Agilent Technologies G7166A User manual

Agilent Technologies
Agilent 1260 Infinity II
Preparative Valve-Based
Fraction Collector
User Manual

Preparative Valve-Based Fraction Collector
Notices
© Agilent Technologies, Inc. 2016-2018
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Manual Part Number
G7166-90000 Rev. B
Edition
03/2018
Printed in Germany
Agilent Technologies
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76337 Waldbronn
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hazard. It calls attention to an
operating procedure, practice, or
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hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
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WARNING notice until the indi-
cated conditions are fully
understood and met.

Preparative Valve-Based Fraction Collector 3
In This Guide
In This Guide
This manual contains technical reference information about the Agilent 1260
Infinity II Preparative Valve-Based Fraction Collector.
1 Introduction to the Valve-Based Fraction Collector
This chapter gives an introduction to the module, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Preparative Valve-based Fraction Collector
This chapter gives information about the installation of the module.
4 Using the Preparative Valve-Based Fraction Collector
This chapter explains the essential operational parameters of the module.
5 Preparing the Preparative Valve-Based Fraction Collector
This chapter explains the operational parameters of the module.
6 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 Preparative Valve-Based Fraction Collector
In This Guide
8 Maintenance
This chapter gives you an overview and instructions about the possible
maintenance and repair procedures that can be performed by the user.
9 Parts and Materials
This chapter provides information on parts and materials.
10 Identifying Cables
This chapter provides information on cables used with the module.
11 Hardware Information
This chapter describes the module in more detail on hardware and electronics.
12 Appendix
This chapter provides additional information on safety, legal, and web.

Preparative Valve-Based Fraction Collector 5
Contents
Contents
1 Introduction to the Valve-Based Fraction Collector 9
Product Description 10
Features 11
Typical Applications 12
Mounting Examples 13
2 Site Requirements and Specifications 15
Site Requirements 16
Physical Specifications 19
Performance Specifications 20
3 Installing the Preparative Valve-based Fraction Collector 21
Unpacking the module 22
Installing the Preparative Valve-Based Fraction Collector 24
Flow connections with the Preparative Valve-Based Fraction Collector 31
4 Using the Preparative Valve-Based Fraction Collector 33
Configuration and Operation of the Fraction Collector 34
Solvent Information 36
Status Indicators 42
5 Preparing the Preparative Valve-Based Fraction Collector 43
Leak and Waste Concept 44
Best Practices 45
Setting up the Preparative Valve-Based Fraction Collector with the Instrument
Control Interface 46
Method Parameter Settings 52
Pooling 62

6 Preparative Valve-Based Fraction Collector
Contents
6 Troubleshooting and Diagnostics 63
User Interfaces 64
Agilent Lab Advisor Software 65
Hardware Symptoms 66
7 Error Information 69
What Are Error Messages 70
General Error Messages 71
Module Specific Error Messages 76
8 Maintenance 79
Introduction to Maintenance 80
Cautions and Warnings 81
Overview of Maintenance 83
Cleaning the Module 84
Replacing the Fuses of the Preparative Valve-Based Fraction Collector 85
Replacing the Module Firmware 87
9 Parts and Materials 89
Valve Drive Parts 90
Accessory Kit 91
10 Identifying Cables 93
Cable Overview 94
Analog Cables 96
Remote Cables 98
CAN/LAN Cables 102
RS-232 Cables 103
USB 104

Preparative Valve-Based Fraction Collector 7
Contents
11 Hardware Information 105
Electrical Connections 106
Interfaces 109
Configuration Switch Settings of the Preparative Valve-Based Fraction
Collector 116
12 Appendix 119
General Safety Information 120
Waste Electrical and Electronic Equipment Directive 127
Radio Interference 128
Sound Emission 129
Agilent Technologies on Internet 130

8 Preparative Valve-Based Fraction Collector
Contents

9
Preparative Valve-Based Fraction Collector
Agilent Technologies
1
Introduction to the Valve-Based
Fraction Collector
Product Description 10
Features 11
Typical Applications 12
Mounting Examples 13
This chapter gives an introduction to the module, instrument overview and
internal connectors.

