Baker SterilGARD SG404 User manual

P. O. Drawer E / 161 Gatehouse Road / Sanford, Maine 04073 / USA
800-992-2537 / 207-324-8773 / Fax: [phone] / [e-mail] /
bakerco.com
389D001 Rev G
SERVICE MANUAL
Class II, Type A2
Biological Safety Cabinet
MODELS:
SG404 / SG504 / SG604
SG404-INT / SG504-INT / SG604-INT
SG404-JPN / SG504-JPN / SG604-JPN
SG404-AUS / SG604-AUS
M-models are UL listed but not NSF listed
This manual includes information for proper service of this cabinet.
We recommend that it be kept near the cabinet for ready reference.

389D001 Rev G 2 of 94
TABLEOFCONTENTS
I - SPECIFICATIONS .....................................................................................................................................3
Exterior Dimensions .................................................................................................................................3
Unit Weights .............................................................................................................................................3
Plumbing Service ......................................................................................................................................3
Electrical Service ......................................................................................................................................4
Exhaust Flow Specifications ....................................................................................................................5
Exhaust Duct Specifications ....................................................................................................................5
Environmental Conditions ......................................................................................................................6
Symbols and Terminology .......................................................................................................................6
II - INSTALLATION ......................................................................................................................................7
Preparing the SterilGARDe3 for use ....................................................................................................7
Checking the cabinet upon arrival ......................................................................................................7
Preparing the cabinet for installation ..................................................................................................7
Location within the laboratory ...........................................................................................................7
Venting into the room .........................................................................................................................7
Venting outside the facility ................................................................................................................7
Final Connections and Tests....................................................................................................................8
Channel Stand Installation Procedure ...................................................................................................9
1.Channel Stand Assembly Procedure: .......................................................................................10
2.Channel Stand Leg Assembly Procedure: ................................................................................13
III - CERTIFICATION .................................................................................................................................15
Field Certification Procedures ..............................................................................................................15
Cabinet Airflow Verification .................................................................................................................15
HEPA Filter Leak Test Procedure .......................................................................................................20
Airflow Smoke Pattern Test ..................................................................................................................25
Site Installation Assessment Tests ........................................................................................................26
IV - MAINTENANCE ...................................................................................................................................27
HEPA Filter Replacement Procedure ..................................................................................................27
Changing the Cabinet Exhaust HEPA Filter .......................................................................................32
Changing the Cabinet Supply HEPA Filter .........................................................................................33
View screen Removal Procedure ..........................................................................................................34
Fluorescent Lamp Replacement ...........................................................................................................37
Ultraviolet Lamp Replacement .............................................................................................................38
Touchpad Replacement .........................................................................................................................39
Cabinet Pressure Monitor .....................................................................................................................40
Replacement Parts List ..........................................................................................................................43
Ladder Schematics .................................................................................................................................45
Electrical Panel Assemblies ...................................................................................................................61
Unit Wiring .............................................................................................................................................71
Auxiliary Blower Switch Status Wiring Option ..................................................................................74
Auxiliary Cabinet Monitoring Wiring Option ....................................................................................75
FlexAIR Canopy Exhaust Connection Wiring ....................................................................................76

