Antec Scientific ROXY Exceed User manual

T [phone] | [email protected] | www.AntecScientific.com
Antec Scientific
Industrieweg 12
2382 NV Zoeterwoude
The Netherlands
ROXY Exceed
User Manual
211.0010, Edition 4, 2020

2
Copyright ©2020, Antec, The Netherlands. Contents of this publication may not be reproduced in any form or by any means
(including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent
from the copyright of the owner. Antec reserves the right to make changes to the design and specifications of the instrument
and this manual without prior notice.
DECADE Elite, DECADE Lite, ROXY, ALEXYS, ROXY Exceed, INTRO, FlexCell, ReactorCell, µ-PrepCell, ISAAC, HyREF,
SenCell and SynthesisCell are trademarks of Antec Scientific. Whatman™ (word and device) and Whatman™ (word only) are
trademarks of Whatman international Ltd. SOLVENT IFD™ and AQUEOUS IFD™ are trademarks of Arbor Technologies, Inc.
Clarity®, DataApex® are trademarks of DataApex Ltd. Microsoft®and Windows™ are trademarks of Microsoft Corporation. Ex-
cel is a registered trademark of the Microsoft Corporation. ChromeleonTM is a trademark of ThermoFisher Scientific. All other
trademarks are the property of their respective owners.
The software and the information provided herein is believed to be reliable. Antec shall not be liable for errors contained
herein or for incidental or consequential damages in connection with the furnishing, performance, or use of software or this
manual. All use of the software shall be entirely at the user’s own risk.

Declaration of Conformity
We Antec Leyden B.V., Zoeterwoude, The Netherlands, declare that the product:
ROXY Exceed ™ Potentiostat type 211
to which this declaration relates, is in conformity with the following CE directives:
2014/35/EU Low Voltage Directive (LVD)
2014/30/EU Electromagnetic Compatibility Directive (EMC)
2015/863/EU Directive on Restriction of the use of certain Hazardous Substances (RoHS)
2012/19/EU Directive on Waste Electrical and Electronic Equipment (WEEE)
Product standards used for demonstration of compliance:
EN 61010-1:2010
Safety requirements for electrical equipment for measurement, control, and laboratory use; part 1:
General Requirements.
EN 61010-2-010:2014
Safety requirements for electrical equipment for measurement, control, and laboratory use; part 2-
010: Particular requirements for laboratory equipment for the heating of materials.
EN 61326-1:2013
Electrical equipment for measurement, control and laboratory use - EMC requirements; part 1: Gen-
eral Requirements (Class B equipment).
EN 55032:2015
Electromagnetic compatibility of multimedia equipment
Emission requirements (Class B equipment).
Only use manufacturer-supplied cable(s) to connect with other devices. Thoroughly connect
shielding to common. Manufacturer will not accept any liability for damage, direct or indirect,
caused by connecting this instrument to devices and with cables which do not meet relevant
safety standards. All cables used should not exceed a length of 3 meters.
This DoC applies to above-listed product placed on the market after:
Date: January 31th, 2020
Dr. N.J. Reinhoud (managing Director)

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Table of contents
Symbols 7
Intended use 9
WEEE directive 10
ROHS directive 10
Safety instructions 11
Introduction 15
Instrument description 16
Installation 19
Storage requirements 19
Site Preparation Requirements 19
Unpacking 24
Mains connection 26
PC connection 26
Software 30
LC connections 31
Maintenance & Shutdown 35
Maintenance 35
Shutting down the system 37
ROXY Exceed control 39
Introduction 39
Overview of ROXY Exceed screens 40
Parameters 45
Dialogue Elite 50
Chromeleon CDS 52
Operation modes & Parameters 53
Three- or two electrode configuration 53
Internal organization 55
Dual flow cell control 55
Parameters 57
Filter 60
Measurement modes 64
DC mode 64
Pulse mode 65
Pulse mode -2 67
Scan mode 68
Optimization of working potential 69
Hydrodynamic and scanning voltammogram 71
Specifications ROXY Exceed 85
Rear panel I/O 93
USB B connector 94
LAN connector 94
VALVE connector 94
ANALOG DATA connector 100

