Agilent Technologies 1100 Series User manual

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Agilent 1100 Series
Diode Array and
Multiple Wavelength
Detector SL
User Manual

2 1100 Series DAD and MWD User Manual
Notices
© Agilent Technologies, Inc. 2005
No part of this manual may be reproduced in
any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G1315-90010
Edition
Edition 06/2005
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Manual Structure
The Service Manual G1315-90110 (English)
contains the complete information about
the Agilent 1100 Series Diode Array and
Multiple Wavelength Detector SL. It is avail-
able as Adobe Reader file (PDF) only.
The User Manual G1315-90010 (English)
and its localized versions contain a subset
of the Service Manual and is shipped with
the detector in printed matter.
All manuals will be also on the Product CD
G1315-60030 (localized versions when
available) as Adobe Reader file (PDF). This
CD is shipped with the detector.
Latest versions of the manuals can be
obtained from the Agilent web.
Warranty
The material contained in this docu-
ment is provided “as is,” and is sub-
ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
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either express or implied, with regard
to this manual and any information
contained herein, including but not
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Safety Notices
CAUTION
A CAUTION notice denotes a haz-
ard. It calls attention to an operat-
ing procedure, practice, or the like
that, if not correctly performed or
adhered to, could result in damage
to the product or loss of important
data. Do not proceed beyond a
CAUTION notice until the indicated
conditions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could result
in personal injury or death. Do not
proceed beyond a WARNING
notice until the indicated condi-
tions are fully understood and
met.

1100 Series DAD and MWD User Manual 3
In This Manual…
This manual contains information for using and servicing your
Diode Array and Multiple Wavelength Detector SL.
1Site Requirements and Specifications
This chapter provides information on environmental
requirements, physical and performance specifications.
2Installing the Detector
This chapter provides information on unpacking, checking on
completeness, stack considerations and installation of the
detector.
3LAN Configuration
This chapter provides information on connecting the detector to
the Agilent ChemStation PC.
4Using the Detector
This chapter provides information on how to set up the detector
for an analysis and explains the basic settings.
5How to optimize the Detector
This chapter provides information on how to optimize the
detector.
6Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and
diagnostic features.s
7Maintenance and Repair
This chapter provides general information on repairing the
detector.
9Maintenance
This chapter describes the maintenance of the detector.

4 1100 Series DAD and MWD User Manual
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Control Module Screens
This chapter provides an overview on the control module
screens.
Annex A Appendix
This chapter provides addition information on safety, legal and
web.

1100 Series DAD and MWD User Manual 5
Content
1 Site Requirements and Specifications
Site Requirements 12
Physical Specifications 15
Performance Specifications 16
2 Installing the Detector
Unpacking the Detector 20
Damaged Packaging 20
Delivery Checklist 20
Optimizing the Stack Configuration 22
Installing the Detector 24
Flow Connections to the Detector 27
Setting up the LAN access 30
3 LAN Configuration
What you have to do first 32
TCP/IP parameter configuration 33
Configuration Switches 34
Initialization mode selection 35
Link configuration selection 39
Automatic configuration with Bootp 40
Configuring the Agilent Bootp service program 40
Storing the settings permanently with Bootp 45
Manual Configuration 46
With Telnet 47
With Handheld Controller (G1323B) 51

6 1100 Series DAD and MWD User Manual
PC and Agilent ChemStation Setup 54
PC Setup for Local Configuration 54
Agilent ChemStation Setup 55
4 Using the Detector
Setting up an Analysis 58
Before Using the System 58
Requirements and Conditions 60
Optimization of the System 62
Preparing the HPLC System 63
Running the Sample and Verifying the Results 72
Special Settings of the Detector 73
Control Settings 73
Configuration Settings 74
Online Spectra 75
Run Recovery Settings 76
Analog Output Settings 79
Spectrum Settings 80
Peakwidth Settings 82
Slit Settings 83
Margin for Negative Absorbance Settings 84
Optimizing the Detector 84
Special Setups with Multiple DAD/MWDs 85
Two detectors of same type (e.g. G1315C and G1315C) 85
Two detectors of similar type (e.g. G1315C and G1315A/B) 85
5 How to optimize the Detector
Optimizing the Detector Performance 88
Optimization Overview 89
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 91
Flow Cell Path Length 91

1100 Series DAD and MWD User Manual 7
Peak width (response time) 93
Sample and Reference Wavelength and Bandwidth 94
Slit Width 97
Optimizing Spectral Acquisition (DAD only) 100
Margin for Negative Absorbance 101
Optimizing Selectivity 102
Quantifying Coeluting Peaks by Peak Suppression 102
Ratio Qualifiers for Selective Detection of Compound
Classes 104
6 Troubleshooting and Diagnostics
Overview of the Detector’s Indicators and Test Functions 108
Status Indicators 109
Power Supply Indicator 109
Detector Status Indicator 110
7 Maintenance and Repair
Introduction to Maintenance and Repair 112
Simple Repairs - Maintenance 112
Exchanging Internal Parts - Repair 112
Warnings and Cautions 113
Cleaning the Detector 114
Using the ESD Strap 115
8 Maintenance
Overview of Maintenance 118
Exchanging a Lamp 119
Exchanging a Flow Cell 121
Repairing the Standard or Semi-Micro Flow Cell 124
Repairing the High Pressure Flow Cell 129

