Agilent Technologies 1200 series User manual

Agilent Technologies
Agilent 1200 Series
Autosampler Thermostat
User Manual
1200 Series AT User Manual

1200 Series AT User Manual
Notices
© Agilent Technologies, Inc. 2007, 2008
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Manual Part Number
G1330-90011
Edition
11/08
Printed in Germany
Agilent Technologies
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76337 Waldbronn
Research Use Only
Not for use in Diagnostic Procedures.
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
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WARNING notice until the indi-
cated conditions are fully under-
stood and met.

1200 Series AT User Manual 3
Contents
Contents
1 Introduction 5
Introduction to the Thermostatted Autosampler 6
Autosampler Thermostat Operation 8
Electrical Connections 10
2 Site Requirements and Specifications 13
Site Requirements 14
Physical Specifications 17
Performance Specifications 18
3 Installing the G1330B Thermostat 19
Unpacking the Autosampler 20
Optimizing the Stack Configuration 22
Installing the G1330B Thermostat 24
Transporting the Thermostatted Autosampler 41
4 Optimizing Performance 43
Controller Requirements 44
5 Maintenance 45
Introduction into Repairing the Thermostat 46
6 Parts and Materials for Maintenance 55
Main Assemblies (External Parts) 56
Accessory Kit G1330-68705 57
Foam Parts 58
Plastic Parts 59
7 Cable Overview 61
Cable Overview 62
Analog Cables 64

41200 Series AT User Manual
Contents
Remote Cables 67
BCD Cables 72
Auxiliary Cable 74
CAN/LAN Cables 75
External Contact Cable 76
RS-232 Cables 77
8 Appendix 79
General Safety Information 80
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 83
Radio Interference 84
Sound Emission 85
Agilent Technologies on Internet 86

5
1200 Series AT User Manual
Agilent Technologies
1
Introduction
Introduction to the Thermostatted Autosampler 6
Autosampler Thermostat Operation 8
Electrical Connections 10

61200 Series AT User Manual
1Introduction
Introduction to the Thermostatted Autosampler
Introduction to the Thermostatted Autosampler
The Agilent 1200 Series autosampler is designed for use with other modules of
the Agilent 1200 Series LC system or with other LC systems if adequate
remote control inputs and outputs are available. The autosampler is controlled
from the Agilent 1200 Series control module or from the Agilent ChemStation
for LC systems.
The specially-designed thermostattable sample trays holds either 100 × 1.8 ml
vials or two wellplates and 10 × 1.8 ml vials.
The autosampler thermostat contains Peltier-controlled heat exchangers. A
fan draws air from the area above the sample vial tray of the autosampler and
is then blown through the fins of the cooling/heating module. There it is cooled
or heated according to the temperature setting. The thermostatted air enters
the autosampler through a recess underneath the specially-designed sample
tray. The air is then distributed evenly through the sample tray ensuring
effective temperature control, regardless of how many vials are in the tray.
In cooling mode condensation is generated on the cooled side of the Peltier
elements. This condensed water is safely guided into the leak system.

1200 Series AT User Manual 7
Introduction 1
Introduction to the Thermostatted Autosampler
Figure 1 Overview of the Autosampler Thermostat
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81200 Series AT User Manual
1Introduction
Autosampler Thermostat Operation
Autosampler Thermostat Operation
Figure 2 Autosampler Thermostat Principle
The thermostatted autosampler is equipped with a cooling/heating module
which uses Peltier elements for efficient air cooling. When turned on the front
side of the Peltier elements is heated/cooled according to the temperature
setting. A fan draws air from the sample tray area and blows it through the
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1200 Series AT User Manual 9
Introduction 1
Autosampler Thermostat Operation
channels of the heating/cooling module. The fan speed is determined
according to the environmental conditions (e.g., ambient temperature,
humidity). In the heating/cooling module the air reaches the temperature of
the Peltier elements and this thermostatted air is blown underneath the
special sample tray where it is evenly distributed and streams back into the
sample tray area. From there it is again drawn into the autosampler
thermostat. This “recycle” mode assures a very efficient cooling/heating of the
sample vials.
In cooling mode the opposite side of the Peltier element will become very hot
and to maintain the performance of the elements they have to be cooled down.
This is done with large heat exchangers in the back of the autosampler
thermostat. Four fans blow air from left to right through the instrument to
remove the heated air. The fan speed is controlled according to the
temperature of the Peltier elements.
During cooling condensation will appear in the heating/cooling module. The
condensed water will be guided out of the autosampler thermostat.

