Agilent Technologies InfinityLab LC Series User manual

Agilent Instant Pilot G4208
Agilent InfinityLab LC Series
User’s Guide
G4208A User’s Guide

G4208A User’s Guide
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Document No: SD-29000124 Rev. B
Part No: G4208-90010 Rev. B
EDITION 04/2020
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G4208A User’s Guide 3
In This Guide...
This book describes how to operate the Agilent 1100/1200/1260/1290 Series
modules and systems for HPLC using the handheld control module Agilent Instant
Pilot G4208A.
The Instant Pilot provides complete local control and monitoring of a single
module or an entire Agilent 1100/1200/1260 and 1290 Series HPLC system. There
is no data evaluation in the Instant Pilot. The Instant Pilot allows you to do a variety
of HPLC tasks including automated sample preparation and injection, isocratic,
gradient and multiple method analyses and basic diagnostics and maintenance.
1Start-upInformation
This chapter provides general information around the Agilent Instant Pilot.
2 Working with the Instant Pilot
This chapter describes the operation of the Instant Pilot.
3 Running an Isocratic Analysis
This chapter describes how to analyze the Agilent Technologies isocratic standard
sample using a single injection analysis.
4 Running Multiple-Vial Analyses
This chapter describes how to setup multiple vial analyses using the same method
and different methods.
5 Maintenance and Repair
This chapter describes how to perform firmware updates, troubleshooting and
replacements.
6Appendix
This chapter provides safety and other general information.
NOTE
If additional details are required on a specific topic/function/parameter not
mentioned in this document, please use the Instant Pilot’s Online Information
System (i), see “The i (info) key - Online Information System” on page 23.

G4208A User’s Guide 4
Contents
1Start-upInformation7
Instant Pilot Features 8
Features and Benefits 10
Requirements for the Instant Pilot 11
Physical Specifications 13
Cleaning 14
Holder Versions for the Instant Pilot 15
Adding the Instant Pilot to an Agilent HPLC Module 16
Removing the Instant Pilot 20
Instant Pilot Display and Keyboard Layout 21
The i (info) key - Online Information System 23
Basic Operational Concept of the Instant Pilot 26
Getting Started 27
System Information 33
Method Information 34
Sequence Information 36
Status Information 38
Logbook Information 43
Configuration 45
Maintenance Information 46
Diagnosis Information 50
Turning Modules ON/OFF/Standby 51
Start Analysis Screen 52
Switching from G1323A/B Control Module to Instant Pilot 53
Information on Firmware 55

G4208A User’s Guide 5
2 Working with the Instant Pilot 56
Using a USB Flash Drive 57
Printing To USB Flash Drive 59
Working with Methods 64
Sequence - Automating Analyses 84
Displaying Data Graphically 95
DAD/MWD/VWD/FLD Spectrum 98
Connecting External Devices 101
Simultaneous Execution with Software 104
Special Functions 107
3 Running an Isocratic Analysis 108
What You Will Need 109
Preparing the LC System 110
Entering Settings 111
Saving Settings in a Method 112
Creating a Sequence 113
Selecting a Signal 114
Observing the Chromatogram 115
4 Running Multiple-Vial Analyses 116
Analyzing Multiple Vials Using the Same Method 117
Analyzing Multiple Vials Using Different Methods 118
Single-Level Calibration Sequences 120
Multiple-Level Calibration Sequences 123
Re-calibrating With Multiple Groups of Standards 126
Synchronizing Analyses with External Devices 129

G4208A User’s Guide 6
In This Guide...
5 Maintenance and Repair 135
Firmware Updates 136
Troubleshooting 150
Repairing the Instant Pilot 152
Exchanging the CAN Cable 153
6Appendix157
Safety Information 158
Waste Electrical and Electronic Equipment (WEEE) Directive 160
Radio Interference 161
Agilent Technologies on Internet 162