389D001 Rev G 3 of 94
I - SPECIFICATIONS
Exterior Dimensions
SG404 SG504 SG604
Foot Print (w x f-b) 53 3/4 x 30 11/16”
[1,365 x 779 mm]
65 3/4 x 30 11/16”
[1,670 x 779 mm]
77 3/4 x 30 11/16”
[1,975 x 779 mm]
Height (Without Stand) 61 3/4”
[1,568 mm]
Min Height (with Channel Stand) 86 1/8”
[2,188 mm]
Max Height (with Channel Stand) 98 3/8”
[2,500 mm]
Ship Height (with Channel Stand)
(le
g
s & filter protector removed)
77 3/16”
[1,960 mm]
Unit Weights
Base unit weight SG404 SG504 SG604
Bench Top 582 Lbs.
[264 k
g
]
714 Lbs.
[324 k
g
]
771 Lbs.
[350 k
g
]
With Channel Stand 655 Lbs.
[297 k
g
]
790 Lbs.
[358 k
g
]
850 Lbs.
[386 k
g
]
With Channel Stand and Casters 665 Lbs.
[302 k
g
]
800 Lbs.
[363 k
g
]
860 Lbs.
[390 k
g
]
With Hydraulic Lift Stand 792 Lbs.
[359 k
g
]
927 Lbs.
[420 k
g
]
993 Lbs.
[450 k
g
]
Base Shipping weight
Bench Top 712 Lbs.
[323 k
g
]
864 Lbs.
[392 k
g
]
951 Lbs.
[432 k
g
]
With Channel Stand 785 Lbs.
[356 k
g
]
940 Lbs.
[426 k
g
]
1,020 Lbs.
[463 k
g
]
With Channel Stand and Casters 795 Lbs.
[361k
g
]
950 Lbs.
[431 k
g
]
1,030 Lbs.
[467 k
g
]
With Hydraulic Lift Stand 922 Lbs.
[418 k
g
]
1,077 Lbs.
[489 k
g
]
1,163 Lbs.
[528 k
g
]
Plumbing Service
Standard laboratory air, vacuum, gas and water service connections are rated for 75 psig [52 KPa] maximum
pressure and connections are provided by 3/8” OD Brass Tube fitting connectors. Optional stainless steel and
black iron service connections are rated for 75 psig [52 KPa] maximum pressure

389D001 Rev G 4 of 94
Electrical Service
A single dedicated circuit is required to power this cabinet. This circuit shall provide the protective earth ground
for the cabinet.
All electrical wiring to the cabinet should comply with the national electrical code and any applicable local
electrical codes at the site of installation.
The power cord is the main disconnect device for the unit. The unit should be positioned in a manner that
allows easy access to the power cord connection to the electrical utility.
The cabinet’s operating current (less outlets and options) is listed in the accompanying test report.
The cabinet may be provided with an optional lift that requires 2 Amps intermittent duty.
The lift duty cycle is 1 minute on, 9 minutes off.
The cabinet may be provided with an optional UV lamp.
The cabinet may be provided with an optional 24V DC power supply for low voltage options
Service Requirements
100V 100 V AC, 20 A, 50/60 Hz, 1Ø
16 A maximum useable current
115V 115 V AC, 20 A, 60 Hz, 1Ø
16 A maximum useable current
220V 220 V AC, 16 A, 50/60 Hz, 1Ø
13 A maximum useable current
Circuit Protection
100/115 V Internall
y
protected with a 250 V, 20 A circuit breake
r
220 V Internall
y
protected with a 250 V, 16 A circuit breake
r
Power Cord
100 V/115V One 14’ [4.27 M] power cord with 20A plu
g
, NEMA 5-20P
220 V One 4 M power cord with listed plug for the destination
countr
y
Outlets .
100 V
Two 115 V AC, duplex outlets. The outlets on this circuit
are protected by a self-resetting circuit breaker allowing for
a total of 5A on all outlets.
115 V
Two GFCI protected, 115 V AC, duplex outlets. The outlets
on this circuit are protected by a self-resetting circuit
b
reaker allowin
g
for a total of 5A on all outlets.
220 V
Two 220 V AC, outlets. The outlets on this circuit are
protected by self-resetting circuit breakers allowing for a
total of 5A on all outlets

