Baker SterilGARD e3 SG404 User manual

P.O.DrawerE/161GatehouseRoad/Sanford,Maine04073/USA
800‐992‐2537/207‐324‐8773/Fax:207‐324‐3869/[email protected]/bakerco.com
389D000 Rev B i
OPERATOR’S MANUAL
Class II, Type A2
Biological Safety Cabinet
MODELS:
SG404 / SG504 / SG604
SG404-INT / SG504-INT / SG604-INT
SG404-J / SG504-J / SG604-J
Revision Date Description
B 11/12/14 Changed Warranty Statement
This manual includes information for proper operation of this cabinet.
We recommend that it be kept near the cabinet for ready reference.

P.O.DrawerE/161GatehouseRoad/Sanford,Maine04073/USA
800‐992‐2537/207‐324‐8773/Fax:207‐324‐3869/[email protected]/bakerco.com
389D000 Rev B ii
INTRODUCTION AND WELCOME
It is a pleasure to welcome you to the growing number of customers who own and operate Baker laboratory
products. As the inventors of the laminar flow biological safety cabinet and the leaders in the field, Baker
people take special pride in providing a product that is designed for maximum performance.
Your new SterilGARD®e3cabinet includes many unique features which are included to give you superior
performance, more simple maintenance and lower life cycle cost. The SterilGARD® e3is a Class II, Type A2
biosafety cabinet that provides personnel, product, and environmental protection. The SterilGARD® e3is
suitable for research and clinical diagnostic work involving tissue culturing of possibly infectious samples, IV
drug preparations and other pharmaceuticals that could have adverse health effects on operators and other
techniques requiring a contamination-free atmosphere.
In addition to the high quality you expect from all Baker equipment, this model has been designed to provide
the lab user with the most comfortable work environment possible. The ergonomic design will help prevent
repetitive motion injury, reduce fatigue and lab accidents and enhance productivity.
Baker’s exclusive e3 (Energy Efficient Engineered) product line also offers reductions in noise and electrical
power consumption without sacrificing the superior performance of a Baker cabinet.
The adequacy of a cabinet for user safety should be determined on-site by an industrial hygienist, safety
officer or other qualified person. Remember that you, the owner and user, are ultimately responsible and that
you use your cabinet at your own risk.
We recommend that this manual, along with the factory test report, be kept near the cabinet for convenient
reference by operators and qualified maintenance personnel. If you have any questions about the use or care of
your new SterilGARD® e3cabinet, please do not hesitate to contact our Customer Service Department
at 1-800-992-2537 for assistance or e-mail us at [e-mail].
Sincerely,
David Eagleson
President
The Baker Company, Inc.

P.O.DrawerE/161GatehouseRoad/Sanford,Maine04073/USA
800‐992‐2537/207‐324‐8773/Fax:207‐324‐3869/[email protected]/bakerco.com
389D000 Rev B iii
TABLE OF CONTENTS
I – FUNCTION OF THE SterilGARDe3...................................................................................................1
II - CABINET DESIGN...................................................................................................................................2
Standard Features....................................................................................................................................3
Cabinet Primary Function.................................................................................................................................................3
Applicable Standards........................................................................................................................................................3
Cabinet Pressure Plenums.................................................................................................................................................3
Motor/Blower Capacity, Cabinet Supply Blower.............................................................................................................3
Manual Air Balance..........................................................................................................................................................3
Cabinet Filters ..................................................................................................................................................................3
Easy Filter Access ............................................................................................................................................................3
Work Area Design............................................................................................................................................................3
Front Access High Velocity Air Slots ..............................................................................................................................3
Towel Guard.....................................................................................................................................................................4
Work Surface....................................................................................................................................................................4
Drain Pan..........................................................................................................................................................................4
View Screen......................................................................................................................................................................4
View screen Access Opening ...........................................................................................................................................4
Work Area Lighting..........................................................................................................................................................4
Electronic Ballast..............................................................................................................................................................4
Ground Fault Circuit Interrupter (115V only)..................................................................................................................4
Laboratory services...........................................................................................................................................................4
Alarms ..............................................................................................................................................................................4
Timers...............................................................................................................................................................................4
Padded Bypass Armrest....................................................................................................................................................5
UniPressure™ Plenum......................................................................................................................................................5
Motor/Blower Assembly ..................................................................................................................................................5
Cable Port.........................................................................................................................................................................5
ReadySAFE™ Mode........................................................................................................................................................5
Power/Processor Fault Alarm...........................................................................................................................................5
Optional Features.....................................................................................................................................6
Adjustable Cabinet Stand .................................................................................................................................................6
IV Bar...............................................................................................................................................................................6
UV light............................................................................................................................................................................6
Floor and Wall Seismic Anchoring ..................................................................................................................................6
FlexAIR™ Canopy Exhaust Connection (CEC) ..............................................................................................................6
Digital Display Package Option (DDP)............................................................................................................................6
Auxiliary Wiring Options.................................................................................................................................................6
III - PROPER CABINET USE.......................................................................................................................7
Operation and Controls...........................................................................................................................7
Blower..............................................................................................................................................................................7
Fluorescent Light..............................................................................................................................................................8
Auxiliary Outlets ..............................................................................................................................................................8
Ultraviolet (UV) Light [Optional] ....................................................................................................................................8
Alarm Reset......................................................................................................................................................................8
Alarm Conditions.....................................................................................................................................9
Sash Alarm .......................................................................................................................................................................9
Power/Processor Fault Alarm...........................................................................................................................................9
Double Proximity Sensor Fault Alarm .............................................................................................................................9
Low-flow Exhaust Alarm.................................................................................................................................................9
Alarm Summary Table .....................................................................................................................................................9
Cabinet Timer Functions.......................................................................................................................10

