Agilent Technologies G1314B User manual

Agilent Technologies
Agilent 1260 Infinity
Variable Wavelength
Detector
User Manual

Agilent 1260 Infinity VWD User Manual
Notices
© Agilent Technologies, Inc. 2011-2012,
2013
No part of this manual may be reproduced
in any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G1314-90013 Rev. C
Edition
11/2013
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
This product may be used as a com-
ponent of an in vitro diagnostic sys-
tem if the system is registered with
the appropriate authorities and com-
plies with the relevant regulations.
Otherwise, it is intended only for gen-
eral laboratory use.
Warranty
The material contained in this docu-
ment is provided “as is,” and is sub-
ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a par-
ticular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or perfor-
mance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the sep-
arate agreement shall control.
Technology Licenses
The hardware and/or software described in
this document are furnished under a license
and may be used or copied only in accor-
dance with the terms of such license.
Restricted Rights Legend
If software is for use in the performance of a
U.S. Government prime contract or subcon-
tract, Software is delivered and licensed as
“Commercial computer software” as
defined in DFAR 252.227-7014 (June 1995),
or as a “commercial item” as defined in FAR
2.101(a) or as “Restricted computer soft-
ware” as defined in FAR 52.227-19 (June
1987) or any equivalent agency regulation
or contract clause. Use, duplication or dis-
closure of Software is subject to Agilent
Technologies’ standard commercial license
terms, and non-DOD Departments and
Agencies of the U.S. Government will
receive no greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will receive
no greater than Limited Rights as defined in
FAR 52.227-14 (June 1987) or DFAR
252.227-7015 (b)(2) (November 1995), as
applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully under-
stood and met.

Agilent 1260 Infinity VWD User Manual 3
In This Guide
In This Guide
This manual covers the Agilent 1260 Infinity Variable Wavelength Detectors
•G1314B Agilent 1260 Infinity Variable Wavelength Detector VL
•G1314C Agilent 1260 Infinity Variable Wavelength Detector VL+
1 Introduction to the Variable Wavelength Detector
This chapter gives an introduction to the detector, instrument overview
and internal connectors.
2 Site Requirements and Specifications
This chapter gives information on environmental requirements, physical
and performance specifications.
3 Installing the Detector
This chapter provides information on unpacking, checking on
completeness, stack considerations and installation of the module.
4 Using the Detector
This chapter provides information on how to set up the detector for an
analysis and explains the basic settings.
5 How to optimize the detector
This chapter gives hints on how to select the detector parameters and the
flow cell.
6 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.

4 Agilent 1260 Infinity VWD User Manual
In This Guide
7 Error Information
This chapter describes the meaning of detector error messages, and
provides information on probable causes and suggested actions how to
recover from error conditions.
8TestFunctions
This chapter describes the detector’s built in test functions.
9 Maintenance
This chapter provides general information on maintenance and repair of
the detector.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and
electronics.
13 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1260 Infinity VWD User Manual 5
Contents
Contents
1 Introduction to the Variable Wavelength Detector 9
Introduction to the Detector 10
Optical System Overview 11
System Overview 16
2 Site Requirements and Specifications 19
Site Requirements 20
Physical Specifications 24
Performance Specifications 25
3 Installing the Detector 31
Unpacking the Detector 32
Optimizing the Stack Configuration 34
Installation Information on Leak and Waste Handling 39
Installing the Detector 43
Flow Connections to the Detector 46
4 Using the Detector 49
Leak and Waste Handling 50
Setting up an Analysis 51
Special Settings of the Detector 66
5 How to optimize the detector 73
Optimizing the Detector Performance 74
Match the Flow Cell to the Column 75
Set the Detector Parameters 78
6 Troubleshooting and Diagnostics 79
Overview of the Detector’s Indicators and Test Functions 80
Status Indicators 81
Available Tests versus Interfaces 83
Agilent Lab Advisor Software 84