8 1100 Series DAD and MWD User Manual
Replacing Capillaries on a Standard Flow Cell 131
Replacing Capillaries on a Semi-Micro and High Pressure Flow
Cell 136
Nano Flow Cell - Replacing or Cleaning 139
Cleaning or Exchanging the Holmium Oxide Filter 144
Correcting Leaks 147
Replacing Leak Handling System Parts 148
Replacing the CompactFlash Card 149
Replacing the Detector’s Firmware 150
9 Parts and Materials for Maintenance
Overview of Maintenance Parts 154
Standard Flow Cell 156
Semi-Micro Flow Cell 158
High Pressure Flow Cell 160
Nano Flow Cells 162
Accessory Kit 166
10 Control Module Screens
Major keys on the Agilent 1100 Control Module 170
Control Module - Unsupported Module 171
Screens available from the Analysis screen 172
Screens available from the System screen 182
Screens available from the Records screen 185
Diagnostics and Tests 192

1100 Series DAD and MWD User Manual 9
11 Appendix
General Safety Information 198
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 201
Radio Interference 202
Sound Emission 203
UV-Radiation 204
Solvent Information 205
Declaration of Conformity for HOX2 Filter 207
Agilent Technologies on Internet 208

10 1100 Series DAD and MWD User Manual

11
Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL
User Manual
Agilent Technologies
1
Site Requirements and Specifications
Site Requirements 12
Physical Specifications 15
Performance Specifications 16
This chapter provides information on environmental requirements, physical
and performance specifications.

12 1100 Series DAD and MWD User Manual
1 Site Requirements and Specifications
Site Requirements
A suitable environment is important to ensure optimal performance of the
detector.
Power Consideration
The detector power supply has wide ranging capabilities and accepts any line
voltage in the range mentioned in Table 1. Consequently, there is no voltage
selector in the rear of the detector. There are also no externally accessible
fuses, because automatic electronic fuses are implemented in the power
supply.
Power Cords
Different power cords are offered as options with the detector. The female end
of all power cords is identical. It plugs into the power-input socket at the rear
of the detector. The male end of each power cord is different and designed to
match the wall socket of a particular country or region.
WARNING To disconnect the detector from line, unplug the power cord. The power supply still
uses some power, even if the power switch on the front panel is turned off.
WARNING Shock hazard or damage of your instrumentation can result, if the devices are
connected to a line voltage higher than specified.
CAUTION Make sure to have easy access to the power cable of the instrument, in order to
disconnect the instrument from line.

1100 Series DAD and MWD User Manual 13
Site Requirements and Specifications 1
Bench Space
The detector dimensions and weight (see Table 1) allows you to place the
detector on almost any desk or laboratory bench. It needs an additional 2.5 cm
(1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the
rear for air circulation and electric connections.
If the bench should carry a Agilent 1100 Series system, make sure that the
bench is designed to bear the weight of all modules.
The detector should be operated in a horizontal position.
Environment
Your detector will work within the specifications at ambient temperatures and
relative humidity described in Table 1.
ASTM drift tests require a temperature change below 2 °C/hour (3.6 °F/hour)
over one hour period. Our published drift specification (refer also to
“Performance Specifications” on page 16) is based on these conditions. Larger
ambient temperature changes will result in larger drift.
Better drift performance depends on better control of the temperature
fluctuations. To realize the highest performance, minimize the frequency and
the amplitude of the temperature changes to below 1 °C/hour (1.8 °F/hour).
Turbulences around one minute or less can be ignored.
WARNING Never operate your instrumentation from a power outlet that has no ground
connection. Never use a power cord other than the Agilent Technologies power cord
designed for your region.
WARNING Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
CAUTION Do not store, ship or use your detector under conditions where temperature
fluctuations could cause condensation within the detector. Condensation will damage
the system electronics. If your detector was shipped in cold weather, leave it in its box
and allow it to warm up slowly to room temperature to avoid condensation.

14 1100 Series DAD and MWD User Manual
1 Site Requirements and Specifications
NOTE The G1315C DAD and G1365C MWD are designed to operate in a typical electromagnetic
environment (EN61326-1) where RF transmitters, such as mobile phones, should not be
used in close proximity.

