Agilent Technologies 2200 TapeStation User manual

Agilent Technologies
Agilent High Sensitivity D1000
ScreenTape System Quick Guide
The Agilent 2200 TapeStation system is an automated platform for simpler, faster and more reliable
electrophoresis. It is made up of 3 elements:
• 2200 TapeStation system (G2964AA) or 2200 TapeStation Nucleic Acid system (G2965AA)
• High Sensitivity D1000 ScreenTape (5067- 5584) with High Sensitivity D1000 Reagents (5067- 5585)
• Agilent Software packages (2200 TapeStation Controller Software, and TapeStation Analysis Software)
Kits
The High Sensitivity D1000 ScreenTape system is designed for analysing DNA molecules from 35 – 1000 bp.
Specifications
Analytical Specifications High Sensitivity D1000 ScreenTape assay and reagents
Sizing Range 35 – 1000 bp
Typical Resolution 35 – 300 bp: 15 %; 300 – 1000 bp: 10 %
Sensitivity1
1signal- to- noise >3 (single peak)
5 pg/µL
Sizing Precision 5 % CV
Sizing Accuracy2
2Sizing Accuracy for electronic ladder: ±20%
±10 %
Quantitative Precision 15 % CV
Quantitative Accuracy3
3Measured against 2100 Bioanalyzer system
±20 %
Quantitative Range 10 – 1000 pg/µL
Maximum sample buffer strength 7 mM KCl, 20 mM Phosphate Buffer, 20 mM Guanidine-HCl, 80 mM NaCl, 20 mM Acetate
Physical Specifications
Analysis Time 16 samples: <20 min; 96 samples: ≈100 min
Samples per consumable 16
Sample volume required 2 µL
Kit stability 4 months
Kit size 112 samples

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Agilent High Sensitivity D1000 ScreenTape System Quick Guide
Storage Conditions
•Reagent vials: 2 – 8 °C (36 – 46 °F)
•The ScreenTape device: 2 – 8 °C (36 – 46 °F) (if you run less than 16 lanes, store used ScreenTape
device upright at 2 – 8 °C (36 – 46 °F) for a maximum of 2 weeks.)
•Never freeze the ScreenTape device - any ScreenTape device which is accidentally frozen should be
discarded.
Kit Components
Additional Consumables Required for the 2200 TapeStation Instrument
•Loading tips (5067- 5152 or 5067- 5153)
•Optical Tube 8x Strip (401428) and Optical Cap 8x Strip (401425) or 96- well Sample Plates
(5067- 5150) and 96- well Plate Foil Seal (5067- 5154).
•Vortex mixer (See note below)
Additional Material Required (Not Supplied)
•Volumetric pipette
•Centrifuge
Safety Information
Part Number Name Color Amount
5067-5584 High Sensitivity D1000 ScreenTape 7 ScreenTape devices
5067-5585 High Sensitivity D1000 Reagents
•High Sensitivity D1000 Ladder
•High Sensitivity D1000 Sample Buffer
2 vials
20 µL
300 µL
5067-5587 High Sensitivity D1000 Ladder 1 vial
20 µL
NOTE
2200 TapeStation instruments are supplied with an optional IKA MS3 vortexer which
includes a 96-well plate adaptor suitable for both 96-well PCR plates and 8-way strips.
WARNING
Toxic a gents
The handling of solvents, samples and reagents can hold health and safety risks.
➔When using/handling the ScreenTape device and working with these substances observe
appropriate safety procedures (for example by wearing goggles, safety gloves and protective
clothing).
➔Always follow good laboratory practices and adhere to the guidelines established in your
laboratory.
➔Refer to product material safety datasheets for further information.
➔The volume of substances should be reduced to the minimum required for the analysis.

