Baker Bugbox Ax User manual

Bugbox Ax User Manual UM-053 R1.0 1
Bugbox Ax
Anaerobic Workstation
UM-053
Affix Serial Number Sticker Here

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Product Summary
The Baker anaerobic workstations are designed specifically to help microbiologists cope with
rising workloads and provide the best primary isolation rates.
Features and Benefits
•Compact size - Your personal workstation
•Quick and easy direct access with the gloveless, cuffed Ezee Sleeve system.
•Energy saving lighting. Read plates easily under perfect illumination without O2 exposure.
•Petri dish holders for quick plate transfer included.
•Optimum cell environment
oAccurate temperature control - from ambient + 5˚C to 45˚C.
oAccurate and automated humidity control - no dry spots.
oPalladium catalyst maintains anaerobic environment - plus anaerobic colour-
indicator strips verify anoxic conditions.
oEzee Sleeve system allows access without disrupting atmosphere within the
chamber.
•Economic and reliable long-term savings
•Standard dual gas operation - low gas consumption and running costs.
•Lower cost per plate compared to anaerobic jars.
•Minimal maintenance and downtime - with annual or biennial preventative maintenance
kits available.
Note: The use of the word Interlock in this manual is medical terminology, and refers to the
environmental working area between both chambers. It does not refer to the engineering safety
interlock switch mechanism.

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Table of Contents
Product Summary...........................................................................................................................2
Features and Benefits....................................................................................................................2
Safety instructions.............................................................................................................6
1. Regulatory compliance ......................................................................................7
North American Region.......................................................................................8
Precautions..............................................................................................................8
Symbols.....................................................................................................................9
Weight and dimensions....................................................................................10
Transport and storage ...................................................................................................11
Location and handling of the Bugbox Ax workstation.......................................11
Environmental Operating Conditions......................................................................11
Service Requirements ....................................................................................................12
Electrical Supply Requirements ....................................................................12
Gas Supply Requirements ...............................................................................13
Workstation overview ....................................................................................................14
Workstation layout.............................................................................................14
Component layout..............................................................................................14
Front view...................................................................................................14
Rear view ....................................................................................................15
Left side view –Standard humidifier...............................................16
Right side view..........................................................................................17
Control system layout.......................................................................................18
Control panel ............................................................................................18
Temperature control..........................................................................................19
Interlock Purge .....................................................................................................20
Humidity control..................................................................................................21
Aquasorb Sachets...................................................................................21
Settings Menu.......................................................................................................22
Commissioning Menu ...........................................................................23
Time & Date Settings..............................................................................24

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Offsets..........................................................................................................25
Data Log ......................................................................................................26
Anaerobic Monitoring (Optional Extra)......................................................27
O2 Calibration.......................................................................................................29
Micro SD Card............................................................................................30
Environmental gas composition control ................................................................31
Environmental control overview...................................................................31
Using the anoxic indicator strips ..................................................................31
Using the workstation.....................................................................................................32
Using the interlock..............................................................................................32
Interlock overview ..................................................................................32
Opening the interlock outer door......................................................32
Opening the interlock inner door ......................................................32
Transferring material into the workstation chamber via the
interlock..................................................................................................................33
Removing material from the workstation chamber via the
interlock..................................................................................................................33
Hand access to the main chamber..............................................................34
Vacuum Operation .............................................................................................35
Workstation Entry ...............................................................................................37
Workstation Exit...................................................................................................39
Rear shelf................................................................................................................40
Optional extras.....................................................................................................40
Single Plate Entry System (SPES) ......................................................41
Internal power socket............................................................................42
Cable gland ...............................................................................................42
Universal cable gland............................................................................42
Gas sample port.......................................................................................43
Vacuum port .............................................................................................43
Cleaning and maintenance .........................................................................................45
Cleaning the workstation.................................................................................45

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Cleaning agents ......................................................................................45
Cleaning procedure –during and after each use ......................45
Cleaning procedure –deep clean ....................................................46
Maintaining the workstation –End user maintenance........................47
Replacing the detox and catalyst sachets....................................47
Replacing the mains plug fuse –UK users only ..........................48
Replacing the mains fuses ..................................................................49
Service requirements ........................................................................................50
Spare parts and accessories ..........................................................................50
Overview.....................................................................................................50
Spare parts and cleaning agents .....................................................51
Accessories ...............................................................................................51
Workstation malfunction.................................................................................52
Common problems and solutions................................................................52
Workstation general problems .........................................................52
Gas consumption/ environmental control problems...............53
Warranty information ....................................................................................................54
Contact details..................................................................................................................55
Please read this manual carefully before using the Bugbox Ax and familiarise yourself with all
aspects of using the workstation. The Baker Company (Baker) or Ruskinn Technology Ltd
(Ruskinn) does not accept responsibility for accidents to personnel or damage to the Bugbox Ax
workstation resulting from incorrect use.
The Bugbox Ax has been specifically designed and developed to meet the requirements of all
laboratories. The unit is ergonomically laid out to ensure maximum operator comfort and
optimization of space for media preparation, manipulation, and analysis.
A heater system maintains the temperature within the workstation between ambient +5°C to
45°C, which is set by the user.
Many unique features of the Bugbox Ax workstation are covered in detail in this manual. It is
recommended that the user be fully conversant with the instruction and procedures, and that the
operator familiarises themselves with all aspects and functions of the system before it is
commissions to maintain optimum performance.