6 Agilent 1260 Infinity VWD User Manual
Contents
7 Error Information 85
What Are Error Messages 86
General Error Messages 87
Detector Error Messages 94
8 Test Functions 103
Intensity Test 104
Cell Test 106
Wavelength Verification-Calibration 108
ASTM Drift and Noise Test 110
Quick Noise Test 111
Dark Current Test 112
Holmium Oxide Test 114
9 Maintenance 117
Introduction to Maintenance 118
Warnings and Cautions 119
Overview of Maintenance 121
Cleaning the Module 122
Exchanging a Lamp 123
Exchanging a Flow Cell 126
Repairing the Flow Cells 128
Using the Cuvette Holder 130
Correcting Leaks 132
Replacing Leak Handling System Parts 133
Replacing the Interface Board 134
Replacing the Module’s Firmware 135

Agilent 1260 Infinity VWD User Manual 7
Contents
10 Parts and Materials for Maintenance 137
Overview of Maintenance Parts 138
Standard Flow Cell 10 mm / 14 µL 139
Micro Flow Cell, 5 mm / 1 µL (only for support) 140
Micro Flow Cell 3 mm / 2 µL 142
Semi-micro Flow Cell 6 mm / 5 µL 144
High Pressure Flow Cell 10 mm / 14 µL 146
Cuvette Holder 148
Leak Parts 149
Kits 150
11 Identifying Cables 151
Cable Overview 152
Analog Cables 154
Remote Cables 156
BCD Cables 159
CAN/LAN Cables 161
RS-232 Cable Kit 162
External Contact Cable 163
12 Hardware Information 165
Firmware Description 166
Optional Interface Boards 169
Electrical Connections 172
Interfaces 175
Setting the 8-bit Configuration Switch (without On-board) LAN 182
Instrument Layout 187
Early Maintenance Feedback (EMF) 188

8 Agilent 1260 Infinity VWD User Manual
Contents
13 Appendix 191
General Safety Information 192
Batteries Information 195
Radio Interference 196
Sound Emission 197
UV Radiation 198
Solvent Information 199
Declaration of Conformity for HOX2 Filter 201
Agilent Technologies on Internet 202

9
Agilent 1260 Infinity VWD User Manual
Agilent Technologies
1
Introduction to the Variable Wavelength
Detector
Introduction to the Detector 10
Optical System Overview 11
Flow Cell 12
G1314B/C Lamp 13
Source Lens Assembly 13
Entrance Slit Assembly 13
Filter Assembly 13
Mirror Assemblies M1 and M2 14
Grating Assembly 14
Beam Splitter Assembly 14
Photo Diodes Assemblies 15
Photo Diode ADC (analog-to-digital converter) 15
System Overview 16
Leak and Waste Handling 16
This chapter gives an introduction to the detector, instrument overview and
internal connectors.

10 Agilent 1260 Infinity VWD User Manual
1Introduction to the Variable Wavelength Detector
Introduction to the Detector
Introduction to the Detector
The Agilent 1260 Infinity variable wavelength detector is designed for
highest optical performance, GLP compliance and easy maintenance with:
•data rate up to , see “Peakwidth Settings” on page 71,
•13 Hz for standard HPLC with G1314B VWD VL,
•55 Hz for fast- HPLC G1314C VWD VL+,
•deuterium lamp for highest intensity and lowest detection limit over a
wavelength range of 190 to 600 nm,
•optional flow- cell cartridges (standard (10 mm, 14 µL), high pressure
(10 mm, 14 µL), micro (5 mm, 1 µL), semi- micro (6 mm, 5 µL)) are
available and can be used depending on the application needs,
•easy front access to lamp and flow cell for fast replacement, and
•built- in holmium oxide filter for fast wavelength accuracy verification.
For specifications refer to Table 4 on page 27.
Two version of the Agilent 1260 Infinity variable wavelength detector are
available:
G1314B Agilent 1260 Infinity Variable Wavelength Detector VL
G1314C Agilent 1260 Infinity Variable Wavelength Detector VL+ high data rates for
fast HPLC