CHAPTER 1 Table of contents 5
Digital I/O connector 103
Chassis grounding stud 110
Troubleshooting 113
Instrument errors 113
Dummy cell test 115
Potentiostat accessories 121
Index 124

6 211_0010 - ROXY Exceed user manual - rev 04

CHAPTER 1 Table of contents 7
Symbols
The following symbols might be used in this guide or may be found on the
instrument:
This sign warns about the risk of electric shock. It calls
attention to a procedure or practice which, if not adhered
to, could result in loss of life by electrocution. Do not pro-
ceed beyond a danger sign until the indicated conditions
are fully understood and met.
The warning sign denotes a hazard. It calls attention to a
procedure or practice which, if not adhered to, could re-
sult in severe injury, loss of life or damage or destruction
of parts or all of the equipment. Do not proceed beyond a
warning sign until the indicated conditions are fully under-
stood and met.
The caution sign denotes a hazard. It calls attention to a
procedure or practice which, if not adhered to, could re-
sult in damage or destruction of parts or all of the equip-
ment and/or erratic results. Do not proceed beyond a
cautions sign until the indicated conditions are fully un-
derstood and met.
The biohazard sign draws attention to the fact that use
of biological materials, viral samples may carry a signifi-
cant health risk.
The toxic hazard sign draws attention to the fact that use
of toxic solvents or samples may carry a significant
health risk.

8 211_0010 - ROXY Exceed user manual - rev 04
The hot surface sign calls attention to parts in the
instrument that must not be touched, as they may cause
burns.
This symbol indicates electrostatic discharge (ECD haz-
ard), damages to system, device, or components can oc-
cur if not properly grounded.
This symbol indicates that the waste of electrical and
electronic equipment must not be disposed as unsorted
municipal waste and must be collected separately.
Please contact the manufacturer or authorized repre-
sentative of the manufacturer for information concerning
the decommissioning of equipment.
A device or system marked with CE fulfills the product
specific requirements described in the European direc-
tives. This is confirmed in a Declaration of Conformity.
Certification marking for Canada and the USA issued by
MET labs, a Nationally Recognized Testing Laboratory
(NRTL). The certified device complies to the relevant UL
and CSA safety (61010-1) standards.
Frame or chassis ground terminal, which can be used as
to make additional external grounding connection.
The note sign signals additional information. It provides
advice or a suggestion that may support you in using the
equipment.
The attention sign signals relevant information. Read this
information.

CHAPTER 1 Table of contents 9
Intended use
The ROXY potentiostat in combination with flow-through reactor (µ-
PrepCell or ReactorCell) or batch reactor cells (SynthesisCell) is used for
controlled REDOX reactions up-front Mass Spectrometric detection. It can
be used in a wide range of applications, for example:
•Fast synthesis of metabolites (µ-preparative)
•Rapid risk assessments of drug-protein binding
•Signal enhancement in MS
•Electrochemical cleavage of proteins/peptides
•Reduction of disulfide bonds in proteins/peptides
•Oxidative stress/damage of proteins, DNA, lipids, etc.
This instrument is not specifically designed and intended for quantification
of analytes in LC, for that purpose the DECADE Elite/Lite electrochemical
detector is available. Operation of the ROXY potentiostat in combination
with a flow cell can involve the use of hazardous materials including corro-
sive fluids and flammable liquids. The instrumentation should only be oper-
ated by users with the following expertise:
•Completed degree as chemical laboratory technician or
comparable vocational training
•Fundamental knowledge of liquid chromatography equipment and
mass spectrometry
•Participation in an installation of the system performed by the
manufacturer or a company authorized by the manufacturer and
suitable training on the system and chromatography software.
•Knowledge and experience in the safe handling of toxic and
corrosive chemicals and knowledge of the application of fire
prevention measures prescribed for laboratories.
Information on safety practices is provided with your instrument and opera-
tion manuals. Before using your instrument or accessories, you must thor-
oughly read these safety practices. This manual is written for laboratory
technicians / scientists skilled in the art.
For research purposes only. While clinical applications may be shown,
this instrument is not tested by the manufacturer to comply with the In
Vitro Diagnostics Directive.

