Agilent Technologies Cary 50 User manual

Cary 50
Spectrophotometer
Hardware Manual

2Cary 50 Spectrophotometer Hardware Manual
Notices
© Agilent Technologies, Inc. 1998,
1999, 2002, 2004, 2006, 2010 and 2011
No part of this manual may be
reproduced in any form or by any
means (including electronic storage
and retrieval or translation into a
foreign language) without prior
agreement and written consent from
Agilent Technologies, Inc. as governed
by United States and international
copyright laws.
Manual Part Number
8510159600
Edition
Ninth edition, March 2011
Printed in Australia
Agilent Technologies, Inc.
Warranty
The material contained in this
document is provided “as is,” and is
subject to being changed, without
notice, in future editions. Further, to
the maximum extent permitted by
applicable law, Agilent disclaims all
warranties, either express or implied,
with regard to this manual and any
information contained herein,
including but not limited to the
implied warranties of merchantability
and fitness for a particular purpose.
Agilent shall not be liable for errors
or for incidental or consequential
damages in connection with the
furnishing, use, or performance of
this document or of any information
contained herein. Should Agilent and
the user have a separate written
agreement with warranty terms
covering the material in this
document that conflict with these
terms, the warranty terms in the
separate agreement shall control.
Technology Licenses
The hardware and/or software
described in this document are
furnished under a license and may be
used or copied only in accordance
with the terms of such license.
Restricted Rights Legend
If software is for use in the
performance of a U.S. Government
prime contract or subcontract,
Software is delivered and licensed as
“Commercial computer software” as
defined in DFAR 252.227-7014 (June
1995), or as a “commercial item” as
defined in FAR 2.101(a) or as
“Restricted computer software” as
defined in FAR 52.227-19 (June 1987)
or any equivalent agency regulation or
contract clause. Use, duplication or
disclosure of Software is subject to
Agilent Technologies‟ standard
commercial license terms, and non-
DOD Departments and Agencies of the
U.S. Government will receive no
greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will
receive no greater than Limited Rights
as defined in FAR 52.227-14 (June
1987) or DFAR 252.227-7015 (b)(2)
(November 1995), as applicable in any
technical data.
Safety Notices
A CAUTION notice denotes a hazard.
It calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION notice
until the indicated conditions are fully
understood and met.
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or the
like that, if not correctly performed or
adhered to, could result in personal
injury or death. Do not proceed
beyond a WARNING notice until the
indicated conditions are fully
understood and met.
WARNING
CAUTION

Contents
Cary 50 Spectrophotometer Hardware Manual 3
Contents
1. Safety Practices and Hazards 5
Lamp module 6
Electrical hazards 6
Modules, covers and panels 6
Other precautions 7
Warning and caution messages 7
Information symbols 8
Color coding 9
CE compliance 10
2. Introduction 11
Installation requirements11
Documentation 11
Conventions 12
Specifications 12
Environmental 13
Weights and dimensions13
Computer power supply 13
Connections 14
3. Installation 15
Cell holder 16
Alignment 18
Other sample holders 18

Contents
4Cary 50 Spectrophotometer Hardware Manual
Uninstalling the Cary WinUV software 19
Uninstalling the Cary 50 driver 19
4. Maintenance 21
Cleaning 22
Lamp module 22
Replacing 22
Aligning the source mirror 25
5. Troubleshooting 27
Windows XP service or driver failure 27
Access denied during installation28
FCary service failed to start 29
No green light indicating that the Cary 50 is powered 30
Start button replaced with Connect button 30
Hard disk power supply connection 32
Absorbance is 10 and fluctuates wildly during a scan 32
Instrument performance testing 33
Wavelength calibration 35
6. Spare Parts 37

Safety Practices and Hazards
Cary 50 Spectrophotometer Hardware Manual 5
1. Safety Practices and Hazards
Lamp module 6
Electrical hazards 6
Modules, covers and panels 6
Other precautions 7
Warning and caution messages 7
Information symbols 8
Color coding 9
CE compliance 10
Your Agilent Cary 50 instrument and accessories have been carefully
designed so that when used properly, you have an accurate, fast,
flexible and safe analytical system.
If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be
impaired.
Information about safety practices appears throughout the
documentation (both hard copy and online) provided with your
instrument and accessories to help you safely operate the instrument
and accessories. Before using the instrument or accessories, you
must thoroughly read these safety practices. ALWAYS operate the
instrument and accessories in accordance with these safety practices.

