Agilent Technologies Cary 8454 User manual


Agilent Cary 8454
UV-Visible Spectroscopy
System
Installation Guide

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
Notices
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2003-2008, 2011, 2013, 2014-2016
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Manual Part Number
G1115-90009
Edition
11/16
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Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 3
In This Guide…
This handbook describes what you have to do to install your UV-Visible
spectroscopy system. Most of the handbook deals with installing a complete
system, that is, the spectrophotometer, computer and printer. However, in the
second half of this handbook you will find additional information about
installing accessories, accessory boards, configuration, and software
installation.
1 Fast Installation
Use this chapter as an overview of the different actions you must perform to
install your UV-Visible spectroscopy system. If you are an experienced user of
analytical equipment from Agilent Technologies, this may be all you need. If
you need more information, this chapter guides you to other relevant areas in
this or another handbooks.
2 Installation and Start Up
Use this chapter if you need more detailed information about the installation
process.
3 Good Measurement Practices
Use this chapter if you require information about general operating conditions
to perform accurate and reliable measurements, for example, handling
samples, solvents and cells.
4 Installing Accessories
This chapter describes how to install the simple mechanical and optical
accessories that are available to expand the capabilities of your UV-Visible
spectrophotometer.
5 Installing the UV-Visible Software
This chapter describes what your computer requires, and what you must do to
install the software.

4 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
6 LAN Communication, Installation, Connection and Configuration
This chapter describes how to set up LAN communication between your
Agilent ChemStation and your UV-Visible spectrophotometer.
A Safety Information
This chapter contains relevant safety information.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 5
Contents
1 Fast Installation 9
Installing Preconfigured Agilent Cary 8454 UV-Visible Systems 10
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer
Systems 11
Installing Software Only 13
2 Installation and Start Up 15
Site Requirements for Your Spectrophotometer 17
Power considerations 17
Power cords 18
Space 18
Environment 19
Unpacking Your Spectrophotometer 20
Installing your UV-Visible Spectrophotometer 21
Instrument overview 21
Setting up the Spectrophotometer 24
Installing Your Computer 26
Setting up the computer 26
Starting the Spectrophotometer 27
Turning On 27
Instrument Warm-up 29
Starting the Agilent ChemStation Software 30
Operational Checkout 30

6 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
Contents
3 Good Measurement Practices 31
General Considerations 32
Spectrophotometer Design 32
Making Measurements 32
Sample Cell or Cuvette Material 33
Optical Specifications of Cells 34
Apertured Cells or Cuvettes 35
Flow Cells 36
Handling and Maintaining Cells or Cuvettes 37
Solvents 39
Sample Preparation 40
Photosensitive Samples 41
Stirring and Temperature Control 41
Checklist for Best Results 42
Inserting a Cell 44
4 Installing Accessories 45
Thermostattable Cell Holder 46
Installing a Thermostattable Cell Holder 47
Cell Stirring Module 48
Installing the Cell Stirring Module 49
Tubing Connections and Operation 50
Long Path Cell Holder 52
Installing the Long Path Cell Holder 53
Installing a Sample Cell 53
Optical Filter Wheel 55
Installing the Filter Wheel Assembly 57
Removing the Filter Wheel Assembly 58
5 Installing the UV-Visible Software 59
Agilent ChemStation Computer Details 60

Contents
Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 7
Minimum PC Requirements for Agilent ChemStation 60
Installing the Agilent ChemStation Using the DVD 63
Overview 63
Uninstalling Pre-existing Agilent UV-Visible ChemStation Software 63
Installation Procedure 64
Configuring Your System 72
6 LAN Communication, Installation, Connection and Configuration 75
Installing and Configuring TCP/IP on the PC 76
Installation of the TCP/IP protocol 76
Installing the LAN Interface in your UV-Visible Spectrophotometer 79
Connecting the Spectrophotometer to the PC 80
Single Instrument Installation 80
Multiple Instrument Installation 80
Setting the IP Address of the Spectrophotometer 81
Using the Agilent BootP Service 82
What is the Agilent BootP Service? 82
Addresses 83
Using the Agilent BootP Service Program 84
Configure the Agilent BootP Service Program 85
Configure the Agilent BootP Service 87
Configuring the G1369C LAN Card Using BootP 89
Using the Agilent ChemStation Configuration Editor to Configure the
Spectrophotometer 91
What is the Agilent ChemStation Configuration Editor? 91
Configuring an Agilent Cary 8454 UV-Visible Spectrophotometer 92
Troubleshooting LAN Communication 94
Power failure reported on the Agilent ChemStation 94
Frequent buffer overrun in Agilent Cary 8454 logbook 95