1100 Series DAD and MWD User Manual 15
Site Requirements and Specifications 1
Physical Specifications
Table 1 Physical Specifications
Type Specification Comments
Weight 11.5 kg (26 lbs)
Dimensions
(width × depth × height)
345 × 435 × 140 mm
(13.5 × 17 × 5.5 inches)
Line voltage 100 – 240 VAC, ± 10 % Wide-ranging capability
Line frequency 50 or 60 Hz ± 5 %
Power consumption (G1315C/G1365C) 160 VA / 546 BTU/hour Maximum
Ambient operating temperature 0 – 55 °C (32 – 131 °F) .
Ambient non-operating temperature -40 – 70 °C (-4 – 158 °F)
Humidity < 95%, at 25 – 40 °C (77 – 104 °F) Non-condensing
Operating altitude Up to 2000 m (6500 ft)
Non-operating altitude Up to 4600 m (14950 ft) For storing the detector
Safety standards: IEC, CSA, UL, EN Installation category II, pollution degree 2

16 1100 Series DAD and MWD User Manual
1 Site Requirements and Specifications
Performance Specifications
Table 2 Performance Specifications Agilent 1100 Series DAD and MWD
Type Specification Comments
Detection type 1024-element photodiode array
Light source Deuterium and tungsten lamps The UV-lamp is equipped with I.D. tag
that holds lamp typical information.
Wavelength range 190 – 950 nm
Short term noise (ASTM)
Single and
Multi-Wavelength
±1×10
-5 AU at 254 and 750 nm See note on page 17
Drift 2 × 10-3 AU/hr at 254 nm See note on page 17
Linear absorbance range > 2 AU (upper limit) See note on page 17
Wavelength accuracy ± 1 nm Self-calibration with deuterium lines,
verification with holmium oxide filter
Wavelength bunching 1 – 400 nm Programmable in steps of 1 nm
Slit width 1, 2, 4 , 8, 16 nm Programmable slit
Diode width < 1 nm
Flow cells Standard: 13 µl volume, 10 mm cell path length
and 120 bar (1760 psi) pressure maximum
Semi-Micro: 5 µl volume, 6 mm cell path length
and 120 bar (1760 psi) pressure maximum
High pressure: 1.7 µl volume, 6 mm cell path
length and 400 bar (5880 psi) pressure maximum
500 nano: 0.5 µl volume, 10 mm cell path length
and 50 bar (725 psi) pressure maximum
80 nano: 0.5 µl volume, 10 mm cell path length and
50 bar (725 psi) pressure maximum
See “Optimization Overview” on
page 89
All flow cells are equipped with I.D.
tags that hold cell typical information.
Control and data evaluation Agilent ChemStation for LC (32-bit) Revision B.01.03 or above

1100 Series DAD and MWD User Manual 17
Site Requirements and Specifications 1
Analog outputs Recorder/integrator:100mVor1V,outputrange
0.001 – 2 AU, two outputs
Communications Controller-area network (CAN), RS-232C, APG
Remote: ready, start, stop and shut-down signals,
LAN
Safety and maintenance Extensive diagnostics, error detection and display
(through control module and ChemStation), leak
detection, safe leak handling, leak output signal
for shutdown of pumping system. Low voltages in
major maintenance areas.
GLP features Early maintenance feedback (EMF) for continuous
tracking of instrument usage in terms of lamp
burn time with user-setable limits and feedback
messages. Electronic records of maintenance and
errors. Verification of wavelength accuracy with
built-in holmium oxide filter.
Housing All materials recyclable.
Table 2 Performance Specifications Agilent 1100 Series DAD and MWD, continued
Type Specification Comments
NOTE ASTM: “Standard Practice for Variable Wavelength Photometric Detectors Used in Liquid
Chromatography”.
Reference conditions: cell path length 10 mm, response time 2 s, flow 1 ml/min LC-grade
Methanol, slit width 4 nm.
Linearity measured with caffeine at 265 nm.
For environmental conditions refer to “Environment” on page 13.

18 1100 Series DAD and MWD User Manual
1 Site Requirements and Specifications

19
Agilent 1100 Series Diode Array and Multiple Wavelength Detector SL
User Manual
Agilent Technologies
2
Installing the Detector
Unpacking the Detector 20
Optimizing the Stack Configuration 22
Installing the Detector 24
Flow Connections to the Detector 27
This chapter provides information on unpacking, checking on completeness,
stack considerations and installation of the detector.

20 1100 Series DAD and MWD User Manual
2 Installing the Detector
Unpacking the Detector
Damaged Packaging
If the delivery packaging shows signs of external damage, please call your
Agilent Technologies sales and service office immediately. Inform your service
representative that the detector may have been damaged during shipment.
Delivery Checklist
Ensure all parts and materials have been delivered with the detector. The
delivery checklist is shown below. Please report missing or damaged parts to
your local Agilent Technologies sales and service office.
CAUTION If there are signs of damage, please do not attempt to install the detector.
Table 3 Detector Checklist
Description Quantity
Detector 1
CompactFlash Card 1 (installed)
Power cable 1
CAN cable 1
Cross-over network cable 1
Twisted pair network cable 1
Flow cell As ordered
User Manual 1
Product CD (includes User and Service Manual) 1
Accessory kit (see Tab l e 4 on page 21 1