Agilent Technologies 1260 Infinity User manual

Agilent Technologies
Agilent 1260 Infinity
Isocratic Pump and
Quaternary Pump
User Manual

Agilent 1260 IsoPump/QuatPump User Manual
Notices
© Agilent Technologies, Inc. 2010
No part of this manual may be reproduced
in any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G1310-90012
Edition
06/10
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
This product may be used as a com-
ponent of an in vitro diagnostic sys-
tem if the system is registered with
the appropriate authorities and com-
plies with the relevant regulations.
Otherwise, it is intended only for gen-
eral laboratory use.
Warranty
The material contained in this docu-
ment is provided “as is,” and is sub-
ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a par-
ticular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or perfor-
mance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the sep-
arate agreement shall control.
Technology Licenses
The hardware and/or software described in
this document are furnished under a license
and may be used or copied only in accor-
dance with the terms of such license.
Restricted Rights Legend
If software is for use in the performance of a
U.S. Government prime contract or subcon-
tract, Software is delivered and licensed as
“Commercial computer software” as
defined in DFAR 252.227-7014 (June 1995),
or as a “commercial item” as defined in FAR
2.101(a) or as “Restricted computer soft-
ware” as defined in FAR 52.227-19 (June
1987) or any equivalent agency regulation
or contract clause. Use, duplication or dis-
closure of Software is subject to Agilent
Technologies’ standard commercial license
terms, and non-DOD Departments and
Agencies of the U.S. Government will
receive no greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will receive
no greater than Limited Rights as defined in
FAR 52.227-14 (June 1987) or DFAR
252.227-7015 (b)(2) (November 1995), as
applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully under-
stood and met.

Agilent 1260 IsoPump/QuatPump User Manual 3
Contents
Contents
1 Introduction 7
Introduction to the Pump 8
Overview of the Hydraulic Path 10
Early Maintenance Feetback 17
Instrument Layout 18
Electrical Connections 19
Interfaces 21
Setting the 8-bit Configuration Switch 27
2 Site Requirements and Specifications 35
Site Requirements 36
Physical Specifications 39
Performance Specifications 40
3 Installing the Pump 43
Unpacking the Pump 44
Optimizing the Stack Configuration 47
Installing the Pump 50
Connecting Modules and Control Software 53
Flow Connections of the Pump 56
Priming the System 59
4UsingthePump 65
Hints for Successful Use of the Isocratic Pump 66
Prevent Blocking of Solvent Filters 68
Algae Growth in HPLC Systems 69
5 Optimiziming Performance 71
When to Use a Vacuum Degasser 72
Operational Hints for the Multi Channel Gradient Valve (MCGV) 73
When to use the Seal Wash Option 74
Choosing the Right Pump Seals 76
Optimize the Compressibility Compensation Setting 77

4 Agilent 1260 IsoPump/QuatPump User Manual
Contents
6 Troubleshooting and Diagnostics 79
Overview of the Module’s Indicators and Test Functions 80
Status Indicators 82
User Interfaces 84
Agilent Lab Advisor Software 85
7 Error Information 87
What Are Error Messages 89
General Error Messages 90
Module Error Messages 96
8 Test Functions and Calibration 111
Introduction 112
System Pressure Test 113
Leak Rate Test 114
9 Maintenance 115
Introduction to Maintenance and Repair 116
Warnings and Cautions 117
Overview of Maintenance 119
Cleaning the Module 120
Checking and Cleaning the Solvent Filter 121
Exchanging the Passive Inlet Valve (PIV) 123
Exchanging the Outlet Valve 124
Exchanging the Purge Valve Frit or the Purge Valve 126
Removing the Pump Head Assembly 128
Maintenance of a Pump Head Without Seal Wash Option 130
Seal Wear-in Procedure 134
Maintenance of a Pump Head with Seal Wash Option 135
Reinstalling the Pump Head Assembly 138
Exchanging the Multi-Channel Gradient Valve (MCGV) 140
Exchanging the Optional Interface Board 143
Exchanging the Active Inlet Valve (AIV) or its Cartridge 145
Replacing the Module’s Firmware 147