8 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
Contents
A Safety Information 97
Protective Earth 97
Disconnecting Device 97
UV Light Source 98
Equipment Class 98
Installation category 98
Pollution level 98
Index 99

9
Agilent Cary 8454 UV-Visible Spectroscopy System
Installation Guide
Agilent Technologies
1
Fast Installation
Installing Preconfigured Agilent Cary 8454 UV-Visible Systems 10
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible
Spectrophotometer Systems 11
Installing Software Only 13
Installation is straightforward, but if you have difficulties, or need more
information, references to detailed installation information are given.
Use this chapter if you are:
•installing a preconfigured UV-Visible spectroscopy system from Agilent
(including spectrophotometer, computer and software),
•installing a UV-Visible spectroscopy system not purchased as a
preconfigured system, or
•installing software only.

10 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
1 Fast Installation
Installing Preconfigured Agilent Cary 8454 UV-Visible Systems
Installing Preconfigured Agilent Cary 8454 UV-Visible Systems
If you have purchased one of these systems, the software is pre-installed and
preconfigured. You need only install and switch on the hardware.
✔Ensure you have space and an appropriate power supply.
For more information, see “Site Requirements for Your
Spectrophotometer” on page 17.
✔Unpack the system—check for damage and contents against packing list.
For more information, see “Unpacking Your Spectrophotometer” on
page 20.
✔Install your UV-Visible spectrophotometer.
•Connect line power to the rear.
•Connect the waste tubing underneath the sample pan.
For more information, see “Installing your UV-Visible
Spectrophotometer” on page 21.
✔Install your computer and printer.
•Connect the keyboard and mouse.
•Connect the monitor to line power and to the computer.
•Connect the printer to line power and to the computer.
•Connect the computer to the spectrophotometer with the LAN cable.
For more information, see “Installing Your Computer” on page 26 and
Chapter 6, “LAN Communication, Installation, Connection and
Configuration”.
✔Switch on the spectrophotometer (make sure the sample area is clear), wait
2 minutes for the lamps to ignite. When you are ready to operate your
spectrophotometer, switch on the computer and printer.
For more information, see “Starting the Spectrophotometer” on page 27.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 11
Fast Installation 1
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer Systems
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible
Spectrophotometer Systems
You must install and configure the software.
✔Ensure you have space and an appropriate power supply.
For more information, see “Site Requirements for Your
Spectrophotometer” on page 17.
✔Unpack the system - check for damage and contents against packing list.
For more information, see “Unpacking Your Spectrophotometer” on
page 20.
✔Install your UV-Visible spectrophotometer.
•Connect the line power to the rear.
•Connect the waste tubing underneath the sample pan.
For more information, see “Installing your UV-Visible
Spectrophotometer” on page 21.
✔If your computer and printer are not supplied by Agilent, ensure that the
requirements for proper operation of the software are met.
For more information, see “Minimum PC Requirements for
Agilent ChemStation” on page 60.
✔Connect the computer to the spectrophotometer with the LAN cable.
For more information, see “Installing Your Computer” on page 26 and “LAN
Communication, Installation, Connection and Configuration” on page 75.
✔Install your computer and printer.
•Connect the keyboard and mouse.
•Connect the monitor to the power supply and to the computer.
•Connect the printer to the power supply and to the computer.
•Connect the computer to the spectrophotometer with the LAN cable.
For more information, see “Installing Your Computer” on page 26.