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Safety instructions
The Baker Company do not take any responsibility for damages caused by using the equipment
for other purposes than described in this user manual.
•The mains appliance coupler and plug are the AC mains supply isolation device and must be
easily accessible when installed.
•In case of emergency disconnect the Bugbox Ax from the AC Mains Outlet.
•Ensure that the connecting cable is not squeezed or bent when the unit is being installed or
moved.
•All installation work and adjustments to the unit must be carried out by qualified personnel.
Work performed by persons with insufficient technical knowledge may adversely affect the
performance of the unit or cause physical injury or damage to the equipment.
•All servicing and repairs must be carried out by a qualified customer service engineer. Only
genuine spare parts must be used.
•In case of damage to the Bugbox Ax disconnect the workstation from the mains outlet and
contact your local distributor.
•All covers and lids must only to be removed by a qualified service engineer.
•Nothing should be placed on the top of the workstation.
•A power cord supplied with the workstation and should be used to connect to the mains outlet.
If a replacement is required it must be adequately rated for the application.
•All cables and pipes should be routed to ensure that they do not pose a trip hazard.
•Mains supply Voltage fluctuation must not exceed ±10% of the nominal Voltage.
•Gas regulators must be used for each gas supply. A 2 stage regulator is recommended with a
maximum supply pressure of 5 Bar. Over pressure could damage the workstation.
•Only the gases specified in this user manual may be used.
•All gas bottles must be adequately secured before connection to the workstation.
•The maximum power rating of the internal sockets must not be exceeded.
•The end user is responsible for all materials and equipment places inside the workstation.
•Before connecting any equipment to the internal mains sockets ensure that the equipment has
or is connected to a protective earth. If in doubt please contact the equipment manufacturer.
•The workstation must not be operated at an ambient temperature over 30°C.
•The cooling fan covers and cooling vents must not be covered or blocked.
•The weight limit for the workstation internal floor is 30kg and must be evenly distributed.
•The weight limit for the interlock is 5kg and must be evenly distributed.
•There should be no naked flames close to the workstation.
•The use of radioactive materials if strictly prohibited.
CAUTION: Asphyxiation Risk
The Bugbox Ax uses Nitrogen (N2) and Hydrogen in Nitrogen (H2N2) as part of normal use with the
volume released externally is inconsequential. In the event of a leak or malfunction this gas
release may become excessive. DO NOT OPERATE this unit in a SMALL ENCLOSURE such as a small
room or walk-in closet. An accidental release of Nitrogen or Carbon Dioxide could create an
asphyxiating atmosphere in a small space.
If the equipment is not use in a manner specified by the manufacture, the protection provided by
the equipment may be impaired.
Failure to adhere to these safety instructions could cause serious injury and will invalidate the
workstation warranty. Ruskinn technology limited accepts no responsibility for any accident,
injury or loss caused by unsafe operation of the workstation.

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1. Regulatory compliance
CE Marking - for sales in the EU & NI
This product has been tested and complies with EU Directives 2014/35/EU (Low Voltage),
2014/30/EU (Electromagnetic Compatibility), and 2011/65/EU (RoHS as amended by EU 2015/863)
UKCA Marking –for Sales in GB
This product also complies with the following Statutory Instruments:
WEEE:
This equipment must be disposed of in accordance with the Waste from Electrical and Electronic
Equipment (WEEE) Directive
This product must not be treated as household waste. Instead, it shall be handed over to an
appropriate collection point for the recycling of electrical and electronic equipment.
If in doubt, please return this equipment to Ruskinn Technology Ltd who will correctly dispose of it
for you. We strongly recommend that this product is returned to RTL at the end of its useful life.
SI 2016 No. 1101
The Electrical Equipment (Safety) Regulations 2016
SI 2016 No. 1091
The Electromagnetic Compatibility Regulations 2016
SI 2012 No. 3032
The Restriction of the Use of Certain Hazardous Substances in
Electrical and Electronic Equipment Regulations 2012

