Agilent Technologies G1314B User manual

Agilent Technologies
Agilent 1260 Infinity
Variable Wavelength
Detector
User Manual

Agilent 1260 Infinity VWD User Manual
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2013
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Manual Part Number
G1314-90013 Rev. C
Edition
11/2013
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Agilent 1260 Infinity VWD User Manual 3
In This Guide
In This Guide
This manual covers the Agilent 1260 Infinity Variable Wavelength Detectors
•G1314B Agilent 1260 Infinity Variable Wavelength Detector VL
•G1314C Agilent 1260 Infinity Variable Wavelength Detector VL+
1 Introduction to the Variable Wavelength Detector
This chapter gives an introduction to the detector, instrument overview
and internal connectors.
2 Site Requirements and Specifications
This chapter gives information on environmental requirements, physical
and performance specifications.
3 Installing the Detector
This chapter provides information on unpacking, checking on
completeness, stack considerations and installation of the module.
4 Using the Detector
This chapter provides information on how to set up the detector for an
analysis and explains the basic settings.
5 How to optimize the detector
This chapter gives hints on how to select the detector parameters and the
flow cell.
6 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.

4 Agilent 1260 Infinity VWD User Manual
In This Guide
7 Error Information
This chapter describes the meaning of detector error messages, and
provides information on probable causes and suggested actions how to
recover from error conditions.
8TestFunctions
This chapter describes the detector’s built in test functions.
9 Maintenance
This chapter provides general information on maintenance and repair of
the detector.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and
electronics.
13 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1260 Infinity VWD User Manual 5
Contents
Contents
1 Introduction to the Variable Wavelength Detector 9
Introduction to the Detector 10
Optical System Overview 11
System Overview 16
2 Site Requirements and Specifications 19
Site Requirements 20
Physical Specifications 24
Performance Specifications 25
3 Installing the Detector 31
Unpacking the Detector 32
Optimizing the Stack Configuration 34
Installation Information on Leak and Waste Handling 39
Installing the Detector 43
Flow Connections to the Detector 46
4 Using the Detector 49
Leak and Waste Handling 50
Setting up an Analysis 51
Special Settings of the Detector 66
5 How to optimize the detector 73
Optimizing the Detector Performance 74
Match the Flow Cell to the Column 75
Set the Detector Parameters 78
6 Troubleshooting and Diagnostics 79
Overview of the Detector’s Indicators and Test Functions 80
Status Indicators 81
Available Tests versus Interfaces 83
Agilent Lab Advisor Software 84

6 Agilent 1260 Infinity VWD User Manual
Contents
7 Error Information 85
What Are Error Messages 86
General Error Messages 87
Detector Error Messages 94
8 Test Functions 103
Intensity Test 104
Cell Test 106
Wavelength Verification-Calibration 108
ASTM Drift and Noise Test 110
Quick Noise Test 111
Dark Current Test 112
Holmium Oxide Test 114
9 Maintenance 117
Introduction to Maintenance 118
Warnings and Cautions 119
Overview of Maintenance 121
Cleaning the Module 122
Exchanging a Lamp 123
Exchanging a Flow Cell 126
Repairing the Flow Cells 128
Using the Cuvette Holder 130
Correcting Leaks 132
Replacing Leak Handling System Parts 133
Replacing the Interface Board 134
Replacing the Module’s Firmware 135

Agilent 1260 Infinity VWD User Manual 7
Contents
10 Parts and Materials for Maintenance 137
Overview of Maintenance Parts 138
Standard Flow Cell 10 mm / 14 µL 139
Micro Flow Cell, 5 mm / 1 µL (only for support) 140
Micro Flow Cell 3 mm / 2 µL 142
Semi-micro Flow Cell 6 mm / 5 µL 144
High Pressure Flow Cell 10 mm / 14 µL 146
Cuvette Holder 148
Leak Parts 149
Kits 150
11 Identifying Cables 151
Cable Overview 152
Analog Cables 154
Remote Cables 156
BCD Cables 159
CAN/LAN Cables 161
RS-232 Cable Kit 162
External Contact Cable 163
12 Hardware Information 165
Firmware Description 166
Optional Interface Boards 169
Electrical Connections 172
Interfaces 175
Setting the 8-bit Configuration Switch (without On-board) LAN 182
Instrument Layout 187
Early Maintenance Feedback (EMF) 188

8 Agilent 1260 Infinity VWD User Manual
Contents
13 Appendix 191
General Safety Information 192
Batteries Information 195
Radio Interference 196
Sound Emission 197
UV Radiation 198
Solvent Information 199
Declaration of Conformity for HOX2 Filter 201
Agilent Technologies on Internet 202

9
Agilent 1260 Infinity VWD User Manual
Agilent Technologies
1
Introduction to the Variable Wavelength
Detector
Introduction to the Detector 10
Optical System Overview 11
Flow Cell 12
G1314B/C Lamp 13
Source Lens Assembly 13
Entrance Slit Assembly 13
Filter Assembly 13
Mirror Assemblies M1 and M2 14
Grating Assembly 14
Beam Splitter Assembly 14
Photo Diodes Assemblies 15
Photo Diode ADC (analog-to-digital converter) 15
System Overview 16
Leak and Waste Handling 16
This chapter gives an introduction to the detector, instrument overview and
internal connectors.

10 Agilent 1260 Infinity VWD User Manual
1Introduction to the Variable Wavelength Detector
Introduction to the Detector
Introduction to the Detector
The Agilent 1260 Infinity variable wavelength detector is designed for
highest optical performance, GLP compliance and easy maintenance with:
•data rate up to , see “Peakwidth Settings” on page 71,
•13 Hz for standard HPLC with G1314B VWD VL,
•55 Hz for fast- HPLC G1314C VWD VL+,
•deuterium lamp for highest intensity and lowest detection limit over a
wavelength range of 190 to 600 nm,
•optional flow- cell cartridges (standard (10 mm, 14 µL), high pressure
(10 mm, 14 µL), micro (5 mm, 1 µL), semi- micro (6 mm, 5 µL)) are
available and can be used depending on the application needs,
•easy front access to lamp and flow cell for fast replacement, and
•built- in holmium oxide filter for fast wavelength accuracy verification.
For specifications refer to Table 4 on page 27.
Two version of the Agilent 1260 Infinity variable wavelength detector are
available:
G1314B Agilent 1260 Infinity Variable Wavelength Detector VL
G1314C Agilent 1260 Infinity Variable Wavelength Detector VL+ high data rates for
fast HPLC