P.O.DrawerE/161GatehouseRoad/Sanford,Maine04073/USA
800‐992‐2537/207‐324‐8773/Fax:207‐324‐3869/[email protected]/bakerco.com
389D000 Rev B iv
15 minute increment programming: ...............................................................................................................................10
1 hour increment programming:.....................................................................................................................................10
Cable Ports..............................................................................................................................................10
Cable Ports (continued).........................................................................................................................11
Start-up Procedure.................................................................................................................................11
Working in the Cabinet.........................................................................................................................12
Using Ancillary Equipment...................................................................................................................13
Reacting to Spills....................................................................................................................................13
Cleaning and Disinfecting Stainless Steel ............................................................................................13
Simple Cleaning .............................................................................................................................................................13
Disinfection ....................................................................................................................................................................13
Cleaning the Drain Pan...................................................................................................................................................14
Decontamination ....................................................................................................................................14
About the HEPA Filters.........................................................................................................................15
Warranty.................................................................................................................................................16

389D000 Rev B 1
I – FUNCTION OF THE SterilGARDe3
The SterilGARD®e3 is a Class II, Type A2, Biological Safety Cabinet (BSC) as classified by
NSF/ANSI Standard 49. This Class of BSC may operate with or without an external facility exhaust system.
The cabinet relies on the flow of air to provide personnel, product and environmental protection. Personnel
protection (containment) is provided by a minimum intake air velocity at the front access opening. Product
protection is provided by the HEPA filtered supply air in the cabinet work area. Environmental protection is
provided by the exhaust HEPA filter.
Room air enters the front access opening of the cabinet at a minimum of 100 FPM [0.508m/sec] then enters the
front work surface perforation. Most of the HEPA filtered supply air passes through a diffuser but some of the
air is directed down the back side of the view screen creating a high velocity air curtain at the front access
opening. The HEPA filtered supply air in the work area splits at the work surface. Some of the air enters the
rear work area perforation while the remainder of the air enters the front work surface perforation. The air is
pulled through the drain pan area, up the rear and side wall plenums, to the cabinet blower. The blower pushes
the air into the positive pressure plenum. At that point most of the air is pushed through the supply HEPA filter
while the remainder is exhausted out the exhaust HEPA filter and through a perforated filter protector at the top
of the cabinet. [Reference Figure1]
Figure 1: Airflow design
EXHAUST
INTAKE
(ROOM AIR)
WORK SURFACE
SUPPLY HEPA
FILTER
POSITIVE PRESSURE
PLENUM
= ROOM AIR
= CONTAMINATED AIR
= HEPA FILTERED AIR
EXHAUST HEPA
FILTER
DRAIN PAN AREA
MOMENTUM AIR
CURTAIN

389D000 Rev B 2
II - CABINET DESIGN
Figure 2: Cabinet Feature Locations
CABINET BALANCING
MANUAL DAMPER CONTROL
EXHAUST HEPA FILTER
PROTECTOR
FASCIA PANEL
EXHAUST HEPA FILTER
POSITIVE PRESSURE
PLENUM
SUPPLY HEPA
FILTER
REMOVABLE WORK
SURFACE AND SUPPORTS
FLUORESCENT LAMP
/ LIGHT CANOPY
SLIDING VIEWSCREEN
10 DEGREE SLOPE
SUPPLY DIFFUSER /
FILTER PROTECTOR
AUXILIARY
OUTLETS
BLOWER OUTLET
PETCOCK LOCATION
PADDED BYPASS
ARMREST
MEMBRANE SWITCH
CONTROL PAD
OPTIONAL UV LIGHT
DRAIN VALVE
BACKWALL & SIDWALLS
RETURN AIR PLENUM
WITH TOWEL GUARD

