Agilent Technologies Triple Quadrupole LC/MS User manual

Agilent Triple Quadrupole LC/MS System
User Guide

Notices
Document Identification
D0037077 Revision A.00
October 2023
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© Agilent Technologies, Inc. 2023
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Safety Notices
CAUTION
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Software Revision
This guide is valid for MassHunter 12.1,
until superseded.
Instrument Manufacturing
Manufactured by Agilent
Technologies Singapore Pte. Ltd.
No. 1 Yishun Avenue 7, Singapore
768923
Operating Temperature
Operating Temperature: 15 °C to 35 °C
Storage Temperature: -40 °C to 70 °C

Agilent Triple Quadrupole LC/MS System User Guide 3
In This Guide
This guide provides information on the Ultivo, 6475A, and 6495D LC/TQ systems
running MassHunter Data Acquisition 12.1 or higher.
Additional Resources
User Documentation
Data analysis and library management documentation can be found by scanning
the code or navigating to https://aglt.co/DALibMgmtDocs.
Instrument documentation, step by step videos, and more can be found by
scanning the code or navigating to https://aglt.co/LCMSUserDocs.
Agilent Triple Quadrupole LC/MS Supplies
Make sure that you don’t run out of essential columns and supplies. Use this quick
reference list to keep your shelves stocked by navigating to
https://aglt.co/LCTQSupplies.

4 Agilent Triple Quadrupole LC/MS System User Guide
Technical Support
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Agilent Triple Quadrupole LC/MS System User Guide 7
Content
Technical Support 4
1Introduction 12
Sustainability Information 13
Packaging End-of-Life 13
Product End-of-Life 13
Agilent Regulatory Compliance Statement 14
CE Compliance 14
UK Compliance 15
Electromagnetic Compatibility 16
EMC Declaration for South Korea 17
Detachable Power Cord Declaration for Japan 17
Sound Emission Certification for Federal Republic of Germany 17
Waste Electrical and Electronic Equipment (WEEE) Directive 18
2 Hardware Overview 20
Ultivo Triple Quadrupole LC/MS 21
Front view 21
Rear view 22
Calibrant Delivery System 23
6475A Triple Quadrupole LC/MS 24
Front view 24
Side view 25
Calibrant Delivery System 26
6495D Triple Quadrupole LC/MS 27
Front view 27
Side view 28
Calibrant Delivery System 29
Magnets 30

Content
Agilent Triple Quadrupole LC/MS System User Guide 8
Instrument Status Indicator Lights 33
Instrument Status Indicators in Acquisition 34
Ion Sources 35
Electrospray Ionization (ESI) source 35
Agilent Jet Stream (AJS ESI) source 36
Atmospheric Pressure Chemical Ionization (APCI) source 37
Multimode Ionization (MMI) source 38
Nanospray ESI source 39
3Tuning 40
About Tuning 41
Autotune and checktune 42
Starting the Instrument 43
Calibrating the LC/TQ (Checktune) 44
6475A and Ultivo 44
6495D 45
Configure Tune - (6495D only) 46
Calibrating the LC/TQ (Autotune) 48
6475A and Ultivo 48
6495D 49
Generating a Detailed Tune Report 50
6495D 51
Scheduling a Checktune 52
Scheduling an Autotune 54
Advanced Tune - 6495D 56
Diagnostic Tune - 6495D only 57

Content
Agilent Triple Quadrupole LC/MS System User Guide 9
4 Basic Operation 58
Overview 60
Starting Up and Shutting Down the Instrument 61
Starting in Standby mode 61
Turning off the instrument 61
Starting the Mass Hunter Data Acquisition Software 63
MassHunter Data Acquisition windows 64
Instrument Status window 65
Actuals window 65
Chromatogram Plot window 66
Spectrum window 67
Method Editor window 67
Sample Run window 68
Worklist window 68
Method Optimizer window 69
dMRM Method Split window 69
Preparing the LC Modules 70
Switch LC stream to Waste 70
Preparing the Triple Quad LC/MS 74
Performing a checktune or autotune 74
Switching LC stream to MS 74
Monitoring MS baseline and spectral displays 74
Viewing the System Activity Log for events and errors 75

Content
Agilent Triple Quadrupole LC/MS System User Guide 10
Setting Up an Acquisition Method 76
Setting Up and Running a Single Sample 80
Setting Up and Running a Worklist 81
Reviewing Results with Qualitative Analysis 85
Analyzing Data with Quantitative Analysis 86
5 Maintenance 88
Maintenance Overview 90
Routine Tasks 91
Cleaning the spray chamber - daily 91
Checking and filling the calibrant bottle - weekly 92
Cleaning the spray chamber - weekly 93
Flushing the nebulizer - weekly 96
Periodic Tasks 98
Checking the rough pump oil level 98
Draining and replacing the MS40+ pump oil and replacing the filter 99
Draining and replacing the MS120+ pump oil 101
Replacing the MS120+ pump filter 103
Replace Oil Mist/Exhaust Filter - MS120+ 104
Replacing the nitrogen gas filters 105
General Maintenance Tasks 107
Installing or removing the nebulizer 107
Inspecting the nebulizer 109
Adjusting or replacing the nebulizer needle 111
Installing the capillary 113
Removing the capillary (system with a VacShield) 115
Cleaning the capillary 116
Cleaning a clogged capillary 118
Flushing the Calibrant Delivery System 119
Replacing the electron multiplier horn (Ultivo only) 121
Replacing the MS selection valve rotor seal (Ultivo only) 122
Cleaning the corona needle 123

Content
Agilent Triple Quadrupole LC/MS System User Guide 11
Replacing the corona needle 124
Disinfection 125
Source Maintenance 126
Changing to a source that uses a different tuning mix 126
Installing and removing sources 126
Electrospray Ionization (ESI) source 128
Agilent Jet Stream (AJS ESI) source 130
Atmospheric Pressure Chemical Ionization (APCI) source 132
Multimode Ionization (MMI) source 134

Agilent Triple Quadrupole LC/MS System User Guide 12
1Introduction
Sustainability Information 13
Packaging End-of-Life 13
Product End-of-Life 13
Agilent Regulatory Compliance Statement 14
CE Compliance 14
UK Compliance 15
Electromagnetic Compatibility 16
EMC Declaration for South Korea 17
Detachable Power Cord Declaration for Japan 17
Sound Emission Certification for Federal Republic of Germany 17
Waste Electrical and Electronic Equipment (WEEE) Directive 18