12 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
1 Fast Installation
Installing Non-Preconfigured Agilent Cary 8454 UV-Visible Spectrophotometer Systems
✔Switch on the spectrophotometer (make sure sample area is clear) and wait
two minutes for the lamps to ignite. When you are ready to operate your
spectrophotometer, switch on the computer and printer.
For more information, see “Starting the Spectrophotometer” on page 27.
✔Install the Agilent ChemStation software.
For more information, see “Installing the Agilent ChemStation Using the
DVD” on page 63.
✔From the Windows Start menu, start the Agilent ChemStation software.
For more information, see “Starting the Agilent ChemStation Software” on
page 30.
✔Ensure that your software and spectrophotometer operate properly by
taking a blank measurement or performing the automatic self-test in the
diagnostic section of your software.
For more information, see “Operational Checkout” on page 30.
✔If you need information about solvents, sample preparation, blank
measurements, cuvette handling or how to insert a cuvette, see Chapter 3,
“Good Measurement Practices”.
✔Install any accessories.
For simple accessories see Chapter 4, “Installing Accessories”; for complex
accessories, see the instructions supplied with the accessory or the
Installation and Maintenance section of the Agilent ChemStation online
help.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 13
Fast Installation 1
Installing Software Only
Installing Software Only
✔Unpack the software—check for damage and check the contents against the
packing list.
✔Ensure that your computer and printer meet the requirements for proper
operation of the software.
For more information, see “Minimum PC Requirements for
Agilent ChemStation” on page 60.
✔Switch on the spectrophotometer (make sure the sample area is clear) and
wait two minutes for the lamps to ignite. When you are ready to operate
your spectrophotometer switch on the computer and printer.
✔From the Windows menu install the Agilent ChemStation software.
For more information, see “Installing the Agilent ChemStation Using the
DVD” on page 63.
✔From the Windows Start menu start the Agilent ChemStation software.
For more information, see “Starting the Agilent ChemStation Software” on
page 30.
✔Ensure that your software and spectrophotometer operate properly, by
taking a blank measurement or by performing the automatic self-test in the
diagnostic section of your software.
For more information, see “Operational Checkout” on page 30.

14 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
1 Fast Installation
Installing Software Only

15
Agilent Cary 8454 UV-Visible Spectroscopy System
Installation Guide
Agilent Technologies
2
Installation and Start Up
Site Requirements for Your Spectrophotometer 17
Unpacking Your Spectrophotometer 20
Installing your UV-Visible Spectrophotometer 21
Installing Your Computer 26
Starting the Spectrophotometer 27
Starting the Agilent ChemStation Software 30
Operational Checkout 30
The following topics are covered in this section:
•“Site Requirements for Your Spectrophotometer” on page 17 contains
information about electrical requirements such as power lines, power
cords, etc. It also gives recommendation for minimum available bench space
and environmental conditions in the laboratory.
•“Unpacking Your Spectrophotometer” on page 20 contains information
about what to do when the packing material is damaged or the shipment is
incomplete. It includes a list of the items which are supplied with the
instrument and computer.
•“Installing your UV-Visible Spectrophotometer” on page 21 gives an
overview of the instrument and explains buttons, indicators and the line
power switch. It explains where and how to connect line power,
communications and waste drain tubing.
•“Installing Your Computer” on page 26 describes how to install the
computer and peripherals, e.g. monitor, mouse, keyboard and printer.
•“Starting the Spectrophotometer” on page 27 describes the turn-on
procedure for the spectrophotometer and the warm up time required to
fulfill all specifications.

16 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
2 Installation and Start Up
•“Starting the Agilent ChemStation Software” on page 30 describes how you
start your Agilent ChemStation software.
•“Operational Checkout” on page 30—use this section to ensure that your
spectrophotometer, computer and the UV-Visible operating software is in
proper operating condition. There is an optional automated self-test which
can be used for a quick check of the spectrophotometer. This self-test can
only be executed after the instrument has warmed up for at least one hour.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 17
Installation and Start Up 2
Site Requirements for Your Spectrophotometer
Site Requirements for Your Spectrophotometer
Power considerations
Your spectrophotometer can operate on a line voltage of 100–240 V AC with a
line frequency of 50 or 60 Hz. Maximum power consumption is 220 VA.
The instrument power supply accepts any line voltage supplied in the above
mentioned range. Consequently there is no voltage selector in the rear of the
spectrophotometer. There are no externally accessible fuses, because
automatic electronic fuses are implemented in the power supply.
The computer and InkJet or LaserJet printers accept a nominal line voltage of
110 or 220 V AC with a line frequency of 50 or 60 Hz. For more information,
see the documentation that comes with your computer and/or printer.
WARNING
To disconnect the instrument from line power, disconnect the power cord from the
supply. The power supply still uses some power, even if the power switch on the
front panel is turned off.
WARNING
Connecting your instrument to a line voltage that is higher than specified causes a
shock hazard, and damage may occur to your instrument.