Agilent 1260 Infinity VWD User Manual 11
Introduction to the Variable Wavelength Detector 1
Optical System Overview
Optical System Overview
The optical system of the detector is shown in the figure below. Its
radiation source is a deuterium- arc discharge lamp for the ultraviolet (UV)
wavelength range from 190 to 600 nm. The light beam from the deuterium
lamp passes through a lens, a filter assembly, an entrance slit, a spherical
mirror (M1), a grating, a second spherical mirror (M2), a beam splitter,
and finally through a flow cell to the sample diode. The beam through the
flow cell is absorbed depending on the solutions in the cell, in which UV
absorption takes place, and the intensity is converted to an electrical
signal by means of the sample photodiode. Part of the light is directed to
the reference photodiode by the beam splitter to obtain a reference signal
for compensation of intensity fluctuation of the light source. A slit in front
of the reference photodiode cuts out light of the sample bandwidth.
Wavelength selection is made by rotating the grating, which is driven
directly by a stepper motor. This configuration allows fast change of the
wavelength. The cutoff filter is moved into the lightpath above 370 nm to
reduce higher order light.
Figure 1 Optical Path of the Variable Wavelength Detector
9ZjiZg^jbaVbe
;^aiZgVhhZbWan
AZch
:cigVcXZha^i
B^ggdgB&
B^ggdgB'
<gVi^c\
GZ[ZgZcXZY^dYZ
HVbeaZY^dYZ
;adlXZaa
7ZVbhea^iiZg

12 Agilent 1260 Infinity VWD User Manual
1Introduction to the Variable Wavelength Detector
Optical System Overview
Flow Cell
A variety of flow- cell cartridges can be inserted using the same quick and
simple mounting system.
Figure 2 Cartridge Type Flow Cell
Table 1 Flow Cell Data
STD Semi-micro Micro High Pressure
Maximum pressure 40 (4) 40 (4) 120 (12) 400 (40) bar
Path length 10 (conical) 6 (conical) 3 (conical) 10 (conical) mm
Volume 14 5 2 14 µL
Inlet i.d. 0.25 0.17 0.12 0.25 mm
Inlet length 750 250 310 750 mm
Outlet i.d. 0.30 0.17 0.17 0.17 mm
Outlet length 120 120 120 120 mm
Total volume 60.77 14.49 14.00 60.77 µL
Materials in contact
with solvent
SST, quartz, PTFE,
PEEK
SST, quartz, PTFE SST, quartz, PTFE SST, quartz, Kapton

Agilent 1260 Infinity VWD User Manual 13
Introduction to the Variable Wavelength Detector 1
Optical System Overview
G1314B/C Lamp
The light source for the UV wavelength range is a deuterium lamp. As a
result of plasma discharge in a low pressure deuterium gas, the lamp
emits light over the 190 to 600 nm wavelength range.
Source Lens Assembly
The source lens receives the light from the deuterium lamp and focuses it
onto the entrance slit.
Entrance Slit Assembly
The entrance slit assembly has an exchangeable slit. The standard one has
a 1- mm slit. For replacement and calibration purposes to optimize the
alignment, a slit with a hole is needed.
Filter Assembly
The filter assembly is electromechanically actuated. During wavelength
calibrations it moves into the light path.
The filter assembly has two filters installed and is processor- controlled.
A photo sensor determines the correct position.
OPEN nothing in light path
CUTOFF cut off filter in light path at λ> 370 nm
HOLMIUM holmium oxide filter for wavelength check

