Agilent Technologies NanoDis User manual

Agilent NanoDis
Quick Start Guide
NanoDis Quick Start Guide
This guide covers the following modules: Agilent NanoDis System (G7937AA)
Additional Equipment Required (Not Supplied)
Agilent Technologies does not supply Cross Flow Filters. For further information and recommended suppliers,
contact the Dissolution team ([e-mail]).
Installation of Peristaltic Pump Tubing and Cassettes
To install the peristaltic pump tubing and cassettes, complete the following steps:
1Remove the cassettes by pressing the barbed part of the cassette and pushing it upwards.
2Using the peristaltic pump tubing, attach the tubing adapters and fasten them into the cassettes. Ensure that the
tubing clips are embedded correctly, see Figure 1 on page 1.
3Ensure that the pressure control lever is fully open. Re-insert the cassette into the Peristaltic Pump and secure
them by re-attaching the side support bar, see Figure 2 on page 1.
4Adjust the pressure control lever so that sufficient pressure is applied to the peristaltic pump tubing to allow free
flow of the media. A flow rate of 40 corresponds to an approx. flow rate of 6.0 mL/min
CAUTION
Damage of equipment by overpressure
A closed top horizontal filter connection during experimentation can cause potentially
damaging pressure build-up in the filter and the system.
Leave open the top horizontal connection of the filter during experimentation.
Figure 1 Peristaltic Pump Cassette Figure 2 Installing Cassettes

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NanoDis Quick Start Guide
NanoDis System Tubing Guide
The following Figure 3 on page 2 shows the components of the NanoDis System and provides information about the
required tubings.
Figure 3 Diagrammatic representation of the NanoDis System
Method Troubleshooting Guide
Controlling Peristaltic Pump Speed
The speed of the Peristaltic Pump is regulated by the manual controls on the front of the pump. It is suggested to run
a method with blank media and adjust the pump to achieve the correct speed. Start slowly, and increase the speed to
avoid overfilling, overpressure, or leakage.
Determination of Maximum Sample Volume
The maximum sample volume (VSampleMax) can be calculated as follows:
VSampleMax = VFilter — (VPrimeLoss — VPrimeTubing)
The Prime Tubing Volume (VPrimeTubing) is approximately 3.5 – 4.0 mL (determine based on supplied tubing.)
The Prime Loss Volume (VPrimeLoss) required is 3.5 mL minimum. It is suggested to add 0.5 mL to your Prime Loss
Volume, to ensure that the line is filled through the needle.
T1 708-DS – 850-DS Sample Cannula (6-Position: 1005–1920, 8-Position: 1005–1921)
T2 M1/4–28 to M1/4–28 Tubing Kit (1005–1729)
T3 Cannula Tubing Kit (5005–0066)
T4 Luer to M1/4–28 Tubing (1005–1953)
T5 708-DS – 850-DS Return Cannula (6-Position: 1005–1920, 8-Position: 1005–1921)
Table 1 Flow settings
Flow Setting Approximate Flow Rate
40 6.0 mL/min
60 8.5 mL/min
80 11.5 mL/min
NOTE
If the line is not filled sufficiently, the sample volume will be inaccurate as air will be delivered as part
of the desired sample volume.
T1 T2
T3 T4
T5
Agilent 708-DS
Dissolution Apparatus
Peristaltic
Pump
Hollow-Fiber
Filters
Agilent 850-DS
Dissolution Sampling Station
1 43
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NanoDis Quick Start Guide
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Example 1:
VPrimeLoss = 4.0 mL (programmed in system)
VFilter = 3.0 mL
VPrimeTubing = 3.5 mL
VSampleMax = 3.0 mL — (4.0 mL — 3.5 mL)
VSampleMax = 2.5 mL
Calculation of Filter Outer Volume
The outer volume of the filter (VFilterOut) can be calculated as follows:
VFilterOut = VFilterTotal — VFilterMembranes
The total volume of the filter (VFilterTotal) is:
VFilterTotal = × r1² × lFilterEffective
r1= internal radius of the filter
lFilterEffective = effective length of the filter
The volume of the filter membranes (VFilterMembranes) is:
VFilterMembranes = (× r2² × lFilterEffective) × nFilterMembranes
r2= radius of the filter membrane
lFilterEffective = effective length of the filter
nFilterMembranes = total number of membranes in the filter
Insufficient Sample
The following actions are suggested if there is insufficient amount of sample:
Filter Overfill or Insufficient Filling During Sampling
The following actions are suggested if the filter is overfilled or there is insufficient filling during sampling:
Table 2 Suggested actions in case of insufficient amount of sample
Observed Problem Suggested Actions
Insufficient sample
amount
Preconditioning
•Increase the Peristaltic Pump time in the Method Parameter tab.
Prime Cycle
•Reduce Prime Volume to transport more material from the filter into the needle.
Alternatively, set the Prime Loss Volume to 0 for the same effect.
Table 3 Suggested actions in case of filter overfill or insufficient filling during sampling
Observed Problem Suggested Actions
Insufficient filling Set Syringe Overlap to 0 and increase the Peristaltic Pump speed.
Filter overfill Increase Syringe Overlap time to maintain filter volume within a controllable range. Review the
Peristaltic Pump speed.

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Agilent Technologies Inc. 2022
Printed in Germany, Edition: 06/2022
*G7937-90000*
Part No: G7937-90000 Rev. A
Document No: D0016565 Rev. A
NanoDis Quick Start Guide
Strong Surfactant Media (e.g. > 5% SDS)
The following actions are suggested when using media with a high surfactant concentration (e.g. > 5% sodium
dodecyl sulfate).
Filter Care
Filter Conditioning
Condition the filters before using or storing them. For information about recommended volumes and media for filter
conditioning, refer to the documentation of the filter manufacturer (e.g. filter care guides).
Filter Cleaning
Create a cleaning method and use it after the analysis (post analysis). Use extended purge volumes and outer rinse
cycles, ensuring that sufficient volume is sampled through the needles. Replace the vessel media and rinse media for
this method.
Technical Support and Further Information
For technical support, contact dissolution.h[email protected].
Visit the Agilent Technologies website for useful information, support, and current developments about the products
and technology:
https://www.agilent.com/en/product/dissolution-testing/dissolution-apparatus/nanodis-system
Table 4 Suggested actions in case of media with high surfactant concentration
Observed Problem Suggested Actions
Foaming within lines or
filters
Reduce Peristaltic Pump speed, using the manual controls of the Peristaltic Pump.
Increase pump times proportionally, using the Method Development tab in the Dissolution
Workstation.
Foaming in syringes Reduce plunger speed, using the System Configuration tab in the Dissolution Workstation.
Increase aspiration dwell time in the 850-DS Autosampler, using the System Configuration tab
in the Dissolution Workstation.
It is possible to reduce the % surfactant required while increasing the Method rpm to overcome
foaming issues.
NOTE
Increased noise
The 850-DS Autosampler may generate increased noise when using lower plunger speeds. This is
normal behavior.