Agilent Technologies 1100 Series User manual

A
Agilent 1100 Series
Fluorescence Detector
Reference Manual

2 1100 Series FD Reference Manual
Notices
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2004
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Manual Part Number
G1321-90003
Edition
Edition 05/2004
Printed in Germany
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This guide is valid for A.01.xx revisions of
the Agilent 1100 Series Fluorescence
Detector software, where xx refers to
minor revisions of the software that do
not affect the technical accuracy of this
guide.
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1100 Series FD Reference Manual 3
I This Guide…
1 Installing the Fluorescence Detector
How to install the fluorescence detector
2 First Steps with the Fluorescence Detector
How to start with the detector
3Optimizing the Fluorescence Detector
Detection with the Agilent 1100 series fluorescence detector, either
fluorescence or phosphorescence, can give you very sensitive and
selective results if you understand what you are doing and why. This
chapter explains in detail how to optimize detection.
4Troubleshooting and Test Functions
This chapter describes the detector’s built in troubleshooting and test
functions.
5Repairing the Fluorescence Detector
Instructions on how to repair the detector
6Identifying Parts and Materials
Detailed illustrations and listings for parts and materials identification
for the detector
7Introduction to the Fluorescence Detector
An introduction to the detector
8Therory of Operation

4 1100 Series FD Reference Manual
Instrument overview, theory of operation, external communication and
internal connectors
9Introduction to the Control Module
This chapter is intended to introduce an operator to the screens available
for operation of the Agilent 1100 diode array detector with the Agilent
1100 control module.
Please use the manual of Control module for further detailed reference.
10 Specifications
Performance specifications of the detector

1100 Series FD Reference Manual 5
Contents
1Installing the Fluorescence Detector
Site Requirements 14
Physical Specifications 16
Unpacking the Detector 17
Detector Accessory Kit Contents 18
Optimizing the Stack Configuration 20
Installing the Detector 23
Flow Connections to the Detector 26
2First Steps with the Fluorescence Detector
Before You Start 32
Optimization Overview 33
Getting Started and Checkout 35
Starting Your Detector 35
Setting the Chromatographic Conditions 36
Observe the maxima via the isoabsorbance plot 38
Method Development 39
Step 1: Check the LC system for impurities 40
Step 2: Optimize limits of detection and selectivity 41
Step 3: Set up routine methods 51
Example: Optimization for Multiple Compounds 55

6 1100 Series FD Reference Manual
3Optimizing the Fluorescence Detector
Optimization Overview 64
Design Features Help Optimization 65
Check Performance Before You Start 65
Finding the Best Wavelengths 66
A Real Example 67
Finding the Best Signal Amplification 68
Changing the Xenon Flash Lamp Frequency 70
Lamp life savings 71
Selecting the Best Response Time 72
Reducing Stray Light 74
4Troubleshooting and Test Functions
Oerie of the Detetor’s Idiators ad Test Futios
78
Status Indicators 79
Power Supply Indicator 79
Detector Status Indicator 80
Error Messages 81
Time-out 82
Shutdown 83
Remote Time-out 84
Synchronization Lost 85
Leak 86
Leak Sensor Open 88

1100 Series FD Reference Manual 7
Leak Sensor Short 89
Compensation Sensor
Open
90
Compensation Sensor
Short
91
Fan Failed 92
Motor Errors 93
Lamp Cover Open 95
FLF Board not found 96
ADC Not Calibrated97
A/D Overflow 98
Flash Lamp Current Overflow 99
Flash Trigger Lost 100
Wavelength Calibration Failed 101
Wavelength Calibration Lost 102
Flow Cell Removed 103 No
Peaks 104
Wavelength Verification and Calibration 105
Check of Raman Band of Water 108
The Wavelength Calibration Procedure 114
Preparation of the Glycogen Calibration Sample 114
Preparation of the Flow Cell 115
Wavelength Calibration 116
Verification 117
Test Functions118
Lamp Intensity Test 119
Lamp Intensity History 120

8 1100 Series FD Reference Manual
Dark-Current Test 121
Excitation and Emission Grating Resistance History 122
Raman ASTM Signal-to-Noise Test 123
Using the Built-in Test Chromatogram 126
Using the Built-in DAC Test 129
5Repairing the Fluorescence Detector
Introduction into Repairing the Fluorescence Detector 132
Overview of the Repairing of the Fluorescence Detector
135
Simple Repairs 136
Exchanging a Flow Cell 137
How to use the Cuvette 140
Flow Cell Flushing 141
Correcting Leaks 142
Replacing Leak Handling System Parts 143
Exchanging Internal Parts 144
Removing the Covers 146
Removing the Rear Foam 148
Exchanging the Lamp 149
Exchanging the EX-condenser 154
Replacing the Lamp Cover Window 157
Exchanging the Processor Board 160
Exchanging the Fan 164
Exchanging the Leak Sensor or Leak Panel 166

1100 Series FD Reference Manual 9
Replacing the EM-Condenser or Cutoff-Filter 169
Replacing the Mirror Assembly 172
Replacing the Slits 176
Removing the Optical Unit 178
Replacing PMT and/or FLF board 180
Replacing FLL board and Trigger Pack 183
Replacing the EM and EX Grating 187
Exchanging the Power Supply 190
Replacing Status Light Pipe 193
Installing the Optical Unit 194
Replacing the Foams and Covers 196
Assembling the Main Cover 198
Replacing the Interface Board 199
Replaig the Detetor’s Firare 200
6Identifying Parts and Materials
Overview of Main Assemblies 202
Optical Unit Assembly 204
Lamp Cover Parts 207
Control Module 208
Sheet Metal Kit 209
Plastic Parts 210
Foam Parts 211

10 1100 Series FD Reference Manual
Power and Status Light
Pipes
Leak Parts 213
Cuvette Kit 214
Spare Parts 215
Accessory Kit 216
Cable Overview 218
Analog Cables 220
Remote Cables
223
BCD Cables 230
Auxiliary Cable
232
CAN Cable 233
212
External Contact Cable
RS-232 Cable Kit 235
LAN Cables 236
234
7
Introduction to the Fluorescence
Detector
Introduction to the Detector
238
How the Detector Operates
239
Raman Effect 242
Optical Unit 243
Reference System 250
Analytical Information From Primary Data 251
Fluorescence Detection 251