G4208A User’s Guide 7
1Start-upInformation
Instant Pilot Features 8
Features and Benefits 10
Requirements for the Instant Pilot 11
Physical Specifications 13
Cleaning 14
Holder Versions for the Instant Pilot 15
Adding the Instant Pilot to an Agilent HPLC Module 16
Adding the Instant Pilot to an Agilent HPLC Module (1100/1200/1200 Infinity Series) 17
Adding the Instant Pilot to an Agilent HPLC Module (1200 Infinity II Series) 18
Removing the Instant Pilot 20
Instant Pilot Display and Keyboard Layout 21
The i (info) key - Online Information System 23
Basic Operational Concept of the Instant Pilot 26
Getting Started 27
System Information 33
Method Information 34
Sequence Information 36
Status Information 38
Setup of a Status Information Screen 40
Logbook Information 43
Configuration 45
Maintenance Information 46
Early Maintenance Feedback (EMF) 48
Product Number and Serial Number Change 49
Diagnosis Information 50
Turning Modules ON/OFF/Standby 51
Start Analysis Screen 52
Switching from G1323A/B Control Module to Instant Pilot 53
Information on Firmware 55
This chapter provides general information around the Agilent Instant Pilot.

G4208A User’s Guide 8
1 Start-up Information
Instant Pilot Features
Instant Pilot Features
Figure 1 The Agilent Instant Pilot
The Agilent Instant Pilot (IP) provides complete local control and monitoring of a
single module or an entire Agilent 1100/1200/1260/1290 Series HPLC system.
You have easy access to every supported function, you can easily control all
parameters and settings and you can configure various communication channels
with other devices, in order to comfortably analyze the generated data.
•Color display, size 13.1 x 9.9 cm (5.0 x 3.8 inch), 640 x 480 dots
•Install any desired configuration of Agilent 1100/1200/1260/1290 Series
HPLC modules. The Instant Pilot software will reflect which modules are
present in the LC system and adjust the screens accordingly.
•Enter parameter settings for every module, perform on/off functions as well
as calibration and configuration settings in a self- explanatory and intuitive
way.
•Define automated analyses including methods, timetables, method
sequences and automated calibration settings using the Instant Pilot.
•Use the configurable status screen to monitor various activities on a single
screen.
•Easy configuration of the system and/or modules.

G4208A User’s Guide 9
1 Start-up Information
Instant Pilot Features
•Protect your method from any inadvertent keyboard changes by setting
method file protection.
•Use USB Flash Drive to store and transfer methods and sequences between
Agilent systems.
•Monitor all operations and error events using the self- updating logbooks.
•Use the context- sensitive online information system to get further
information on all topics.
•To help comply with Good Laboratory Practice (GLP) regulations, select a
variety of module tests that will check the performance of the LC system.
•Use the early maintenance feedback (EMF) limits for scheduling maintenance
work.
•Display data graphically using the Plot screen, where as many as four
different signals can be monitored at the same time.
•Printing to an USB Flash Drive.
•Version A.05.15 is compatible with 1100/1200 modules that run on firmware
A.05.11/13 and A.05.09/10 (introduced November 2006).
NOTE
Firmware revision A.05.13/14 does not run on Instant Pilot modules with serial
numbers starting with MY due to new flash ROM type that does not allow
downgrades to B.02.07 and below. This was fixed with firmware A.05.15 and
B.02.15.

G4208A User’s Guide 10
1 Start-up Information
Features and Benefits
Features and Benefits
Table 1 Features and Benefits
Feature Benefit
•Large size, color display with background light, high reso-
lution and contrast
Better readability and usability.
•USB port / USB Flash Drive Faster and more flexible method and sequence transfer to
other Agilent systems. Handling for unsupported formats /
formatting
•State of the art electronic Faster application, large number of Agilent modules connect-
able, all detector signals available in plot.
•System visualization with status display in start screen Fast overview of configuration and state of system
•Flat dialog structure; easy to understand icons Much faster confidence and usability, less training required
•Automatic, context sensitive help in status line ('Tool Tip') Easier parameter input through given ranges
•Diagnosis with passed/failed No user interpretation necessary, clear result
•Setup wizards Easier system configuration and sequence setup
•Dynamic adjustment to changed system configuration No restart necessary when system configuration changes,
e.g. different detector
•Method on- and offline editable Methods can be changed during runs
•New sequence: wizard, table view, priority sample, insert
method, parameter, ...
Simpler and more flexible, better overall view
•Printing to USB Flash Drive The files can be opened using Microsoft Internet Explorer and
printed from there.