389D001 Rev G 5 of 94
Exhaust Flow Specifications
Vent to Room SG404 SG504 SG604
8” [203 mm] Access Opening Height 285 CFM
[135 L/Sec]
355 CFM
[168 L/Sec]
455 CFM
[215 L/Sec]
10” [254 mm] Access Opening Height 355 CFM
[168 L/Sec]
445 CFM
[215 L/Sec]
545 CFM
[257 L/Sec]
12” [305 mm] Access Opening Height 404 CFM
[191 L/Sec]
550 CFM
[260 L/Sec]
611 CFM
[288 L/Sec]
FlexAIR Canopy Exhaust
Connection SG404 SG504 SG604
Concurrent Balance Value
Minimum/Maximum
8” [203 mm] Access Opening Height 290/663 CFM
[137/313 L/Sec]
360/750 CFM
[170/354 L/Sec]
460/845 CFM
[217/399 L/Sec]
10” [254 mm] Access Opening Height 360/701 CFM
[170/331 L/Sec]
450/820 CFM
[212/387 L/Sec]
550/945 CFM
[260/446 L/Sec]
12” [305 mm] Access Opening Height 415/871 CFM
[196/411 L/Sec]
560/850 CFM
[264/401 L/Sec]
644/1,114 CFM
[304/526 L/Sec]
Exhaust Duct Specifications
FlexAIR Canopy Exhaust
Connection SG404 SG504 SG604
Exhaust Duct Static Pressure
Minimum/Maximum
8” [203 mm] Access Openin
g
Hei
g
ht
8” Exhaust Duct Diameter -0.10/-0.32” W.G.
[-25
/
- 80 Pa]
-0.15/-0.62” W.G.
[-37
/
-154 Pa]
-0.15/-0.74” W.G.
[-38
/
-185 Pa]
10” Exhaust Duct Diameter -0.04/-0.17” W.G.
[-10
/
-43 Pa]
-0.05/-0.16” W.G.
[-13
/
-40 Pa]
-0.07/-0.18” W.G.
[-18
/
-45 Pa]
12” Exhaust Duct Diameter -0.03/-0.08” W.G.
[-8
/
-20 Pa]
-0.04/-0.12” W.G.
[-10
/
-30 Pa]
-0.06/-0.13” W.G.
[-15
/
-33 Pa]
10” [254 mm] Access Openin
g
Hei
g
ht
8” Exhaust Duct Diameter -0.15/-0.62” W.G.
[-38
/
-155 Pa]
-0.18/-0.60” W.G.
[-45
/
-149 Pa]
-0.20/-0.78” W.G.
[-50
/
-195 Pa]
10” Exhaust Duct Diameter -0.05/-0.18” W.G.
[-13
/
-45 Pa]
-0.09/-0.35” W.G.
[-23
/
-87 Pa]
-0.11/-0.24” W.G.
[-28
/
-60 Pa]
12” Exhaust Duct Diameter -0.04/-0.10” W.G.
[-10
/
-25 Pa]
-0.05/-0.16” W.G.
[-13
/
-40 Pa]
-0.09/-0.16” W.G.
[-22
/
-40 Pa]
12” [305 mm] Access Openin
g
Hei
g
ht
8” Exhaust Duct Diameter -0.17/-0.75” W.G.
[-42
/
-187 Pa]
-0.22/-0.74” W.G.
[-55
/
-184 Pa]
-0.30/-0.79” W.G.
[-75
/
-197 Pa]
10” Exhaust Duct Diameter -0.07/-0.30” W.G.
[-15
/
-75 Pa]
-0.09/-0.24” W.G.
[-22
/
-60 Pa]
-0.14/-0.36” W.G.
[-35
/
-90 Pa]
12” Exhaust Duct Diameter -0.05/-0.25” W.G.
[-12
/
-62 Pa]
-0.07/-0.17” W.G.
[-17
/
-42 Pa]
-0.11/-0.30” W.G.
[-27
/
-75 Pa]

389D001 Rev G 6 of 94
Environmental Conditions
The cabinet is designed for use in the following conditions:
 Indoor use
 Altitudes up to 6562 Ft (2000 meters)
 Temperature range from 41 F (5C) to 104 F (40C)
 Maximum relative humidity 80% for temperatures up to 88° F (31°C) decreasing linearly to 50% at 104
F (40C)
 Main supply voltage: Rated voltage stated in cabinet ratings ±10%V AC
 Transient over voltage according to installation category (OVERVOLTAGE CATEGORIES) II per
UL/IEC61010-1, 2nd Edition
 Pollution degree 2
Symbols and Terminology
Protective Earth: Any terminal intended for connection to external protective conductor for
protection against electric shock in case of a fault.
General Caution: Refer to instruction manual for information regarding personnel and environment
protection.
!

