Agilent Technologies AssayMAP 96AM Operating manual


AssayMAP 96AM
Wash Station
Maintenance Guide

Notices
© Agilent Technologies, Inc. 2018
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United States and international copyright
laws.
Installation Guide Part Number
G5542-90004
Edition
Revision B, February 2018
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fully understood and met.

1
AssayMAP 96AM Wash Station
Maintenance Guide

96AM Wash Station Maintenance
Guide
This guide contains the following topics:
•“About this guide” on page 2
•“About the 96AM Wash Station” on page 4
•“Required resources and workflow” on page 4
•“Determining if the chimneys are in good condition” on page 6
•“Emptying the 96AM Wash Station” on page 6
•“Uninstalling the 96AM Wash Station” on page 10
•“Replacing damaged chimneys” on page 11
•“Installing the wash station and connecting the tubing” on
page 13
•“Testing the 96AM Wash Station” on page 16

1 96AM Wash Station Maintenance Guide
About this guide
2AssayMAP 96AM Wash Station Maintenance Guide
About this guide
Who should read this guide
The instructions in this guide assume that you have the following:
•An understanding of the AssayMAP Bravo Platform
•Familiarity with the Protein Sample Prep Workbench
•Experience in working on mechanical equipment
What this guide covers
This guide describes how to inspect and replace chimneys in the 96AM Wash
Station.
Figure 96AM Wash Station
The 96AM Wash Station is a component of the AssayMAP autofilling station, as
the following figure shows.
Figure AssayMAP autofilling station, including 96AM Wash Station, Pump Module, tubing, and carboys
Source Waste

3
1 96AM Wash Station Maintenance Guide
About this guide
AssayMAP 96AM Wash Station Maintenance Guide
Related user guides and where to find them
Accessing the user documentation
You can access the user documentation in the Protein Sample Prep Workbench
as follows:
•Application user guides. In the Protein Sample Prep Workbench, click Utility
Library, locate the utility of interest, and then click Instructions.
•Utility instructions. In the Protein Sample Prep Workbench, click Literature
Library, locate the topic of interest, and then click Open.
Alternatively, you can access the user documentation in the VWorks Knowledge
Base using either of the following methods:
•From within VWorks software, select Help > Knowledge Base or press F1.
•From the Windows desktop, do one of the following:
–Windows 10. Select Start > All Apps > Agilent Technologies > VWorks
Knowledge Base.
–Windows 7. Select Start > All Programs > Agilent Technologies > VVWorks >
User Guides > VWorks Knowledge Base.
•Go to www.agilent.com/chem/askb.
For information on… See…
Potential safety hazards and how to
avoid them
Bravo Platform Safety and
Installation Guide
Using the Protein Sample Prep
Workbench
Literature Library in the Protein
Sample Prep Workbench
Setting up, operating, maintaining, and
troubleshooting the Bravo Platform
Bravo Platform User Guide
Setting teachpoints for the AssayMAP
Bravo Platform
Bravo Platform User Guide
Setting up the Pump Module and the
autofilling reservoirs and wash stations
Pump Module User Guide
Creating and editing VWorks protocols
and application interfaces
VWorks Automation Control User
Guide
Running a syringe test of the Bravo
96AM Head
AssayMAP Syringe Test Utility
User Guide in the Protein Sample
Prep Workbench
Replacing a defective syringe in the
Bravo 96AM Head
AssayMAP Bravo Syringe
Replacement User Guide

