Agilent Technologies G1992A User manual

Agilent G1992A
Nanospray Ion Source
User Guide

Nanospray Ion Source User Guide
Notices
© Agilent Technologies, Inc. 2012
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Manual Part Number
G1988-90000
Edition
November, 2012
Printed in USA
Agilent Technologies, Inc.
5301 Stevens Creek Blvd.
Santa Clara, CA 95051 USA
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Nanospray Ion Source User Guide 3
In This Guide…
This guide contains information to install and set up the
Nanospray Ion Source.
1 Installation
In this chapter you install the source and prepare the system for
analysis.
2MassReferenceSolution
This chapter describes how to use the Mass Reference Solution
to calibrate the Nanospray Ion Source.
3Safety
This chapter contains safety information.
4 Reference
This chapter contains parts lists and other reference
information.

4 Nanospray Ion Source User Guide

Nanospray Ion Source User Guide 5
Content
1 Installation 7
Upgrade Preparation 8
To remove the ESI or Agilent Jet Stream source 8
To upgrade the gas distributor 9
To upgrade the stainless steel end plate 12
To assemble and install the new spray shield and gas diverter assembly 13
Installation 16
To install the source 17
To set the position of the Counter Electrode 18
To set up MassHunter program for the Nanospray Ion Source 21
To install the power cord and camera 22
To assemble the Infusion Sample Delivery System 25
To infuse solution through the Infusion Sample Delivery System 26
To assemble the Extension Tube column support assembly for LC analysis 27
To install the Needle Sleeve Assembly into the Nanospray Ion Source 32
To install the Nanospray Slide Assembly for inline analysis 36
To adjust the LED camera light 41
2MassReferenceSolution43
To prepare the Low Mass IRM stock solution 45
To prepare the IRM working solution 46
To apply the IRM solutions 47
To enable reference mass correction 50
To clean the wick and wick cover 52
3Safety53
Safety Issues 54
Safety Symbols 58

6 Nanospray Ion Source User Guide
Contents
4 Reference 59
Parts List 60
Component Alignment 67
Hole Layout 68

7
Agilent G1992A Nanospray Ion Source
User Guide
1
Installation
Upgrade Preparation 8
To remove the ESI or Agilent Jet Stream source 8
To upgrade the gas distributor 9
To upgrade the stainless steel end plate 12
To assemble and install the new spray shield and gas diverter
assembly 13
Installation 16
To install the source 17
To set the position of the Counter Electrode 18
To set up MassHunter program for the Nanospray Ion Source 21
To install the power cord and camera 22
To assemble the Infusion Sample Delivery System 25
To infuse solution through the Infusion Sample Delivery System 26
To assemble the Extension Tube column support assembly for LC
analysis 27
To install the Needle Sleeve Assembly into the Nanospray Ion
Source 32
To install the Nanospray Slide Assembly for inline analysis 36
To adjust the LED camera light 41

1 Installation
8 Nanospray Ion Source User Guide
Upgrade Preparation
The installation kit contains several upgrade kits for older instruments:
•gas distributor (p/n G1964-20303)
•spray shield kit (p/n G1988-60007)
If you have an installed instrument, inspect the instrument to determine
which of these kits need to be installed. These parts are already installed on
new instruments.
You also need to adjust the position of the Counter Electrode. A 1.5 mm hex
wrench, and a 7 mm spacing tool are provided for setting the distance of the
Counter Electrode from the spray shield.
To remove the ESI or Agilent Jet Stream source
1Put the instrument into standby mode and lower the source temperatures
to start cooling down the source.
2When the source is cool, turn off the nebulizer, drying gas, and sheath gas
flow (if using the Jet Stream source).
3Remove the existing source, install the protective cover and store in a safe
place.
4Remove the spray shield and store with the source.
NOTE
Autotune is not supported with the Agilent G1992A Nanospray Ion Source. Before
changing the source, do all tuning with the ESI or Agilent Jet Stream source. Calibration
can still be done with the Nanospray Ion Source.

Installation 1
Nanospray Ion Source User Guide 9
To upgrade the gas distributor
Newer model gas distributors are required to support the improved resistive
or dielectric capillary. If your gas distributor looks like the one in Figure 1,
replace it with the new one.
1Remove the chamber and capillary high voltage cable leads from the T-nut
connectors.
Figure 1 Remove the four T20 Torx screws from the metal ring.
2Remove the four T-20 torx screws used to secure the metal ring that holds
the PTFE spray shield support block in place.
3Remove the PTFE spray shield support block to access the vespel gas
distributor. Remove and discard the old gas distributor.

1 Installation
10 Nanospray Ion Source User Guide
Figure 2 Remove the old gas distributor
4Install the new gas distributor (p/n G1964-20303) with the solid side facing
the PTFE wall. Position it so that it is flush with the PTFE plate.
Figure 3 G1964-20303 Gas Distributor

Installation 1
Nanospray Ion Source User Guide 11
Figure 4 Install the new gas distributor
5Reinstall the PTFE end plate support block with the stainless steel ring.
Make sure that the new gas distributor is positioned properly before
installing and tightening the screws to avoid damage to the new gas
distributor.