389D001 Rev G 7 of 94
II - INSTALLATION
Preparing the SterilGARDe3 for use
Checking the cabinet upon arrival
Upon receipt of your new SterilGARD ® e3 cabinet, inspect the exterior of the crate, box and/or skid. If there is
any visible damage, note it on the receiving slip and immediately report it to the delivering carrier.
Remove the outer packing material and inspect the cabinet itself. If any concealed damage is found please report
this to the delivery carrier. A claim for restitution needs to be filed within 15 days.
Due to the risk of mishandling by trucking companies, Baker has removed certain parts of the cabinet and has
packed them separately. These items are listed on the packing slip, which accompanies the cabinet. Please check
the packing slip carefully to be sure that all items have been located.
Preparing the cabinet for installation
Installation of this cabinet should be carried out in accordance with appropriate OSHA regulations and those
other regulatory agencies having jurisdiction. Carefully follow the instructions outlined in the shipping change-
out document 390D001which is attached to the view screen.
WARNING
Do not move the view screen prior to removing the shipping hardware from the counterweight box
located inside the right side panel
Location within the laboratory
Proper placement within the laboratory is essential. The ideal location for any biological safety cabinet is in a
dead-end corner of the laboratory away from personnel traffic, vents, doors, windows, and any other sources of
disruptive air currents. Published research from The Baker Company and unpublished tests performed at the
National Cancer Institute show that if a draft or other disruptive air current were to exceed the intake velocity of
the cabinet, contamination can enter the work area or escape from it (for more information, contact Baker).
Venting into the room
If the cabinet exhausts air into the laboratory, it is important that there is adequate space between the top of the
cabinet and the ceiling. A solid ceiling located too close to the exhaust filter will restrict the air and limit the
intake velocity. The exhaust filter guard, an inverted “V” shaped perforated steel plate included with your
SterilGARD e3 cabinet, will help protect the exhaust filter from objects being dropped on it and provide the
necessary distance between exhaust opening and ceiling to ensure proper operation. If the filter guard is not
installed, the cabinet exhaust opening should be no closer than 3” [76.2mm] from the ceiling, to enable proper
airflow. In order to conduct proper maintenance a 6” [152 mm] clearance to the ceiling is recommended.
Venting outside the facility
Whenever possible, the cabinet exhaust should be canopy connected to its own separate exhaust system. If it
must be ducted into a ganged exhaust system, make sure that the system is not a recirculating type. The building
exhaust system should contain provisions to adjust the building system flow and pressure.

389D001 Rev G 8 of 94
The FlexAIR Canopy Exhaust Connection has a 12” [305 mm] diameter connection with a 10” [254mm]
diameter spun inner baffle. It mounts directly over the exhaust filter and has flaps to reduce the amount of room
air being exhausted. A sensor that detects when the flap opens during low duct exhaust conditions triggers a low
flow exhaust alarm in the cabinet.
Optional reducing collars are available to adapt the FlexAIR exhaust connection to smaller duct diameters.
Final Connections and Tests
1. The plumbing connection to the service petcocks must be made with great care because the effluent
from a safety cabinet may be biologically hazardous. Consult the facility safety officer regarding these
connections to assure they are properly connected and filtered if required. When present, petcocks are
factory piped within the cabinet. The external connection consists of a 3/8” compression tube fitting at
the rear, top, or bottom of the cabinet outer sidewall seal panel. Qualified personnel with proper
materials and technique should make connection to plant utilities per NFPA
2. Check that the drain valve is closed or the drain coupling is properly capped.
3. Check for proper installation of the cable port
4. Connect the cabinet to the dedicated power supply circuit as noted in Section I under “Electrical
Service”
5. If so equipped, connect the relay(s) of the optional cabinet monitor wiring package to the external
HVAC building automation system
6. Position the view screen at the correct access opening height indicated by the arrow labels on each side
of the view screen guides.
7. Turn on the blower. The indicator light will illuminate and the running blower will make an audible
sound.
8. Turn on the fluorescent light. The indicator light will illuminate along with the interior work area. Check
the following conditions:
 The fluorescent light will not operate unless the blower switch is on.
 The fluorescent light will not operate when the view screen is closed.
9. If your cabinet has the optional ultraviolet light (UV), lower the view screen to its fully closed position
and turn the UV light on to make sure it is operational.
NOTE: The UV light option features an interlock that prohibits its operation unless the view
screen is fully closed. The fluorescent lamp and UV lamp will not operate simultaneously.

