2200 TapeStation System
Overview of the System

11
2200 TapeStation
Agilent Technologies
2
Specifications
Technical Specifications 12
This chapter provides information on the specifications of the 2200
Ta p e S t a ti o n .

12 2200 TapeStation
2Specifications
Technical Specifications
Technical Specifications
2200 TapeStation
Input voltage: 12 V DC
Power consumption: 40 W (VA)
Current: 3 A
Interface: USB cable (PC comms.)
Instrument Housing: UL94/VO rated flame retardant cast polyurethane
Dimensions: 400 x 310 x 310 mm
Weight: 12.5 kg
Power Supply
Input voltage: 100 – 240 V AC
Input frequency: 50 – 60 Hz
Phase: 1
Current: 0.45 – 1.1 A
Environmental condition
Optimal operating
temperature
23 °C (73.4 °F).
Ambient operating
temperature
10 – 37 °C (50.0 – 98.6 °F).

2200 TapeStation 13
Specifications 2
Technical Specifications
NOTE
If the instrument is out of the recommended temperature range for the ScreenTape inserted
the following error message will appear in the controller software:
•If the quoted current temperature is above the specified range, please move the
system out of direct sunlight and away from any windows. Check that any air
conditioning is functioning.
•If the quoted current temperature is below the specified range please allow the
instrument to equilibrate to the ambient temperature, and avoid using in a cooled
area.

14 2200 TapeStation
2Specifications
Technical Specifications

15
2200 TapeStation
Agilent Technologies
3
Installing the System
Unpacking the System 16
Contents of the 2200 ScreenPlex TapeStation 18
The TapeStation 18
ScreenTape Products 19
Installing the System 20
Software Installation 20
Agilent 2200 TapeStation Set Up 21
This chapter gives information about how to install the system.

16 2200 TapeStation
3Installing the System
Unpacking the System
Unpacking the System
Unpacking the Agilent 2200 TapeStation
Do not attempt to unpack the 2200 TapeStation instrument until you have read the accom-
panying Site and Safety Manual.
1Remove the TapeStation from the packaging and place on a clean, dry, flat
surface.
2Allow the TapeStation to acclimatise to the ambient temperature of the
operating environment.
CAUTION
Condensation within the instrument
Condensation will damage the system electronics.
➔If your instrument was shipped in cold weather, leave it in its box and allow it to
warm slowly to room temperature to avoid condensation.
CAUTION
"Defective on arrival" problems
If there are signs of damage, please do not attempt to install the instrument. Inspection
by Agilent is required to evaluate if the instrument is in good working condition.
➔Notify your local Agilent Representative and the Technical support channel.
➔An Agilent service representative will inspect the instrument at your site and
initiate appropriate actions.
WARNING
Personal injury
The TapeStation is heavy.
➔Enlist the aid of a co-worker to share the lifting load to avoid personal injury.

2200 TapeStation 17
Installing the System 3
Unpacking the System
3Remove the label covering the tape holder, as shown in the image below.
Figure 4 Remove before use
Delivery Checklist
Ensure all parts and materials have been delivered with your system. The
delivery checklist is shown below.
Please report any missing or damaged parts to your local Agilent Technologies
sales and service office.
NOTE
Please note that ScreenTape and Reagent kits are not provided with instrument delivery
and must be purchased separately.

18 2200 TapeStation
3Installing the System
Contents of the 2200 ScreenPlex TapeStation
Contents of the 2200 ScreenPlex TapeStation
The TapeStation
Table 1 Agilent 2200 TapeStation ScreenPlex System (G2966AA)
Product Volume Properties
Agilent 2200
TapeStation
1 x The instrument for loading, electrophoresing, imaging and analysing double
stranded DNA products
ScreenPlex Software
and User Information
1 x CD The software required to drive the TapeStation and visualise the ScreenTape
analysis. Also includes the user manual.
Laptop 1 x Laptop Instrument Control Laptop
USB Cables/Power
supply units
1 xUSBcable
2 x power cords
1 x USB cable to connect the laptop to the TapeStation
1 x Power supply unit for the laptop
1 x Power supply unit for the TapeStation
Sample Block 1 x 0.2 mL strip
and
1 x96 well
microtitre plate
A removable sample block for the correct loading of samples within the
Ta p e S t a ti o n
Tip Holder 2 x A removable cartridge for loading tips placed in the TapeStation
TapeStation -
compatible tips
1 x 384 tips Loading tips for use in the TapeStation
TapeStation -
compatible 0.2 mL
tube strips
120 x strips and
lids
Tube strips for placing samples mixed with loading buffer into the TapeStation
TapeStation -
compatible 96 -well
plates
pack of 10 96 -well plates for placing samples mixed with loading buffer into the
Ta p e S t a ti o n
96 well plate foil seal pack of 100 Foil covers for the 96 -well plates
Tip loader (optional) 1 x For placing loading tips into the tip holder

2200 TapeStation 19
Installing the System 3
Contents of the 2200 ScreenPlex TapeStation
ScreenTape Products
5067-5373 ScreenTape DS12 7 ScreenTape
5067-5374 ScreenTape DS12 Reagents
•DS12 Ladder
•DS12 Loading Buffer
1 vial
150 µL
800 µL

20 2200 TapeStation
3Installing the System
Installing the System
Installing the System
Software Installation
The software for your Agilent 2200 TapeStation system is preinstalled on the
system laptop.
NOTE
For updates, or if you have to change the laptop, you may download the latest version of the
software from the Test Kit Manufacturer's website.
For details on installation of the software refer to the readme.txt file on the installation CD
Agilent 2200 TapeStation ScreenPlex SOFTWARE AND USER INFORMATION.