389D000 Rev B 3
Standard Features
Cabinet Primary Function
The SterilGARD®e3is designed as a Class II, Type A2, Biological Safety Cabinet (BSC) as classified by
NSF/ANSI Standard 49. This Class of BSC may exhaust to the room or through a canopy exhaust connection
located at the top of the cabinet into an external facility exhaust system.
Applicable Standards
Except as noted elsewhere in this document or in the factory test report, this cabinet meets the requirements of
and is tested to UL61010-1, 2nd Edition, CAN/CSA-C22.2 No. 61010-1, 2nd Edition, NSF/ANSI Standard 49-
2012 and IEC/EN61326-1(2006), IEC/EN61000-3-2(2000), IEC/EN61000-3-3(2001) providing NSF listing and
CE Mark (APPROVAL PENDING)
Cabinet Pressure Plenums
The cabinet work area is surrounded by negative pressure plenums, all external seals are under negative
pressure, and all internal positive pressure plenum seals are surrounded by negative pressure plenums. All
pressure plenums are ruggedly constructed in order to provide strength, durability, air tight seals and resistance
to deterioration.
Motor/Blower Capacity, Cabinet Supply Blower
The SterilGARD®e3cabinet is designed to automatically compensate for an increase in pressure drop across the
filters without reducing total air flow rate by more than 10%. The air flow capacity of the cabinet is measured
by the ability to provide a nearly constant volume of air as the filter resistance to airflow increases.
Manual Air Balance
The SterilGARD®e3cabinet has a manual internal balancing damper that compensates for down flow and
exhaust/intake imbalances. WARNING
This damper must only be adjusted by a qualified technician who has had proper training and has the
proper equipment for checking the air balance inside the cabinet.
Cabinet Filters
Both supply and exhaust filter inside the cabinet are scan-tested HEPA filters. They are 99.99% effective on
removal of most penetrating particles (mpp) of 0.3 micron size. Each filter is leak checked after installation in
the cabinet and prior to shipment.
Optional factory installed Supply and exhaust ULPA Filters are available. The proven efficiency of ULPA
filters is 99.999% for removal of most penetrating particles (mpp) 0.1 to 0.2 microns in size.
Easy Filter Access
All filters are front accessible for ease of service. The supply and exhaust filters can be removed from the front
of the cabinet through an access panel located behind the fascia panel in the front of the cabinet.
WARNING
Only qualified technicians should replace filters.
Work Area Design
The interior side and rear work area walls are constructed from a single piece of stainless steel. Inside corners
are constructed with a smooth radius to help prevent buildup of contaminants and facilitate cleaning.
Front Access High Velocity Air Slots
High velocity air slots are placed around the perimeter of the cabinet access opening. Slots are located at the
front of each sidewall at the access opening, and horizontally along the top of the access opening behind the
view screen. The purpose of the slots is to improve the cabinet integrity by capturing any particulates at these
critical transitions.