389D001 Rev G 9 of 94
Channel Stand Installation Procedure
Document: 389D002 Rev A
Release Date: 03/24/14
This procedure describes the proper assembly of the SGX04 basic channel stand and the correct installation
underneath the cabinet. It provides step by step instructions for the channel stand installation in two instances:
1. The Channel Stand Assembly Procedure when the stand ships from the factory separately from the
cabinet
2. The Channel Stand Leg Assembly Procedure when the channels stand is factory attached to the
bottom of the cabinet
Tools required: 7/16” deep socket, 9/16” wrench or socket, level
Parts List:
Item Number Part Number Description Qty
1 389W401/390W401/365W401 Channel stand, brace 1
2 390W410 Channel stand, side 2
3 389P401 Channel stand, gusset 2
4 40273 Bolt, carriage,¼”-20 x 2” long 4
5 10392 Bolt, hex head, 3/8”-16 x 1” long 24
6 11283 Flat washer, ¼” 4
7 11286 Flat washer, 3/8” 24
8 11311 Lock washer, 3/8” 24
9 10512 Hex lock nut, ¼” 4
10 390P406 with 43312
(390P416 with 43345/43346)
Adjustable leg with leg leveler (or
optional castor) 4
11 390P414 Channel , cover 4
12 11776 Plug, leg 8
NOTE: Caster stand option requires four modified leg assemblies (Not shown).

389D001 Rev G 10 of 94
1. Channel Stand Assembly Procedure:
Step 1:
Remove stand components from rear of
cabinet skid if shipped as shown.
Remove items 4-11 from the separate
box shipped with the cabinet.
If cabinet is factory shipped on channel
stand please see channels stand leg
assembly procedure
Step 2:
Gather all stand components, hardware, and tools.
Step 3:
Place stand sides on a flat floor surface.
Place the stand back between the sides
so that the open part of the channel is
facing down towards the floor. Place
the braces
as shown.
Step 4:
Using the 3/8”x 1” hex head bolt with
lock and flat washers [Items 5, 7, & 8],
attach the sides and back by inserting
the bolts thru the (4) holes located in
the channel stand back into the already
installed rivnuts in the two sides. (2)
bolts per side. Do not tighten any of
these bolts until you have all (4)
threaded in slightly. Tighten bolts after
this is done.
Channel stand
brace
Channel stand
side
Channel stand,
brace
3/8” Hardware
Flip the stand 90º to
access lower hole
Channel stand
gusset

389D001 Rev G 11 of 94
Step 5:
With stand assembly complete, rotate
stand upside down. Attach the legs by
inserting them into the channels from
the front.
The telescoping legs can now be
adjusted to the desired height
requirement. This is done by aligning
the stand holes with the holes in each
leg, then sliding the leg cover over the
channel. Secure the leg assembly with
3/8” hardware on both sides.
Do not tighten bolts yet, so the cover
can slide up and down in the slots.
Cover the exposed threaded holes in the
legs with plug.
NOTE: If the cabinet is not in its final
room location you may want to perform
the leg adjustment after assembling the
cabinet to the stand and moving it to the
room (see step 11).
Step 6:
Rotate the stand assembly to the upright
position so that the leg levelers are now
resting on the floor.
Adjustable leg
with leveler
Channel, cover
3/8” Hardware
Both sides
[Items 5, 7, & 8]
Exposed unused
hole;
Plug with item 12

389D001 Rev G 12 of 94
Step 7:
Lift the cabinet off the skid and high
enough to allow clearance for the stand
assembly to be positioned under it.
Once the stand is positioned under the
cabinet, install (4) ¼”x 2" carriage bolts
into the key way slots located under the
cabinet in each of the (4) corners. The
cabinet is now ready to be lowered onto
the stand. There are (4) holes on the top
of the stand which the bolts must be
guided through. Be careful not to let the
bolts drag on the edge of the holes
while lowering as burrs may occur on
the bolts and cause threading problems.
Step 8:
With the cabinet resting on top of the
stand assembly, place a ¼” flat washer,
and hex lock nut onto each of the bolts.
Push the stand as far forward as
possible, then tighten all ¼” hex lock
nuts. A 7/16” deep socket is required for
this.
Step 9:
With the cabinet secured on top of the
stand and at the correct height, slide the
leg covers up so the top edge will touch
the drain pan. Tighten all 3/8” hex bolts
on both sides.
Step 10: if not already installed, attached the drain valve to the bottom of the drain pan by removing the
handle and threading into the nipple using thread seal tape. Position the valve such that the handle will be
facing the front of the cabinet (see picture in step 7. Attach the handle and secure with screw.
Step 11:
The lift device should be removed if not being used to move cabinet into position within the room. If the lift
device is mobile it may be used to maneuver cabinet into the desired location. A blanket could also be placed
under the leg levelers while sliding the cabinet to avoid scuffing the flooring.
¼”-20 carriage
bolt Channel
Cabinet
¼” Hardware
Drain