10 211_0010 - ROXY Exceed user manual - rev 04
Unskilled, improper, or careless use of this instrument can create fire
hazards, or other hazards which can cause death, serious injury to
personnel, or severe damage to equipment and property. Observe all
relevant safety practices at all times. Only use the device for
applications that fall within the scope of the specified intended use.
Else the protective and safety equipment of the device could fail.
WEEE directive
All equipment of Antec Scientific which are subjected to the WEEE di-
rective shipped after August 13, 2005 are compliant with the WEEE mark-
ing requirements. Such products are labelled with the “crossed out
wheelie”, depicted on the left site.
The symbol on the product indicates that the product must not be
disposed as unsorted municipality waste.
Collection & recycling information
Please ship the instrument back to the manufacturer (Antec Scien-
tific, the Netherlands) at the end-of-life time of the product. The man-
ufacturer will take care of the proper disposal and recycling of the in-
strument at its facilities.
Shipping address for the end-of-life products:
Antec Scientific, Industrieweg 12, 2382NV Zoeterwoude, The Netherlands
In case of questions, or if further information is required about the collec-
tion & recycling procedure, please contact your local distributor.
ROHS directive
The ROXY Exceed is ROHS compliant and in conformity with Directive EU
2015/863 Restricted use of Hazardous Substances in electrical and elec-
tronic Equipment (ROHS).
Antec Leyden B.V. is an ISO 9001:2015 certified company.

CHAPTER 1 Table of contents 11
Safety instructions
Adhere to the following standard quality control procedures and the follow-
ing equipment guidelines when using the ROXY Exceed potentiostat. The
following safety practices are intended to ensure safe operation of the in-
strument.
Working environment & safety
The intended use of the ROXY potentiostat is to perform controlled
REDOX reactions of target compounds (in a suitable liquid electrolyte me-
dium) up-front Mass Spectrometric detection in a GLP-approved environ-
ment under the specified environmental conditions. Operators using the
system should have the appropriate education an extensive understanding
of GLP rules and be skilled in the art. Use this system ONLY for the in-
tended use. Use of the system for any other purpose will cause unsafe sit-
uations.
System Operation
To assure optimal performance keep of the potentiostat we recommend
that the instrument is checked regularly and maintenance procedures are
carried out. Preventive maintenance contracts are available for that
Purpose. Please contact your local dealer or the nearest sales office for
more information.
Electrical safety
The removal of protective panels on the instrument can result in exposure
to potentially dangerous voltages. Therefore, disconnect the instrument
from all power sources before disassembly.
Untrained personnel should not open the instrument, this may only be
done by authorized service engineers. Replace or repair faulty insula-
tion on power cords immediately after discovery of the fault. Check that the
actual power voltage is the same as the voltage for which the instruments
are wired. Make sure power cords are connected to correct voltage