18 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
2 Installation and Start Up
Site Requirements for Your Spectrophotometer
Power cords
Different power cords are offered as options with the spectrophotometer. The
female end of each of the power cords is identical. This connects to the
power-input socket at the rear of the spectrophotometer. The male end of each
of the power cords is different and designed to match the wall socket of a
particular country or region.
Do not position the equipment so that it is difficult to operate the
disconnecting device.
Space
The spectrophotometer is 34.4 cm (13.5 inches) wide, 56.0 cm (22.0 inches)
deep and 18.5 cm (7.3 inches) high (see Figure 1 on page 22). It weighs 14 kg
(30.86 lbs) and will fit on almost any desk or laboratory bench. It needs an
additional 2.5 cm (1.0 inches) of space on either side to allow for the
circulation of air for cooling, and approximately 8 cm (3.1 inches) in the rear
for electrical connections.
The computer, including the keyboard, is approximately 50 cm (19.7 inches)
wide and 60 cm (23.6 inches) deep.
Additional space is required for accessories like pumps and autosamplers.
NOTE
Do not replace the mains power cord with one that is inadequately rated. When replacing
the mains power cord, use only a mains power cord with a rating equivalent to the one
supplied with the instrument. See the instrument installation guide for detailed mains
power cord rating information.
WARNING
Always operate your instrument from a power outlet which has a ground
connection. Always use the power cord designed for your region.

Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide 19
Installation and Start Up 2
Site Requirements for Your Spectrophotometer
Environment
Table 1 Suitable environmental conditions for the 8454 UV-Vis
Avoid air circulation of varying temperatures (like from refrigerators or air
conditioners) and exposure to direct sun light to ensure the instrument
operates within performance specifications.
Condition Altitude Temperature Humidity (%RH)
non-condensing
Non-operating
(Storage or shipping)
Up to 4,600 m
(14,950 ft.)
- 40 and 70 °C
(-4 to 158 °F)
90%
Operating within
specifications
Up to 2,000 m
(6,500 ft.)
5-40 °C
(41-104 °F)
<95%
NOTE
For optimum analytical performance, it is recommended that the ambient temperature of
the laboratory be between 20-25 °C and be held +/- 2 °C throughout the entire working day.
WARNING
If you use the instrument at environmental temperatures higher than 50°C (122°F),
the rear of the instrument may get hot.
CAUTION
Do not store, ship or use your instrument under conditions where temperature
fluctuations could cause condensation within the instrument. Condensation will
damage the electronics. If your instrument was shipped in cold weather, leave it in its
box and allow it to warm up slowly to room temperature to avoid condensation.

20 Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
2 Installation and Start Up
Unpacking Your Spectrophotometer
Unpacking Your Spectrophotometer
Upon receipt of your spectrophotometer, computer and printer, inspect the
shipping containers for any signs of damage. If the containers or cushioning
material are damaged, save them until the contents have been checked for
completeness and the spectrophotometer, computer or printer have been
mechanically and electrically checked. If the shipping container or cushioning
material is damaged, notify the carrier as well as Agilent Technologies. Save
the shipping material for the carrier’s inspection.
Check the contents of the shipping container against the list below. If any
items are missing or obviously damaged, call Agilent Technologies.
✔Unpack the spectrophotometer. You should have the following components:
•Power cord to connect the spectrophotometer to line power.
•LAN Interface, if you connect the spectrophotometer through a LAN
•Waste tubing to connect the sample pan of your spectrophotometer to a
waste container.
•Checkout sample (caffeine solution).
✔Unpack the computer and its accessories. You should have the following
components:
•Computer and power cable
•Keyboard
•Mouse
•Display unit, connecting cable and power cable
•Printer, connecting cable and power cable
•Printer accessories, for example, ink cartridge in case of a DeskJet
printer
WARNING
To avoid injury to personnel or damage to equipment, always use a two or more
people or suitable lifting device when moving the instrument.