Agilent Technologies AssayMAP 96AM Operating manual


AssayMAP 96AM
Wash Station
Maintenance Guide

Notices
© Agilent Technologies, Inc. 2018
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United States and international copyright
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Installation Guide Part Number
G5542-90004
Edition
Revision B, February 2018
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fully understood and met.

1
AssayMAP 96AM Wash Station
Maintenance Guide

96AM Wash Station Maintenance
Guide
This guide contains the following topics:
•“About this guide” on page 2
•“About the 96AM Wash Station” on page 4
•“Required resources and workflow” on page 4
•“Determining if the chimneys are in good condition” on page 6
•“Emptying the 96AM Wash Station” on page 6
•“Uninstalling the 96AM Wash Station” on page 10
•“Replacing damaged chimneys” on page 11
•“Installing the wash station and connecting the tubing” on
page 13
•“Testing the 96AM Wash Station” on page 16

1 96AM Wash Station Maintenance Guide
About this guide
2AssayMAP 96AM Wash Station Maintenance Guide
About this guide
Who should read this guide
The instructions in this guide assume that you have the following:
•An understanding of the AssayMAP Bravo Platform
•Familiarity with the Protein Sample Prep Workbench
•Experience in working on mechanical equipment
What this guide covers
This guide describes how to inspect and replace chimneys in the 96AM Wash
Station.
Figure 96AM Wash Station
The 96AM Wash Station is a component of the AssayMAP autofilling station, as
the following figure shows.
Figure AssayMAP autofilling station, including 96AM Wash Station, Pump Module, tubing, and carboys
Source Waste

3
1 96AM Wash Station Maintenance Guide
About this guide
AssayMAP 96AM Wash Station Maintenance Guide
Related user guides and where to find them
Accessing the user documentation
You can access the user documentation in the Protein Sample Prep Workbench
as follows:
•Application user guides. In the Protein Sample Prep Workbench, click Utility
Library, locate the utility of interest, and then click Instructions.
•Utility instructions. In the Protein Sample Prep Workbench, click Literature
Library, locate the topic of interest, and then click Open.
Alternatively, you can access the user documentation in the VWorks Knowledge
Base using either of the following methods:
•From within VWorks software, select Help > Knowledge Base or press F1.
•From the Windows desktop, do one of the following:
–Windows 10. Select Start > All Apps > Agilent Technologies > VWorks
Knowledge Base.
–Windows 7. Select Start > All Programs > Agilent Technologies > VVWorks >
User Guides > VWorks Knowledge Base.
•Go to www.agilent.com/chem/askb.
For information on… See…
Potential safety hazards and how to
avoid them
Bravo Platform Safety and
Installation Guide
Using the Protein Sample Prep
Workbench
Literature Library in the Protein
Sample Prep Workbench
Setting up, operating, maintaining, and
troubleshooting the Bravo Platform
Bravo Platform User Guide
Setting teachpoints for the AssayMAP
Bravo Platform
Bravo Platform User Guide
Setting up the Pump Module and the
autofilling reservoirs and wash stations
Pump Module User Guide
Creating and editing VWorks protocols
and application interfaces
VWorks Automation Control User
Guide
Running a syringe test of the Bravo
96AM Head
AssayMAP Syringe Test Utility
User Guide in the Protein Sample
Prep Workbench
Replacing a defective syringe in the
Bravo 96AM Head
AssayMAP Bravo Syringe
Replacement User Guide

1 96AM Wash Station Maintenance Guide
About the 96AM Wash Station
4AssayMAP 96AM Wash Station Maintenance Guide
About the 96AM Wash Station
The 96AM Wash Station has 96 chimneys and is scaled to work with the
syringe probes in the Bravo 96AM Head. The wash station is used for washing
the syringes and washing cartridges or pipette tips that are seated on the
syringe probes.
Figure Chimney detail in the 96AM Wash Station
Fresh wash liquid enters the 96AM Wash Station through two inlet ports (1),
shown in the following figure. The wash liquid flows up through the chimneys,
and the waste overflows from the chimneys and is removed through two outlet
ports (2).
Figure Inlet ports (1) and outlet ports (2) in the 96AM Wash Station
Required resources and workflow
Ensure that you have the following tools and materials:
•Replacement Wash Station Chimneys: large internal diameter, pack 100,
Agilent part number 15586- 002
•Hex driver to uninstall and install 2- mm hex screws
•1 needle- nose pliers to remove the bad chimneys from the 96AM Wash
Station
•Small rubber pad or foam to protect your thumb when seating a new
chimney in the 96AM Wash Station.
CAUTION Ensure that you use only the 96AM Wash Station on the AssayMAP Bravo
Platform. Using a model of the Tip Wash Station other than the 96AM Wash Station can result
in a potential crash with the Bravo 96AM Head.
WARNING The probes of the Bravo 96AM Head are sharp and can scratch you if they
brush across your hand. A probe scratch can expose you to any contaminants remaining on
the probes. Wear gloves and use extreme caution to avoid brushing against the probes.