12 211_0010 - ROXY Exceed user manual - rev 04
sources: grounded AC power source, line voltage 100 –240 VAC. The in-
strument should be connected to a protective earth via a ground socket.
The ROXY Exceed must only be used with appliances and power sources
with proper protective grounding to prevent damage through build-up of
static electricity. The power source should exhibit minimal power transients
and fluctuations. If necessary connect to a filtered mains socket.
Replace blown fuses with fuses of proper type and rating as indicated on
the rear panel and as listed in the list of accessories and spares (appendix
D). The fuse holder is integrated in the mains connector. Ensure that the
instrument is never put in operation with fuses of a different type. This
could cause fire.
Thoroughly connect shielding to common. Manufacturer will not accept any
liability for damage, direct or indirect, caused by connecting this instrument
to devices and with cables which do not meet relevant safety standards.
Place the potentiostat on a flat and smooth surface. Do not block the venti-
lation holes located at the bottom and lower rear panel of the potentiostat.
Blocking these holes may impair the cooling capability of the power supply.
Solvents
The solvents used may be flammable, toxic or corrosive. The room in
which the system is installed should be well ventilated to prevent that sol-
vent vapors cause poisoning or ignite and cause a fire. Use of open fire in
the vicinity of this system must be strictly prohibited. Do not install the sys-
tem in the same room with any other equipment that emits or could poten-
tially emit sparks. Provide protective equipment near the instrument, when
USE ONLY WITH 250 V FUSES
EMPLOYER UNIQUEMENT
AVEC DES FUSIBLES DE 250V
Only use manufacturer-supplied I/O cable(s) to connect with other
devices.

CHAPTER 1 Table of contents 13
solvent gets into the eyes or on the skin, it must be flushed away immedi-
ately. Provide equipment, such eye wash stations and safety showers, as
close to system as possible. Use proper eye and skin protection when
working with solvents. Additional safety requirements or protection may be
necessary depending on the chemicals used in combination with this
equipment. Make sure that you understand the hazards associated with
the chemicals used and take appropriate measures with regards to safety
and protection. Sample containers (vials) should be sealed to minimize
any risks related to solvent vapor.
Biological Hazard
When you analyze biological fluids you need possible precautions and
treat all specimens as potentially infectious. Always wear protective
And gloves when handling toxic or biologically infectious samples to pre-
vent bio hazards or hazards while working with the ROXY Exceed. If ap-
plicable the instrument must be decontaminated before decommis-
sioning or shipment of the instrument for repair to Antec or its repre-
sentatives. When shipped to Antec every instrument has to be accompa-
nied with a decontamination form which should be completely filled in and
signed by the customer. Without this decontamination form the instrument
will not be processed by Antec (either repaired or disposed).
Waste disposal
Perform periodic leak checks on LC tubing and connections. Do not close
or block the drain in the oven compartment. Do not allow flammable and/or
toxic solvents to accumulate. Follow a regulated, approved waste
Disposal program. Never dispose of flammable and/ toxic solvents through
the municipal sewage system.
Using the ROXY Exceed in other ways than indicated in the manual or
defined by good laboratory practice may result in erratic or unsafe
operation.

14 211_0010 - ROXY Exceed user manual - rev 04

CHAPTER 1 Introduction 15
C H A P T E R 1
Introduction
Congratulations on your purchase of the ROXY Potentiostat. With more
than 25 years of experience in Electrochemistry (EC), Antec introduces a
new, dedicated Potentiostat for on-line EC/MS and EC/LC/MS. The ROXY
Potentiostat generates metabolites of drugs or xenobiotics, similar to those
generated during in vivo metabolic processes, in a significantly shorter
time span (seconds vs. days or weeks) without any interfering components
(no isolation steps required). The ROXY Potentiostat is based on state-of-
the-art electronics with a large voltage range of ± 4.9 V, and a push button
electrode regeneration program. Operational parameters and external
equipment can be controlled through programmable timed events. Conse-
quently, the generation of specific oxidation products, e.g., metabolites,
cleavage products, etc., and supreme control of any conceivable Redox
reaction is assured.
This manual covers the installation, set-up and operation of the ROXY
Exceed only. Detailed operation instructions for other peripheral LC
equipment and parts such as flow cells, Mass spectrometers, pumps,
auto samplers, valves, column heaters etc. are given in the manuals
accompanying those accessories.

