Agilent Technologies Cary Eclipse User manual

Cary Eclipse
Fluorescence
Spectrophotometer
User’s Guide

2 Cary Eclipse Fluorescence Spectrophotometer User’s Guide
Notices
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2000 and 2011-2013, 2015, 2016
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Manual Part Number
8510175800
Edition
Seventh edition, September 2016
Printed in Malaysia
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Ltd
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Safety Notices
A CAUTION notice denotes a hazard.
It calls attention to an operating
procedure, practice, or the like that, if
not correctly performed or adhered to,
could result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION notice
until the indicated conditions are fully
understood and met.
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or the
like that, if not correctly performed or
adhered to, could result in personal
injury or death. Do not proceed
beyond a WARNING notice until the
indicated conditions are fully
understood and met.
WARNING
CAUTION

Contents
Cary Eclipse Fluorescence Spectrophotometer User’s Guide 3
Contents
1. Safety Practices and Hazards 7
Verifying Safe State 7
Lamp module 8
Ultraviolet radiation 8
Electrical hazards 9
Panels, covers and modules 10
Other precautions 10
Warning and information symbols 11
Color coding 13
CE compliance 13
Electromagnetic compatibility 14
EN55011/CISPR11 14
ICES/NMB-001 15
South Korean Class A EMC declaration 15
2. Introduction 17
Installation requirements 17
Documentation 17
Conventions 18
Specifications 18
Environmental 18
Weights and dimensions 19
Power 19

Contents
4 Cary Eclipse Fluorescence Spectrophotometer User’s Guide
Connections 19
Fuses 20
Nitrogen supply 20
Computer placement 20
3. Installation 21
Single cell holder 22
Aligning 24
Other sample holders 25
Purging 25
Nitrogen 25
Pressure regulator and gauge 25
Supply tubing 25
Gas manifold 26
Flowmeter 26
Installing the software 26
Installing the USB-GPIB-HS Converter 28
Registering the Cary Eclipse software 28
Uninstalling the software 29
Software Status Bulletin 29
4. Maintenance and Spare Parts 31
Cleaning 32
Lamp module 32
Replacing 32
Aligning 35
Fuses 38

Contents
Cary Eclipse Fluorescence Spectrophotometer User’s Guide 5
Replacing 38
Checking 39
Spare Parts 39
5. Troubleshooting 41
Instrument offline 42
Connect button instead of Start 42
Instrument performance testing 43

Contents
6 Cary Eclipse Fluorescence Spectrophotometer User’s Guide
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Safety Practices and Hazards
Cary Eclipse Fluorescence Spectrophotometer User’s Guide 7
1. Safety Practices and Hazards
Verifying Safe State 7
Lamp module 8
Ultraviolet radiation 8
Electrical hazards 9
Panels, covers and modules 10
Other precautions 10
Warning and information symbols 11
Color coding 13
CE compliance 13
Electromagnetic compatibility 14
Your Agilent Cary Eclipse instrument and accessories have been
carefully designed so that when used properly you have an accurate,
fast, flexible and safe analytical system.
If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be
impaired.
Information on safety practices appears throughout the
documentation (both hard copy and online) provided with your
instrument and accessories. Before using the instrument or
accessories, you must thoroughly read these safety practices.
Observe all relevant safety practices at all times.
Verifying Safe State
The following general safety precautions must be observed during all
phases of operation, maintenance and service of this instrument.

