Agilent Technologies Agilent 1260 Infinity User manual

Agilent Technologies
Agilent 1260 Infinity
Binary LC
System User Guide

1260 Infinity Binary LC - System User Guide
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1260 Infinity Binary LC - System User Guide 3
In This Guide...
In This Guide...
This manual covers the Agilent 1260 Infinity Binary LC.
1 The Agilent 1260 Infinity Binary LC - Product Description
This chapter discusses the features of the 1260 Infinity Binary LC.
2 Introduction
This chapter gives an introduction to the Agilent 1260 Infinity Binary LC
and the underlying concepts.
3 Optimization of the Agilent 1260 Infinity Binary LC
This chapter considers how to apply the theory and use the features of
the LC system to develop optimized separations.
4 System Setup and Installation
This chapter includes information on software installation, installation of
the modules, and preparing the system for operation.
5QuickStartGuide
This chapter provides information on data acquisition and data analysis
with the 1260 Infinity Binary LC.
6 Appendix
This chapter provides additional information on safety, legal and web and
about setting up a method.

4 1260 Infinity Binary LC - System User Guide
Contents
Contents
1 The Agilent 1260 Infinity Binary LC - Product Description 7
Features of the Agilent 1260 Infinity Binary LC 8
System Components 10
Specifications 22
2 Introduction 27
Theory of Using Smaller Particles in Liquid Chromatography 28
Benefits of small particle size columns 34
Frictional Heating 37
3 Optimization of the Agilent 1260 Infinity Binary LC 39
How to Configure the Optimum Delay Volume 40
How to Achieve Higher Injection Volumes 47
How to Achieve Shorter Cycle Times 48
How to Achieve Lowest Carry-over 51
How to Achieve Higher Resolution 53
How to Achieve Higher Sensitivity 60
How to Prevent Column Blockages 65
4 System Setup and Installation 67
Installing Software 68
Installing the Modules 70
5QuickStartGuide 81
About the Quick Start Guide 82
Preparing the System 83
Data Acquisition in Method and Run Control View 88
Data Analysis 96

1260 Infinity Binary LC - System User Guide 5
Contents
6 Appendix 101
Safety Information 102
Solvent Information 105
Agilent Technologies on Internet 110
Setting Up a Method using Edit Entire Method 111

6 1260 Infinity Binary LC - System User Guide
Contents

7
1260 Infinity Binary LC - System User Guide
Agilent Technologies
1
The Agilent 1260 Infinity Binary LC -
Product Description
Features of the Agilent 1260 Infinity Binary LC 8
System Components 10
1260 Infinity Binary Pump (G1312B) 11
1260 Infinity High Performance Degasser (G4225A) 14
1260 Infinity High Performance Autosampler (G1367E) 15
1290 Infinity Thermostatted Column Compartment (G1316C) 17
1260 Infinity Diode Array Detector (G4212B) 19
1200 Infinity Series Quick Change Valves 21
Specifications 22
This chapter discusses the features of the 1260 Infinity Binary LC.

8 1260 Infinity Binary LC - System User Guide
1The Agilent 1260 Infinity Binary LC - Product Description
Features of the Agilent 1260 Infinity Binary LC
Features of the Agilent 1260 Infinity Binary LC
The design concept of the 1260 Infinity Binary LC is to provide a liquid
chromatograph offering ultra fast and high resolution separation capability
and yet which retains full functionality for standard HPLC applications.
Therefore, it provides full backwards compatibility for your established
HPLC and UHPLC methods. The use of sub- two micron (STM) particles
means that for high flowrates or long columns additional pressure is
required to drive the mobile phase through the column. The flowpath of
the 1260 Infinity Binary LC is optimized to produce minimal backpressure,
and ZORBAX RRHT columns have an engineered particle size distribution
that produces significantly less backpressure than other STM columns.
The design features and benefits of the Agilent 1260 Infinity Binary LC
are:
•The configurable delay volume down to 120 µL in the 1260 Infinity
Binary Pump combined with a flow range from 0.05 up to 5 mL/min at
pressures up to 600 bar provides universal applicability from narrow
bore (2.1 mm ID) to standard bore (4.6 mm ID) columns, matching the
needs for both LC and LC/MS.
•The standard delay volume configuration of the 1260 Infinity Binary
Pump allows you to run not only UHPLC but also conventional HPLC
methods without compromising performance or changing
chromatographic patterns.
•The next generation flow- through design of the 1260 Infinity High
Performance Autosampler achieves highest precision for a wide range of
injection volumes (from 0.1 up to 100 µL) without changing sample
loops. It is designed for high sample throughput, low carryover, and fast
injection cycles.
•High temperature, up to 100 °C on certain columns, allows more
selectivity flexibility and reduces solvent viscosity to allow even faster
separation.
•In the 1290 Infinity Thermostatted Column Compartment , different
heater (1.6 µL) and cooling elements (1.5 µL) for low extra- column
volume can be installed. The temperature is adjustable from 10 °C
below ambient up to 100 °C.

1260 Infinity Binary LC - System User Guide 9
The Agilent 1260 Infinity Binary LC - Product Description 1
Features of the Agilent 1260 Infinity Binary LC
•The new pull- out valve drive design and user- exchangeable
Quick- Change valves in the 1290 Infinity Thermostatted Column
Compartment boosts usability and paves the way for ultra
high- throughput, multi- method and automated method development
solutions.
•A low dispersion tubing kit and low volume flow cells minimize peak
dispersion for narrow bore columns.
•Baseline robustness and fast spectral acquisition at data rates up to
80 Hz through the new optical design of the 1260 Infinity Diode Array
Detector .
•Different UV detector flow cells for use with 2.1, 3.0 and 4.6 mm inner
diameter columns are available, including the revolutionary Agilent
Max- Light cartridge flow cell with 60 mm optical path length (typical
noise: <±0.6 µAU/cm) for ultra sensitivity in detection.
A stepwise upgrade from 1100 Series or 1200 Series to Agilent 1260
Infinity Binary LC is possible; for example a 1100 Series Detector or 1200
Series Column Compartment can be further used in combination with a
1260 Infinity Binary Pump.

10 1260 Infinity Binary LC - System User Guide
1The Agilent 1260 Infinity Binary LC - Product Description
System Components
System Components
Numerous system configurations of the 1260 Infinity Binary LC are
possible, tailored to the needs of your individual application requirements.
A few configurations are described in more detail in this manual (see
“System Setup and Installation” on page 67). The modules that are
described in the following sections are typical components of a 1260
Infinity Binary LC. In addition to these core components, individual
solutions are available for specific applications (some of which are
described in the Chapter "Optimization").