389D001 Rev G 13 of 94
2. Channel Stand Leg Assembly Procedure:
Step 1:
If cabinet is factory shipped on channel
stand remove components
4 -11 that are separately shipped with
cabinet.
Step 2:
Lift cabinet off the skid and high enough to
allow clearance for the adjustable legs to
be assembled to the front and rear of the
channel stand sides.
For a 261/8” to 281/8” [664 mm-714 mm]
work surface height align the upper two
holes in the leg with the upper two holes in
the stand.
For a 305/8” to 325/8” [778mm-829 mm]
work surface height align the upper two
holes in the leg with the middle two holes
in the stand.
For a 365/8” to 385/8” [931 mm-981 mm]
work surface height align the upper two
holes in the leg with the lower two holes in
the stand.
Attach channel cover, align slots with
holes and use 3/8” hardware on both sides
to secure legs.
Skip to Step 7
Step 3:
If lifting of the complete cabinet is not possible, carefully slide the cabinet off the skid onto a moving blanket
or similar protective material in order to protect floor finish.
Slide the cabinet into its final destination prior to assembly of the adjustable legs.
Channel, cover
Adjustable leg
with levele
r
3/8” Hardware
Both sides
[Items 5, 7, & 8]

389D001 Rev G 14 of 94
Step 4:
Carefully lift up one end of the cabinet by
at least 6” [150 mm] and block into
position using a safe and secure method.
Insert adjustable leg into channel on front
and rear side of cabinet and secure it as
described in Step 2.
Repeat this step at the other side of the
cabinet
Warning: if caster option is provided
make sure to chock the wheels before
lifting the other side.
Step 5:
If the work surface height needs to be
raised to 305/8”[778 mm] height, lift up one
end of the cabinet again by another 6” [150
mm] and unscrew the 3/8” leg bolts on both
sides of each leg and slide down the leg
until the lower holes in the leg are aligned
with the lower two holes in the channel.
Secure legs with the hardware.
Repeat this step at the other side of the
cabinet.
Step 6:
If the work surface height needs to be raised to 385/8”[981 mm] height, repeat Step 5.
Step 7: Use a level and level the cabinet
by turning the leg leveler feet on each
adjustable leg.
Warning: do not extend the leveler feet
beyond the marked groove.
Insert plugs to all exposed threaded holes
in the legs.
Groove at maximum
extension

389D001 Rev G 15 of 94
III - CERTIFICATION
Field Certification Procedures
The following Field Certification Procedures shall be conducted by a trained biosafety cabinet technician.
Cabinet Airflow Verification
The following calibrated test instruments and tools are required to perform this field certification.
 Direct Inflow Measurement Device (For Primary Inflow Method) (optional)
 Thermal Anemometer (For Secondary Inflow Method and Downflow air velocity measurement)
 Aerosol Photometer and Laskin Nozzle Generator
 Smoke Visualization Device (Smoke tubes)
 Amperage meter
 1/4” to 7/16” Standard (English) ratchet or socket style tools. A ll bolts are Imperial, not Metric
 Adjustable Wrench
 Good quality adhesive tape
 Step ladder
 Tape measure with Imperial measurements
 Pocket calculator
 Flat head and cross head screwdrivers
The SterilGARD Biosafety Cabinet is shipped with a protective laminate sheet covering the exhaust HEPA
filter. Removal of this sheet shall be done by the certifier, NOT THE INSTALLER, because of potential
damage during installation. If this protective sheet is not present after installation, inspection of the exhaust
HEPA filters is strongly recommended. Filter replacement maybe necessary if damage found cannot be
repaired.
Step 1: If equipped with FlexAIR Canopy Exhaust Connection, be sure to have the exhaust air turned on and at
the specified exhaust flow range before beginning the cabinet airflow validation procedure. (See Baker Document
BAKD066 for exhaust airflow requirements)
To meet manufacturer’s specifications, all methods and acceptance
criteria must be followed. No alternative or secondary methods for
measuring cabinet airflow other than what is stated in this service manual
and the cabinet data plate shall be used to validate the performance of this
biosafety cabinet.

