Agilent Technologies 1260 Infinity User manual

Agilent Technologies
Agilent 1260 Infinity
Purification Solution
Operator's Quick
Reference Guide

Purification Solution - Operator's Guide
Notices
© Agilent Technologies, Inc. 2016
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Manual Part Number
M8368-90000 Rev. C
Edition
11/2016
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Purification Solution - Operator's Guide 3
In This Book
In This Book
This manual contains instructions for Operators on how to use the Automated
Purification software.
1 The Role of the Operator
This chapter describes the role of the user of the Automated Purification
Software in Easy Prep mode.
2 Executing a Purification Task
This chapter gives instructions for users with Easy Prep user rights on how to
execute a Purification Task.

4 Purification Solution - Operator's Guide
Contents
Contents
1 The Role of the Operator 5
2 Executing a Purification Task 7
Configuring a new task 8
Running a task 9
Processing partial data and cloning tasks 10
Reviewing the analytical results 12
Reviewing the purification results 13
Forcing the Purification Task to Close 14

5
Purification Solution - Operator's Guide
Agilent Technologies
1
The Role of the Operator
This chapter describes the role of the user of the Automated Purification
Software in Easy Prep mode.
Operators of the Automated Purification Software work in Easy Prep mode to
perform purification runs. If the samples have not been analyzed on a separate
analytical instrument, the purification runs include the analytical runs.
Typically, the Operators have been trained by a Method Developer on the work
environment and operating procedures.
The Operators:
•provide the samples for use in the purification run
•know the analytical and preparative instrument and operating system
settings that apply to their samples. These have been set up in the
purification software by a Method Developer.
•set up a purification run in the purification software by creating a
purification task based on a matching task template prepared by a Method
Developer. Operators can also create a new task as a clone of an existing
task, for example, to re-run a stopped task, to run samples of the original
task that have not yet been run, or to repeat a system suitability task.
•complete the missing information (analytical data source,
analytical-to-preparative vial mapping, and injection volume) to run the
task.
•run the purification task and review the results.
For Operators, access to the Automated Purification Software is limited to the
main purification workflow user interface (the purification task screen) and
its key settings (system definition, analytical and preparative run). While in
the purification task screen, ChemStation functionality is inaccessible to avoid
unintended interference. The ChemStation screen reports are thus not
suitable for Operators.
If Operators get stuck or need help, they should contact a Method Developer.

6 Purification Solution - Operator's Guide
1 The Role of the Operator
In This Book

7
Purification Solution - Operator's Guide
Agilent Technologies
2
Executing a Purification Task
Configuring a new task 8
Running a task 9
Processing partial data and cloning tasks 10
Reviewing the analytical results 12
Reviewing the purification results 13
Forcing the Purification Task to Close 14
This chapter gives instructions for users with Easy Prep user rights on how to
execute a Purification Task.
A purification task in the Automated Purification Software describes all
settings of a purification experiment, including its results, once the
experiment is complete.
Purification tasks are managed in the purification task screen, which is thus
the center for the execution of all purification work:
•configuration of the purification job (samples and operating setup)
•submission of the analytical and preparative runs (including progress
indication and stop)
•review and modification of the target identification from the analytical run
•review and export of the purification results (collected fractions)
1In the ChemStation Method and Run Control view, open the Purification menu
and select Tasks.
The purification task screen is shown, displaying in its upper region a list of
all tasks in the selected task folder. Details of the selected task (setup and
result info) are displayed in the lower region. By default, the configuration
tab with the Select System page of the selected task is displayed.

8 Purification Solution - Operator's Guide
2 Executing a Purification Task
Configuring a new task
Configuring a new task
1Click Add Task to select a task template or choose to clone the currently
selected task and create a new purification task.
2Verify that the Analytical System matches the system providing the analytical
run, and the Preparative System that corresponds to the current preparative
instrument configuration.
The current instrument configuration of the preparative system is
displayed in the table.
If one of the systems does not match, choose another template to create
your task. If there is no matching template, ask the Method Developer to
create one.
3Select the Analytical Run page and specify the source of the analytical run:
•To run an analytical scouting sequence on a combined analytical and
preparative system, select an existing ChemStation sequence or prepare
a sequence from scratch. Select the path and name of an existing
ChemStation sequence in the text input field or click Edit sequence to
start editing a sequence from scratch or modifying a selected sequence.
You can also import a sequence as a text (CSV or TXT) file.
•To use analytical results that have already been acquired on a
stand-alone system, specify the location of the source files (result set or
single sample results) in the text input field.
Select data files using the Process? checkbox in the sequence table.
4Select the Preparative Run page and specify a Preparative base method.
5Set the preparative vial locations and injection volumes. You may want to
apply a location mapping for vial locations. Specify the injection volume for
the first sample and use the fill-down option to apply the same volume for
other samples.

Purification Solution - Operator's Guide 9
Executing a Purification Task
2
Running a task
Running a task
1Click Run in the top toolbar of the Task dialog box to start the
purification run.
If the analytical sequence has not yet been run, it will be submitted to the
ChemStation run queue.
If the analytical run is already available, the process will start with the
evaluation of the analytical results.
The buttons in the top toolbar also allow you to stop a run in progress or
unschedule a scheduled run that has not yet started. If the run queue is
paused (for example, after a stop) you can resume it using the Resume
item in the Ta sk toolbar. Note that the / items can be helpful if the run
gets stuck or lost for any reason.
The run progress is displayed in the bottom line of the Ta s k screen. Run
state events and important processing events are displayed in the Logbook
tab.
If you stopped your run, or your run was aborted (by the instrument), call
your method developer. He can create a clone of your aborted task to enable
you to process the missed samples.

10 Purification Solution - Operator's Guide
2 Executing a Purification Task
Processing partial data and cloning tasks
Processing partial data and cloning tasks
You are dealing with partial data under the following conditions:
•You stopped your analytical or preparative run, or your run was stopped
due to an instrument error (such as solvent bottles running empty). In such
a case, some of the planned samples were executed but others were missed.
•You have a large set of analytical data from a separate analytical
instrument, but you want only a subset of it to be purified in one task. For
example, your preparative plate does not have the capacity to hold all
samples.
Task cloning (or copying) means creating a new task with the same settings
and analytical data as another task. The preparative results are not cloned.
1Select the task to be cloned in the task list of your purification task dialog
box.
2create a new task as a clone of the selected task by choosing Add clone of
currently selected task in the Add Purification Task dialog box.
If you want to repeat a (previous) system suitability test run, you can clone the
system suitability test task and let it run.
If your analytical run was stopped or aborted, the successfully processed
samples remain as analytical result data with the task. Purification proceeds
with those samples only.
1If you want to process the remaining/missed samples from such a task,
create a clone of the incomplete task.
2In the new cloned task, go to the Analytical Run page of the Task Configuration
and click Revert to sequence.
This restores your original sequence.
3Click Edit Sequence and remove the samples that already ran successfully.
4Click Accept changes to save your modified sequence with the task.
If your preparative run was stopped or aborted, the successfully processed
samples are saved with the task, which is now in a completed state, and no
further modifications can be made. You can proceed to reviewing your
purification results and export fractions as described in “Reviewing the
purification results” on page 13.