Agilent Technologies G1364B User manual

s1
Agilent 1100 Series
Analytical and
Preparative Scale
Fraction Collectors
User’s Guide

Agilent 1100 Series Fraction Collectors User’s Guide
Notices
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Manual Part Number
G1364-90001
Edition
07/03
Printed in Germany
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Agilent 1100 Series Fraction Collectors User’s Guide3
In This Guide…
This manual contains technical reference information about the Agilent 1100
Series fraction collectors analytical and preparative scale. The manual
describes the following:
1 Configuration and Operation of the Fraction Collector
This chapter describes the configuration and operation of the fraction
collector including guidelines to optimize the system and to avoid problems.
2 Troubleshooting and Test Functions
This chapter describes the modules built-in troubleshooting and test
functions.
3Repairing the Fraction Collector
This chapter contains instructions on simple repair and maintenance
procedures.
4Parts and Materials
This chapter contains lists for identification of common repair and
maintenance parts.
5Specifications
This chapter contains performance specifications of the fraction collectors.
ASafetyInformation
This appendix provides a safety summary.

4Agilent 1100 Series Fraction Collectors User’s Guide

Agilent 1100 Series Fraction Collectors User’s Guide 5
Contents
1 Configuration and Operation of the Fraction Collector 9
Configuration of the Fraction Collector 10
Configuration of the Fraction Collector in the ChemStation 10
Configuring multiple fraction collectors 14
Delay volumes and delay calibration 15
Handling of Delay times and volumes 15
Detector signal delay 16
Performing a delay calibration with an UV detector 18
Performing a delay calibration with an MSD 22
Making Your Own Calibration Method 26
Setting up a Fraction Collector Method 27
Fraction Preview 32
Setting up a Fraction Collection Sequence 34
Assignment of Start Location for fraction collection 34
Viewing your Results 36
Data Analysis 36
Report 36
Special Applications 38
Pooling 38
Sample Recovery 39
Semi-preparative operation 40
Using high test tubes in the analytical scale fraction collector 41
Optimizing fraction collection 42
Limitations and how to avoid problems 43

6 Agilent 1100 Series Fraction Collectors User’s Guide
Contents
Application Notes 44
2 Troubleshooting and Test Functions 45
Status Indicators 46
Error Messages 46
Maintenance Functions 46
Transport Unit Self Alignment 46
Status Indicators 47
Power Supply Indicator 47
Instrument Status Indicator 48
Maintenance Functions 49
Transport Unit Self Alignment 51
Step Commands 53
Troubleshooting 54
3 Repairing the Fraction Collector 55
Introduction into Repairing the Fraction Collector 56
Simple Repairs 56
Cleaning the Fraction Collector 56
Overview of Main Repair Procedures 57
Simple Repairs 58
Replacing the Inlet/Waste Tubings 59
Replacing the Valve to Needle Tubing 63
Exchanging the Preparative Needle Assembly 66
Exchanging the Analytical Needle Assembly 68
Exchanging the Diverter Valve 71
Exchanging the Internal Tray 74
Repairing or Exchanging a Funnel of the Internal Tray 76
4 Parts and Materials 79
Supported Trays for Fraction Collectors 80

Contents
Agilent 1100 Series Fraction Collectors User’s Guide 7
List of Recommended Test Tubes 82
List of Recommended Vials and Caps 83
List of Recommended Plates and Closing Mats 85
Transport Unit Assembly
(Preparative Scale) 87
Transport Unit Assembly
(Analytical Scale) 88
Needle Assemblies 89
Diverter-Valve Assembly 90
Tubing Kits 91
Internal Tray Assembly 92
Fraction Collector Accessory Kit 93
5 Specifications 95
Performance Specifications for the Fraction Collectors 96
ASafetyInformation101
Safety Information 102
General 102
Operation 103
Safety Symbols 104
Lithium Batteries Information 105
Radio Interference 106
Test and Measurement 106
Sound Emission 107
Manufacturer’s Declaration 107
Solvent Information 108
Solvents 108

8 Agilent 1100 Series Fraction Collectors User’s Guide
Contents
Agilent Technologies on Internet 109
Index 111

9
Agilent 1100 Series Fraction Collectors
User’s Guide
Agilent Technologies
1
Configuration and Operation of the
Fraction Collector
Configuration of the Fraction Collector 10
Delay volumes and delay calibration 15
Setting up a Fraction Collector Method 27
Setting up a Fraction Collection Sequence 34
Viewing your Results 36
Special Applications 38
Optimizing fraction collection 42
Limitations and how to avoid problems 43
Application Notes 44

10 Agilent 1100 Series Fraction Collectors User’s Guide
1 Configuration and Operation of the Fraction Collector
Configuration of the Fraction Collector
Configuration of the Fraction Collector
Configuration of the Fraction Collector in the ChemStation
In order to setup or change the configuration parameters of your fraction
collector select More Fraction Collector > Configuration from the Instrument menu
or right-click on the fraction collector icon in the graphical user interface. In
the resulting Fraction Collector Configuration dialog box (Figure 1 on page 11)
the configuration of the Trays, the Fraction Delay Volumes, the Collection
Order, the Needle Movement and the Well-Plates can be specified.
Trays
In the online mode of the Agilent ChemStation the configuration of the Trays
is recognized automatically. In the off-line mode an appropriate tray
configuration can be chosen from a wide variety of different trays and set-up
here. For a selection of supported trays see “Supported Trays for Fraction
Collectors” on page 80.
Tube volume [ml] Volume of the test tube should be specified 10% less than
the maximum fill volume to avoid overfill.
Tube he ight [ mm] The exact test tube height has to be specified. For
preparative scale fraction collector the minimum height is 48 mm and the
maximum height is 100 mm. For the analytical scale fraction collector with 50
mm needle (standard) the maximum height is 48 mm. By changing to the short
20 mm needle the maximum tube height can be in creased to 75 mm. For the
part numbers of the different needle assemblies see “Needle Assemblies” on
page 89.
If
CAUTION Before using the preparative scale fraction collector G1364B the delay calibration
adapter G1364-87301 has to be removed from the needle carrier assembly.
NOTE In the online ChemStation the installed tray is recognized and the default settings for tube
volume and tube height are loaded and displayed.