3
Agilent High Sensitivity D1000 ScreenTape System Quick Guide
General Information on Working with DNA
Essential Measurement Practices
CAUTION
Damage to the 2200 TapeStation instrument
➔Use only the recommended consumables and reagents with the 2200 TapeStation system.
NOTE
•For best results ensure that all reagents are allowed to equilibrate to room temperature
for 30 min prior to use.
•When pipetting Sample Buffer, ensure that excess buffer droplets are removed from the
tip before transfer to the sample tubes. Care must be taken due to the viscosity of
Sample Buffer.
•When pipetting small volumes ensure that no sample remains within the tip.
•When adding sample buffer to sample, please ensure that they are mixed correctly. To
achieve this, gently mix several times with additional pipetting, then cap the tubes,
vortex mix using IKA vortexer and adaptor at 2000 rpm for 1 min.
•Briefly centrifuge to collect the contents at the base of the tubes.
•Improper mixing can lead to quantification errors.
Environmental
conditions
•Optimal operating temperature: 20 °C (68 °F)
•Ambient operating temperature: 17 - 37 °C (63 - 99 °F)
Steps before use on
the TapeStation
instrument
•Equilibrate each vial to room temperature.
•Vortex mix each vial and briefly spin.
•'Flick' ScreenTape device to eliminate bubbles in the separation channel, which could interfere
with sample loading.
Steps during sample
preparation
•Keep reagents at room temperature during sample preparation.
Storage after use on
the TapeStation
instrument
•Store all reagent vials and ScreenTape devices at 2 – 8 °C (36 – 46 °F)
•Never store reagent vials or ScreenTape devices at room temperature or below 0 °C (32 °F).
•If you run less than 16 lanes, store used ScreenTape device upright at 2 – 8 °C (36 – 46 °F) for a
maximum of 2 weeks.
Pipette carefully •Always pipette reagents against the side of the sample tube.
•If using a standard pipette ensure that no residual material is left on the outside of the tip.
Mix properly after
each pipetting step
•Mix = Vortex the PCR tubes or 96-well plate using Agilent approved IKA vortexer and adaptor at
2000 rpm for 1 min.
•Spin = Move the samples to the bottom of the tubes/wells by pulsing in a centrifuge.

Agilent High Sensitivity D1000 ScreenTape System Quick Guide
*G2964-90131*
*G2964-90131*
G2964-90131
Part Number: G2964-90131 Rev. D
Edition 09/2015
Printed in Germany
© Agilent Technologies, Inc. 2013-2014, 2015
Agilent Technologies
Hewlett-Packard-Straße 8
76337 Waldbronn, Germany
Prepare TapeStation System HSD1000
1
Launch the 2200 TapeStation Controller Software.
2 Load High Sensitivity D1000 ScreenTape device and loading tips into the 2200 TapeStation instrument.
Sample Preparation High Sensitivity D1000 ScreenTape Assay
1Allow reagents to equilibrate at room temperature for 30 min
2Vortex mix before use
3If running ladder, prepare by mixing 2 µL High Sensitivity D1000 Sample Buffer ( ) with 2 µL High
Sensitivity D1000 Ladder ( )
4Prepare sample by mixing 2 µL High Sensitivity D1000 Sample Buffer ( ) with 2 µL DNA sample
5Spin down, then vortex using IKA vortexer and adaptor at 2000 rpm for 1 min
6Spin down to position the sample at the bottom of the tube.
Sample Analysis
1Load samples into the 2200 TapeStation instrument.
2Select the required samples on the 2200 TapeStation Controller Software.
3Click Start and specify a filename with which to save your results.
Limited Use Label License
Some products within this system contain SYBR® Green I, which is licensed from Molecular Probes Inc. for use in
research and development only. SYBR® is a registered trademark of Molecular Probes, Inc.
For Research Use Only
Not for use in Diagnostic Procedures.
Technical Support
For technical support, please visit www.agilent.com/genomics/contact
Further Information
Visit Agilent Technologies` web site. It offers useful information, support and current developments
about the products and technology: www.agilent.com/genomics/tapestation
Parts required p/n Description
5067-5584 High Sensitivity D1000 ScreenTape
Parts required p/n Description
5067-5585 High Sensitivity D1000 Reagents
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