10 1200 Series AT User Manual
1Introduction
Electrical Connections
Electrical Connections
Figure 3 Electrical Connections
•The GPIB connector is used to connect the autosampler with a computer.
The address and control switch module next to the GPIB connector
determines the GPIB address of your autosampler. The switches are preset
to a default address (see Autosampler Reference Manual) and this is
recognized immediately after power on.
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1200 Series AT User Manual 11
Introduction 1
Electrical Connections
•The CAN bus is a serial bus with high-speed data transfer. The two
connectors for the CAN bus are used for internal Agilent 1200 Series
module data transfer and synchronization.
•The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
common shut down, prepare, and so on.
•The RS-232 connector may be used to control the autosampler from a
computer through an RS-232 connection, using appropriate software. This
connector needs to be activated by the configuration switch module next to
the GPIB connector. The software needs the appropriate drivers to support
this communication. See your software documentation for further
information.
•The Thermostat-Autosampler connection is used for control signal transfer
and synchronization of the two modules. The cable must be installed for
operation of the autosampler thermostat.
•The power input socket accepts a line voltage of 100–120 or
220–240 volts AC ± 10 % with a line frequency of 50 or 60 Hz. Maximum
power consumption of the autosampler module is 300 Watts (Volt-Amps).
Maximum power consumption of the autosampler thermostat module is
260 Watts (Volt-Amps). There are no voltage selectors on your autosampler
because the power supplies have automatic selection capability. The
autosampler module has no externally accessible fuses, because automatic
electronic fuses are implemented in the power supply. The power supply of
the autosampler thermostat has two externally accessible fuses. The
security lever at the power input socket prevents removal of the
autosampler cover when line power is still connected.
•The interface board slot is used for external contacts, BCD output and for
future use.

12 1200 Series AT User Manual
1Introduction
Electrical Connections

13
1200 Series AT User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 14
Power Consideration 14
Power Cords 15
Bench Space 16
Environment 16
Physical Specifications 17
Performance Specifications 18

14 1200 Series AT User Manual
2Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable site environment is important to ensure optimum performance of
the autosamplers.
Power Consideration
The autosamplers comprises two modules, the autosampler module (G1329A,
G1389A, G1367A, or G2260A) and the thermostat module (G1330B Therm).
Both modules have a separate power supply and a power plug for the line
connections. The two modules are connected by a control cable and both are
turned on by the autosampler module.
The autosampler power supplies have automatic voltage selectors (see Table 1
on page 17). Consequently there are no voltage selectors in the rear of the two
autosampler modules. The autosampler module has no externally accessible
fuses, because automatic electronic fuses are implemented in its power
supply. The autosampler thermostat power supply has two externally
accessible fuses.
WARNING
Incorrect line voltage at the instrument
Shock hazard or damage of your instrumentation can result, if the devices are
connected to a line voltage higher than specified.
➔Connect your instrument to the specified line voltage.
CAUTION
Unaccessable power plug.
In case of emergency it must be possible to disconnect the instrument from the power
line at any time.
➔Make sure the power connector of the instrument can be easily reached and
unplugged.
➔Provide sufficient space behind the power socket of the instrument to unplug the
cable.

1200 Series AT User Manual 15
Site Requirements and Specifications 2
Site Requirements
Power Cords
Different power cords are offered as options with the module. The female end
of all power cords is identical. It plugs into the power-input socket at the rear
of the module. The male end of each power cord is different and designed to
match the wall socket of a particular country or region.
WARNING
The absence of ground connection and the use of an unspecified power cord can
lead to electric shock or short circuit.
Electric Shock
➔Never operate your instrumentation from a power outlet that has no ground
connection.
➔Never use a power cord other than the Agilent Technologies power cord designed
for your region.
WARNING
Use of unsupplied cables
Using cables not supplied by Agilent Technologies can lead to damage of the
electronic components or personal injury.
➔Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.