Agilent 1260 IsoPump/QuatPump User Manual 5
Contents
10 Parts for Maintenance 149
Pump Head Assembly Without Seal Wash 150
Pump Head Assembly with Seal Wash Option (600 bar) 152
Outlet Valve 154
Purge Valve Assembly 155
Active Inlet Valve Assembly 156
Accessory Kit G1311-68755 157
Seal Wash Option Kit 158
Solvent Cabinet 159
Bottle Head Assembly 161
Hydraulic Path 162
11 Identifying Cables 165
Cable Overview 166
Analog Cables 168
Remote Cables 170
BCD Cables 173
CAN/LAN Cables 175
External Contact Cable 176
Agilent Module to PC 177
Agilent 1200 Module to Printer 178
12 Appendix 179
General Safety Information 180
The Waste Electrical and Electronic Equipment Directive 183
Batteries Information 184
Radio Interference 185
Sound Emission 186
Solvent Information 187
Agilent Technologies on Internet 188

6 Agilent 1260 IsoPump/QuatPump User Manual
Contents

7
Agilent 1260 IsoPump/QuatPump User Manual
Agilent Technologies
1
Introduction
Introduction to the Pump 8
Overview of the Hydraulic Path 10
Hydraulic Path 11
How Does the Pump Work? 12
How Does Compressibility Compensation Work? 15
How Does Variable Stroke Volume Work? 16
Early Maintenance Feetback 17
Instrument Layout 18
Electrical Connections 19
Serial Number Information (ALL) 20
Rear View of the Module 20
Interfaces 21
Overview Interfaces 23
Setting the 8-bit Configuration Switch 27
Communication Settings for RS-232C 30
Special Settings 32
This chapter gives an introduction to the module, instrument overview and
internal connectors

8 Agilent 1260 IsoPump/QuatPump User Manual
1Introduction
Introduction to the Pump
Introduction to the Pump
Introduction to the Quaternary Pump
The quaternary pump comprises a solvent cabinet, a vacuum degasser and a
four-channel gradient pump. The four-channel gradient pump comprises a
high-speed proportioning valve and a pump assembly. It provides gradient
generation by low pressure mixing. A solvent cabinet provides enough space
for four one-liter bottles. An active seal wash (optional) is available when the
quaternary pump is used with concentrated buffer solutions.
Figure 1 Overview of the Quaternary Pump
EjbeYg^kZ
Ejbe]ZVY
DjiaZikVakZ
Ejg\ZkVakZ
EVhh^kZ^caZikVakZ
B8<K
;Vc
9VbeZg
EdlZghjeean
Ejbe
bV^cWdVgY
9Z\VhhZg

Agilent 1260 IsoPump/QuatPump User Manual 9
Introduction 1
Introduction to the Pump
Introduction to the Isocratic Pump
The isocratic pump has the same operating principle as the quaternary pump
but has only one solvent channel, that means the composition cannot be
changed during a method because there is no multi-channel gradient valve
(MCGV). The isocratic pump does not include a degasser. An upgrade product
(Isocratic to Quaternary Pump Upgrade Kit (p/n G4207-60000)) is available
for upgrading the isocratic pump to a quaternary pump including a degasser.
Figure 2 Overview of the Isocratic Pump
EjbeYg^kZ
Ejbe]djh^c\
DjiaZikVakZ
Ejg\ZkVakZ
EVhh^kZ^caZikVakZ
EdlZghjeean
BV^cWdVgY
9VbeZg
;Vc

