Agilent Technologies 1200 series User manual

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Agilent 1200 Series
Manual Injector
Reference Manual

2 1200 Series MI Reference Manual
Notices
© Agilent Technologies, Inc. 2006
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Manual Part Number
G1328-90010
Edition
02/06
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn, Germany
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met.

1200 Series MI Reference Manual 3
In This Guide…
1 Installing the Manual Injector
Site requirements and installation of the manual injector
2 Repairing the Manual Injector
Instructions on simple, routine repair procedures
3 Parts and Materials
Detailed illustrations and lists for identification of parts and
materials
4 Theory of Operation
Operation and mechanical hardware

4 1200 Series MI Reference Manual

1200 Series MI Reference Manual 5
Contents
1 Installing the Manual Injector
Unpacking the Manual Injector 8
Installing the Manual Injector 10
Flow Connections 14
Leak Drainage 16
Choice of Injection Seal 17
Needles 17
2 Repairing the Manual Injector
Overview of Repair Procedures 20
Flushing the Manual Injector 21
Cleaning the Manual Injector 21
Stator Face 22
Injection-Valve Seal 24
Position-Sensing Switch 26
3 Parts and Materials
Manual Injector 30
Injection-Valve Assembly 32
4 Theory of Operation
Description 36
Injecting Sample 37

6 1200 Series MI Reference Manual
A Safety Information
Solvent Information 40
Agilent Technologies on Internet 41
Index

7
Agilent 1200 Series Manual Injector
Reference Manual
Agilent Technologies
1
Installing the Manual Injector
Unpacking the Manual Injector 8
Installing the Manual Injector 10
Flow Connections 14
Leak Drainage 16
Choice of Injection Seal 17
Needles 17

8 1200 Series MI Reference Manual
1 Installing the Manual Injector
Unpacking the Manual Injector
Damaged Packaging
Upon receipt of your manual injector, inspect the shipping containers for any
signs of damage. If the containers or cushioning material are damaged, save
them until the contents have been checked for completeness and the manual
injector has been mechanically checked. If the shipping container or
cushioning material is damaged, notify the carrier and save the shipping
material for the carriers inspection.
Delivery Checklist
Ensure all parts and materials have been delivered with the manual injector.
The delivery checklist is shown in Table 1. To aid in parts identification,
please see “Parts and Materials” on page 29. Please report missing or damaged
parts to your local Agilent Technologies sales and service office.
CAUTION Ifthere are signs ofdamageto themanualinjector,please donotattempt toinstallthe
manual injector.
Table 1 Manual Injector Checklist
Description Quantity Part Number
Manual injection valve with start cable,including:
operating instructions, needle port cleaner, vent tubes
(×2) and fittings, 5/64 and 9/64-inch hex keys
1 5063-6502
Mounting pole 1 5001-3738
Connection capillary, 0.17 mm id, 500 mm 1 G1328-87600
Base plate 1 G1328-44111
Organizer plate 1 5042-8553
Catch tube cap 1 5042-8576

1200 Series MI Reference Manual 9
Installing the Manual Injector 1
Valve syringe, fixed needle 50 µl 1 5182-9619
Reference Manual 1 G1328-90010
Table 1 Manual Injector Checklist (continued)
Description Quantity Part Number

10 1200 Series MI Reference Manual
1 Installing the Manual Injector
Installing the Manual Injector
1Place the baseplate on the bench.
2Connect the two organizer plates to the base plate.
3Screw the mounting pole into one of the two holes in the organizer plate.
NOTE The manual injector can be installed at the left- or right-hand side of the instrument stack.
Figure 1 Connecting the Organizer Plates

1200 Series MI Reference Manual 11
Installing the Manual Injector 1
4Slide the manual injector onto the mounting pole (see Figure 2). Tighten the
mounting screw.
Figure 2 Installing the Mounting Pole and Manual Injector
Mounting screw

12 1200 Series MI Reference Manual
1 Installing the Manual Injector
5Install other system modules on top of the manual injector baseplate (see
Figure 3.
Figure 3 Installing the System

1200 Series MI Reference Manual 13
Installing the Manual Injector 1
6Connect the capillaries to the manual injector (see “Flow Connections” on
page 14).
Figure 4 Installing the Start Cable
HPI B HP- I BA dd ress
Position-sensor cable
Start cable
APG remote connector
See Figure 5

14 1200 Series MI Reference Manual
1 Installing the Manual Injector
Flow Connections
1Connect the pump outlet capillary to port 2.
2Connect the column-compartment inlet capillary to port 3.
3Connect the sample loop between ports 1 and 4.
4Connect one vent capillary (supplied with valve) to port 5 and one to port 6.
WARNING When opening capillary or tube fittings, solvents may leak out. Please observe
appropriate safety procedures (for example, goggles, safety gloves and protective
clothing) as described in the material handling and safety data sheetsupplied by the
solvent vendor, especially when toxic or hazardous solvents are used (see also
“Leak Drainage” on page 16).
CAUTION The outlets of the two vent capillaries (ports 5 and 6) and the needle port must be at
the same level to prevent siphoning (see Figure 6 on page 15).

1200 Series MI Reference Manual 15
Installing the Manual Injector 1
Figure 5 Flow Connections
Figure 6 Vent Capillaries
Sample loop
Waste
Vent tube
Sample loop
To column
From pump outlet
Vent capillaries
(ports 5 and 6)
Vent capillaries and
needle port at the
same level

16 1200 Series MI Reference Manual
1 Installing the Manual Injector
Leak Drainage
WARNING Check the manual injector fittings periodically for signs of leakage. In the eventof a
leak, solvent will drop into the leak channel in the baseplate, from where it is
channelled to the front and back of the baseplate.
Figure 7 Leak Drainage
Leak channel

1200 Series MI Reference Manual 17
Installing the Manual Injector 1
Choice of Injection Seal
The manual injector is supplied with a Vespel™injection seal as standard.
Vespel™is sensitive to alkaline attack, so avoid mobile phases with a pH of 10
or more. Use the Tefzel™ injection seal (see “Injection-Valve Assembly” on
page 32) for mobile phases with a pH above 10.
Needles
The manual injector is not supplied with syringes or needles.
Use needles with 0.028-inch outer diameter (22 gauge) × 2-inch long needle,
without electro-taper, and with 90ºpoint style (square tip).
CAUTION To prevent damage to the valve, always use the correct size needle.

18 1200 Series MI Reference Manual
1 Installing the Manual Injector

19
Agilent 1200 Series Manual Injector
Reference Manual
Agilent Technologies
2
Repairing the Manual Injector
Overview of Repair Procedures 20
Flushing the Manual Injector 21
Cleaning the Manual Injector 21
Stator Face 22
Injection-Valve Seal 24
Position-Sensing Switch 26

20 1200 Series MI Reference Manual
2 Repairing the Manual Injector
Overview of Repair Procedures
Table 2 Overview of Repair Procedures
Procedure Typical Frequency Time Required Notes
Flushing the injector After using aqueous buffers or salt
solutions 5 minutes
Exchanging the stator
face When visibly scratched, or when the valve performance shows indication
of leakage or wear
10 minutes See “Stator Face” on page 22
Exchanging the
injection-valve seal After approximately 10000 to 20000 injections, or when the valve
performance shows indication of
leakage or wear
10 minutes See “Injection-Valve Seal” on
page 24
Exchanging the
position-sensing
switch
When cable damaged or when no
startsignalis sent when switchingto
the inject position
10 minutes See “Position-Sensing Switch” on
page 26