14 Agilent 1260 Infinity VWD User Manual
1Introduction to the Variable Wavelength Detector
Optical System Overview
Figure 3 Filter Assembly
Mirror Assemblies M1 and M2
The instrument contains two spherical mirrors (M1 and M2). The beam
adjustable is vertically and horizontally. Both mirrors are identical.
Grating Assembly
The grating separates the light beam into all its component wavelengths
and reflects the light onto mirror #2.
Beam Splitter Assembly
The beam splitter splits the light beam. One part goes directly to the
sample diode. The other part of the light beam goes to the reference
diode.
8jid[[[^aiZg
=dab^jbdm^YZ[^aiZg
l^i]Xjid[[[^aiZg

Agilent 1260 Infinity VWD User Manual 15
Introduction to the Variable Wavelength Detector 1
Optical System Overview
Photo Diodes Assemblies
Two photo diode assemblies are installed in the optical unit. The sample
diode assembly is located on the left side of the optical unit. The
reference diode assembly is located in the front of the optical unit.
Photo Diode ADC (analog-to-digital converter)
The photo diode current is directly converted to digital data direct photo
current digitalization. The data is transferred to the detector main board .
The photo diode ADC boards are located close to the photo diodes.

16 Agilent 1260 Infinity VWD User Manual
1Introduction to the Variable Wavelength Detector
System Overview
System Overview
Leak and Waste Handling
The 1200 Infinity Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.

Agilent 1260 Infinity VWD User Manual 17
Introduction to the Variable Wavelength Detector 1
System Overview
Figure 4 Leak and waste handling concept (overview - typical stack configuration as an
example)
+
6
7
8
,
,
*
)
(
'
&

18 Agilent 1260 Infinity VWD User Manual
1Introduction to the Variable Wavelength Detector
System Overview
The solvent cabinet (1) is designed to store a maximum volume of 6 L
solvent. The maximum volume for an individual bottle stored in the
solvent cabinet should not exceed 2.5 L. For details, see the usage
guideline for the Agilent 1200 Infinity Series Solvent Cabinets (a printed
copy of the guideline has been shipped with the solvent cabinet, electronic
copies are available on the Internet).
The leak pan (2) (individually designed in each module) guides solvents to
the front of the module. The concept covers also leakages on internal
parts (e.g. the detector’s flow cell). The leak sensor in the leak pan stops
the running system as soon as the leak detection level is reached.
The leak pan's outlet port (3, A) guides excessive overfill from one module
to the next, as the solvent flows into the next module’s leak funnel (3, B)
and the connected corrugated waste tube (3, C). The corrugated waste
tube guides the solvent to the next lower positioned module’s leak tray
and sensor.
The waste tube of the sampler’s needle wash port (4) guides solvents to
waste.
The condense drain outlet of the autosampler cooler (5) guides condensate
to waste.
The waste tube of the purge valve (6) guides solvents to waste.
The waste tube connected to the leak pan outlet on each of the bottom
instruments (7) guides the solvent to a suitable waste container.

19
Agilent 1260 Infinity VWD User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 20
Physical Specifications 24
Performance Specifications 25
Specification Conditions 29
This chapter gives information on environmental requirements, physical and
performance specifications.

20 Agilent 1260 Infinity VWD User Manual
2Site Requirements and Specifications
Site Requirements
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Consideration
The detector power supply has wide ranging capabilities, see “Physical
Specifications” on page 24. It accepts any line voltage in the above
mentioned range. Consequently, there is no voltage selector in the rear of
the detector. There are also no externally accessible fuses, because
automatic electronic fuses are implemented in the power supply.
WARNING
Instrument is partially energized when switched off
The power supply still uses some power, even when the power switch on the front
panel is turned OFF. Repair work at the detector can lead to personal injuries, e. g.
shock hazard, when the detector cover is opened and the instrument is connected to
power.
➔To disconnect the detector from the power line, unplug the power cord.
WARNING
Hazard of electrical shock or damage of your instrumentation
can result, if the devices are connected to a line voltage higher than specified.
➔Connect your instrument to the specified line voltage only.