10 Agilent 1260 IsoPump/QuatPump User Manual
1Introduction
Overview of the Hydraulic Path
Overview of the Hydraulic Path
Both the isocratic pump and quaternary pump are based on a two-channel,
dual-plunger in-series design which comprises all essential functions that a
solvent delivery system has to fulfill. Metering of solvent and delivery to the
high-pressure side are performed by one pump assembly which can generate
pressure up to 600 bar.
In the quaternary pump, degassing of the solvents is done in a built-in vacuum
degasser. Solvent compositions are generated on the low-pressure side by a
high-speed proportioning valve (MCGV).
The pump assembly includes a pump head with a passive inlet valve and an
outlet valve. A damping unit is connected between the two piston chambers. A
purge valve including a PTFE frit is fitted at the pump outlet for convenient
priming of the pump head.
An active seal wash (optional) is available for applications using concentrated
buffers as solvents.

Agilent 1260 IsoPump/QuatPump User Manual 11
Introduction 1
Overview of the Hydraulic Path
Hydraulic Path
Figure 3 Hydraulic Path of the Isocratic Pump
9VbeZg
DjiaZikVakZ
Ejg\ZkVakZ
EVhh^kZ^caZikVakZ
Ejbe]ZVY

12 Agilent 1260 IsoPump/QuatPump User Manual
1Introduction
Overview of the Hydraulic Path
Figure 4 Hydraulic Path of the Quaternary Pump
How Does the Pump Work?
In the quaternary pump, the liquid runs from the solvent reservoir through the
degasser to the MCGV and from there to the inlet valve.
For the isocratic pump, the solvent bottle is directly connected to the inlet
valve.
The pump assembly comprises two substantially identical piston/chamber
units. Both piston/chamber units comprise a ball-screw drive and a pump
head with one sapphire piston for reciprocating movement in it.
A servo-controlled variable reluctance motor drives the two ball screw drives
in opposite directions. The gears for the ball-screw drives have different
circumferences (ratio 2:1) allowing the first piston to move at twice the speed
of the second piston. The solvent enters the pump head close to the bottom
IdlVhiZ
;gdbhdakZciWdiiaZh
KVXjjbX]VbWZg9Z\VhhZg
DjiaZi
kVakZ
9VbeZg
IdhVbea^c\jc^iVcYXdajbc
>caZi
kVakZ

Agilent 1260 IsoPump/QuatPump User Manual 13
Introduction 1
Overview of the Hydraulic Path
limit and leaves it at its top. The outer diameter of the piston is smaller than
the inner diameter of the pump head chamber allowing the solvent to fill the
gap inbetween. The first piston has a stroke volume in the range of 20 – 100 µL
depending on the flow rate. The microprocessor controls all flow rates in a
range of 1 µL/min – 10 mL/min. The inlet of the first pumping unit is
connected to the passive inlet valve.
The outlet of the first piston/chamber unit is connected through the outlet
valve and the damping unit to the inlet of the second piston/chamber unit. The
outlet of the purge valve assembly is then connected to the following
chromatographic system.
Figure 5 Principle of the Pump
>caZikVakZ
HZVa
7VaahXgZlYg^kZ
Bdidgl^i]ZcXdYZg
<ZVg
IdlVhiZ
Ejg\ZkVakZ
IdXdajbc
9VbeZg
DjiaZi
kVakZ
EjbeX]VbWZg& EjbeX]VbWZg'
E^hidc&
E^hidc'
;gdbhdakZciWdiiaZ$
YZ\VhhZg