1 96AM Wash Station Maintenance Guide
About the 96AM Wash Station
4AssayMAP 96AM Wash Station Maintenance Guide
About the 96AM Wash Station
The 96AM Wash Station has 96 chimneys and is scaled to work with the
syringe probes in the Bravo 96AM Head. The wash station is used for washing
the syringes and washing cartridges or pipette tips that are seated on the
syringe probes.
Figure Chimney detail in the 96AM Wash Station
Fresh wash liquid enters the 96AM Wash Station through two inlet ports (1),
shown in the following figure. The wash liquid flows up through the chimneys,
and the waste overflows from the chimneys and is removed through two outlet
ports (2).
Figure Inlet ports (1) and outlet ports (2) in the 96AM Wash Station
Required resources and workflow
Ensure that you have the following tools and materials:
•Replacement Wash Station Chimneys: large internal diameter, pack 100,
Agilent part number 15586- 002
•Hex driver to uninstall and install 2- mm hex screws
•1 needle- nose pliers to remove the bad chimneys from the 96AM Wash
Station
•Small rubber pad or foam to protect your thumb when seating a new
chimney in the 96AM Wash Station.
CAUTION Ensure that you use only the 96AM Wash Station on the AssayMAP Bravo
Platform. Using a model of the Tip Wash Station other than the 96AM Wash Station can result
in a potential crash with the Bravo 96AM Head.
WARNING The probes of the Bravo 96AM Head are sharp and can scratch you if they
brush across your hand. A probe scratch can expose you to any contaminants remaining on
the probes. Wear gloves and use extreme caution to avoid brushing against the probes.

5
1 96AM Wash Station Maintenance Guide
Required resources and workflow
AssayMAP 96AM Wash Station Maintenance Guide
Perform the following steps in the order given.
Step For this task... See...
1Inspect the chimneys for damage and
irregularities.
“Determining if the
chimneys are in good
condition” on page 6
2Empty the wash station. “Emptying the 96AM Wash
Station” on page 6
3Uninstall the wash station. “Uninstalling the 96AM
Wash Station” on page 10
4Replace the damaged chimneys. “Replacing damaged
chimneys” on page 11
5Install the wash station. “Installing the wash station
and connecting the tubing”
on page 13
6Verify the wash station teachpoint
and adjust it, as necessary:
aInitialize the AssayMAP 1
profile and adjust the
teachpoint for the 96AM Wash
Station.
bUse the AssayMAP Teachpoint
Update protocol to update the
teachpoint in the remaining
profiles in the Protein Sample
Prep Workbench
automatically.
AssayMAP Bravo Platform
Installation Guide (for field
service personnel) or
Bravo Platform User Guide
7Start up the AssayMAP Bravo
Platform and verify that all the
chimneys work consistently.
“Testing the 96AM Wash
Station” on page 16

1 96AM Wash Station Maintenance Guide
Determining if the chimneys are in good condition
6AssayMAP 96AM Wash Station Maintenance Guide
Determining if the chimneys are in good condition
During normal operation of the 96AM Wash Station, the fluid flow out of the
top of each chimney and the shape of the fluid dome at the top of each
chimney must consistent. To ensure consistency in the fluid flow from each
chimney, the chimneys must be:
•Clean and free of any debris.
•Free of deformities or irregularities.
See the good condition chimneys in the following figure. The chimneys
must not be damaged, such as those shown in the figure (possibly from a
head collision).
•Equal in height and installed in the same orientation.
Figure Close up views of 96AM Wash Station chimneys
Chimney irregularities or damaged chimneys can cause large droplets to cling
to the syringe probes, which can introduce variability in the assay.
Emptying the 96AM Wash Station
This topic describes how to use the Pump Reagent task in Bravo Diagnostics to
empty any fluid from the wash station.
Before you start
WARNING The probes of the Bravo 96AM Head are sharp and can scratch you if they
brush across your hand. A probe scratch can expose you to any contaminants remaining on
the probes. Wear gloves and use extreme caution to avoid brushing against the probes.
WARNING When you initialize the Bravo Platform, the head and tie bar can move. To
prevent potential injury, keep clear of the device while it is in motion.
CAUTION To prevent potential equipment damage, ensure that the deck is clear of any
obstacles before initializing the AssayMAP Bravo Platform.