389D000 Rev B 4
Towel Guard
The towel guards are located under the work surface at the bottom rear and sides of the return-air plenums.
Acting as a protective screen, integral to the interior walls, they help prevent wipes and other paper materials
from being drawn into the blower system. They need to be kept clean at all times.
Work Surface
The work surface is constructed of corrosion resistant stainless steel. This surface is finished to reduce light
reflection. It is also recessed to retain spills. The work surface and work surface supports are removable
allowing access to the drain pan.
Drain Pan
The drain pan is constructed of corrosion resistant, stainless steel. The drain pan is designed with a smooth
radius along all four bottom corners to facilitate cleaning and disinfection. Drainage is provided by a stainless
steel ball valve located on the underside of the drain pan.
View Screen
The cabinet view screen is constructed of UV resistant safety plate glass. It is designed to move vertically to
allow access to the work area and it is counterbalanced for ease of movement. The proper work access opening
is clearly marked with arrows on each side of the view screen and the position of the view screen is monitored
by an optical sensor activating the sash alarm when not properly positioned.
View screen Access Opening
The SterilGARD® e3 standard view screen access opening is 8”[203 mm].
Other optional factory configurations available with NSF listing are 10”[254 mm] and 12”[305 mm] access
openings.
Work Area Lighting
The work area is illuminated with one fluorescent lamp external to the work area that provides a typical light
intensity of 100 foot-candles [1076 lux] at the work surface.
Electronic Ballast
The SterilGARD® e3features solid-state electronic ballasts for the fluorescent light and optional UV light.
These ballasts increase reliability, efficiency and service life with lower heat output.
Ground Fault Circuit Interrupter (115V only)
The outlets on the cabinet are protected by a ground fault circuit interrupter (GFCI).
Laboratory services
The cabinet is equipped with one petcock and one plugged penetration located in the right sidewall.
Additional factory installed services are available on either side wall.
The standard external plumbing connections are located at the bottom of the right side of the cabinet.
Other optional configurations available from the factory are located on the rear or top of the cabinet as well as
on the left side of the cabinet.
Alarms
The cabinet has an audible and visual alarm to alert the operator of unsafe conditions. The annunciated alarms
are Sash Alarm, Power Fault Alarm and Double Sash Sensor Fault Alarm.
Timers
The cabinet has programmable timers to control the operation of the Fluorescent lights, Accessory Outlets, and
UV Light Option. The timers can be programmed in 15 minute or 1 hour increments.

389D000 Rev B 5
Padded Bypass Armrest
The Armrest pad is made out of EPDM sponge material which is resistant to most chemicals as well as UV
exposure. The pad is held in place with an adhesive and can be easily removed for cleaning. It can also be
autoclaved. When the view screen is fully closed, the perforated armrest provides an air bypass that allows air
to continue to enter the front Work Surface perforations. This helps minimize noise.
UniPressure™ Plenum
The telescoping positive pressure plenum provides a more even clamping force on the HEPA filter frames and
provides more uniform down flow air.
Motor/Blower Assembly
The motor and blower are built as a single assembly in order to minimize vibration.
Air Pressure Gauge
The cabinet has an analog air pressure gauge which displays the cabinet’s negative static pressure.
The displayed value during operation should remain consistent with the recorded value in the most recent
certification report. However if drastic change is noted, this should be cause for investigating the reason.
This device is not intended to be used for air flow set-point verification.
Cable Port
One cable port is located in the cabinet’s right side wall. Cable ports provide a safe means of introducing power
and/or data cables, siphoning tubes, etc. into the work area of the cabinet without having to use the front view
screen access opening. A sealing plug is provided for each port, for use when the port is not required or when
the cabinet is being decontaminated.
Additional cable ports are available in either left or right side walls.
ReadySAFE™ Mode
The “ReadySAFE™ Mode” is a feature that reduces the total airflow and energy consumption when the cabinet
is not being used. Operation of the cabinet in “ReadySAFE™ Mode” maintains personnel, product and
environmental protection.
Power/Processor Fault Alarm
The Power/Processor Fault Alarm provides a visual and audible alarm when the system experiences a power
outage or the microprocessor/controller has a fault.

389D000 Rev B 6
Optional Features
Adjustable Cabinet Stand
The channel stand has adjustable legs and leg levelers. The legs provide three fixed height adjustments of
24 1/8” [613 mm], 28 5/8” [727 mm] and 34 5/8” [880 mm] with leg levelers in minimum position. The leg
leveler provides an additional 2” [51 mm] of height adjustment.
IV Bar
The cabinet can be equipped with an intravenous (IV) Bar to facilitate hanging required materials.
UV light
The SterilGARDe3can be equipped with an ultraviolet (UV) light that provides a minimum intensity of 40
microwatts per cm2at the work surface with a new bulb.
Floor and Wall Seismic Anchoring
Floor and Wall Seismic anchoring plates are available with and without California OSHPD pre-approval.
FlexAIR™ Canopy Exhaust Connection (CEC)
The FlexAIR™ canopy exhaust connection with integrated alarm provides the cabinet with a safe exhaust
connection to building exhaust systems.
Digital Display Package Option (DDP)
The Digital Display Package provides a durable touch screen display for cabinet controls, cabinet monitoring
and cabinet alarms. One digital pressure monitor is included for airflow monitoring.
Auxiliary Wiring Options
Additional wiring options are available to provide external connection of the status of certain cabinet conditions
to an external HVAC system. The following wiring options are offered:
Blower Switch Status Wiring Package which provides an external analog signal regarding the status of
the cabinet blower (either ON or OFF)
Cabinet Monitor Wiring Package which provides the blower operational status as well as sash alarm
status or ReadySAFE™ status.