Agilent Technologies 8453 Wiring diagram

Agilent Technologies
Agilent 8453 Dissolution
Testing System
Installing and Operating
Manual

Agilent 8453 Dissolution Testing System Installing and Operating Manual
Notices
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Manual Part Number
G1127-90003
Edition
09/2008
Printed in Germany
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met.

Agilent 8453 Dissolution Testing System Installing and Operating Manual 3
Introduction
This section introduces you to Agilent dissolution testing software and
explains how to use this handbook to install the hardware and software.
What is Dissolution Testing?
The dissolution testing software for the Agilent 8453 UV-visible
spectrophotometer and Agilent ChemStation automates totally the process of
measuring the rates of release of active ingredients from formulations into
solutions. Your dissolution testing software is provided on CD-ROM to install
and operate in the graphic environment of the supported Microsoft Windows
operating system(s). For details see the Specifications for your Agilent
ChemStation for UV-visible Spectroscopy Systems. The software automates
the sampling, measuring, data processing, and reporting of the dissolution
test. The dissolution testing software requires that you develop your own
specific testing method for either single-component or multicomponent
analysis. The software provides all the necessary features for developing a test
method.
The dissolution testing software samples and measures your samples
automatically in the Agilent 8453 UV-visible spectrophotometer. The software
compares the absorbance measurements with previously measured known
standards. The software calculates the concentration of component in your
samples and calculates dissolution results automatically.
You choose in your test method the dissolution results that you want reported.
The dissolution testing software calculates the following choices of dissolution
results for the ingredient of interest:
• % Dissolved or released
• Weight dissolved or released per tablet
• Weight dissolved or released per weight of tablet
These results are based on label weight or on tablet weight.

4 Agilent 8453 Dissolution Testing System Installing and Operating Manual
What To Do
You use this handbook to set up your dissolution testing system. This is what
you do:
• follow the instructions in this handbook,
• refer to other handbooks when necessary, then
• return to the next step in this handbook for completion of setting up.
What You Need
Your dissolution testing system comprises the following components:
See your packing check list for your system options. You use the system with
your dissolution vessels and bath.
What You Install
• Agilent 8453 UV-visible spectroscopy system
• Dissolution sampling system
The chapters that follow describe the installation procedures for dissolution
sampling systems.
Spectrophotometer Agilent 8453 UV-visible spectrophotometer.
Sample Introduction System 8-port valve and single channel pump, multicell transport with
multichannel pump, or multicell transport with eight-port valve and
two single-channel pumps.
Personal Computer PC with Microsoft Windows operating system and printer.
Application Software Agilent ChemStation general purpose software and dissolution
testing software for UV-visible spectroscopy.

Agilent 8453 Dissolution Testing System Installing and Operating Manual 5
In This Guide…
This handbook provides you with instructions for installing your Agilent
dissolution testing system.
1 Site Requirements and Specifications
This chapter describes the site requirements and contains the specifications
for all units applied.
2SettingUp
This chapter describes how to set up your Agilent 8453 UV-visible
spectroscopy system and dissolution sampling system.
3 Using the Peristaltic Pumps 1VS and 8VS
This chapter describes how to install, operate, maintain and service the
peristaltic pumps.
4 Using the Valve Unit and Valve-pump Controller
This chapter describes how to install, operate, maintain and service the valve
unit and valve-pump controller.
5 Parts for Dissolution Testing
This chapter contains lists of parts and their numbers you need for the
different configurations of dissolution testing systems.
A Safety Information
This chapter contains relevant safety information.

