Agilent Technologies 2200 TapeStation User manual

Agilent Technologies
Agilent 2200 TapeStation
User Manual

2200 TapeStation
Notices
© Agilent Technologies, Inc. 2011, 2012
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Manual Part Number
G2966-90001
Edition
08/2012
Printed in Germany
Agilent Technologies
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76337 Waldbronn
This product may be used as a com-
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Otherwise, it is intended only for gen-
eral laboratory use.
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2200 TapeStation 3
Contents
Contents
1 Introduction to the 2200 TapeStation System 5
Overview of the System 6
2 Specifications 11
Technical Specifications 12
3 Installing the System 15
Unpacking the System 16
Contents of the 2200 ScreenPlex TapeStation 18
Installing the System 20
4 Using the 2200 TapeStation System 23
Intended Use of the 2200 TapeStation System 24
Performance Limitations of Use 25
Components Required by the User 26
Operating Procedure 27
How to prepare your samples 39
DNA Sample Preparation 41
5 Maintenance 43
General Information 44
Changing the Needle 45
6 Troubleshooting 49
Agilent 2200 Controller Software 50
Agilent 2200 ScreenPlex Software Error Messages 52
ScreenTape 53
Agilent 2200 TapeStation 54
ScreenPlex Software 55
Troubleshooting 2200 TapeStation ScreenPlex Images 57
Troubleshooting the Test Kit Manufacturers TestKitSuite.xml File 60

42200 TapeStation
Contents
7 Appendix 65
Product Notices 66
Sound Emission 67
The Waste Electrical and Electronic Equipment Directive 68
Technical Service 69

5
2200 TapeStation
Agilent Technologies
1
Introduction to the 2200 TapeStation
System
Overview of the System 6
ScreenTape Architecture 7
Agilent 2200 TapeStation Components 8
This chapter gives an introduction to the system

62200 TapeStation
1Introduction to the 2200 TapeStation System
Overview of the System
Overview of the System
The Agilent 2200 TapeStation system is a revolutionary tape-based platform
for simpler, faster and more reliable electrophoresis. It is made up of three
elements: a consumable tape (ScreenTape), an instrument (the 2200
TapeStation) and an analysis software. The system is very straightforward to
use, simply place the sample tubes and ScreenTape in to the 2200 TapeStation
and let it load, separate, image, analyse and present the results.
This User Manual guides the operation of ScreenTape, the 2200 TapeStation
and software for the analysis. The contents of the ScreenTape System are
detailed below.
Place ScreenTape and some tips in the 2200 TapeStation.
Place your samples in the 2200 TapeStation and click Start on the
controller software.
View your analysed results in around 1 min per sample.

2200 TapeStation 7
Introduction to the 2200 TapeStation System 1
Overview of the System
Information pertaining to the 2200 TapeStation can be found in:
•2200 TapeStation Technical Specification
•2200 TapeStation Components
•Installing the 2200 TapeStation
Information pertaining to sample and ScreenTape requirements can be found
in:
•ScreenTape Architecture
•Operating Procedure
ScreenTape Architecture
Figure 1 ScreenTape DS12
Barcode: The unique barcode tracks lane usage within individual ScreenTapes and
allows traceability of results.
Buffer Chamber: The buffer chamber is located at the top of the channel and contains an
optimised buffer for the effective separation of amplified DNA fragments.
Electrodes: The enclosed electrodes apply a current across the ScreenTape and
eliminate the need for any additional equipment.
Gel: The acrylamide-based gel contained within ScreenTape has been
developed specifically to resolve amplified DNA.
ScreenTape Details: The information detailed is specific to that particular ScreenTape and lists
the ScreenTape type, expiry date and serial number.

82200 TapeStation
1Introduction to the 2200 TapeStation System
Overview of the System
Agilent 2200 TapeStation Components
Lid: The 2200 TapeStation lid must be closed each time the instrument
controller software is initialized, and whilst the instrument is in operation.
LED: The LED will illuminate once the instrument is on. When the LED is
flashing slowly, the instrument is in use and the lid should not be opened,
rapid flashing indicates that the TapeStation requires some attention.
Sample Block: There are 2 sample blocks provided that can either hold 0.2 mL sample
tube strips or a 96 well plate.
Tip Holder: The tip holder can accommodate up to 16 TapeStation loading tips at any
one time.
ScreenTape: The tape must be placed into the holder with the barcode towards the
front of the instrument, facing towards the right.
USB Socket: The USB connector is inserted into the USB socket to link the laptop to the
2200 TapeStation.
Power-cable socket: The power cable must be connected to the 2200 TapeStation and the
relevant mains electricity outlet.

2200 TapeStation 9
Introduction to the 2200 TapeStation System 1
Overview of the System
Figure 2 2200 TapeStation (front view)
Figure 3 2200 TapeStation (back view)
Lid
ScreenTape
Tip holder
LED
Sample block
USB socket
On/off switch
Power-cable socket

10 2200 TapeStation
1Introduction to the 2200 TapeStation System
Overview of the System