G4208A User’s Guide 11
1 Start-up Information
Requirements for the Instant Pilot
Requirements for the Instant Pilot
The Agilent Instant Pilot can be attached to a modular Agilent HPLC system or a
single Agilent HPLC module. Depending on the system, the following firmware
requirements must be fulfilled.
Table 2 Pre-requisites / Compatibility vs. Modules
Agilent HPLC Modules Instant Pilot Firmware
Revision
A.05.13/14/15
Instant Pilot Firmware
Revision
B.01.02/03/04
Instant Pilot Firmware
Revision B.02.01 and
above
1260/1290 Infinity II Series not compatible not compatible B.02.17 or above
1260 Infinity Series not compatible not compatible B.02.11 or above
1220 Infinty Series LC Systems not compatible not compatible not compatible
1290 Infinity System
•G4212A DAD
•G4220A Binary Pump
•G4226A Autosampler
•G1316C TCC
not compatible not compatible B.02.08 or above
1120 Compact LC not compatible not compatible not compatible
Newer Series 1100/1200 modules that
required special versions of Instant Pilot
•G1315C DAD-SL not compatible B.01.02 or above B.01.02 or above
•G1365C MWD-SL not compatible B.01.02 or above B.01.02 or above
•G1315D DAD not compatible not compatible B.01.02 or above
•G1365D MWD not compatible not compatible B.01.02 or above
•G1314D VWD not compatible not compatible B.01.07 or above
•G1314E VWD SL Plus not compatible not compatible B.01.07 or above
•G1367D ALS SL Plus) not compatible not compatible B.01.07 or above
1100/1200/1260 ChipCube (G4240A) not compatible not compatible not compatible
Series 1200 standard modules (includes all
modules not mentioned below)
A.05.09/10
A.05.11/12/13
A.06.02 or above A.06.02 or above
Series 1100 standard modules (includes all
modules not mentioned below)
A.05.09/10
A.05.11/12/13
A.06.02 or above A.06.02 or above

G4208A User’s Guide 12
1 Start-up Information
Requirements for the Instant Pilot
NOTE
Since USB Flash Drives may vary from vendor to vendor or from type to type,
incompatibilities can occur. In general, USB Flash Drives from Sandisk and
Kingston should work. The USB Flash Drive must be FAT-16 formatted and
without encryption. See Table 14 on page 152
CAUTION
The operator of this instrument is advised that if the equipment is used in a
manner not specified in this manual, the protection provided by the equipment
may be impaired.
Use the instrument in a manner only, that is specified in this manual.
NOTE
The Instant Pilot may be used only with the Agilent instruments.
NOTE
The Instant Pilot is designed to operate in a typical electromagnetic environment
(EN61326-1) where RF transmitters, such as mobile phones, should not be used
in close proximity.

G4208A User’s Guide 13
1 Start-up Information
Physical Specifications
Physical Specifications
Table 3 Physical Specifications
Type Specification Comments
Weight 0.8 kg (1.76 lbs)
Dimensions
(width × depth × height)
130 × 225 × 35 mm
(5.1 × 8.9 × 1.4 inches)
Line voltage 22 VDC, ± 10 % via CAN
Power consumption 7 W
Ambient operating temperature 4 – 55 °C (39 – 131 °F )
Ambient non-operating temperature -40 – 70 °C (-40 – 158 °F)
Humidity 95 %, at 25 – 40 °C (77 – 104 °F) Non-condensing
Operating altitude Up to 3000 m (9842 ft)
Non-operating altitude Up to 4600 m (15092 ft) For storing
Safety standards: IEC, CSA, UL, EN Installation category II, pollution
degree 2.
For indoor use only.
Research Use Only. Not for use in
Diagnostic Procedures.
NOTE
This product with serial numbers DE and MY16355458 and below contains an
TFT LCD assembly which is backlit by a mercury fluorescent lamp which
contains mercury, and must be managed, recycled, and/or disposed in
accordance with all applicable laws, ordinances, and regulations. For information
on how to recycle or dispose of the mercury lamp contained in this product, or if
you have additional questions on the mercury contained within this product,
contact Agilent customer service.
NOTE
Starting with MY16375501 the product is RoHS compliant and uses a LED
display (which does not contain mercury) that requires normal local disposal
regulations.