6 Agilent 8453 Dissolution Testing System Installing and Operating Manual

Agilent 8453 Dissolution Testing System Installing and Operating Manual 7
Contents
1 Site Requirements and Specifications 11
Site Requirements 12
Site Requirements 12
General Considerations 12
Power Consideration 12
Power Cords 13
Bench Space 13
Environment 14
Specifications 15
Peristaltic Pumps 1VS and 8VS 15
Valve Pump Controller 17
Valve unit 19
2SettingUp21
Setting Up Your Spectrophotometer 22
Setting Up Your Sampling System 23
Naming Parts 23
Dissolution Bath 26
Installing a Multicell-transport-based Sampling System 27
Installing Your Eight-position Multicell Transport 28
Installing Your Peristaltic Pump 8VS 28
Connecting Multicell System Tubing 31
Troubleshooting 34
Part Numbers 35
Installing a Valve-based Sampling System 36
Setting Up Your Valve-pump Controller 38

8 Agilent 8453 Dissolution Testing System Installing and Operating Manual
Contents
Setting Up Your Valve Unit 42
Connecting Cables 44
Installing Your Peristaltic Pump 1VS 46
Connecting Valve System Tubing 49
Replacing Tubing 50
Troubleshooting 51
Part Numbers 52
Installing Your Multibath System 53
Installing Your Multicell Transport 54
Setting Up Your Valve-pump Controller 55
Setting Up Your Valve Units 58
Connecting Cables 60
Installing Your Peristaltic Pump 1VS 62
Connecting Valve System Tubing 65
Replacing Tubing 66
Troubleshooting 67
Part Numbers 68
3 Using the Peristaltic Pumps 1VS and 8VS 69
Installing Your Pump 70
Viewing the Pump 70
Operating Your Pump 73
Adjusting Flow Rate 73
Maintaining Your Pump 74
Removing the Tubing Cassette 75
Changing the Pressure-wheel Assembly 75
Changing the Drive Belt 77
Changing the Fuse 79
Servicing Your Pump 80
Electronics Description 80
Checking Remote Operation 82

Contents
Agilent 8453 Dissolution Testing System Installing and Operating Manual 9
4 Using the Valve Unit and Valve-pump Controller 85
Operating Your Valve System 86
Operating Your Valve Unit 86
Operating Your Pump 87
Troubleshooting Your Valve System 88
Checking the Problem 88
Solving Your Problem 91
Part Numbers 96
Servicing Your Valve System 98
Electronics Overview 99
Troubleshooting 106
Cables and Connectors 110
5 Parts for Dissolution Testing 113
Parts for Dissolution Testing 114
A Safety Information 119
Safety Information 120
General 120
Operation 120
Safety Symbols 121
Agilent Technologies on Internet 123
Index 125

10 Agilent 8453 Dissolution Testing System Installing and Operating Manual
Contents

11
Agilent 8453 Dissolution Testing System
Installing and Operating Manual
Agilent Technologies
1
Site Requirements and Specifications
Site Requirements 12
Specifications 15

12 Agilent 8453 Dissolution Testing System Installing and Operating Manual
1 Site Requirements and Specifications
Site Requirements
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
entire system for automated dissolution testing. The components are designed
for indoor use only in a clean lab environment.
All components required for your system must be available. For the
dissolution testing equipment refer to the supplier’s documentation.
General Considerations
Power Consideration
The peristaltic pumps 1FS, 1VS and 8VS and the valve-pump controller are
available in two versions for line voltages of 100 - 120 V AC or 220 - 240 V AC.
Before you connect your device to a power outlet, make sure your device
version is in coincidence with your line voltage supply.
NOTE
Make sure your Agilent spectroscopy system is installed and qualified before you add your
dissolution sampling system.
CAUTION
Only use the equipment as described and specified in this handbook. If used in a
manner not specified by Agilent, protection of the equipment may be impaired.

Agilent 8453 Dissolution Testing System Installing and Operating Manual 13
Site Requirements and Specifications 1
Site Requirements
Power Cords
Different power cords are offered as options with the sampling system
components. The female end of the power cords is identical. It plugs into the
power-input socket at the rear of the different peristaltic pumps and the
valve-pump controller. The male end of each power cord is different and
designed to match the wall socket of a particular country or region.
Bench Space
The sampling system is attached to a dissolution tester and the 8453
spectrophotometer. Agilent recommends a linear arrangement on a lab bench
of the dissolution tester, the sampling devices, the 8453 spectrophotometer
and the PC.
For the optimum arrangement the tubing connecting the vessel probe or valve
outlet to the flow cell inlet should be as short as possible. In case of a single
bath system, the 8453 should be adjacent to the dissolution tester. In a
multibath environment, the 8453 should be in the center of the arrangement.
The sampling devices applied should be visible to facilitate a check for proper
operation.
WARNING
The absence of ground connection and the use of an unspecified power cord can
lead to electric shock or short circuit.
Electric Shock
•Never operate your instrumentation from a power outlet that has no ground
connection.
•Never use a power cord other than the Agilent Technologies power cord
designed for your region.
WARNING
Use of non Agilent cables
The use of cables which haven't been supplied by Agilent Technologies can lead to
damage of the electronic components or personal injury.
•Never use cables other than the ones supplied by Agilent Technologies to
ensure proper functionality and compliance with safety or EMC regulations.

