Agilent Technologies 1260 Infinity User manual

Agilent Technologies
Agilent 1260 Infinity
Isocratic Pump and
Quaternary Pump
User Manual

Agilent 1260 IsoPump/QuatPump User Manual
Notices
© Agilent Technologies, Inc. 2010
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Manual Part Number
G1310-90012
Edition
06/10
Printed in Germany
Agilent Technologies
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stood and met.

Agilent 1260 IsoPump/QuatPump User Manual 3
Contents
Contents
1 Introduction 7
Introduction to the Pump 8
Overview of the Hydraulic Path 10
Early Maintenance Feetback 17
Instrument Layout 18
Electrical Connections 19
Interfaces 21
Setting the 8-bit Configuration Switch 27
2 Site Requirements and Specifications 35
Site Requirements 36
Physical Specifications 39
Performance Specifications 40
3 Installing the Pump 43
Unpacking the Pump 44
Optimizing the Stack Configuration 47
Installing the Pump 50
Connecting Modules and Control Software 53
Flow Connections of the Pump 56
Priming the System 59
4UsingthePump 65
Hints for Successful Use of the Isocratic Pump 66
Prevent Blocking of Solvent Filters 68
Algae Growth in HPLC Systems 69
5 Optimiziming Performance 71
When to Use a Vacuum Degasser 72
Operational Hints for the Multi Channel Gradient Valve (MCGV) 73
When to use the Seal Wash Option 74
Choosing the Right Pump Seals 76
Optimize the Compressibility Compensation Setting 77

4 Agilent 1260 IsoPump/QuatPump User Manual
Contents
6 Troubleshooting and Diagnostics 79
Overview of the Module’s Indicators and Test Functions 80
Status Indicators 82
User Interfaces 84
Agilent Lab Advisor Software 85
7 Error Information 87
What Are Error Messages 89
General Error Messages 90
Module Error Messages 96
8 Test Functions and Calibration 111
Introduction 112
System Pressure Test 113
Leak Rate Test 114
9 Maintenance 115
Introduction to Maintenance and Repair 116
Warnings and Cautions 117
Overview of Maintenance 119
Cleaning the Module 120
Checking and Cleaning the Solvent Filter 121
Exchanging the Passive Inlet Valve (PIV) 123
Exchanging the Outlet Valve 124
Exchanging the Purge Valve Frit or the Purge Valve 126
Removing the Pump Head Assembly 128
Maintenance of a Pump Head Without Seal Wash Option 130
Seal Wear-in Procedure 134
Maintenance of a Pump Head with Seal Wash Option 135
Reinstalling the Pump Head Assembly 138
Exchanging the Multi-Channel Gradient Valve (MCGV) 140
Exchanging the Optional Interface Board 143
Exchanging the Active Inlet Valve (AIV) or its Cartridge 145
Replacing the Module’s Firmware 147

Agilent 1260 IsoPump/QuatPump User Manual 5
Contents
10 Parts for Maintenance 149
Pump Head Assembly Without Seal Wash 150
Pump Head Assembly with Seal Wash Option (600 bar) 152
Outlet Valve 154
Purge Valve Assembly 155
Active Inlet Valve Assembly 156
Accessory Kit G1311-68755 157
Seal Wash Option Kit 158
Solvent Cabinet 159
Bottle Head Assembly 161
Hydraulic Path 162
11 Identifying Cables 165
Cable Overview 166
Analog Cables 168
Remote Cables 170
BCD Cables 173
CAN/LAN Cables 175
External Contact Cable 176
Agilent Module to PC 177
Agilent 1200 Module to Printer 178
12 Appendix 179
General Safety Information 180
The Waste Electrical and Electronic Equipment Directive 183
Batteries Information 184
Radio Interference 185
Sound Emission 186
Solvent Information 187
Agilent Technologies on Internet 188

6 Agilent 1260 IsoPump/QuatPump User Manual
Contents

7
Agilent 1260 IsoPump/QuatPump User Manual
Agilent Technologies
1
Introduction
Introduction to the Pump 8
Overview of the Hydraulic Path 10
Hydraulic Path 11
How Does the Pump Work? 12
How Does Compressibility Compensation Work? 15
How Does Variable Stroke Volume Work? 16
Early Maintenance Feetback 17
Instrument Layout 18
Electrical Connections 19
Serial Number Information (ALL) 20
Rear View of the Module 20
Interfaces 21
Overview Interfaces 23
Setting the 8-bit Configuration Switch 27
Communication Settings for RS-232C 30
Special Settings 32
This chapter gives an introduction to the module, instrument overview and
internal connectors

8 Agilent 1260 IsoPump/QuatPump User Manual
1Introduction
Introduction to the Pump
Introduction to the Pump
Introduction to the Quaternary Pump
The quaternary pump comprises a solvent cabinet, a vacuum degasser and a
four-channel gradient pump. The four-channel gradient pump comprises a
high-speed proportioning valve and a pump assembly. It provides gradient
generation by low pressure mixing. A solvent cabinet provides enough space
for four one-liter bottles. An active seal wash (optional) is available when the
quaternary pump is used with concentrated buffer solutions.
Figure 1 Overview of the Quaternary Pump
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Agilent 1260 IsoPump/QuatPump User Manual 9
Introduction 1
Introduction to the Pump
Introduction to the Isocratic Pump
The isocratic pump has the same operating principle as the quaternary pump
but has only one solvent channel, that means the composition cannot be
changed during a method because there is no multi-channel gradient valve
(MCGV). The isocratic pump does not include a degasser. An upgrade product
(Isocratic to Quaternary Pump Upgrade Kit (p/n G4207-60000)) is available
for upgrading the isocratic pump to a quaternary pump including a degasser.
Figure 2 Overview of the Isocratic Pump
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10 Agilent 1260 IsoPump/QuatPump User Manual
1Introduction
Overview of the Hydraulic Path
Overview of the Hydraulic Path
Both the isocratic pump and quaternary pump are based on a two-channel,
dual-plunger in-series design which comprises all essential functions that a
solvent delivery system has to fulfill. Metering of solvent and delivery to the
high-pressure side are performed by one pump assembly which can generate
pressure up to 600 bar.
In the quaternary pump, degassing of the solvents is done in a built-in vacuum
degasser. Solvent compositions are generated on the low-pressure side by a
high-speed proportioning valve (MCGV).
The pump assembly includes a pump head with a passive inlet valve and an
outlet valve. A damping unit is connected between the two piston chambers. A
purge valve including a PTFE frit is fitted at the pump outlet for convenient
priming of the pump head.
An active seal wash (optional) is available for applications using concentrated
buffers as solvents.