Safety Practices and Hazards
6Cary 50 Spectrophotometer Hardware Manual
Lamp module
The lamp is enclosed in a self-contained module. This module
contains components operating at high voltages. To avoid electric
shock, NEVER disassemble the module.
When operating, the lamp module emits high-intensity light, which
can cause serious damage to eyes. To avoid eye damage, never
operate the lamp outside the instrument.
Electrical hazards
The Cary 50 spectrophotometer is powered by the computer
controlling the instrument. Safe operation of the instrument depends
on the integrity of the switching power supply of the computer. Your
computer must comply with IEC 60950.
Modules, covers and panels
The ONLY modules of the Cary 50 spectrophotometer that operators
and other unauthorized personnel are permitted to access are the
lamp module (on the underside of the instrument) and the sample
compartment. White circles indicate the screws you need to undo to
remove the lamp module. ALWAYS switch off the computer before
changing a lamp.
The only panel you are permitted to remove is the snap-out panel
covering the lamp mirror adjustment screws on the front of the
instrument.
Any other panels or covers that are retained by screws on the
spectrophotometer and accessories may be opened ONLY by Agilent-
trained, Agilent-qualified, or Agilent-approved customer support
representatives. Consult the manuals or product labels supplied with
your computer, monitor and printer to determine which parts are
operator-accessible.
NOTE
The safety classification is given as Safety Class III (EN 61010-1).

Safety Practices and Hazards
Cary 50 Spectrophotometer Hardware Manual 7
Other precautions
Do not block any ventilation grilles present on the computer. Consult
the manuals supplied with your computer, monitor and printer for
their specific ventilation requirements.
Use of the Cary 50 system and accessories may involve materials,
solvents and solutions that are flammable, corrosive, toxic or
otherwise hazardous.
Careless, improper, or unskilled use of such materials, solvents and
solutions can create explosion hazards, fire hazards, toxicity and
other hazards which can result in death, serious personal injury, and
damage to equipment and property.
ALWAYS ensure that laboratory safety practices governing the use,
handling and disposal of such materials are strictly observed. These
safety practices should include the wearing of appropriate safety
clothing and safety glasses.
Warning and caution messages
Carefully read all warnings and cautions and observe them at all
times.
A Warning message is used in the text when failure to observe
instructions or precautions could result in death or injury. Warnings
have the following format:
WARNING
Hazard Type
Nature of the hazard, information on how to avoid the hazard, and possible
consequences if you don’t.
The triangular symbols that appear in conjunction with warnings are
outlined in the next section.

Safety Practices and Hazards
8Cary 50 Spectrophotometer Hardware Manual
A Caution message is used when failure to observe instructions could
result in damage to equipment (Agilent-supplied and/or other
associated equipment). Cautions have the following format:
CAUTION
Caution information appears here.
Information symbols
The following triangular symbols appear in conjunction with
warnings on the spectrometer and associated documentation. The
hazard they depict is shown below each symbol:
Broken glass
Corrosive liquid
Electrical shock
Eye hazard
Fire hazard
Heavy weight
(danger to feet)
Heavy weight
(danger to hands)
Hot surface
Noxious gas
The following symbol may be used on warning labels attached to the
instrument. When you see this symbol, refer to the relevant operation
or service manual for the correct procedure referred to by that
warning label.

Safety Practices and Hazards
Cary 50 Spectrophotometer Hardware Manual 9
The following symbols also appear on the instrument or in the
documentation:
Xe
Indicates that a high voltage xenon flash lamp is present.
Indicates viewing hole to check the operation of the Xenon flash
lamp.
When attached to the rear of the instrument, indicates that the
product complies with the requirements of one or more EU
directives.
When attached to the rear of the product, indicates that the product
has been certified (evaluated) to CSA 1010.1 and UL 3101-1.
Color coding
The various indicator lights appearing on Agilent instruments and
associated accessories are color-coded to represent the status of the
instrument or accessory.
The green indicator lamp on the front of the Cary 50 indicates the
instrument is powered up (that is, the computer is switched on) and
is in normal/standby condition. When the indicator lamp is flashing,
this indicates the instrument is busy.

Safety Practices and Hazards
10 Cary 50 Spectrophotometer Hardware Manual
CE compliance
Agilent Cary 50 instruments have been designed to comply with the
requirements of the Electromagnetic Compatibility (EMC) Directive
and the Low Voltage (electrical safety) Directive (commonly referred
to as the LVD) of the European Union. Agilent has confirmed that
each product complies with the relevant directives by testing a
prototype against the prescribed EN (European Norm) standards.
Proof that a product complies with the directives is indicated by:
The CE marking appearing on the rear of the product.
The documentation package that accompanies the product,
containing a copy of the Declaration of Conformity. This
declaration is the legal declaration by Agilent that the product
complies with the directives and also shows the EN standards to
which the product was tested to demonstrate compliance.

