Agilent Technologies Cary 50 User manual

Cary 50
Spectrophotometer
Hardware Manual

2Cary 50 Spectrophotometer Hardware Manual
Notices
© Agilent Technologies, Inc. 1998,
1999, 2002, 2004, 2006, 2010 and 2011
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Manual Part Number
8510159600
Edition
Ninth edition, March 2011
Printed in Australia
Agilent Technologies, Inc.
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described in this document are
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Safety Notices
A CAUTION notice denotes a hazard.
It calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION notice
until the indicated conditions are fully
understood and met.
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or the
like that, if not correctly performed or
adhered to, could result in personal
injury or death. Do not proceed
beyond a WARNING notice until the
indicated conditions are fully
understood and met.
WARNING
CAUTION

Contents
Cary 50 Spectrophotometer Hardware Manual 3
Contents
1. Safety Practices and Hazards 5
Lamp module 6
Electrical hazards 6
Modules, covers and panels 6
Other precautions 7
Warning and caution messages 7
Information symbols 8
Color coding 9
CE compliance 10
2. Introduction 11
Installation requirements11
Documentation 11
Conventions 12
Specifications 12
Environmental 13
Weights and dimensions13
Computer power supply 13
Connections 14
3. Installation 15
Cell holder 16
Alignment 18
Other sample holders 18

Contents
4Cary 50 Spectrophotometer Hardware Manual
Uninstalling the Cary WinUV software 19
Uninstalling the Cary 50 driver 19
4. Maintenance 21
Cleaning 22
Lamp module 22
Replacing 22
Aligning the source mirror 25
5. Troubleshooting 27
Windows XP service or driver failure 27
Access denied during installation28
FCary service failed to start 29
No green light indicating that the Cary 50 is powered 30
Start button replaced with Connect button 30
Hard disk power supply connection 32
Absorbance is 10 and fluctuates wildly during a scan 32
Instrument performance testing 33
Wavelength calibration 35
6. Spare Parts 37

Safety Practices and Hazards
Cary 50 Spectrophotometer Hardware Manual 5
1. Safety Practices and Hazards
Lamp module 6
Electrical hazards 6
Modules, covers and panels 6
Other precautions 7
Warning and caution messages 7
Information symbols 8
Color coding 9
CE compliance 10
Your Agilent Cary 50 instrument and accessories have been carefully
designed so that when used properly, you have an accurate, fast,
flexible and safe analytical system.
If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be
impaired.
Information about safety practices appears throughout the
documentation (both hard copy and online) provided with your
instrument and accessories to help you safely operate the instrument
and accessories. Before using the instrument or accessories, you
must thoroughly read these safety practices. ALWAYS operate the
instrument and accessories in accordance with these safety practices.

Safety Practices and Hazards
6Cary 50 Spectrophotometer Hardware Manual
Lamp module
The lamp is enclosed in a self-contained module. This module
contains components operating at high voltages. To avoid electric
shock, NEVER disassemble the module.
When operating, the lamp module emits high-intensity light, which
can cause serious damage to eyes. To avoid eye damage, never
operate the lamp outside the instrument.
Electrical hazards
The Cary 50 spectrophotometer is powered by the computer
controlling the instrument. Safe operation of the instrument depends
on the integrity of the switching power supply of the computer. Your
computer must comply with IEC 60950.
Modules, covers and panels
The ONLY modules of the Cary 50 spectrophotometer that operators
and other unauthorized personnel are permitted to access are the
lamp module (on the underside of the instrument) and the sample
compartment. White circles indicate the screws you need to undo to
remove the lamp module. ALWAYS switch off the computer before
changing a lamp.
The only panel you are permitted to remove is the snap-out panel
covering the lamp mirror adjustment screws on the front of the
instrument.
Any other panels or covers that are retained by screws on the
spectrophotometer and accessories may be opened ONLY by Agilent-
trained, Agilent-qualified, or Agilent-approved customer support
representatives. Consult the manuals or product labels supplied with
your computer, monitor and printer to determine which parts are
operator-accessible.
NOTE
The safety classification is given as Safety Class III (EN 61010-1).

Safety Practices and Hazards
Cary 50 Spectrophotometer Hardware Manual 7
Other precautions
Do not block any ventilation grilles present on the computer. Consult
the manuals supplied with your computer, monitor and printer for
their specific ventilation requirements.
Use of the Cary 50 system and accessories may involve materials,
solvents and solutions that are flammable, corrosive, toxic or
otherwise hazardous.
Careless, improper, or unskilled use of such materials, solvents and
solutions can create explosion hazards, fire hazards, toxicity and
other hazards which can result in death, serious personal injury, and
damage to equipment and property.
ALWAYS ensure that laboratory safety practices governing the use,
handling and disposal of such materials are strictly observed. These
safety practices should include the wearing of appropriate safety
clothing and safety glasses.
Warning and caution messages
Carefully read all warnings and cautions and observe them at all
times.
A Warning message is used in the text when failure to observe
instructions or precautions could result in death or injury. Warnings
have the following format:
WARNING
Hazard Type
Nature of the hazard, information on how to avoid the hazard, and possible
consequences if you don’t.
The triangular symbols that appear in conjunction with warnings are
outlined in the next section.

Safety Practices and Hazards
8Cary 50 Spectrophotometer Hardware Manual
A Caution message is used when failure to observe instructions could
result in damage to equipment (Agilent-supplied and/or other
associated equipment). Cautions have the following format:
CAUTION
Caution information appears here.
Information symbols
The following triangular symbols appear in conjunction with
warnings on the spectrometer and associated documentation. The
hazard they depict is shown below each symbol:
Broken glass
Corrosive liquid
Electrical shock
Eye hazard
Fire hazard
Heavy weight
(danger to feet)
Heavy weight
(danger to hands)
Hot surface
Noxious gas
The following symbol may be used on warning labels attached to the
instrument. When you see this symbol, refer to the relevant operation
or service manual for the correct procedure referred to by that
warning label.

Safety Practices and Hazards
Cary 50 Spectrophotometer Hardware Manual 9
The following symbols also appear on the instrument or in the
documentation:
Xe
Indicates that a high voltage xenon flash lamp is present.
Indicates viewing hole to check the operation of the Xenon flash
lamp.
When attached to the rear of the instrument, indicates that the
product complies with the requirements of one or more EU
directives.
When attached to the rear of the product, indicates that the product
has been certified (evaluated) to CSA 1010.1 and UL 3101-1.
Color coding
The various indicator lights appearing on Agilent instruments and
associated accessories are color-coded to represent the status of the
instrument or accessory.
The green indicator lamp on the front of the Cary 50 indicates the
instrument is powered up (that is, the computer is switched on) and
is in normal/standby condition. When the indicator lamp is flashing,
this indicates the instrument is busy.

Safety Practices and Hazards
10 Cary 50 Spectrophotometer Hardware Manual
CE compliance
Agilent Cary 50 instruments have been designed to comply with the
requirements of the Electromagnetic Compatibility (EMC) Directive
and the Low Voltage (electrical safety) Directive (commonly referred
to as the LVD) of the European Union. Agilent has confirmed that
each product complies with the relevant directives by testing a
prototype against the prescribed EN (European Norm) standards.
Proof that a product complies with the directives is indicated by:
The CE marking appearing on the rear of the product.
The documentation package that accompanies the product,
containing a copy of the Declaration of Conformity. This
declaration is the legal declaration by Agilent that the product
complies with the directives and also shows the EN standards to
which the product was tested to demonstrate compliance.