Agilent Technologies InfinityLab LC Series User manual

Agilent Technologies
Agilent InfinityLab LC Series
1260 Infinity II Binary LC
System
Manual and Quick Guide

1260 Infinity II Binary LC System - Manual and Quick Guide
Notices
© Agilent Technologies, Inc. 2016
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Manual Part Number
G7112-90300
Edition
09/2016
Printed in Germany
Agilent Technologies
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76337 Waldbronn
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1260 Infinity II Binary LC System - Manual and Quick Guide 3
In This Book
In This Book
This book describes the Agilent InfinityLab LC Series 1260 Infinity II Binary
LC system.
1Introduction
This chapter gives an introduction to the Agilent 1260 Infinity II LC, the
underlying concepts and the features of the Agilent 1260 Infinity II LC.
2 Configuration Settings
This chapter describes how to configure the system.
3QuickStartGuide
This chapter provides information on running an Agilent 1260 Infinity II LC.
4 Parts and Consumables
This chapter provides information on additional parts and consumables.
5 Appendix
This chapter provides addition information on safety, legal, and web.

4 1260 Infinity II Binary LC System - Manual and Quick Guide
Contents
Contents
1Introduction 5
Product Description 6
Features of the Agilent 1260 Infinity II Binary LC 7
System Components 8
Solutions 18
Optimizing the Stack Configuration 22
Leak and Waste Handling 28
2 Configuration Settings 35
General Information on LAN Configuration 36
Instrument Configuration 37
Lab Advisor 39
3QuickStartGuide 45
Best Practices 46
Prepare a Run 47
Check Out the System 54
4 Parts and Consumables 65
Tool Kit 66
InfinityLab Quick Connect and Quick Turn Fittings 68
5 Appendix 73
Safety 74
Agilent Technologies on Internet 80

5
1260 Infinity II Binary LC System - Manual and Quick Guide
Agilent Technologies
1
Introduction
Product Description 6
Features of the Agilent 1260 Infinity II Binary LC 7
System Components 8
Binary Pump G7112B 9
Multisampler G7167A 10
Vialsampler G7129A 11
Multicolumn Thermostat G7116A 12
Diode Array Detector HS G7117C 13
Diode Array Detector WR G7115A 14
Variable Wavelength Detector G7114A 15
Fluorescence Detector G7121A 16
Refractive Index Detector G7162A 17
Solutions 18
Walk-up 18
Online SPE 18
1260 Infinity II Multimethod Solution 20
Optimizing the Stack Configuration 22
Agilent InfinityLab LC Flex Bench 22
One Stack Configuration 24
Two Stack Configuration 26
Leak and Waste Handling 28
Waste Guidance 32
Waste Concept 33
This chapter gives an introduction to the Agilent 1260 Infinity II LC, the
underlying concepts and the features of the Agilent 1260 Infinity II LC.

6 1260 Infinity II Binary LC System - Manual and Quick Guide
1 Introduction
Product Description
Product Description
The Agilent 1260 Infinity II LC System is the trusted platform, taking you to
the next level of routine analysis, and giving you the instrument choice to
achieve best operational efficiency.
A broad range of reliable instrumentation matches with latest column
technologies and guarantees robust separation and detection performance.
Highest instrument utilization and a fast turnaround cycle is achieved through
easy column handling and superior sample logistics from sample submission
to data analysis.
The designed for method transferability and stepwise upgrade capability
enables a risk-free integration in current infrastructure matching your budget.

1260 Infinity II Binary LC System - Manual and Quick Guide 7
Introduction
1
Features of the Agilent 1260 Infinity II Binary LC
Features of the Agilent 1260 Infinity II Binary LC
With an operating pressure of up to 600 bar, the 1260 Infinity II Binary Pump
is compatible with HPLC and UHPLC, i.e. handling 2.1, 3, and 4.6 mm ID
columns over the flow rate range (up to 5 mL/min). Gradient formation is
based on a high-pressure mixing principle.
Ultralow carryover – The 1260 Infinity II Multisampler is designed for low
carryover, you can take clean to a whole new level with the multi-wash
capability, cleaning all relevant injection parts between runs. This
sophisticated, integrated feature flushes the injection needle outside with
three solvents, and uses seat back flush procedures to reduce carryover to less
than 9 ppm.
Dual-needle injection – By running samples alternately through one or the
other injection path, you can reduce cycle times to mere seconds, virtually
eliminating conventional wait times—whether for large volume loadings or
flushing procedures.
Higher sample capacity per benchspace – Using shallow well-plate drawers,
the 1260 Infinity II Multisampler takes a maximum load of 16 microtiter plates
and up to 6144 samples—the most of any single system.
Advanced column capacity for up to 4 columns in a single 1260 Infinity II
Multicolumn Thermostat delivering best flexibility for column switching.
Fast and easy connections with InfinityLab Quick Connect fittings to save time
and trouble.
1260 Infinity II DAD HS Detector delivers lower detection limit and higher
data quality for more confidence.
Seamless integration in your chromatography data system: Agilent’s
Instrument Control Framework (ICF) enables smooth control of Agilent LC
instrumentation through third-party chromatography data systems.

8 1260 Infinity II Binary LC System - Manual and Quick Guide
1 Introduction
System Components
System Components
The Agilent 1260 Infinity II Binary LC system consists of the following
components:
•Binary Pump
•Multisampler or Vialsampler
•Multicolumn Thermostat (MCT)
•Diode Array (DAD), Variable Wavelength (VWD), Refractive Index (RID), or
Fluorescence Detector (FLD)
•Evaporative Light Scattering Detector (ELSD) (optional, not stackable)
•Solvent Cabinet
The Agilent 1260 Infinity II Binary LC is described in more detail in the
following sections. All modules (except the optional ELSD) are stackable, see
“Optimizing the Stack Configuration” on page 22.
For specifications, please refer to the individual module user documentation.

1260 Infinity II Binary LC System - Manual and Quick Guide 9
Introduction
1
System Components
Binary Pump G7112B
The Agilent 1260 Infinity II Binary Pump is suited for LC applications using
conventional or superficially porous particles columns, as well as STM column
technology. Gradient formation is based on a high-pressure mixing principle.
Standard or low delay volumes can be easily configured by the user. The
Agilent 1260 Infinity II Binary Pump offers reproducible gradients and
high-performance, providing high-throughput and fast separations. The pump
is suitable for routine applications, with UV or MS detection, where high speed
and resolution with uncompromised data quality are required.
Figure 1 Overview of the pump
Status indicator
Active seal wash
Purge valve
Degasser
Pump head A
Pump head B
Leak drain
Power switch

10 1260 Infinity II Binary LC System - Manual and Quick Guide
1 Introduction
System Components
Multisampler G7167A
The Agilent 1260 Infinity II Multisampler can handle both vials and microtiter
plates with ease and efficiency up to 600 bar system pressure, optimized on
high flexibility.
This compact module can house up to 6144 samples, all inside the Agilent
stack footprint and the robotics to inject each into the chromatograph in turn.
With the multi-wash capability, you can reduce carryover to less than 9 parts
per million.
Figure 2 Overview of the Multisampler
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+RWHOGUDZHU
/HDNGUDLQ
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