14 Agilent 1260 IsoPump/QuatPump User Manual
1Introduction
Overview of the Hydraulic Path
When turned on, the pump runs through an initialization procedure to
determine the upper dead position of the first piston. The first piston moves
slowly upwards into the mechanical stop of the pump chamber and from there
it moves back for a defined distance. The controller stores this piston position
in memory. After this initialization the pump starts operation with the set
parameters. The passive inlet valve opens and the down-moving piston draws
solvent into the first pump chamber. At the same time the second piston
moves upwards delivering to the system. After a controller-defined stroke
length that depends on the flow rate the drive motor is stopped and the
passive inlet valve closes. The motor direction is reversed and moves the first
piston up until it reaches the stored upper limit and at the same time the
second piston moves downwards. Then the sequence starts again moving the
pistons up and down between the two limits. During the up movement of the
first piston the solvent in the pump chamber is pressed through the outlet
valve into the second pump chamber. The second piston draws in half of the
volume displaced by the first piston and the remaining half volume is directly
delivered to the system. During the drawing stroke of the first piston, the
second piston delivers the drawn volume to the system.
Quaternary pump: For solvent compositions from the solvent bottles A, B, C, D
the controller divides the length of the intake stroke in certain fractions in
which the gradient valve connects the specified solvent channel to the pump
input.
Table 1 Isocratic pump details
Dead volume 800 – 1100 µL, depending on back pressure
Materials in contact with mobile phase
Pump head SST, gold, sapphire, ceramic
Active inlet valve SST, gold, sapphire, ruby, ceramic, PTFE
Outlet valve SST, gold, sapphire, ruby
Adapter SST, gold
Purge valve SST, gold, PTFE, ceramic, PEEK
Degasser chamber TFE/PDD Copolymer, FEP, PEEK, PPS

Agilent 1260 IsoPump/QuatPump User Manual 15
Introduction 1
Overview of the Hydraulic Path
For specifications, of the isocratic pump, see Table 15 on page 40. For
specifications of the quaternary pump, see Table 16 on page 41.
How Does Compressibility Compensation Work?
The compressibility of the solvents in use will affect retention-time stability
when the back pressure in the system changes (for example, ageing of
column). In order to minimize this effect, the pump provides a compressibility
compensation feature which optimizes the flow stability according to the
solvent type. The compressibility compensation is set to a default value and
can be changed through the user interface.
Without a compressibility compensation the following will happen during a
stroke of the first piston. The pressure in the piston chamber increases and
the volume in the chamber will be compressed depending on back pressure
and solvent type. The volume displaced into the system will be reduced by the
compressed volume.
Table 2 Quaternary Pump Details
Delay volume 800 – 1100 µL, dependent on back pressure
Materials in contact with mobile phase
MCGV PTFE
Pump head SST, gold, sapphire, ceramic
Active inlet valve SST, gold, sapphire, ruby, ceramic, PTFE
Outlet valve SST, gold, sapphire, ruby
Adapter SST, gold
Purge valve SST, gold, PTFE, ceramic, PEEK
Damping unit Gold, SST
Degasser chamber TFE/PDD Copolymer, FEP, PEEK, PPS

16 Agilent 1260 IsoPump/QuatPump User Manual
1Introduction
Overview of the Hydraulic Path
With a compressibility value set the processor calculates a compensation
volume, that depends on the back pressure of the system and the selected
compressibility. This compensation volume will be added to the normal stroke
volume and compensates the previously described loss of volume during the
delivery stroke of the first piston.
How Does Variable Stroke Volume Work?
Due to the compression of the pump-chamber volume each piston stroke of the
pump will generate a small pressure pulsation, influencing the flow stability of
the pump. The amplitude of the pressure pulsation depends mainly on the
stroke volume and the compressibility compensation for the solvent in use.
Small stroke volumes generate pressure pulsations of smaller amplitude than
higher stroke volumes at the same flow rate. In addition, the frequency of the
pressure pulsations is higher. This decreases the influence of flow pulsations
on quantitative results.
In gradient mode smaller stroke volumes result in a lower flow ripple improve
composition ripple.
The module uses a processor-controlled spindle system for driving its pistons.
The normal stroke volume is optimized for the selected flow rate. Small flow
rates use a small stroke volume while higher flow rates use a higher stroke
volume.
By default, the stroke volume for the pump is set to AUTO mode. This means
that the stroke is optimized for the flow rate in use. A change to larger stroke
volumes is possible but not recommended.