10 Preparative Valve-Based Fraction Collector
1 Introduction to the Valve-Based Fraction Collector
Product Description
Product Description
The Agilent 1260 Infinity II Preparative Valve-Based Fraction Collector
(G7166A) comprises 11 collection valves, one waste valve and one solvent inlet
port. Solvent coming from a preparative HPLC system can be diverted into
11 different fractions depending on the software/firmware settings. The valves
can then be blown clean by allowing compressed air or nitrogen to be passed
through the same valves.
No fraction racks are required, as collection takes place into larger collection
bottles that can be placed anywhere within reach of the tubing outlets. The
Preparative Valve-Based Fraction Collector can be used in addition to a
fraction collector.
Figure 1 Overview of front
Valve
Clamps (attachable to both sides)
Leak sensor
Leak plane (attachable)

Preparative Valve-Based Fraction Collector 11
Introduction to the Valve-Based Fraction Collector
1
Features
Features
The 1260 Infinity II Preparative Valve-Based Fraction Collector (G7166A)
includes the following features:
•Built-in power supply
•Leak handling with a leak pane and a leak sensor underneath the valve
head
•Flexible mounting bracket, for left- or right-side mounting on LC stacks or
at the Agilent Column Organizer (G1383A)
•Automated delay volume calibration

12 Preparative Valve-Based Fraction Collector
1 Introduction to the Valve-Based Fraction Collector
Typical Applications
Typical Applications
The Preparative Valve-based Fraction Collector allows the user to collect large
fractions from a preparative HPLC run, at high flow rates, while minimizing
carry-over, due to compressed air (or nitrogen) blowing clean the fraction
lines.
The valve’s wetted parts include PEEK and FFKM, which allow for a pH range
of 2 – 13, short term 14
1,2
.
1
For solvent compatibility, refer to section “Solvent information for parts of the 1260 Infinity
Bio-inert LC system” in the Bio-inert system manual.
2
Solvents with a pH of 1, in particular any halogenated acids, can only be used for the cleaning of
each of the ports.

Preparative Valve-Based Fraction Collector 13
Introduction to the Valve-Based Fraction Collector
1
Mounting Examples
Mounting Examples
Figure 2 Mounting Examples for the Preparative Valve-based Fraction Collector

14 Preparative Valve-Based Fraction Collector
1 Introduction to the Valve-Based Fraction Collector
Mounting Examples

15
Preparative Valve-Based Fraction Collector
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 16
Physical Specifications 19
Performance Specifications 20
This chapter provides information on environmental requirements, physical
and performance specifications.

16 Preparative Valve-Based Fraction Collector
2 Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Considerations
The module power supply has wide ranging capability. It accepts any line voltage
in the range described in
Table 1 on page 19. Consequently there is no voltage
selector in the rear of the module. There are also no externally accessible fuses,
because automatic electronic fuses are implemented in the power supply.
WARNING
Hazard of electrical shock or damage of your instrumentation
can result, if the devices are connected to a line voltage higher than specified.
➔Connect your instrument to the specified line voltage only.
WARNING
Electrical shock hazard
The module is partially energized when switched off, as long as the power cord is
plugged in.
The cover protects users from personal injuries, for example electrical shock.
➔Do not open the cover.
➔Do not operate the instrument and disconnect the power cable in case the cover
has any signs of damage.
➔Contact Agilent for support and request an instrument repair service.
WARNING
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument from the
power line at any time.
➔Make sure the power connector of the instrument can be easily reached and
unplugged.
➔Provide sufficient space behind the power socket of the instrument to unplug the
cable.