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North American Region
This product is UL 61010-1 Listed, and CSA C22.2 No. 61010-1 under file number E113911.
This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used
in accordance with the instruction manual, may cause harmful interference to radio
communications. Operation of this equipment in a residential area is likely to cause harmful
interference in which case the user will be required to correct the interference at his own expense.
Precautions
The Bugbox Ax has been tested and approved to EN61010-1, 61326-1, and 61326-2-6. This means
that the Bugbox Ax meets or exceeds the requirements for General and IVD electrical Laboratory
equipment in terms of its levels of emitted electromagnetic (EM) radiation and its susceptibility
to electromagnetic radiation from other devices. It should be noted that the Bugbox Ax may be
affected by high levels of stray EM radiation from other electronic devices (even those which
comply with relevant CISPR emission standards) that are being used in close proximity to it.
WARNING: This system may cause radio interference or may disrupt the operation of nearby
equipment. It may be necessary to take mitigation measures, such as re-orienting or relocating
the equipment or shielding the location.

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Symbols
Before using the Bugbox Ax, please ensure that you are familiar with the symbols.
Symbol
Meaning
Refer to user manual.
~
Alternating current
O
Off
I
On
Functional Earth Connection
Protective Earth Connection
This product complies with the essential EEA requirements for Electrical
Safety and Electromagnetic compatibility as set out in the EMC directive
2004/108/EC and the Low Voltage Directive 2006/95/EC
Caution, do not remove covers. No end user serviceable parts behind covers.
Please refer to this manual in all cases where this symbol appears, in order to
find out the nature of the Potential Hazard and actions to be taken in order to
avoid the Hazard.
Warning, this equipment contains high voltage circuitry.
Contains material or substances that may be hazardous to human health.
Please refer to your local biohazardous material handling procedure for
further advice on the handling and disposal of these items.
InvivO2contains hazardous components and must not be disposed of at a
household waste site. Instead it should be taken to the appropriate collection
point for the recycling of electrical and electronic equipment.
USB socket
Date of manufacture in format
YYYY MM
Table 1: List of Symbols

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Weight and dimensions
The Bugbox Ax workstation weighs approximately 45kg. Figure 1 lists the dimensions of the Bugbox
Ax workstation;
Figure 1: Bugbox Ax workstation dimensions
External width
790 mm
External height
872 mm
External depth
679 mm
Workstation chamber internal width
540 mm
Workstation chamber internal height
535 mm
Workstation chamber internal depth
546 mm
Interlock internal width
149 mm
Interlock internal height
285 mm
Interlock internal depth
253 mm
Interlock outer door clear opening width
110 mm
Interlock inner door clear opening width
146 mm
Interlock inner door clear opening height
245 mm
Note: The interlock is designed for use with Baker Company Large Petri dish holders.

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Transport and storage
When not in use, the Bugbox Ax Workstation must only be stored within a temperature of between
0°C and 30°C
Storage outside of this range may damage the workstation.
Location and handling of the Bugbox Ax workstation
The Bugbox Ax should only be installed or relocated by a qualified engineer. To arrange
installation or relocation please contact your local distributor.
The mains appliance coupler and plug are the AC mains supply isolation device and must be
easily accessible.
For optimal performance, the Bugbox Ax should not be installed directly beneath Air Conditioning
outlets. This can cause excessive humidity to collect on the inside surface of the front screen.
Environmental Operating Conditions
The Bugbox Ax Workstation should only be operated under the following environmental
conditions:
•Temperature –Between 15⁰C and 30⁰C
•Humidity –Between ambient and 90% RH, Non-Condensing
The workstation must be located in a well-ventilated area.

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Service Requirements
Electrical Supply Requirements
The workstation must be connected to a mains power supply. A power cord is supplied to connect
the workstation to the mains supply. If an alternative power cord is used must be rated
appropriately for the power requirements of the workstation, refer to Table 2. The workstation
must be connected to a protective earth.
To ensure safe operation of the workstation, it must be connected to a supply of the correct
voltage and frequency as stated on the rating label shown at the rear of the unit. The mains
supply voltage fluctuations must not exceed +/- 10% of the nominal mains voltage. The input
voltage and frequency for Bugbox Ax workstations are:
Voltage Range
Frequency
Nominal Power
220V to 240V AC
50/60Hz
560W
100V to 120 V AC
50/60Hz
430W
Table 2: Electrical Service Requirements
Please note that the applicable ratings for the Workstation configuration are detailed the
labelling at the rear of the product as shown in Figure 2.
Figure 2: Bugbox Ax Rear Connections
1. Gas Supplies
2. Residual Current Circuit Breaker (RCCB)
3. Mains Input Connector (C19)
4. Footswitch Connector
5. Mains Input Fuses
6. Earth Stud
2
1
5
4
3
6