389D001 Rev G 16 of 94
Step 2: With the cabinet sash at the correct operating height indicated by the red/white arrows, measure the
downflow velocities with a thermal anemometer and fixed ring stand. Refer to the data plate label on front of
cabinet for downflow locations and velocities. Below is a sample of the data plate label.
Before making any required cabinet damper and/or fan speed adjustments, measuring the inflow velocity is
strongly advised.
Step 3: Two methods are available for verifying the cabinet intake velocity of the SterilGARD biosafety cabinet;
Option 1: Primary Method using Direct Inflow Measurement Device (DIM)
Option 2: Secondary Method using Thermal Anemometer (Constricted Method)
NOTE: No other method to verify the cabinet intake velocity is allowed.
Option 1: Primary Method using Direct Inflow Measurement Device (DIM)
Directions:
Leaving the armrest in place, attach and seal the perimeter of a Direct Instrument Measurement (DIM) device to
the front access opening of the biosafety cabinet. Be sure to seal off the slots below the padded armrest. Take a
minimum of 5 consecutive inflow readings in cubic feet per minute (CFM) or in liters per second (L/S) for the
inflow average. The average intake velocity (V) is then calculated by dividing average inflow (Q) by the area (A)
of the front access opening. V=Q/A
HOTWIRE RANGE
NOTE: REMOVE ARMREST
Orientation
5.7" (145mm)Typ
Traverse Locations
1.5" (38mm)
Lower sash to 3" (76mm)
SG404: CLASS II TYPE A2
Seal direct reading instrument to front access opening.
PRIMARY METHOD
SECONDARY METHOD (3" Sash)
Calculated Intake Velocity FPM (m/sec)
RANGE 100 to 110 FPM (0.51 TO 0.56 m/sec)
(10" Sash)
X
2
2
INTAKE VELOCITY
Probe
2
2
+
3" (76mm)Typ
above bottom edge of sash
(8") 5.38"(137mm)
NOTE: REMOVE IV BAR AND UV LIGHT
Measure velocities in horizontal plane 4" (102mm)
3" (76mm)
ZONE 1
ZONE 2
BACK OF SASH
Plan View
REAR WALL
Zone 2= 42 to 52 FPM (.21 to .26 m/sec)
Zone 1= 47 to 57 FPM (.24 to.29 m/sec)
DOWN FLOW VELOCITY 43122
0.958 FT (0.09m )
3.194 FT (0.30m )
ZONE AVERAGES
3" Traverse
Vel. Avg. 5 FPM
(.025 m/sec)
318 FPM to 350 FPM
(1.62 to 1.78 m/sec)
5.66" Typ
(144mm)
7" (178mm) Typ
6" (152mm) Typ
8" (203mm) sash = 256 to 282 CFM (121 to 133 L/sec)
10" (254mm) sash = 319 to 351 CFM (151 to 166 L/sec)
12" (305mm) sash = 383 to 422 CFM (181 to 199 L/sec)
(8" Sash)
X
2
2
2
2
+
0.958 FT (0.09m )
2.560 FT (0.24m )
3" Traverse
Vel. Avg. 5 FPM
(.025 m/sec)
(12" Sash)
X
2
2
2
2
+
0.958 FT (0.09m )
3.833 FT (0.36m )
3" Traverse
Vel. Avg. 5 FPM
(.025 m/sec)
254 FPM to 280 FPM
(1.29 to 1.42 m/sec)
380 FPM to 420 FPM
(1.93 to 2.13 m/sec)
7" (178mm) Typ
(10") 5.19"(132mm)
(12") 5.00"(127mm)
(Sash)