Safety Practices and Hazards
8 Cary Eclipse Fluorescence Spectrophotometer User’s Guide
To ensure continued safety of the instrument after maintenance or
service procedures verify the instrument is returned to a safe state
for the user. This includes running performance checks to verify the
instruments safety systems are functioning correctly. Check the
general condition of the instrument during operation for wear or
signs of corrosion that are likely to inhibit function or safety.
Failure to comply with these precautions or with specific warnings
elsewhere in this manual violates safety standards of design,
manufacture, and intended use of the instrument. Agilent
Technologies assumes no liability for the customer’s failure to
comply with these requirements.
Lamp module
The lamp is enclosed in a self-contained module. This module
contains components operating at high voltages. To avoid electric
shock, NEVER disassemble the module.
When operating, the lamp module emits high intensity visible and
ultraviolet (UV) radiation that can cause serious damage to eyes. To
avoid eye damage, never operate the lamp outside the instrument.
WARNING
Eye and Shock Hazard
High voltage Xe flashlamp operating at 1260 kPa. To avoid electric shock or
eye damage, NEVER disassemble the module or look down the hole.
La lampe Xe haut voltage fonctionne à une pression de 1260 kpa. Pour éviter
tout risque électrique et des problèmes oculaires ne jamais ouvrir le module
ou regarder à l’intérieur.
Ultraviolet radiation
Hazardous ultraviolet (UV) radiation is emitted by the xenon flash
lamp in the instrument. This radiation can cause serious damage to
eyes. Never look directly at the UV source lamp.

Safety Practices and Hazards
Cary Eclipse Fluorescence Spectrophotometer User’s Guide 9
Electrical hazards
The Cary Eclipse system and some accessories contain electrical
circuits, devices, and components operating at dangerous voltages.
Contact with these circuits, devices and components can cause death,
serious injury, or painful electrical shock.
Panels or covers which are retained by screws on the
spectrophotometer and accessories and carry a warning may be
opened ONLY by Agilent-trained, Agilent-qualified, or Agilent-
approved customer service representatives. Consult the manuals or
product labels supplied with your computer, monitor and printer to
determine which parts are operator-accessible.
Good grounding is essential to avoid a potentially serious electric
shock hazard. Ensure that there is an integral ground connection
between the metal base of the spectrophotometer and accessories
and the three pin earth-grounded receptacle. Consult the manuals or
product labels supplied with your computer, monitor and printer for
their grounding requirements.
NOTE The safety classification is given as Equipment Class I.
Application of the wrong supply voltage can create a fire hazard and
a potentially serious shock hazard, and could seriously damage the
Cary Eclipse system, accessories and any attached ancillary
equipment.
Do not connect the Cary Eclipse, your computer, monitor, printer or
accessories to the mains power supply until you have made sure that
they are correctly set for the mains power supply in the specific
outlet in your laboratory to which the equipment will be connected.
Consult the manuals supplied with your computer, monitor and
printer for their specific voltage requirements.
Replace blown fuses with fuses of the size and rating as stipulated in
the text adjacent to the fuse holder or in the manuals where listed.
Replace or repair faulty or frayed insulation on power cords.
Do not replace the power cord with one of a lower rating than
specified. Use only Agilent supplied power cords for your country.

Safety Practices and Hazards
10 Cary Eclipse Fluorescence Spectrophotometer User’s Guide
WARNING
Shock Hazard
To avoid electrical shock this equipment must be disconnected from the
mains supply before servicing.
Panels, covers and modules
You are permitted to remove the top colored cover to:
 Replace the lamp module.
 Adjust the lamp module.
Any other panels or covers, which are retained by screws on the
spectrophotometer and accessories, may be opened ONLY by Agilent-
trained, Agilent-qualified, or Agilent-approved service engineers.
Consult the manuals or product labels supplied with your computer,
monitor and printer to determine which parts are operator-
accessible.
Operators and other unauthorized personnel are permitted access
ONLY to the lamp module and the sample compartment of the Cary
Eclipse. ALWAYS switch off the instrument and disconnect the mains
cord before changing a lamp module.
Other precautions
The lamp module operates at a high temperature, and touching it
may result in burns. Before replacing a lamp module that has been
operating, switch off the Cary Eclipse and ensure that the lamp
module has cooled, or protect your fingers from burns.
Do not block the ventilation grilles on the spectrophotometer and
accessories. Consult the manuals supplied with your computer,
monitor and printer for their specific ventilation requirements.
Use of the Cary Eclipse system and accessories may involve
materials, solvents and solutions, which are flammable, corrosive,
toxic or otherwise hazardous.