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Instrument description
ROXY Exceed –Front side
#
Description
#
Description
1
Instrument housing
7
'+' and '-' value keys
2
LC tubing inlet/outlet
8
Cursor keys
3
Instrument door panel
9
Door handle (for opening door)
4
4 x 40 Ch LCD display
5
Function keys
6
<Enter> key

CHAPTER 1 Introduction 17
ROXY Exceed –Back side
#
Description
#
Description
1
Instrument rear panel
7
USB connector (USB B)
2
Type label (pn, sn etc.)
8
Fuse & power rating
3
Digital I/O connector (25-pins sub-D fem)
9
Mains switch/inlet
4
Analog data (9-pins sub-D fem)
10
Grounding stud
5
Valve connector (9-pins sub-D male)
11
Fuse compartment
6
LAN connector (RJ45 jack)
12
Ventilation holes

18 211_0010 - ROXY Exceed user manual - rev 04
ROXY Exceed –Oven compartment
#
Description
#
Description
1
Cell cabinet
8
Door lock
2*
Cell connector (9-pins sub-D fem)
9
Mounting hole for cell clamp (M4)
3
Top fan heater (intake)
10
Bottom fan heater (exhaust)
4
Door sensor
11
Mounting hole for column clamp (M3)
5
Mounting plate (for cells & columns)
12
Fuse compartment
6
Column clamp
13
Door panel, rear
7
Flow cell clamp (for SenCell)#
14
Type label
#) Note the shown cell (SenCell) is just an example, for ROXY other type of flow-through
reactor cells are used.
*) The standard ROXY Exceed potentiostat has 1 reactor cell connector, the DCC has two
cell connectors (DCC is the maximum cell configuration for the Exceed).

CHAPTER 2 Installation 19
C H A P T E R 2
Installation
Storage requirements
The ROXY potentiostat is shipped in one box to your facility with the fol-
lowing dimensions:
Make sure to have sufficient space to store the packed instrument under
the following storage conditions:
Site Preparation Requirements
It is evident that (for as far it is not specified in this document) the
installation site must comply with all applicable local laws and
regulations with regard to electrical and mechanical installations,
building safety, and use of potentially hazardous materials/chemical
and disposal thereof, etc.
For a successful onsite installation of the instrument, please arrange the
following requirements at your location in advance:
Personal Computer
In case the instrument is used via remote control by PC software (Dialogue
Elite or Thermo Chromeleon CDS) or a firmware (FW) update needs to be
performed a desktop computer is required with the following requirements:
•Free LAN port (onboard, PCI, PCI express or PCI-X)
•Free USB port (required for FW updates)
•Microsoft Windows 7 Operating System or higher*
*) An US or European Microsoft® Windows™ version is recommended. The
Windows operating system should be updated with the latest Service Packs.
Equipment
Dimensions storage box
ROXY potentiostat
59 (D) x 41 (W) x 56 (H) cm (23.2 x 16.1 x 22.0 in)
Parameter
Requirement
Storage temperature
-10 –50 oC (14 –122 °F)
Storage humidity
20 –80%, non-condensing

20 211_0010 - ROXY Exceed user manual - rev 04
Further information about software packages and requirements please visit
the Antec website www.antecscientific.com.
*) In your antivirus SW, turn off the option "Check Files
at Change" for the relevant Dialogue Elite data storage directory.
The PC for control and data-acquisition of the system should be placed in
the vicinity of the ROXY potentiostat, within a distance of max 2.5 meter.
Laboratory requirements
Your instrument is intended for indoor use only in an industrial or commer-
cial environment (EN55011 group 1 class B ISM equipment). It is suitable
for the following categories: Installation category II, Pollution degree 2,
equipment class I.
Table I. Environmental specifications
Parameter
Requirement
Operating temperature
10 –35 oC (50 –104oF)
Maximum Altitude
2000 meter (7500 ft)
Operating humidity
20 –80%, non-condensing
Installing software requires a computer with administrator access.
Make sure that the PC and its USB ports is authorized/able to install
third-party software. Inform your IT department well in advance to
arrange authorization to avoid unnecessary delays during the
installation.
For uninterrupted operation of a ROXY EC system and control software
Antec advises to turn off:
- Screensavers
- (USB, LAN) hibernate mode
- Auto hard disk shut down (energy saving)
- Automatic Windows updates
- Avoid exhaustive scanning by virus scanners*