389D001 Rev G 17 of 94
Option 2: Secondary Method using Thermal Anemometer (Constricted Method)
Remove the armrest and lower the cabinet sash to an access opening height of 3 inches (76 mm). Using a thermal
anemometer with or without Bakers’ secondary method fixture (#333A714), orient the probe tip facing upward at
the center of the vertical access opening (1.5” or 38 mm). Starting 3 inches from the left or right of the opening,
measure the intake velocity across the sash at the locations specified on the cabinet data plate. Refer to data label
affixed to the cabinet fascia panel for more information.
(NOTE: If the INTAKE velocity average is out of range, use the chart below to determine what balancing
adjustments are required to meet the airflow specification. If an adjustment is necessary, the downflow and
inflow values must be measured and recorded again.
APPROACHES TO BALANCING THE STERILGARD CABINET
IF THEN
INTAKE
Velocit
y
DOWNFLOW
Velocit
y
Low Low Turn up the speed control
High High Turn down the speed control
Low High Open cabinet exhaust damper
High Low Close cabinet exhaust damper
Low / High Ok Adjust both speed control and cabinet exhaust damper
Ok Low / High Adjust both speed control and cabinet exhaust damper
Ok Ok No adjustment Needed
3 Inch Method Template
F
ree hand Procedure

389D001 Rev G 18 of 94
SPEED CONTROLLER ADJUSTMENTS:
If a speed controller adjustment is necessary;
Step 1: Remove thumbscrews or shipping bolts from front fascia panel and raise, engaging the left and right
panel supports.
Step 1: Using a ¼” nut driver or ratchet wrench, remove the aluminum cover from the
electrical mounting board located on the left hand side. Set the left DIP switch down to
take out of Automatic mode. A green LED will ‘flash’ indicating this manual setting.

389D001 Rev G 19 of 94
Step 2: Slowly trim the pot to achieve the necessary airflow;
Clockwise to increase speed and counter clockwise to decrease
speed. After fan speed adjustment, wait a total of one minute before
switching the motor controller back to ‘automatic mode’. The green
LED on motor controller will be ‘solid green’ indicating this
automatic setting.
Step 3:
DAMPER ADJUSTMENTS:
If an internal damper adjustment is necessary, loosen the damper locking
nut on top of the cabinet then open or close using a straight blade
screwdriver. Tighten locking nut when desired adjustment is complete. If
no other adjustments are necessary to obtain airflow balance, retake inflow
and downflow readings verifying range acceptance. (See Service Manual
for more information)

389D001 Rev G 20 of 94
HEPA Filter Leak Test Procedure
NOTE 1:
The Primary Method for the scanning the HEPA Filters in the SterilGARD is using an aerosol
photometer and Laskin nozzle generator. As an alternative, a discrete particle counter may be used in
accordance with appropriate standards i.e. IEST-RP-CC034.3, ISO 14664-3.
CAUTION! THIS TEST REQUIRES THE USE OF AN AEROSOL GENERATING DEVICE
WHICH MAY SET OFF LABORATORY SMOKE DETECTORS IF USED CARELESSLY.
SPECIAL PRECAUTIONS SHOULD BE TAKEN TO AVOID AN ACCIDENTAL FIRE ALARM
CONDITION.
Step 1: Verify that the biosafety cabinet is at the proper airflow setpoint using the procedure outlined in
this Service Manual.
Step 2: If equipped, remove the germicidal light and IV Bar. Next remove the supply diffuser above the
cabinet work area by first removing two 11/32” lock nuts. Lower the front of the supply diffuser and
carefully slide it off of the rear locating pins and remove from the cabinet. Caution: Avoid hitting the
delicate supply filter media.
CAUTION!
POTENTIALLY CONTAMINATED AREA!
BEFORE PROCEEDING, A RISK ASSESSEMENT SHALL BE PERFORMED TO
IDENTIFY POTENTIAL HAZARDS WITHIN THE BIOSAFETY CABINET. IF
REQUIRED A SURFACE DECONTAMINATION OR SPACE
DECONTAMINATION MAY BE NECESSARY BEFORE PERFORMING THIS
PROCEDURE. PROPER PPE SHALL BE USED AS INSTRUCTED BY LAB
S
AFETY
O
FFI
C
E
R
.