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The Workstation is double pole fused (Live and Neutral) for protection against over current draw.
The fuses fitted are based on the voltage range applicable, see Table 3 for details:
Voltage Range
Mains Input Fusing
220-240 Vac
T3.15A H250V
100 Vac, 110-120 Vac
T5A H250V
Table 3: Fuse Ratings
Gas Supply Requirements
The workstation gas supplies are located at the rear of the unit as shown in Figure 2.
For standard physiological environment operation of the workstation the input gas requirements
are:
Gas
Symbol
Specification
Regulator Output
Pressure
Nitrogen
N2
Oxygen Free
(Industrial or medical)
42to 72.5psi
(3 to 5 bar)
5% Hydrogen in
Nitrogen*
H2/N2
Maximum of 5.5%
Hydrogen in Nitrogen
(Industrial or medical)
42 to 72.5psi
(3 to 5 bar)
Table 4: Standard Gases
For the gas supply we recommend direct connections of the gas bottle regulator to ensure
minimal pressure drops and flow rate restrictions.
The gas regulator should provide a minimum supply pressure of 3 bar gauge. The maximum
supply pressure permissible is 5 bar gauge. A supply pressure greater than this will damage
internal components of the Bugbox Ax and will invalidate the warranty.
Only the above stated gases are to be used with the workstation. Failure to comply with this may
cause the product to become hazardous.
* - Recommended Hydrogen concentrations are compliant to BS EN ISO 10165:2010 (Classification
of Flammable Gasses). The use of Hydrogen concentrations up to a maximum of 10% in Nitrogen
(N2) is the responsibility of the user and must be assessed by the user in accordance to local
safety regulations at the installation location.

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Workstation overview
Workstation layout
The Bugbox Ax workstation consists of 2 main areas; the workstation chamber and the interlock
chamber. The workstation chamber is the main working area of the workstation. Access to the
workstation chamber is provided by the glove ports and the interlock. The interlock consists of the
interlock chamber, with an inner and an outer door. The Bugbox Ax workstation is controlled via
the control panel.
Component layout
Please familiarise yourself with the layout of your Bugbox Ax workstation.
Front view
Figure 3 shows the front view of the Bugbox Ax workstation;
Figure 3: Bugbox Ax workstation front view
1. Glove ports (shown without Ezee Sleeves).
2. Interlock outer door.
3. Control panel.
2
3
1

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Rear view
Figure 4 shows the rear view of the Bugbox Ax workstation;
Figure 4: Bugbox Ax workstation rear view

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Left side view –Standard humidifier
Figure 5 shows the left side view of the Bugbox Ax workstation with the standard humidifier;
Figure 5: Bugbox Ax workstation left side view
1. Condenser fan Intake –Do not obstruct
2. Condensate tank drain pipe.
3. Universal Cable Gland (optional extra)
4. Single Plate Entry System (optional extra)
5. Glove Port Stowage Bracket
Note: Place condensate trough (provided in the accessory kit) below the condensate tank drain
pipe to collect any excess water from the overflow tube.
3
4
1
2
5

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Right side view
Shows the right side view of the Bugbox Ax workstation;
Figure 6: Bugbox Ax workstation right side view
1. Interlock Fan Intake –Do not obstruct
1

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Control system layout
Please familiarise yourself with the control system layout of your Bugbox Ax workstation.
Control panel
Figure 7 shows the control panel of the Bugbox Ax workstation;
Figure 7: Bugbox Ax workstation control panel
1. Temperature Set Point
2. Temperature Reading
3. ANO2Low Alarm Icon
4. N2Low Alarm Icon
5. Trend Log Button
6. Time / Date / Settings Button
7. Humidity Set Point
8. Humidity Reading
9. Interlock Purge Button
10. Humidity Decrease Button
11. Humidity Increase Button
12. Gas Control status- Green =Active. Red =Deactivated
1
3
5
6
7
8
9
4
2
10
11
12

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Temperature control
The Bugbox Ax workstation can control the workstation chamber temperature between ambient
plus 5oC and 45oC.
The temperature in the main chamber can be set using the temperature controller settings on the
touchscreen.
Setting the temperature;
1. Press the temperature controller dialog on the touchscreen.
2. The Temperature Control Input Keypad will appear. Enter the desired temperature in
intervals of 0.1oC:
3. Press the ENTER button, the keypad will close and the temperature control system will
make the necessary adjustments

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Interlock Purge
The Bugbox Ax workstation has a 9.8 litre interlock that is used for transferring materials and
samples into and out of the workstation main chamber. The interlock can be purged of oxygen by
pressing the interlock purge button. This pre-conditions the interlock atmosphere to not affect the
internal workstation atmosphere.
To run an interlock purge first ensure that both the inner and outer door are closed.
Then press the interlock purge button which will begin a 30 second purge.
A countdown timer displays the remaining amount of seconds before completion.
Once the cycle is complete the outer door can be then be opened.
Figure 8: Interlock purge countdown timer