Antec Scientific ROXY Exceed User manual

T [phone] | [email protected] | www.AntecScientific.com
Antec Scientific
Industrieweg 12
2382 NV Zoeterwoude
The Netherlands
ROXY Exceed
User Manual
211.0010, Edition 4, 2020

2
Copyright ©2020, Antec, The Netherlands. Contents of this publication may not be reproduced in any form or by any means
(including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent
from the copyright of the owner. Antec reserves the right to make changes to the design and specifications of the instrument
and this manual without prior notice.
DECADE Elite, DECADE Lite, ROXY, ALEXYS, ROXY Exceed, INTRO, FlexCell, ReactorCell, µ-PrepCell, ISAAC, HyREF,
SenCell and SynthesisCell are trademarks of Antec Scientific. Whatman™ (word and device) and Whatman™ (word only) are
trademarks of Whatman international Ltd. SOLVENT IFD™ and AQUEOUS IFD™ are trademarks of Arbor Technologies, Inc.
Clarity®, DataApex® are trademarks of DataApex Ltd. Microsoft®and Windows™ are trademarks of Microsoft Corporation. Ex-
cel is a registered trademark of the Microsoft Corporation. ChromeleonTM is a trademark of ThermoFisher Scientific. All other
trademarks are the property of their respective owners.
The software and the information provided herein is believed to be reliable. Antec shall not be liable for errors contained
herein or for incidental or consequential damages in connection with the furnishing, performance, or use of software or this
manual. All use of the software shall be entirely at the user’s own risk.

Declaration of Conformity
We Antec Leyden B.V., Zoeterwoude, The Netherlands, declare that the product:
ROXY Exceed ™ Potentiostat type 211
to which this declaration relates, is in conformity with the following CE directives:
2014/35/EU Low Voltage Directive (LVD)
2014/30/EU Electromagnetic Compatibility Directive (EMC)
2015/863/EU Directive on Restriction of the use of certain Hazardous Substances (RoHS)
2012/19/EU Directive on Waste Electrical and Electronic Equipment (WEEE)
Product standards used for demonstration of compliance:
EN 61010-1:2010
Safety requirements for electrical equipment for measurement, control, and laboratory use; part 1:
General Requirements.
EN 61010-2-010:2014
Safety requirements for electrical equipment for measurement, control, and laboratory use; part 2-
010: Particular requirements for laboratory equipment for the heating of materials.
EN 61326-1:2013
Electrical equipment for measurement, control and laboratory use - EMC requirements; part 1: Gen-
eral Requirements (Class B equipment).
EN 55032:2015
Electromagnetic compatibility of multimedia equipment
Emission requirements (Class B equipment).
Only use manufacturer-supplied cable(s) to connect with other devices. Thoroughly connect
shielding to common. Manufacturer will not accept any liability for damage, direct or indirect,
caused by connecting this instrument to devices and with cables which do not meet relevant
safety standards. All cables used should not exceed a length of 3 meters.
This DoC applies to above-listed product placed on the market after:
Date: January 31th, 2020
Dr. N.J. Reinhoud (managing Director)

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Table of contents
Symbols 7
Intended use 9
WEEE directive 10
ROHS directive 10
Safety instructions 11
Introduction 15
Instrument description 16
Installation 19
Storage requirements 19
Site Preparation Requirements 19
Unpacking 24
Mains connection 26
PC connection 26
Software 30
LC connections 31
Maintenance & Shutdown 35
Maintenance 35
Shutting down the system 37
ROXY Exceed control 39
Introduction 39
Overview of ROXY Exceed screens 40
Parameters 45
Dialogue Elite 50
Chromeleon CDS 52
Operation modes & Parameters 53
Three- or two electrode configuration 53
Internal organization 55
Dual flow cell control 55
Parameters 57
Filter 60
Measurement modes 64
DC mode 64
Pulse mode 65
Pulse mode -2 67
Scan mode 68
Optimization of working potential 69
Hydrodynamic and scanning voltammogram 71
Specifications ROXY Exceed 85
Rear panel I/O 93
USB B connector 94
LAN connector 94
VALVE connector 94
ANALOG DATA connector 100

CHAPTER 1 Table of contents 5
Digital I/O connector 103
Chassis grounding stud 110
Troubleshooting 113
Instrument errors 113
Dummy cell test 115
Potentiostat accessories 121
Index 124

6 211_0010 - ROXY Exceed user manual - rev 04

CHAPTER 1 Table of contents 7
Symbols
The following symbols might be used in this guide or may be found on the
instrument:
This sign warns about the risk of electric shock. It calls
attention to a procedure or practice which, if not adhered
to, could result in loss of life by electrocution. Do not pro-
ceed beyond a danger sign until the indicated conditions
are fully understood and met.
The warning sign denotes a hazard. It calls attention to a
procedure or practice which, if not adhered to, could re-
sult in severe injury, loss of life or damage or destruction
of parts or all of the equipment. Do not proceed beyond a
warning sign until the indicated conditions are fully under-
stood and met.
The caution sign denotes a hazard. It calls attention to a
procedure or practice which, if not adhered to, could re-
sult in damage or destruction of parts or all of the equip-
ment and/or erratic results. Do not proceed beyond a
cautions sign until the indicated conditions are fully un-
derstood and met.
The biohazard sign draws attention to the fact that use
of biological materials, viral samples may carry a signifi-
cant health risk.
The toxic hazard sign draws attention to the fact that use
of toxic solvents or samples may carry a significant
health risk.