14 Agilent 8453 Dissolution Testing System Installing and Operating Manual
1 Site Requirements and Specifications
Site Requirements
Environment
Your system will work as specified individually for each of the components.
The operation limits here are defined by the weakest specification of all
applied devices.
Specifications for the Agilent devices are with the respective manuals. For the
sampling devices please check the respective specifications later in this
chapter.
NOTE
Make sure the bench is designed to carry the weight of all components of your automated
dissolution testing system.
CAUTION
Condensation conditions must be avoided. The electronics of the devices may be damaged
if condensation occurs.

Agilent 8453 Dissolution Testing System Installing and Operating Manual 15
Site Requirements and Specifications 1
Specifications
Specifications
Peristaltic Pumps 1VS and 8VS
The peristaltic pumps 1VS and 8VS are for one and eight pumping channels.
The pumps are designed for liquid samples. Due to the peristaltic pump
principle only the tubing is in contact with the liquid.
The pumps can be operated manually and remotely via a digital interface. If
used for automated sampling in the dissolution testing application, only the
remote operation is applied.
NOTE
Connect the pump 1VS to the valve-pump-controller only by the controller-to-pump cable
(PN G1103-61612) provided by Agilent.
Connect the pump 8VS to the 8453 spectrophotometer only by the GPIO-to-pump cable
(PN G1103-61607) provided by Agilent
Table 1 Physical Specifications Peristaltic Pumps 1VS and 8VS
Type Specification Comments
Dimensions 15.9 cm (6.2 inches) wide
19.3 cm (7.6 inches) deep
11.2 cm (4.4 inches) high
15.9 cm (6.2 inches) wide
26.5 cm (10.5 inches) deep
11.2 cm (4.4 inches) high
Pump 1VS
Pump 8VS
Weight 3.25 kg (7.2 lbs)
3.3 kg (7.3 lbs)
Pump 1VS
Pump 8VS
Line voltage 100–120 V AC ± 10%
220–240 V AC ± 10%
Select 115 V AC
Select 230 V AC
Line frequency 50–60 Hz
Power consumption 55 VA Maximum
Ambient operating temperature 5–40 °C (41–104 °F)

16 Agilent 8453 Dissolution Testing System Installing and Operating Manual
1 Site Requirements and Specifications
Specifications
Ambient non-operating temperature -40–70 °C (-4–158 °F)
Humidity <90%, at 25–40 °C (77–104 °F) Non-condensing
Operating altitude Up to 2000 m (6,500 ft)
Non-operating altitude Up to 4600 m (14,950 ft) For storing the
instrument
Safety standards: IEC, CSA, UL Installation Category II, Pollution
Degree 2
For indoor use only
Table 2 Performance Specifications Peristaltic Pumps 1VS and 8VS
Type Specification Comments
Flow rate 2 - 14 ml/min Pump tubing with 2.06 mm inner diameter
Speed 1 - 67 rpm clock wise and counter clockwise operation
Pumping pressure < 2 bar (30 psi)
Pumping distance < 7 m
Table 1 Physical Specifications (continued)Peristaltic Pumps 1VS and 8VS
Type Specification Comments