Agilent 1260 IsoPump/QuatPump User Manual 17
Introduction 1
Early Maintenance Feetback
Early Maintenance Feetback
Maintenance requires the exchange of components which are subject to wear
or stress. Ideally, the frequency at which components are exchanged should be
based on the intensity of usage of the module and the analytical conditions,
and not on a predefined time interval. The early maintenance feedback (EMF)
feature monitors the usage of specific components in the instrument, and
provides feedback when the user-selectable limits have been exceeded. The
visual feedback in the user interface provides an indication that maintenance
procedures should be scheduled.
EMF Counters
EMF counters increment with use and can be assigned a maximum limit which
provides visual feedback in the user interface when the limit is exceeded.
Some counters can be reset to zero after the required maintenance procedure.
Using the EMF Counters
The user-settable EMF limits for the EMF Counters enable the early maintenance
feedback to be adapted to specific user requirements. The useful maintenance
cycle is dependent on the requirements for use. Therefore, the definition of the
maximum limits need to be determined based on the specific operating
conditions of the instrument.
Setting the EMF Limits
The setting of the EMF limits must be optimized over one or two maintenance
cycles. Initially the default EMF limits should be set. When instrument
performance indicates maintenance is necessary, take note of the values
displayed by the EMF counters. Enter these values (or values slightly less than
the displayed values) as EMF limits, and then reset the EMF counters to zero.
The next time the EMF counters exceed the new EMF limits, the EMF flag will be
displayed, providing a reminder that maintenance needs to be scheduled.

18 Agilent 1260 IsoPump/QuatPump User Manual
1Introduction
Instrument Layout
Instrument Layout
The industrial design of the module incorporates several innovative features.
It uses Agilent’s E-PAC concept for the packaging of electronics and
mechanical assemblies. This concept is based upon the use of expanded
polypropylene (EPP) layers of foam plastic spacers in which the mechanical
and electronic boards components of the module are placed. This pack is then
housed in a metal inner cabinet which is enclosed by a plastic external
cabinet. The advantages of this packaging technology are:
•virtual elimination of fixing screws, bolts or ties, reducing the number of
components and increasing the speed of assembly/disassembly,
•the plastic layers have air channels molded into them so that cooling air can
be guided exactly to the required locations,
•the plastic layers help cushion the electronic and mechanical parts from
physical shock, and
•the metal inner cabinet shields the internal electronics from
electromagnetic interference and also helps to reduce or eliminate radio
frequency emissions from the instrument itself.

Agilent 1260 IsoPump/QuatPump User Manual 19
Introduction 1
Electrical Connections
Electrical Connections
•The CAN bus is a serial bus with high speed data transfer. The two
connectors for the CAN bus are used for internal module data transfer and
synchronization.
•One analog output provides signals for integrators or data handling
systems.
•The interface board slot is used for external contacts and BCD bottle
number output or LAN connections.
•The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
start, stop, common shut down, prepare, and so on.
•With the appropriate software, the RS-232C connector may be used to
control the module from a computer through a RS-232C connection. This
connector is activated and can be configured with the configuration switch.
•The power input socket accepts a line voltage of 100 – 240 VAC ± 10 % with a
line frequency of 50 or 60 Hz. Maximum power consumption varies by
module. There is no voltage selector on your module because the power
supply has wide-ranging capability. There are no externally accessible
fuses, because automatic electronic fuses are implemented in the power
supply.
NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper
functionality and compliance with safety or EMC regulations.

20 Agilent 1260 IsoPump/QuatPump User Manual
1Introduction
Electrical Connections
Serial Number Information (ALL)
The serial number information on the instrument labels provide the following
information:
Rear View of the Module
Figure 6 Rear view of the pump
CCXZZ00000 Format
CC Country of manufacturing (DE Germany)
X Alphabetic character A-Z (used by manufacturing)
ZZ Alpha-numeric code 0-9, A-Z, where each combination
unambiguously denotes a module (there can be more than one
code for the same module)
00000 Serial number