Introduction
Cary 50 Spectrophotometer Hardware Manual 11

2. Introduction
Installation requirements 11
Documentation 11
Specifications 12
Installation requirements
Before receiving your instrument, you will have been provided with a
Cary 50 PReinstallation Manual (publication number 8510161800),
which describes the environmental and operating requirements of
the Cary 50 spectrophotometer. You must prepare your laboratory
according to these instructions before the Cary 50 can be installed.
You should keep the pReinstallation manual for future reference. If
you have misplaced your copy, you can obtain a free replacement
from your local Agilent office. Alternatively, download a PDF from
the Agilent Web site, www.agilent.com
Documentation
You have been provided with the following documentation to help
you set up and operate your Agilent Cary 50 spectrophotometer:
Installation guides (one for each supported operating system),
with information on unpacking the instrument, installing the
interface card in the computer and setting up the system.
This operation manual, with safety practices and hazards
information, instructions for installing and maintaining the
components of the Cary 50, and troubleshooting information.

Introduction
12 Cary 50 Spectrophotometer Hardware Manual
Cary WinUV Software Manual (publication number 8510162500),
with instructions for installing the Cary WinUV software, an
overview of the software and software-related troubleshooting
information.
Extensive online Help (provided with the Cary WinUV software)
containing context-sensitive Help, step-by-step instructions for
frequently performed analyses and instructions for using any
accessories you ordered.
Conventions
The following conventions have been used throughout the
documentation:
Menus, menu items, buttons and check boxes have been typed in
bold. For example, ‘click OK’ and ‘From the Edit menu, choose
Copy’.
ALL CAPITALS indicate keyboard commands. For example,
‘Press ENTER’ and ‘Press SHIFT+F3’.
NOTE
Throughout this manual, UV Dissolution and UV Fiber Optic Dissolution users
should replace „Cary WinUV Software‟ with „UV Dissolution Software‟ or „UV
Fiber Optic Dissolution Software‟ respectively.
Specifications
The instrument is suitable for indoor use only and is classified
Pollution degree 2 and Installation Category II (EN 61010-1).

Introduction
Cary 50 Spectrophotometer Hardware Manual 13
Environmental
Table 1. Suitable conditions during instrument transportation, non-operation and operation
Condition
Altitude (m, ft)
Temperature
(°C, °F)
Relative humidity,
non-condensing (%)
Non-operating (transport)
0–3050, 0–10,000
5–45, 41–113
20–80
Operating within performance
specifications
0–3050, 0–10,000
10–35, 50–95
8–80
NOTE
For optimum analytical performance, it is recommended that the ambient
temperature of the laboratory be between 20 and 25 °C and be held constant to
within ±2 °C throughout the entire working day.
Weights and dimensions
Weights
Packed: 23 kg (50.7 lb)
Unpacked: 21 kg (46 lb)
Dimensions (width x depth x height)
Packed: 615 x 710 x 360 mm (24 x 28 x 14 in)
Unpacked: 590 x 500 x 200 mm (23 x 20 x 8 in)
Computer power supply
To ensure safe operation of the instrument, the switching power
supply in the computer must comply with standard IEC 950 or
equivalent.

Introduction
14 Cary 50 Spectrophotometer Hardware Manual
The power requirements of the Cary 50 must be met by the computer
power supply and the following capacity (in addition to what is
required by the computer itself) is required:
+5 V DC <1 A
+12 V DC <1.5 A
-12 V DC <0.25 A
a total of 26 W for the instrument
Allowance must also be made for external accessories powered by
+12 V DC.
Operating motor-driven accessories will increase the +12 V current
by a further 2 A (a maximum of 24 W for accessories).
Agilent recommends a computer power supply rating of 220 W as a
minimum.
Connections
3.5 mm phono jack socket in the left side of the sample
compartment for accessories.
8-pin DIN connector in the left side of the sample compartment
for the diode detector.
25-pin D-range connector in the right side of the sample
compartment for accessories (optional).
NOTE
If an external cooling air supply system is required, an air inlet duct attachment
(part number 0110595300) must be ordered with the instrument.

Installation
Cary 50 Spectrophotometer Hardware Manual 15
3. Installation
Cell holder 16
Uninstalling the Cary WinUV software 19
Uninstalling the Cary 50 driver 19
The Agilent Cary 50 spectrophotometer is designed to be completely
customer-installed. Instructions for setting up the system are
included in the installation guides supplied with the instrument. You
should select the one appropriate for the operating system you are
using on your computer.
WARNING
Heavy Weight Hazard
The Cary 50 weighs over 20 kg (44 lb). To avoid injury to personnel or damage
to equipment, always use two or more people when lifting or carrying the
instrument. NEVER attempt to lift the instrument alone.
Details for unpacking the instrument and what to do in case it has
been damaged in transit are outlined in the pReinstallation manual.
Following the instructions in the Cary 50 Installation Instructions
(Publication Number 8510185100) you should have:
Unpacked the spectrophotometer and placed it on the intended
workbench.
Connected the computer to the power supply.
Installed the Cary WinUV software.
Installed the instrument interface card in the computer (if you
supplied your own computer).