7
1 96AM Wash Station Maintenance Guide
Emptying the 96AM Wash Station
AssayMAP 96AM Wash Station Maintenance Guide
Running the Pump Reagent task to empty the wash station
To empty the wash station using the Pump Reagent task:
1At the carboy that contains the wash solution, remove the lid and lift it so
that the tubing is above the liquid level.
2Ensure that the Protein Sample Prep Workbench is closed, and then start
the VWorks software.
You can use the VWorks desktop icon or the Start menu on the Windows
desktop to start the VWorks software.
3Ensure that Simulation is off.
4In the VWorks window, open the AssayMAP Bravo device file
(AssayMAP Bravo.dev).
To do this, click File > Open. In the Open dialog box, ensure the file type is
Device Files (*.dev), select the file, and then click Open.
The device files are installed in the following default location:
C:\VWorks Workspace\Workbench\Device Files
5Click No when the Would you like to initialize devices now? message appears.
6In the AssayMAP Bravo.dev tab, click Device diagnostics. The Bravo Diagnostics
dialog opens.
CAUTION Using an incorrect profile can cause equipment damage. Ensure that the profile
is correct for the head type and deck configuration.

1 96AM Wash Station Maintenance Guide
Emptying the 96AM Wash Station
8AssayMAP 96AM Wash Station Maintenance Guide
Figure AssayMAP Bravo.dev tab and Profile setting for AssayMAP 1
7Click the Profiles tab, select the Profile name AssayMAP 1, and then click
Initialize this profile.
8If the There appears to be a plate present error message appears, click Ignore and
Continue to continue the homing process.
9When the Verify that it is safe to home the W-axis message appears, click Retry to
continue homing the w-axis.

9
1 96AM Wash Station Maintenance Guide
Emptying the 96AM Wash Station
AssayMAP 96AM Wash Station Maintenance Guide
10 Click the Processes tab and run the Pump Reagent task as follows:
aSelect the following:
bClick Execute command. The wash station empties.
Setting Value
Location 1
Labware at selected location 96AM Tip Wash Station
Command to execute Pump Reagent
Pump Reagent properties
Reservoir mode Fill
Pump speed (0–100%) 50%
Pump on time (1–600 s) 60 seconds
How often 1
Run second pump Yes
Second pump speed (0–100%) 60%
Use weigh station No

1 96AM Wash Station Maintenance Guide
Uninstalling the 96AM Wash Station
10 AssayMAP 96AM Wash Station Maintenance Guide
Uninstalling the 96AM Wash Station
To uninstall the 96AM Wash Station:
1On the side of the Bravo Platform, press the power switch to the off (o)
position.
2Gently, move the Bravo head manually to position it over deck location 6,
which will provide unobstructed access to the wash station at deck
location 1.
Figure AssayMAP Bravo Platform with head positioned at deck location 6
3On the left side as you face the front of the AssayMAP Bravo Platform,
loosen the two 2- mm screws using a hex driver. Uninstall the bracket that
secures the wash station to the platepad.
Figure 96AM Wash Station secured with platepad brackets
4On the opposite side of the platepad, guide the wash station horizontal slot
off the platepad inner bracket.
WARNING To prevent potential injury, turn off the Bravo Platform before you install or
remove any accessory.

11
1 96AM Wash Station Maintenance Guide
Replacing damaged chimneys
AssayMAP 96AM Wash Station Maintenance Guide
5Lift the wash station off of the platepad.
For ease of handling, you may disconnect the tubing from the wash station
using the quick- disconnect fittings.
Replacing damaged chimneys
To replace the damaged chimneys:
1Using needle- nose pliers, pull out the defective chimneys from the wash
station.
IMPORTANT Ensure that you discard any chimneys that you remove.
Using pliers on a chimney will permanently damage the chimney.
2Inspect the wash station hole in which the chimney was seated. Ensure
that the hole is empty and clean.
If necessary, clean the hole.
3Orient the new plastic chimney so that the end with the little dot is at the
top, and then insert the chimney into the hole in the wash station.
Figure Dot on chimney oriented at top end for installation