16 1200 Series AT User Manual
2Site Requirements and Specifications
Site Requirements
Bench Space
The autosampler dimensions and weight (see Table 1 on page 17) allow the
instrument to be placed on almost any laboratory bench. The instrument
requires an additional 25 cm (10 inches) of space on either side for the
circulation of air, and approximately 8 cm (3.1 inches) at the rear for electrical
connections. Ensure the autosampler is installed in a level position.
If a complete Agilent 1200 Series system is to be installed on the bench, make
sure that the bench is designed to carry the weight of all the modules. For a
complete system including the thermostatted autosampler it is recommended
to position the modules in two stacks, see “Optimizing the Stack
Configuration” on page 22. Make sure that in this configuration there is 25 cm
(10 inches) space on either side of the thermostatted autosampler for the
circulation of air.
Environment
Your autosampler modules will work at ambient temperatures and relative
humidity as described in Table 1 on page 17.
CAUTION
Condensation within the module
Condensation will damage the system electronics.
➔Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
➔If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.

1200 Series AT User Manual 17
Site Requirements and Specifications 2
Physical Specifications
Physical Specifications
Table 1 Physical Specifications
Type Specification Comments
Weight 20.7 kg (46 lbs)
Dimensions
(width × depth × height)
140 × 345 × 435 mm (5.5 × 13.5 × 17
inches)
Line voltage 100 – 240 VAC, ± 10% Wide-ranging capability
Line frequency 50 or 60 Hz, ± 5%
Power consumption 260 VA / 210 W / 717 BTU Maximum
Ambient operating
temperature
4 – 55 °C (41 – 131 °F) See warning below
Ambient non-operating
temperature
-40–70 °C (-4–158 °F)
Humidity < 95%, at 25–40 °C (77–104 °F) Non-condensing
Operating Altitude Up to 2000 m (6500 ft)
Non-operating altitude Up to 4600 m (14950 ft) For storing the module
Safety standards: IEC, CSA,
UL
Installation Category II, Pollution
Degree 2
For indoor use only. Research
Use Only. Not for use in
Diagnostic Procedures.
WARNING
Hot rear panel
Using the autosampler at high environmental temperatures may cause the rear
panel to become hot.
➔Do not use the autosampler at environmental temperatures higher than 50 °C
(122 °F)

18 1200 Series AT User Manual
2Site Requirements and Specifications
Performance Specifications
Performance Specifications
Table 2 Performance Specifications Agilent 1200 Series thermostatted autosampler
Type Specification
Temperature range: setable from 4 °C to 40 °C in 1 ° increments
Temperature accuracy at ambient
temperatures < 25 °C and humidity < 50%
- 1°C to + 4 °C at a setpoint of 4 °C
Temperature accuracy at ambient
temperatures > 25 °C and/or humidity > 50%
- 1°C to + 5 °C at a setpoint of 4 °C

19
1200 Series AT User Manual
Agilent Technologies
3
Installing the G1330B Thermostat
Unpacking the Autosampler 20
Damaged Packaging 20
Delivery Checklist 20
Optimizing the Stack Configuration 22
Installing the G1330B Thermostat 24
Stage 1: Preparing the Autosampler Thermostat and Autosampler 26
Stage 2: Power Cable and Interface Cable Connection 30
Stage 3: Flow Connections 33
Stage 4: Installing the Sample Tray 34
Stage 5: Installing Tray Cover and Front Cover 37
Stage 6: Turning on the Thermostatted Autosampler 38
Stage 7: Update of Control Module Firmware 38
Stage 8: Update of the Agilent ChemStation Software 39
Transporting the Thermostatted Autosampler 41

20 1200 Series AT User Manual
3Installing the G1330B Thermostat
Unpacking the Autosampler
Unpacking the Autosampler
Damaged Packaging
The two modules of the autosampler are shipped in separate boxes. Upon
receipt of your autosampler, inspect the shipping containers for any signs of
damage. If the containers or cushioning material are damaged, save them until
the contents have been checked for completeness and the autosampler has
been mechanically and electrically checked. If the shipping container or
cushioning material is damaged, notify the carrier and save the shipping
material for the carriers inspection.
Delivery Checklist
Unpack the two boxes of the autosampler. Ensure all parts and materials have
been delivered with the autosampler and the autosampler thermostat. The
delivery checklist are shown in Table 3 on page 21. Please report missing or
damaged parts to your local Agilent Technologies sales and service office.
If the thermostatted autosampler was ordered as an upgrade (G1395A) to an
existing autosampler, the shipment will also contain the required software
upgrades for your Agilent ChemStation.
CAUTION
Mechanical damage of the autosampler
If the transport assembly is not parked, the autosampler could be damaged due to
excessive shock of the shipping container during transport.
➔Always park the transport assembly before shipment (see “Transporting the
Autosampler” in the corresponding manual).