8 211_0010 - ROXY Exceed user manual - rev 04
The hot surface sign calls attention to parts in the
instrument that must not be touched, as they may cause
burns.
This symbol indicates electrostatic discharge (ECD haz-
ard), damages to system, device, or components can oc-
cur if not properly grounded.
This symbol indicates that the waste of electrical and
electronic equipment must not be disposed as unsorted
municipal waste and must be collected separately.
Please contact the manufacturer or authorized repre-
sentative of the manufacturer for information concerning
the decommissioning of equipment.
A device or system marked with CE fulfills the product
specific requirements described in the European direc-
tives. This is confirmed in a Declaration of Conformity.
Certification marking for Canada and the USA issued by
MET labs, a Nationally Recognized Testing Laboratory
(NRTL). The certified device complies to the relevant UL
and CSA safety (61010-1) standards.
Frame or chassis ground terminal, which can be used as
to make additional external grounding connection.
The note sign signals additional information. It provides
advice or a suggestion that may support you in using the
equipment.
The attention sign signals relevant information. Read this
information.

CHAPTER 1 Table of contents 9
Intended use
The ROXY potentiostat in combination with flow-through reactor (µ-
PrepCell or ReactorCell) or batch reactor cells (SynthesisCell) is used for
controlled REDOX reactions up-front Mass Spectrometric detection. It can
be used in a wide range of applications, for example:
•Fast synthesis of metabolites (µ-preparative)
•Rapid risk assessments of drug-protein binding
•Signal enhancement in MS
•Electrochemical cleavage of proteins/peptides
•Reduction of disulfide bonds in proteins/peptides
•Oxidative stress/damage of proteins, DNA, lipids, etc.
This instrument is not specifically designed and intended for quantification
of analytes in LC, for that purpose the DECADE Elite/Lite electrochemical
detector is available. Operation of the ROXY potentiostat in combination
with a flow cell can involve the use of hazardous materials including corro-
sive fluids and flammable liquids. The instrumentation should only be oper-
ated by users with the following expertise:
•Completed degree as chemical laboratory technician or
comparable vocational training
•Fundamental knowledge of liquid chromatography equipment and
mass spectrometry
•Participation in an installation of the system performed by the
manufacturer or a company authorized by the manufacturer and
suitable training on the system and chromatography software.
•Knowledge and experience in the safe handling of toxic and
corrosive chemicals and knowledge of the application of fire
prevention measures prescribed for laboratories.
Information on safety practices is provided with your instrument and opera-
tion manuals. Before using your instrument or accessories, you must thor-
oughly read these safety practices. This manual is written for laboratory
technicians / scientists skilled in the art.
For research purposes only. While clinical applications may be shown,
this instrument is not tested by the manufacturer to comply with the In
Vitro Diagnostics Directive.

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Unskilled, improper, or careless use of this instrument can create fire
hazards, or other hazards which can cause death, serious injury to
personnel, or severe damage to equipment and property. Observe all
relevant safety practices at all times. Only use the device for
applications that fall within the scope of the specified intended use.
Else the protective and safety equipment of the device could fail.
WEEE directive
All equipment of Antec Scientific which are subjected to the WEEE di-
rective shipped after August 13, 2005 are compliant with the WEEE mark-
ing requirements. Such products are labelled with the “crossed out
wheelie”, depicted on the left site.
The symbol on the product indicates that the product must not be
disposed as unsorted municipality waste.
Collection & recycling information
Please ship the instrument back to the manufacturer (Antec Scien-
tific, the Netherlands) at the end-of-life time of the product. The man-
ufacturer will take care of the proper disposal and recycling of the in-
strument at its facilities.
Shipping address for the end-of-life products:
Antec Scientific, Industrieweg 12, 2382NV Zoeterwoude, The Netherlands
In case of questions, or if further information is required about the collec-
tion & recycling procedure, please contact your local distributor.
ROHS directive
The ROXY Exceed is ROHS compliant and in conformity with Directive EU
2015/863 Restricted use of Hazardous Substances in electrical and elec-
tronic Equipment (ROHS).
Antec Leyden B.V. is an ISO 9001:2015 certified company.