Preparative Valve-Based Fraction Collector 17
Site Requirements and Specifications
2
Site Requirements
Power Cords
Country-specific power cords are available for the module. The female end of
all power cords is identical. It plugs into the power-input socket at the rear.
The male end of each power cord is different and designed to match the wall
socket of a particular country or region.
Agilent makes sure that your instrument is shipped with the power cord that
is suitable for your particular country or region.
WARNING
Absence of ground connection
The absence of ground connection can lead to electric shock or short circuit.
➔Never operate your instrumentation from a power outlet that has no ground
connection.
WARNING
Unintended use of supplied power cords
Using power cords for unintended purposes can lead to personal injury or damage
of electronic equipment.
➔Never use a power cord other than the one that Agilent shipped with this
instrument.
➔Never use the power cords that Agilent Technologies supplies with this instrument
for any other equipment.
➔Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
WARNING
Power cords
Solvents may damage electrical cables.
➔Prevent electrical cables from getting in contact with solvents.
➔Exchange electrical cables after contact with solvents.

18 Preparative Valve-Based Fraction Collector
2 Site Requirements and Specifications
Site Requirements
Bench Space
The module dimensions and weight (see Table 1 on page 19) allow you to place
the module on almost any desk or laboratory bench. It needs an additional
2.5 cm (1.0 inches) of space on either side and approximately 8 cm
(3.1 inches) in the rear for air circulation and electric connections.
If the bench shall carry a complete HPLC system, make sure that the bench is
designed to bear the weight of all modules.
The module should be operated in a horizontal position.
Condensation
NOTE
Agilent recommends that you install the HPLC instrument in the A-Line Flex Bench rack.
This option helps to save bench space as all modules can be placed into one single stack.
It also allows to easily relocate the instrument to another Lab.
CAUTION
Condensation within the module
Condensation can damage the system electronics.
➔Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
➔If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.

Preparative Valve-Based Fraction Collector 19
Site Requirements and Specifications
2
Physical Specifications
Physical Specifications
Table 1 Physical Specifications
Type Specification Comments
Weight 1.9 kg (4.2 lbs)
Dimensions
(height × width × depth)
95 x 95 × 300 mm
(3.7 x 3.7 × 11.8 inches)
Line voltage 100 – 240 V~, ± 10 % Wide-ranging
capability
Line frequency 50 or 60 Hz, ± 5 %
Power consumption 23 VA / 7 W
Ambient operating
temperature
4 – 40 °C (39 – 104 °F)
Ambient non-operating
temperature
-40 – 70 °C (-40 – 158 °F)
Humidity <80 % r.h. at 4 – 31 °C, decreasing to
50 % r.h. at 40 °C
Non-condensing
Operating altitude Up to 3000 m (9842 ft)
Non-operating altitude Up to 4600 m (15092 ft) For storing the module
Safety standards:
IEC, EN, CSA, UL
Installation category II, Pollution degree 2 For indoor use only.
ISM Classification ISM Group 1 Class B According to CISPR 11

20 Preparative Valve-Based Fraction Collector
2 Site Requirements and Specifications
Performance Specifications
Performance Specifications
Table 2 Agilent 1260 Infinity II Preparative Valve-Based Fraction Collector (G7166A)
Performance Specifications
Type Specification
Number of solvent channels 13 (including inlet and waste)
Fittings ¼-28
External leak sensor YES, same function as internal sensor
Solvent Flow range 0 – 200 mL/min per channel
Operating pressure (gas and solvent) 2 bar (29 psi) per channel
Maximum pressure (gas and solvent) 2.5 bar (36 psi) per channel
Gas Specification Dry filtered compressed air or nitrogen
Gas Flow Range 0.9 – 3.25 SLM (controlled gas shut-off)
Maximum Inlet Gas Pressure 7 bar (101 psi)
Internal volume per channel 20 µL per channel
Fuse 2.0 A / 250 V High breaking capacity fuse,
exchangeable
Materials in contact with solvent PEEK, FFKM
pH range 2 – 13, short term 14
1,2
1
For solvent compatibility, refer to section “Solvent information for parts of the 1260 Infinity Bio-in-
ert LC system” in the Bio-inert system manual.
2
Solvents with a pH of 1, in particular any halogenated acids, can only be used for the cleaning of
each of the ports