Agilent Technologies G1992A User manual

Agilent G1992A
Nanospray Ion Source
User Guide

Nanospray Ion Source User Guide
Notices
© Agilent Technologies, Inc. 2012
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Manual Part Number
G1988-90000
Edition
November, 2012
Printed in USA
Agilent Technologies, Inc.
5301 Stevens Creek Blvd.
Santa Clara, CA 95051 USA
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Nanospray Ion Source User Guide 3
In This Guide…
This guide contains information to install and set up the
Nanospray Ion Source.
1 Installation
In this chapter you install the source and prepare the system for
analysis.
2MassReferenceSolution
This chapter describes how to use the Mass Reference Solution
to calibrate the Nanospray Ion Source.
3Safety
This chapter contains safety information.
4 Reference
This chapter contains parts lists and other reference
information.

4 Nanospray Ion Source User Guide

Nanospray Ion Source User Guide 5
Content
1 Installation 7
Upgrade Preparation 8
To remove the ESI or Agilent Jet Stream source 8
To upgrade the gas distributor 9
To upgrade the stainless steel end plate 12
To assemble and install the new spray shield and gas diverter assembly 13
Installation 16
To install the source 17
To set the position of the Counter Electrode 18
To set up MassHunter program for the Nanospray Ion Source 21
To install the power cord and camera 22
To assemble the Infusion Sample Delivery System 25
To infuse solution through the Infusion Sample Delivery System 26
To assemble the Extension Tube column support assembly for LC analysis 27
To install the Needle Sleeve Assembly into the Nanospray Ion Source 32
To install the Nanospray Slide Assembly for inline analysis 36
To adjust the LED camera light 41
2MassReferenceSolution43
To prepare the Low Mass IRM stock solution 45
To prepare the IRM working solution 46
To apply the IRM solutions 47
To enable reference mass correction 50
To clean the wick and wick cover 52
3Safety53
Safety Issues 54
Safety Symbols 58

6 Nanospray Ion Source User Guide
Contents
4 Reference 59
Parts List 60
Component Alignment 67
Hole Layout 68

7
Agilent G1992A Nanospray Ion Source
User Guide
1
Installation
Upgrade Preparation 8
To remove the ESI or Agilent Jet Stream source 8
To upgrade the gas distributor 9
To upgrade the stainless steel end plate 12
To assemble and install the new spray shield and gas diverter
assembly 13
Installation 16
To install the source 17
To set the position of the Counter Electrode 18
To set up MassHunter program for the Nanospray Ion Source 21
To install the power cord and camera 22
To assemble the Infusion Sample Delivery System 25
To infuse solution through the Infusion Sample Delivery System 26
To assemble the Extension Tube column support assembly for LC
analysis 27
To install the Needle Sleeve Assembly into the Nanospray Ion
Source 32
To install the Nanospray Slide Assembly for inline analysis 36
To adjust the LED camera light 41

1 Installation
8 Nanospray Ion Source User Guide
Upgrade Preparation
The installation kit contains several upgrade kits for older instruments:
•gas distributor (p/n G1964-20303)
•spray shield kit (p/n G1988-60007)
If you have an installed instrument, inspect the instrument to determine
which of these kits need to be installed. These parts are already installed on
new instruments.
You also need to adjust the position of the Counter Electrode. A 1.5 mm hex
wrench, and a 7 mm spacing tool are provided for setting the distance of the
Counter Electrode from the spray shield.
To remove the ESI or Agilent Jet Stream source
1Put the instrument into standby mode and lower the source temperatures
to start cooling down the source.
2When the source is cool, turn off the nebulizer, drying gas, and sheath gas
flow (if using the Jet Stream source).
3Remove the existing source, install the protective cover and store in a safe
place.
4Remove the spray shield and store with the source.
NOTE
Autotune is not supported with the Agilent G1992A Nanospray Ion Source. Before
changing the source, do all tuning with the ESI or Agilent Jet Stream source. Calibration
can still be done with the Nanospray Ion Source.

Installation 1
Nanospray Ion Source User Guide 9
To upgrade the gas distributor
Newer model gas distributors are required to support the improved resistive
or dielectric capillary. If your gas distributor looks like the one in Figure 1,
replace it with the new one.
1Remove the chamber and capillary high voltage cable leads from the T-nut
connectors.
Figure 1 Remove the four T20 Torx screws from the metal ring.
2Remove the four T-20 torx screws used to secure the metal ring that holds
the PTFE spray shield support block in place.
3Remove the PTFE spray shield support block to access the vespel gas
distributor. Remove and discard the old gas distributor.

1 Installation
10 Nanospray Ion Source User Guide
Figure 2 Remove the old gas distributor
4Install the new gas distributor (p/n G1964-20303) with the solid side facing
the PTFE wall. Position it so that it is flush with the PTFE plate.
Figure 3 G1964-20303 Gas Distributor