Agilent 8453 Dissolution Testing System Installing and Operating Manual 17
Site Requirements and Specifications 1
Specifications
Valve Pump Controller
The valve pump controller is used with all dissolution sampling systems based
on stream selecting valves. The sampling systems are referred as valve
sampling systems if a single dissolution tester is combined with the
spectrophotometer or a multibath sampling system if more than one
dissolution tester is hooked to the instrument.
A maximum of four valve units and four peristaltic pumps 1VS can be
controlled by the valve pump controller.
NOTE
Connect the valve pump controller to the 8453 spectrophotometer only by the
GPIO-to-controller cable (PN G1103-61610) provided by Agilent.
Table 3 Physical Specifications Valve Pump Controller
Type Specification Comments
Dimensions 16.0 cm (6.3 inches) wide
18.0 cm (7.1 inches) deep
11.0 cm (4.3 inches) high
without connected
cables
Weight 2.4 kg (5.3 lbs)
Line voltage 100–120 V AC ± 10%
220–240 V AC ± 10%
Select 115 V AC
Select 230 V AC
Line frequency 50–60 Hz ± 5%
Power consumption 55 VA Maximum
Ambient operating temperature 5–40 °C (41–104 °F)
Ambient non-operating temperature -40–70 °C (-4–158 °F)
Humidity <90%, at 25–40 °C (77–104 °F) Non-condensing
Operating altitude Up to 2000 m (6,500 ft)
Non-operating altitude Up to 4600 m (14,950 ft) For storing the
instrument
Safety standards: IEC, CSA, UL Installation Category II, Pollution
Degree 2
For indoor use only

18 Agilent 8453 Dissolution Testing System Installing and Operating Manual
1 Site Requirements and Specifications
Specifications
Table 4 Performance Specifications Valve Pump Controller
Type Specification Comments
Interface to
spectrophotometer
D-sub, 25-pin female connector 8 bit digital input/output
TTL level
Interface to pumps
(4 connectors)
D-sub, 9-pin female connector 2 bit digital output
TTL level
Interface to valve units
(4 connectors)
D-sub, 15-pin female connector 8 bit digital input/output
TTL level
5 V DC power supply
24 V AC power supply

Agilent 8453 Dissolution Testing System Installing and Operating Manual 19
Site Requirements and Specifications 1
Specifications
Valve unit
The valve unit gets its power from the valve-pump controller. Up to four valve
units can be connected to a single valve pump controller.
NOTE
Connect the valve unit to the valve-pump controller only by the controller-to-valve cable
(PN 5062-2407) provided by Agilent.
Table 5 Physical Specifications Valve Unit
Type Specification Comments
Dimensions 15.9 cm (6.2 inches) wide
26.5 cm (10.5 inches) deep
11.2 cm (4.4 inches) high
Without tubing connected
Weight 3 kg (6.6 lbs)
Power Supply 24 V AC ± 10% provided by Valve-Pump Controller
Line frequency 50 or 60 Hz Line frequency of Valve-Pump Controller
Ambient operating
temperature
5–40 °C (41–104 °F)
Ambient non-operating
temperature
-40–70 °C (-4–158 °F)
Humidity <90%, at 25–40 °C (77–104
°F)
Non-condensing
Operating altitude Up to 2000 m (6,500 ft)
Non-operating altitude Up to 4600 m (14,950 ft) For storing the instrument
Ambient non-operating
temperature
-40–70 °C (-4–158 °F)
Savety considerations Pollution degree 2 For indoor use only
Table 6 Performance Specifications Valve Unit
Type Specification Comments
Switching speed < 500 ms from port to adjacent port

20 Agilent 8453 Dissolution Testing System Installing and Operating Manual
1 Site Requirements and Specifications
Specifications
Valve stator PPS
Valve rotor TFE composite
Temperature range of
solvent
5–75 °C (41–167 °F)
Pressure Less than or equal to ambient
pressure
Pressure drop across
valve
< 0.2 bar (2.9 psi) at 5 ml/min
Flow rate through valve Typically 10 ml/min
Solvents Compatible with PPS and TFE
composite materials, pH 1–14
Lifetime Typically 100,000 switching cycles Pumping water at 20 °C (68 °F)
through each port during
switching. A switching cycle is a
360° turn of the valve. Depending
on your application, you may
need to clean the valve at regular
intervals.
Table 6 Performance Specifications Valve Unit