1 96AM Wash Station Maintenance Guide
Replacing damaged chimneys
12 AssayMAP 96AM Wash Station Maintenance Guide
4Using your thumb, press down on the chimney so that the chimney touches
the bottom surface of the wash station and is fully seated.
You can insert a rubber pad between your thumb and the chimney to
protect your thumb.
5Ensure that all the chimneys in the wash station are oriented so that the
dots are at the top end of the wash station. Ensure that the tops of the
chimneys are level with each other.
Figure Chimney dots oriented at top and tops of chimneys aligned
6Repeat steps 1 to 5 to replace any other defective chimneys.

13
1 96AM Wash Station Maintenance Guide
Installing the wash station and connecting the tubing
AssayMAP 96AM Wash Station Maintenance Guide
Installing the wash station and connecting the tubing
Installing the 96AM Wash Station
Before you start
The following procedure assumes that the platepad with brackets is already
installed at deck location 1.
Figure 96AM Wash Station installed at deck location 1
To install the wash station:
1At deck location 1, ensure that the outer side bracket (next to the
crosshairs) is removed from the platepad.
Figure Bracket next to platepad crosshairs
WARNING To prevent potential injury, turn off the Bravo Platform before you install or
remove any accessory.

1 96AM Wash Station Maintenance Guide
Installing the wash station and connecting the tubing
14 AssayMAP 96AM Wash Station Maintenance Guide
Note: The wash station is easier to install if you install the platepad
bracket after the wash station is in position.
2Install the wash station on the platepad as follows:
aEnsuring that the inlet and outlet ports are towards the rear of the
deck, place the wash station on the platepad.
bGuide the platepad inner bracket into the wash station’s horizontal
side slot.
cRe- install the remaining platepad bracket. Ensure that you guide the
bracket into the corresponding horizontal slot on the wash station.
Ensure that both brackets remain engaged as you tighten the bracket
screws.
dEnsure that the wash station sits level on the platepad and that both
brackets are holding the wash station securely in place.
Figure 96AM Wash Station secured with platepad brackets
Tubing connections for 96AM Wash Station
Reconnect any of the disconnected tubing. For reference, see the following
figure As the figure shows, the upper pump (A) is for the fill line and the
lower pump (B) is for the empty line.
Note: You specify which pump is for filling and which pump is for emptying in
Bravo Diagnostics.
Figure 96AM Wash Station fill line (A) and empty line (B)
To ensure proper chimney filling and waste emptying characteristics for the
96AM Wash Station, ensure that you use the proper tubing combination for the
Pump Module. See the following figure and table.
A
B
Source Waste

15
1 96AM Wash Station Maintenance Guide
Installing the wash station and connecting the tubing
AssayMAP 96AM Wash Station Maintenance Guide
Figure Tubing configuration
2
5
463 4
345
7
8
8
6
1
444
6
6
Item Component Description
1Source bottle Supplies the fill liquid for the wash station.
2Waste bottle Collects the waste liquid that is pumped from the reservoir.
3Tubing, 8- mm
(5/16- in)
The length of tubing in the lower pump head, which is used for emptying
the wash station. The larger diameter tubing ensures that the fluid is
removed efficiently from the wash station
4Tubing, 6.4- mm
(1/4- in)
The fill- line tubing from the source bottle to the wash station, and
empty- line tubing connections on either side of the 5/16- in diameter
tubing.
Note: The 5/16- in diameter tubing is too large for the quick- disconnect
fittings and the wash station ports. So, you use a union connector (5) to
splice the 5/16- in tubing to the 1/4- in tubing.
5Connector,
union
Joins the two sizes of tubing.
6Quick-
disconnect
fitting
Enables easy removal of the wash station and quick replacement of the
tubing in the pump heads. The quick- disconnect fittings include an
automatic- close valve. Fluid can flow only if a positive connection is
made.
7Inline pump
filter
Removes the particulates that can clog the chimneys in the wash station.
83- way
connector
Enables one tube to branch into two tubes at the input and output ports
on the wash station.