5
1 96AM Wash Station Maintenance Guide
Required resources and workflow
AssayMAP 96AM Wash Station Maintenance Guide
Perform the following steps in the order given.
Step For this task... See...
1Inspect the chimneys for damage and
irregularities.
“Determining if the
chimneys are in good
condition” on page 6
2Empty the wash station. “Emptying the 96AM Wash
Station” on page 6
3Uninstall the wash station. “Uninstalling the 96AM
Wash Station” on page 10
4Replace the damaged chimneys. “Replacing damaged
chimneys” on page 11
5Install the wash station. “Installing the wash station
and connecting the tubing”
on page 13
6Verify the wash station teachpoint
and adjust it, as necessary:
aInitialize the AssayMAP 1
profile and adjust the
teachpoint for the 96AM Wash
Station.
bUse the AssayMAP Teachpoint
Update protocol to update the
teachpoint in the remaining
profiles in the Protein Sample
Prep Workbench
automatically.
AssayMAP Bravo Platform
Installation Guide (for field
service personnel) or
Bravo Platform User Guide
7Start up the AssayMAP Bravo
Platform and verify that all the
chimneys work consistently.
“Testing the 96AM Wash
Station” on page 16

1 96AM Wash Station Maintenance Guide
Determining if the chimneys are in good condition
6AssayMAP 96AM Wash Station Maintenance Guide
Determining if the chimneys are in good condition
During normal operation of the 96AM Wash Station, the fluid flow out of the
top of each chimney and the shape of the fluid dome at the top of each
chimney must consistent. To ensure consistency in the fluid flow from each
chimney, the chimneys must be:
•Clean and free of any debris.
•Free of deformities or irregularities.
See the good condition chimneys in the following figure. The chimneys
must not be damaged, such as those shown in the figure (possibly from a
head collision).
•Equal in height and installed in the same orientation.
Figure Close up views of 96AM Wash Station chimneys
Chimney irregularities or damaged chimneys can cause large droplets to cling
to the syringe probes, which can introduce variability in the assay.
Emptying the 96AM Wash Station
This topic describes how to use the Pump Reagent task in Bravo Diagnostics to
empty any fluid from the wash station.
Before you start
WARNING The probes of the Bravo 96AM Head are sharp and can scratch you if they
brush across your hand. A probe scratch can expose you to any contaminants remaining on
the probes. Wear gloves and use extreme caution to avoid brushing against the probes.
WARNING When you initialize the Bravo Platform, the head and tie bar can move. To
prevent potential injury, keep clear of the device while it is in motion.
CAUTION To prevent potential equipment damage, ensure that the deck is clear of any
obstacles before initializing the AssayMAP Bravo Platform.

7
1 96AM Wash Station Maintenance Guide
Emptying the 96AM Wash Station
AssayMAP 96AM Wash Station Maintenance Guide
Running the Pump Reagent task to empty the wash station
To empty the wash station using the Pump Reagent task:
1At the carboy that contains the wash solution, remove the lid and lift it so
that the tubing is above the liquid level.
2Ensure that the Protein Sample Prep Workbench is closed, and then start
the VWorks software.
You can use the VWorks desktop icon or the Start menu on the Windows
desktop to start the VWorks software.
3Ensure that Simulation is off.
4In the VWorks window, open the AssayMAP Bravo device file
(AssayMAP Bravo.dev).
To do this, click File > Open. In the Open dialog box, ensure the file type is
Device Files (*.dev), select the file, and then click Open.
The device files are installed in the following default location:
C:\VWorks Workspace\Workbench\Device Files
5Click No when the Would you like to initialize devices now? message appears.
6In the AssayMAP Bravo.dev tab, click Device diagnostics. The Bravo Diagnostics
dialog opens.
CAUTION Using an incorrect profile can cause equipment damage. Ensure that the profile
is correct for the head type and deck configuration.

1 96AM Wash Station Maintenance Guide
Emptying the 96AM Wash Station
8AssayMAP 96AM Wash Station Maintenance Guide
Figure AssayMAP Bravo.dev tab and Profile setting for AssayMAP 1
7Click the Profiles tab, select the Profile name AssayMAP 1, and then click
Initialize this profile.
8If the There appears to be a plate present error message appears, click Ignore and
Continue to continue the homing process.
9When the Verify that it is safe to home the W-axis message appears, click Retry to
continue homing the w-axis.