Installation
16 Cary 50 Spectrophotometer Hardware Manual
Installed the Accessory Cable kit (if ordered).
Connected the instrument to the computer.
Completed the instrument performance tests.
This chapter describes how to install the sample holders used with
the Cary 50, and how to uninstall the software and driver, should you
ever need to. Instructions for installing/replacing the lamp module
are included in the Maintenance chapter.
Cell holder
A microcell holder (see Figure 1) is supplied as standard with the
Cary 50. Follow these instructions to install and align it.
Figure 1. Cell holder base (left) and microcell holder (right)
1) Locating holes 2) Cell holder base thumbscrew
3) Cell lifter 4) Microcell holder thumbscrew
5) Adjustment screw 6) Hexagonal ball driver
To install the microcell holder:
1Place a microcell in the microcell holder (if the cell has ground
glass sides or sides featuring the Cary logo, hold the cell by these
sides) and check that the cell aperture is vertically centered in
the cell holder aperture.

Installation
Cary 50 Spectrophotometer Hardware Manual 17
2If the cell aperture is not at the correct height, remove the cell
and adjust the grub screw in the microcell holder accordingly
using the 2.5 millimeter hexagonal ball driver. Replace the cell in
the microcell holder.
3Slide back the sample compartment lid (refer to Figure 2).
1
2
Figure 2. Cary 50 showing the sample compartment lid (1) and front panel (2)
4Remove the sample compartment front panel (refer to Figure 2).
5If not already fitted, install the cell holder base in the sample
compartment as follows:
aPlace the cell holder base in the sample compartment,
aligning the two locating holes over the two locating pins in
the floor of the sample compartment.
bFirmly tighten the thumbscrew on the cell holder base (item
2 in Figure 1).
6Place the microcell holder on the cell holder base, aligning the
holes in the microcell holder over the raised black knobs in the
cell holder base.
7Tighten the thumbscrew on the microcell holder.
You should now align the microcell holder.

Installation
18 Cary 50 Spectrophotometer Hardware Manual
Alignment
To align the microcell holder:
1Start the Align application by clicking the Windows® Start button
and choosing (All) Programs, Agilent, Cary WinUV (or Agilent,
UV/UV FO Dissolution) then Align.
2Click the Cary tab.
3Under ‘Instrument Parameters’, set the wavelength to 0 nm
(white light) by selecting Zero Order.
4Click Apply. The green power indicator on the instrument should
start flashing to indicate that the instrument is active.
5Place a cell in the microcell holder (if you have not already done
so).
6Place a small piece of white paper in the light path to the right of
the cell. If the beam appears as though it will strike the cell
aperture, move the paper to the left of the cell and check that the
beam is passing through the cell. (If the beam does not appear as
though it will pass through the cell, adjust the height of the cell as
described on Page 17.)
7Using the 2.5 millimeter hexagonal ball driver, adjust the
microcell holder adjustment screw (item 5 in Figure 1) and note
the intensity of the light striking the paper. Continue to adjust
the adjustment screw until the beam hitting the paper appears
the most intense.
NOTE
You may need to dim the room lights to see the light beam.
Other sample holders
Other sample holders are available for use with the Cary 50, such as
the Solid Sample Holder. Instructions for their use are included in
the online Help provided with the Cary WinUV software. Refer to the
Cary WinUV Software Manual (part number 8510162500) for details
on using the online Help.

Installation
Cary 50 Spectrophotometer Hardware Manual 19
Uninstalling the Cary WinUV software
NOTE
For Pharma software, you will need to contact your local Agilent office, as a
service call will be required to uninstall Cary WinUV Pharma software.
To uninstall the Cary WinUV Software:
1Click the Windows Start button and choose Control Panel >
Add/Remove Programs.
2Scroll through the list on the Install/Uninstall tabbed page until
you find ‘Agilent Cary WinUV’.
3Click Add/Remove and then Yes. Follow the instructions on the
screen.
4Repeat Step 3 for the Cary WinUV Help.
Uninstalling the Cary 50 driver
To uninstall the Cary 50 driver:
1Click the Windows Start button and choose Control Panel >
System.
2On the Hardware tab, select Device Manager, and click the plus
(‘+’) icon next to ‘Other’ devices.
3Right-click Cary 50 PCI Rev03 and then click Uninstall.
4Restart the computer.

Installation
20 Cary 50 Spectrophotometer Hardware Manual
This page is intentionally left blank.