1 96AM Wash Station Maintenance Guide
Testing the 96AM Wash Station
16 AssayMAP 96AM Wash Station Maintenance Guide
Testing the 96AM Wash Station
Before you start
For the teachpoint verification workflow, see “Required resources and
workflow” on page 4.
Starting up the AssayMAP Bravo Platform
To test the performance of the wash station, you run the Startup utility in the
Protein Sample Prep Workbench. During the Startup protocol, carefully observe
the fluid as it flows out of the top of the chimneys in the wash station. If all
the chimneys are flowing evenly, the device is ready for operation.
To start up the system:
1Check the levels of the wash station source and waste carboys, and fill or
empty as required.
2Turn on the AssayMAP Bravo Platform, Pump Module, and the Peltier
Thermal Station Controller, if included.
3Start the Protein Sample Preparation Workbench, and open the Utility Library.
4Open the System Startup/Shutdown utility .
5Click Run Startup to initialize the AssayMAP Bravo Platform and accessories.
6During the Startup protocol, verify that all the wash station chimneys have
liquid flowing through them.
Troubleshooting problems with the wash station
Locate your problem in the following table and try the solution. If the problem
persists after you try the solutions, contact Automation Solutions Technical
Support.
CAUTION Any time that you uninstall and reinstall the wash station, the wash station
teachpoint can shift. To prevent a potential crash, verify the wash station teachpoint in all the
profiles any time you reinstall the wash station.
WARNING When you initialize the Bravo Platform, the head and tie bar can move.
To prevent injury, keep clear of the device while it is in motion.

17
1 96AM Wash Station Maintenance Guide
Testing the 96AM Wash Station
AssayMAP 96AM Wash Station Maintenance Guide
Problem Potential cause Solution
Liquid flow is
constricted or
insufficient in all the
chimneys of the
wash station.
The tubing is kinked or
blocked, or the pump
head clamp is too tight
for the tube size.
Ensure the tubing is not kinked or blocked.
Replace the tubing as necessary.
If necessary adjust the pump head clamp, see the
Agilent Pump Module User Guide.
The inline filter is
clogged.
Replace the inline filter.
See “Tubing connections for 96AM Wash Station”
on page 14.
The tubing diameter of
the fill line is too small.
Replace the tubing on the fill line using larger
diameter tubing.
See “Tubing connections for 96AM Wash Station”
on page 14.
Liquid is not flowing
through a subset of
the chimneys in the
wash station.
The chimneys are
blocked or damaged.
To resolve the problem chimneys:
1Use a plastic Pasteur pipette to quickly
aspirate liquid through the chimneys.
2Run the Pump Reagent task in Bravo
Diagnostics to pump liquid through the
chimneys at a maximum rate to dislodge any
clogs.
3If the problem persists, replace the problematic
chimneys. See “Replacing damaged chimneys”
on page 11
Waste is
accumulating in the
wash station.
The tubing is kinked or
blocked, or the pump
head clamp is too tight
for the tube size.
Ensure the tubing is not kinked or blocked.
Replace the tubing as necessary.
If necessary adjust the pump head clamp, see the
Agilent Pump Module User Guide.
The tubing diameter is
too small for the
required flow.
Replace the tubing on the empty line, using larger
diameter tubing for the section that routes through
the pump head (active section).
See “Tubing connections for 96AM Wash Station”
on page 14.
Tubing creeps
through the pump
head instead of
being secured in
place.
The pump head clamp is
too loose for the tube
size.
Adjust the tubing clamp for the tube size. See the
Agilent Pump Module User Guide.
The tubing is
crushed or damaged
in the pump head.
The pump head clamp is
too tight for the tube
size.
Adjust the tubing clamp for the tube size. See the
Agilent Pump Module User Guide.

Agilent Technologies
Maintenance Guide
G5542-90004
Revision B, February 2018