1 Installation
12 Nanospray Ion Source User Guide
To upgrade the stainless steel end plate
If the screws that hold the end plate in position are pan heads, install the
Nanospray Desolvation Upgrade Kit (p/n G1988-60009). This upgrade reduces
any arcing that can occur to the raised head screws.
Before you begin, make sure that you have the parts for Nanospray
Desolvation Upgrade Kit (p/n G1988-60009) listed in Table 7 on page 62.
1Remove the end plate and the attached screws.
2Remove the two T-nut high voltage connectors from the white PTFE block.
3If the existing T-nut connectors are damaged, install the two new T-nut
connectors into the PTFE block.
4Install the new stainless steel end plate. Use the two new gold screws to
secure the end plate. Do not over-tighten these screws!
Figure 5 Flat head gold screws installed on new stainless steel end plate

Installation 1
Nanospray Ion Source User Guide 13
To assemble and install the new spray shield and gas diverter
assembly
Before you begin, make sure that you have the parts for the Spray Shield Kit
(p/n G1988-60007) listed in Table 8 on page 62.
1Slide the Wave washer over the appropriate spray shield:
•Single bore spray shield for single bore capillary in orthogonal
orientation
•Single bore inline spray shield for inline needle orientation
•Multibore vortex spray shield for multibore capillary
2Insert the Radial gas diverter insert into the spray shield. Make sure to
align the pin in the Radial gas diverter with the notch on the End Cap.
Figure 6 Gas diverter pin aligns with the spray shield groove
3Thread the vespel Radial gas diverter vespel into the Winged spray shield
nut. Make sure to thread the Radial gas diverter completely into the Winged
spray shield nut.

1 Installation
14 Nanospray Ion Source User Guide
Figure 7 Radial gas diverter and winged nut
4After all of the parts are assembled, rinse all of these parts with
Isopropanol in a clean glass beaker at least three times.
5After the parts are rinsed, remove the parts from the beaker and place them
on a lint free cloth.
6Insert the Contact spring into the cleaned spray shield assembly.
Figure 8 Contact spring installed onto spray shield assembly

Installation 1
Nanospray Ion Source User Guide 15
7Install the spray shield.
Figure 9 Installed spray shield assembly. (Multibore vortex spray shield shown)
8Verify that the desolvation chamber end plate screws have good clearance
to the end plate.
WARNING
If the screw heads are too close to the winged spray shield nut, arcing can occur.

1 Installation
16 Nanospray Ion Source User Guide
Installation
The source can be configured in three ways:
Orthogonal Orientation Sample enters from
above. The needle is at right angles to the MS
capillary. Adjustment is along Z and Y axes.
Camera is to the left, viewing the spray
orthogonally.
Inline Horizontal Orientation Sample enters
from the front. The needle is parallel with the
capillary. Needle movement is along Z and Y.
The camera is mounted to the left, viewing
the needle movement orthogonally. In this
orientation, the camera plate is located over
the top hole of the chamber. The camera plug
covers the opening.
Inline Sideways Orientation Sample enters
from the front. The needle is parallel to the
capillary. Needle movement is forward and
side-to-side (Z-X). The camera adapter plate
is located over the top hole of the chamber,
and the camera is mounted above, viewing
the needle movement orthogonally. The
camera plug is inserted into the hole vacated
by the camera.

Installation 1
Nanospray Ion Source User Guide 17
To install the source
1Align the hinge pins with their
respective holes on the
desolvation assembly. The source
must be at an angle of less than 90
degrees with respect to the
desolvation chamber.
2Slide the hinge pins down into
their seated position, and rotate
the source into contact with the
desolvation chamber.
3Latch the chambers together.

1 Installation
18 Nanospray Ion Source User Guide
To set the position of the Counter Electrode
For best orthogonal Nanospray Ion Source operation, the Counter Electrode
needs to be about 7 mm away from the end of the winged spray shield cap.
Before you begin, make sure that you have:
•electrode spacing tool (p/n GT430-20358)
•1.5 mm hex wrench
1Loosen the four recessed Torx T10 screws which attach to the spray
chamber body.
2Remove the Nanospray Slide Assembly.
Removal of the Nanospray Slide Assembly creates a larger hole for better
access to the Counter Electrode.
Figure 10 Loosen the 4 screws to remove the Nanospray Slide Assembly.
WARNING
The instrument must be in Standby mode or turned off to do this step. Otherwise, the
spray chamber high voltage electronics will be energized and present a shock
hazard.

Installation 1
Nanospray Ion Source User Guide 19
Figure 11 Nanospray Slide Assembly removed.
3Loosen the set screw for the Counter Electrode using the 1.5 mm hex
wrench
Figure 12 Removing the Nanospray Slide Assembly provides easier access to the set
screw.

1 Installation
20 Nanospray Ion Source User Guide
4Insert the electrode spacing tool (p/n GT430-20358) between the Counter
Electrode and the spray shield.
The tool must touch both the Counter Electrode and the spray shield for
correct positioning. Lightly tighten the set screw to secure the position of
the Counter Electrode.
Figure 13 The electrode spacing tool touches both the spray shield and the Counter
Electrode.
5After tightening the set screw, make a final check with the electrode spacing
tool.
The Counter Electrode must be properly positioned to generate proper
spray.
6Reinstall the Nanospray Slide Assembly.