G4208A User’s Guide 14
1 Start-up Information
Cleaning
Cleaning
To keep the module case clean, use a soft cloth slightly dampened with water, or
a solution of water and mild detergent.
WARNING
Liquid dripping into the electronic compartment of your module can cause
shock hazard and damage the module
Do not use an excessively damp cloth during cleaning.
Drain all solvent lines before opening any connections in the flow path.

G4208A User’s Guide 15
1 Start-up Information
Holder Versions for the Instant Pilot
Holder Versions for the Instant Pilot
Mid of 2007, the holder of the Instant Pilot was introduced with a revised design.
Features of new holder are
•easy to use
•safe placement of the Instant Pilot
•stable mechanism
•easy upgrade of all Instant Pilot’s with old holder version possible (see “Parts
Identification” on page 152).
Figure 2 on page 15 shows all holder versions. In the actual versions, the Instant
Pilot is hanging in the new holder and standing in the obsolete holder.
Figure 2 Holder Versions
original 1100 design (obsolete)
1100/1200/1200 Infinity design
1200 Infinity II design

G4208A User’s Guide 16
1 Start-up Information
Adding the Instant Pilot to an Agilent HPLC Module
Adding the Instant Pilot to an Agilent HPLC
Module
To attach the Instant Pilot to an Agilent HPLC module, the provided adapter plate
is required.
CAUTION
The CAN connectors are similar to LAN adapter connectors
Do not insert LAN connectors into the CAN or vice versa, since the CAN
uses 24 V and might damage the LAN card.
CAUTION
The CAN bus does not support hot plugging or unplugging.
Unwanted or unexpected behavior of the instrument.
Switch off all HPLC modules, before plugging/unplugging the Instant Pilot.
NOTE
Preferred orientation of the Instant Pilot is hanging at the HPLC modules.
NOTE
When inserting the adapter to an Agilent Wellplate Autosampler or Fraction
Collector, the Instant Pilot must be removed first. Otherwise the Autosampler
door cannot be opened.

G4208A User’s Guide 17
1 Start-up Information
Adding the Instant Pilot to an Agilent HPLC Module
Adding the Instant Pilot to an Agilent HPLC Module
(1100/1200/1200 Infinity Series)
1Slide the adapter plate (delivered with the Instant Pilot)
from the front onto the top cover of the Agilent HPLC
module.
2Assure that it is fixed by pressing onto the adapter
plate.
3Insert the Instant Pilot into the holder. 4Insert the Instant Pilot into the holder.
5The Instant Pilot in it’s final position. 6Connect the CAN cable of the Instant Pilot into a free
CAN connector at the rear of the module.

G4208A User’s Guide 18
1 Start-up Information
Adding the Instant Pilot to an Agilent HPLC Module
Adding the Instant Pilot to an Agilent HPLC Module
(1200 Infinity II Series)
Preparations Content of Holder Infinity II Kit
The kit 5067- 5955 (part of the Instant Pilot G4208A from May 2015) includes
•Holder Infinity II
•Screw
•Installation Note
Procedure
Refer to the User Manual of your Infinity II module.
1Remove the right door of module. 2Remove the top leak panel.
3The holder must be clipped into the top cover. 4Insert the nose in the bottom of the cover into the slit in
the holder and fit the holder on the cover.

G4208A User’s Guide 19
1 Start-up Information
Adding the Instant Pilot to an Agilent HPLC Module
5Fix the holder with the screw. 6Re-install the Leak Interface Top and the door.
7Insert the Instant Pilot into the holder. 8Connect the CAN cable of the Instant Pilot into a free
CAN connector at the rear of the module.

G4208A User’s Guide 20
1 Start-up Information
Removing the Instant Pilot
Removing the Instant Pilot
1To remove the Instant Pilot, slightly angle the Instant Pilot (1.) and then lift it
from the holder (2.).
Figure 3 Removing the Instant Pilot
CAUTION
The CAN bus does not support hot plugging or unplugging.
Unwanted or unexpected behavior of the instrument.
Switch off all HPLC modules, before plugging/unplugging the Instant Pilot.
1.
2.