Agilent Technologies 7200 User manual

Agilent Technologies
Agilent
7200 Accurate-Mass
Quadrupole
Time-of-Flight GC/MS
System
Operation Manual

Operation Manual
Notices
© Agilent Technologies, Inc. 2012
No part of this manual may be reproduced in
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Manual Part Number
G3850-90005
Edition
First Edition, February 2012
Printed in USA
Agilent Technologies, Inc.
5301 Stevens Creek Blvd.
Santa Clara, CA 95051
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A WARNING notice denotes a
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met.

Operation Manual 3
About This Manual
This manual contains information for operating and
maintaining the Agilent 7200 Accurate-Mass Quadrupole
Time-of-Flight GC/MS system.
1 “Introduction”
Chapter 1 describes general information about the 7200 Q-TOF
GC/MS, including a hardware description, and general safety
warnings.
2 “Installing GC Columns”
Chapter 2 shows you how to prepare a capillary column for use
with the MS, install it in the GC oven, and connect it to the MS
using the GC/MS interface.
3 “Operating in Electron Impact (EI) Mode”
Chapter 3 describes routine operations in EI mode such as
setting temperatures, monitoring pressures, tuning, venting,
and pumpdown.
4 “Operating in Chemical Ionization (CI) Mode”
Chapter 4 describes additional tasks necessary to operate in CI
mode.
5 “Using the Removable Ion Source”
Chapter 5 shows you how to change the removable ion source.
6 “General Maintenance”
Chapter 6 describes general maintenance procedures for the
7200 Q-TOF GC/MS.

4 Operation Manual
Hardware User Information
Now your Agilent instrument documentation is in one place, at
your fingertips.
The hardware user information DVDs that ship with your
instrument provide an extensive collection of online help,
videos, and books for the Agilent 7890A GC, 7200 Q-TOF GC/MS,
7693 ALS, and the 7683B ALS. Included are localized versions of
the information you need most, such as:
• Getting Familiar documentation
• Safety and Regulatory guides
• Site Preparation checklists
• Installation information
• Operating guides
• Maintenance information
• Troubleshooting details

Operation Manual 5
Contents
1 Introduction
Abbreviations Used 10
The 7200 Accurate-Mass Quadrupole Time-of-Flight GC/MS
System 12
Hardware Description 15
Important Safety Warnings 16
Safety and Regulatory Certifications 20
Intended Use 23
Cleaning/Recycling the Product 23
Liquid Spills 23
Moving or Storing the MS 23
2 Installing GC Columns
Columns 26
To Prepare a Capillary Column for Installation 29
To Install a Capillary Column in a Split/Splitless Inlet 31
To Condition a Capillary Column 33
To Install a Capillary Column in the GC/MS Interface 35
To Prepare the Column Ends for a CFT Fitting 38
3 Operating in Electron Impact (EI) Mode
Operating the MS from the Data System 44
The GC/MS Interface 45
Before You Turn On the MS 47
Pumping Down 48

6 Operation Manual
Controlling Temperatures 48
Controlling Column Flow 48
Controlling Collision Cell Flow 49
Venting the MS 50
Typical Vacuum Pressures in EI Mode 51
To Set Monitors for Temperature and Vacuum Status 52
To Set the MS Analyzer Temperature 55
To Set the GC/MS Interface Temperature from the MassHunter
Workstation 57
To Calibrate the Column 58
To Configure the Collision Cell Gas 60
To Set the Collision Cell Gas Flow Rate 61
To Autotune the MS for EI Mode 62
To Remove the Upper RIS Cover 64
To Open the Analyzer Cover for Access to the Analyzer
Sideplate 65
To Pump Down the MS 66
To Vent the MS 69
4 Operating in Chemical Ionization (CI) Mode
Setting Up Your MS to Operate in CI Mode 72
The CI GC/MS Interface 73
Operating the CI MS 75
High Vacuum Pressure in CI Mode 76
Other Reagent Gases 78
CI Autotune 80

Operation Manual 7
The Flow Control Module 82
To Switch from the EI Mode to CI Mode 83
To Operate the Reagent Gas Flow Control Module 85
To Set a Reagent Gas Flow 87
To Perform a CI Autotune 88
5 Using the Removable Ion Source
The Removable Ion Source 92
To Change the Ion Source 94
To Install the RIS Probe Extraction Tool 97
To Remove the Source from the Analyzer Chamber 99
To Change the Ion Source on the RIS Probe Extraction Tool 101
To Install the Ion Source in the Analyzer Chamber 103
To Remove the RIS Probe Extraction Tool from the
Instrument 104
6 General Maintenance
Before Starting 106
Maintaining the Vacuum System 111
Maintaining the Analyzer 112
To Disassemble the EI Ion Source 114
To Assemble the EI Ion Source 116
To Disassemble the CI Ion Source 118
To Assemble the CI Ion Source 120
To Clean the Ion Source 122
To Remove a Filament 126
To Install a Filament 128

8 Operation Manual

9
Agilent Technologies
Agilent 7200 Accurate-Mass Quadrupole Time-of-Flight GC/MS System
Operation Manual
1
Introduction
Abbreviations Used 10
The 7200 Accurate-Mass Quadrupole Time-of-Flight GC/MS System 12
Hardware Description 15
Important Safety Warnings 16
Safety and Regulatory Certifications 20
Intended Use 23
Cleaning/Recycling the Product 23
Liquid Spills 23
Moving or Storing the MS 23
This section provides general information about the 7200
Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) GC/MS
System, including a hardware description, and general safety
warnings.

10 Operation Manual
1 Introduction
Abbreviations Used
Abbreviations Used
The abbreviations in Table 1are used in discussing this
product. They are collected here for convenience.
Table 1 Abbreviations
Abbreviation Definition
AC Alternating current
ALS Automatic liquid sampler
BFB Bromofluorobenzene (calibrant)
CC Collision cell
CI Chemical ionization
DC Direct current
DFTPP Decafluorotriphenylphosphine (calibrant)
EI Electron impact
EPC Electronic pneumatic control
eV Electron volt
GC Gas chromatograph
id Inside diameter
LAN Local Area Network
m/z Mass to charge ratio
MFC Mass flow controller
MS Mass spectrometer
NCI Negative chemical ionization
OFN Octafluoronaphthalene (sample)
PCI Positive chemical ionization
PFDTD Perfluoro-5,8-dimethyl-3,6,9-trioxydodecane (calibrant)
PFTBA Perfluorotributylamine (calibrant)